A reliable UHPLC-MS/MS method for the determination of legacy and emerging bisphenol analogues in human urine.

An efficient technique for quantitative analysis of BPA, BPF, BPS, and 11 emerging bisphenol analogues in human urine has been developed, which combined solid phase extraction (SPE) with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Stable isotope internal standards were added to 1.0 mL of urine, which was hydrolyzed by β-glucuronidase overnight, and the target analytes were enriched and purified using an HLB 96-well plate, concentrated to dryness by nitrogen blowdown and reconstituted in 0.

[Determination of 22 organic ultraviolet absorbers in human serum by ultra performance liquid chromatography- tandem mass spectrometry].

In this study， a method for the simultaneous determination of 22 organic ultraviolet absorbers （OUVs） in human serum was established by combining protein precipitation technology （PPT）， efficiency lipid removal technology （ELR） and ultra performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）. The OUVs include five benzophenone compounds， five benzotriazole compounds， two cinnamate ester compounds and their three metabolites， two salicylate compounds， two camphor derivative compounds， one triazine compound， one dibenzoylmethane compound and one amino aminobenzoic acid derivative compound. Chromatography was performed using an Acquity BEH C column （100 mm×2.

The Application of Virus-Induced Gene Silencing in the Study of Rose Petal Abscission.

Virus-induced gene silencing (VIGS) can be used to validate the functional roles of plant genes by infecting plants with viruses carrying target gene segments. In this chapter, we describe VIGS in rose using tobacco rattle virus (TRV) in RNA virus vectors. By silencing the target genes in rose, the roles of related genes in the process of petal abscission can be studied.

Optimization and validation of a multi-residue method for analyzing organic UV absorbers in human urine by UHPLC-MS/MS.

Organic UV absorbers (OUVAs) have gained increasing public concern over the past several years due to their potential adverse effects on humans. The currently available methods for determining OUVAs in humans are typically designed to detect only a limited number of these compounds and lack sufficient sensitivity. In this study, we established a simple and sensitive analytical method that combines liquid-liquid extraction (LLE) with ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) for the determination of 24 OUVAs and metabolites in human urine samples.

[Determination of four oxidative stress biomarkers in human urine using solid-phase extraction coupled with ultra performance liquid chromatography-tandem mass spectrometry].

Oxidative stress biomarkers are measurable biological indicators that reflect the balance between the production of reactive oxygen species (ROS) and the body's ability to neutralize them using antioxidants. Elevated oxidative stress is associated with a number of health effects. Herein, we report the development of a comprehensive and sensitive method for quantifying four typical oxidative stress biomarkers in human urine using solid-phase extraction (SPE) in conjunction with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).

Unravelling the complex influence of dissolved organic matter on microbial diversity in a salinized lake.

Ecosystems in cold and arid regions, such as Dai Lake - a typical inland, salinized lake in the semi-arid region of northern China - face severe environmental challenges, including salinization and biodiversity loss. This study investigates the chemical composition of dissolved organic matter (DOM) and the structure of microbial communities in lake water and sediments, offering novel insights into the ecosystem's dynamics. In winter, DOM in the lake water is primarily derived from decaying plant and animal matter, while sediment DOM is predominantly associated with microbial activity.

Rbfox3 Promotes Transformation of MDSC-Like Tumor Cells to Shape Immunosuppressive Microenvironment.

Myeloid-derived suppressor cells (MDSCs) within the tumor microenvironment (TME) contribute to the malignant progression of tumors by exerting immunosuppressive effects. Bacterial lipopolysaccharides (LPS) have been widely demonstrated in various types of solid tumors. LPS can promote the malignant progression of tumors, which mechanism has not yet been fully elucidated.

Chinese Soil Moisture Observation Network and Time Series Data Set for High Resolution Satellite Applications.

High-quality ground observation networks are an important basis for scientific research. Here, an automatic soil observation network for high-resolution satellite applications in China (SONTE-China) was established to measure both pixel- and multilayer-based soil moisture and temperature. SONTE-China is distributed across 17 field observation stations with a variety of ecosystems, covering both dry and wet zones.