Identification of a tumor microenvironment-related gene signature for predicting prognosis in patients with gastric cancer.

Tumor microenvironment (TME) plays an important role in the prognosis of gastric cancer (GC). The aim of this study was to identify a TME-related gene signature and provide a basis for prognosis evaluation of GC. X-cell and cluster analyses were performed on 373 tumor samples from The Cancer Genome Atlas-Stomach Adenocarcinoma.

Juglone targets MMP-1 to inhibit gastric cancer progression.

The incidence of gastric cancer is increasing annually, and some patients already develop distant metastases by the time of diagnosis. The issues of drug resistance and significant side effects of chemotherapy lead to poor prognosis and pose challenges for clinical treatment. Therefore, developing new therapies for gastric cancer is crucial.

GTSE1-expressed osteoblastic cells facilitate formation of pro-metastatic tumor microenvironment in osteosarcoma.

Understanding metastatic osteosarcoma relies on defining the complexity of cell types, their associated molecular profiles, and interactions among cells in the tumor microenvironment. Here, we integrated single-cell and bulk gene expression datasets and revealed that metastatic lesions were highly enriched for osteoblasts (OB). Under the regulation of E2F family members, OB cells harbored enhanced proliferation activity and high differentiation potential.

Nonemissive Ruthenium Complexes for Near Infrared-II Photodynamic, Photothermal, Sonodynamic, and Sonothermal Therapy Against Drug-Resistant Cancer.

Nine nonemissive Ru(II) complexes have been designed for synergistic NIR-II (1064 nm) photodynamic, photothermal, sonodynamic, and sonothermal therapy targeting cisplatin-resistant nonsmall cell lung cancer. Their quantum yields of singlet oxygen and superoxide anions are significantly elevated under low-power NIR-II laser irradiation or ultrasound treatment. The selected complex completely eradicated drug-resistant lung cancer tumors in all tested mice when treated simultaneously with NIR-II laser and ultrasound at a minimal drug dosage in vivo and was promptly eliminated from the organism.

Comparative transcriptome analysis reveals involvement of FOXL2 in regulation of ovulation cycle in chicken.

The egg-laying performance is closely related to ovulation of chicken. Ovulation is very complex and highly coordinated physiological process. However, little is known about the ovulation-related synergistic actions of molecules and their signaling.

The influence of chronic exposure to violent virtual avatars on players' aggressive behavior: An ERP study.

Short-term exposure to virtual avatars in violent video games has a short-term effect on players' aggressive behavior, but few studies have focused on the effect of chronic exposure to virtual avatars in violent video games on players' aggressive behavior, and its cognitive neural mechanism remains unclear. This study aimed to investigate the effects of chronic exposure to virtual avatars on players' aggressive behavior in violent video games and its cognitive neural mechanism. 54 undergraduates were selected (27 participants with violent virtual avatars exposure) to complete a competitive reaction time task, during which participants' EEG were recorded.

SERCA2 regulates Piezo1 channel activation and contributes to the cardiac function and baroreflex in mice.

Piezo1 channels play important roles in physiological processes such as tactile sensation, blood pressure (BP) control, cardiac development, inflammatory responses as well as in disease processes. Sarco-endoplasmic reticulum Ca-transporting ATPase (SERCA) is the only active protein in the SR that orchestrates calcium homeostasis by translocation of Ca from the cytoplasm to the sarcoplasmic reticulum. It has been shown that SERCA2 inhibits Piezo1 function in mammals by directly acting on the Piezo1 mechano-transduction module of mechanosensitive ion channels.

Shared genetic architecture between stroke and blood lipids: a large-scale genome-wide cross-trait analysis.

Background: Blood lipid levels are linked to stroke risk, but the genetic correlation between blood lipids and stroke is not well understood. We investigated the shared genetic architecture between blood lipids and stroke, including its subtypes, identifying common risk loci, genes, and underlying genetic mechanisms.

Methods: Utilizing large-scale genome-wide association study (GWAS) summary data, we identified genetic overlap between blood lipid levels and stroke.

Insights into zeolite imidazole frameworks (ZIFs) for CO capture and separation: A short review.

Zeolitic imidazole frameworks (ZIFs) are a class of three-dimensional (3D) skeletal materials with zeolitic topology composed of metal ions and imidazolium ligands, which combine the advantages of zeolites and metal-organic frameworks. ZIFs are widely used for adsorption of carbon dioxide (CO) from flue gas due to its excellent hydrophobicity, chemical stability, thermal stability and gas adsorption properties. This study focuses on the effects of structures of ZIFs on CO capture and separation from the viewpoints of topologies, pore channels, ligand functional groups and composite structures.

The Assessment of Subglottic and Cervical Tracheal Stenosis Using a Novel Point-of-Care Ultrasound Method: Measurement of Tracheal Radius Curvature.

Background And Objective: The air way dimensions are important in the assessment, treatment and follow-up of patients with tracheal and subglottic stenosis (SGS). Non-invasive, non-radioactive methods to assess the degree of stenosis are needed. Point-of-care ultrasonography (POCUS) is an effective method for assessing the subglottic diameter and evaluating the abnormal airway.

The RNA-binding E3 ligase MKRN2 selectively disrupts Il6 translation to restrain inflammation.

E3 ligases and RNA-binding protein-mediated dysregulation of proinflammatory cytokines leads to autoimmune and inflammatory diseases. However, whether RNA-binding E3 ligases can regulate specific proinflammatory cytokine expression remains unclear. Here we found that the RNA-binding E3 ligase MKRN2 selectively inhibits the expression of interleukin-6 (IL-6) in lipopolysaccharide-activated macrophages.

METTL3-Driven m6A Modification of Gene in High Fat Diet Related Liver Cancer Tumor Macrophages Facilitates Type II Macrophage Differentiation.

Objective: Obesity induces chronic inflammation and hormonal imbalances that contribute to tumor growth. This study explores the less understood dynamics of tumor-related macrophages under a high-fat diet and its consequent impact on tumor growth, with a focus on elucidating the role of high-fat diets on macrophage behavior in liver cancer.

Methods: We established a mouse obesity model using a high-fat diet, combined with a liver cancer implantation approach.

Pulsed Radiofrequency Relieves Neuropathic Pain by Repairing the Ultrastructural Damage of Chronically Compressed Dorsal Root Ganglion.

Pulsed radiofrequency (PRF) has demonstrated therapeutic potential for neuropathic pain, yet its efficacy in alleviating pain induced by chronic dorsal root ganglion (DRG) compression remains unclear. This study evaluated the analgesic effects of DRG-targeted PRF in a chronic compression of DRG (CCD) rat model. Adult male Sprague Dawley rats were divided into four groups: sham, CCD, CCD+PRF, and CCD+freePRF.

Renalase Overexpression-Mediated Excessive Metabolism of Peripheral Dopamine, DOPAL Accumulation, and α-Synuclein Aggregation in Baroreflex Afferents Contribute to Neuronal Degeneration and Autonomic Dysfunction.

: Increasing evidence reveals the likely peripheral etiology of Parkinson's disease; however, the mechanistic insight into α-Synuclein aggregation in the periphery remains unclear. This study aimed to explore the effect of abnormal expression of renalase on dopamine metabolism, toxic DOPAL generation, and subsequently, α-Synuclein aggregation. : Blood pressure (BP) was monitored while changing the body position of rats; the serum level of renalase was detected by ELISA; the mRNA/protein of renalase and α-Synuclein were determined by qRT-PCR/Western blot; DOPAL was measured using HPLC; renalase distribution was explored by immunostaining; cell viability and ultrastructure were examined by TUNEL and electron microscopy, respectively.

Knockdown of the CALHM1 Gene Alleviates Allodynia in Rats With Trigeminal Neuralgia.

Objective: The sudden onset of trigeminal neuralgia is very similar to epilepsy. CALHM1 is associated with the development of temporal lobe epilepsy. Therefore, does CALHM1 play an important role in the pathogenesis of trigeminal neuralgia? In this study, we explored the effects of pain behaviour in rats with trigeminal neuralgia by knocking down the Calcium homeostasis modulator 1 gene.

Prostaglandin endoperoxide synthase 2 regulates neuroinflammation to mediate postoperative cognitive dysfunction in mice.

Prostaglandin endoperoxide synthase 2 (PTGS2) is a rate-limiting enzyme of prostaglandin (PGs) production, mediating perioperative inflammatory response. This study aimed to explore the mechanisms underlying the involvement of PTGS2 in postoperative cognitive dysfunction (POCD). Transient bilateral common carotid artery occlusion (tBCCAO) was performed to induce POCD.

Genome-Wide identification and expression analysis of CsABF/AREB gene family in cucumber (Cucumis sativus L.) and in response to phytohormonal and abiotic stresses.

Abscisic acid (ABA)-responsive element binding factors (ABF)/ABA-responsive element binding proteins (AREB)/ABA insensitive protein 5 (ABI5) all belong to the basic leucine zipper (bZIP) transcription factor A subfamily. The bZIP transcription factor family contains 13 subfamilies, namely groups A, B, C, D, E, F, G, H, I, J, K, M and S, and the ABF/AREB/ABI5 gene belongs to A subfamily of the bZIP transcription factor. However, genomic analysis of CsABF/AREB in cucumber (Cucumis sativus L.

Dinuclear Ru(II) Complexes for Synergetic Photodynamic, Photothermal, and Sonodynamic Therapy against Cisplatin-Resistant Cancer.

A new series of dinuclear Ru(II) complexes with a novel bridging ligand were developed for the treatment of cisplatin-resistant non-small-cell lung cancer via a synergistic photodynamic, photothermal, and sonodynamic therapy mechanism. Comprehensive experimental and theoretical studies investigated their photophysical and photochemical properties along with the influence of ancillary ligands. The complexes exhibit significant two-photon absorption at the IR region, facilitating ROS generation through both type I and II mechanisms under IR laser and ultrasound exposure.

Ubiquitin Specific Protease 9X Regulates the Activation of ARK5 and Promotes Progression of Fibrotic Remodeling.

USP9X plays a crucial role in myocardial fibrosis. This study showed increased USP9X expression in myocardial infarction models, associated with collagen deposition and myofibroblast activation. Myofibroblast-specific USP9X knockout and pharmacologic inhibition with Degrasyn both reduced fibrosis and improved cardiac function.

The calcium-sensitive receptor in the pathogenesis of sepsis.

Sepsis is an organ dysfunction caused by the body's dysfunctional response to infection, which is one of the most important causes of death in critically ill patients. It is characterized by high morbidity, high mortality, and high treatment costs. Currently, the treatment of sepsis relies mainly on supportive therapy, and there is a lack of targeted intervention ways.

Targeted Anti-Inflammatory Nanozymes with Pro-Angiogenic Activity for Myocardial Infarction Therapy.

Myocardial infarction (MI) poses a significant threat to human health. Current treatments emphasize early revascularization to restore blood supply to the myocardium, often overlooking the extensive oxidative damage and autophagy dysfunction resulting from reactive oxygen species (ROS) release after MI. Therefore, timely and effective interventions to clear ROS in the early stages of MI are crucial for inhibiting the MI pathological progression and restoring cardiac function.

LncRNA PGM5-AS1 inhibits the progression of breast cancer by inhibiting miR-182-5p.

LncRNAs serve as crucial regulators in the survival and proliferation of tumors. This study is dedicated to exploring the functional significance of lncRNA PGM5-AS1 in breast cancer (BRCA). First, the expression level of PGM5-AS1 in BRCA patients and its diagnostic ability for BRCA were analyzed by RT-qPCR and Receiver Operating Characteristic curve.

Biliverdin alleviates cerebral ischemia-reperfusion injury by regulating the P4hb/MAPK/mTOR pathway to inhibit autophagy.

Background: Biliverdin (BV) exhibits anti-inflammatory and antioxidative effects. Autophagy activation is crucial in the pathogenesis of cerebral ischemia-reperfusion injury (CIRI). This study aimed to investigate whether BV could ameliorate CIRI by regulating autophagy.

Interlayer Expanded MXene Film Cathodes with Rich Defects for Flexible 2-Electron Oxalate-Based Li-CO Batteries: A New Path to Enhanced Energy Efficiency and Durability.

Aprotic Li-CO batteries have garnered significant attention owing to their high theoretical energy density and potential in zero-carbon technology. However, their practical application remains hindered by sluggish CO reduction/evolution reaction (CRR/CER) kinetics and limited flexibility. While 2D graphene-like materials are commonly employed to settle these issues, their four-electron pathway limits efficiency and reversibility.