ODC-Driven Polyamines Synthesis Sustains the Self-Renewal of Glioblastoma Stem Cells and Drives Tumor Aggressiveness.

Glioblastoma stem cells (GSCs) are crucial drivers of tumor progression and therapeutic resistance in glioblastoma multiforme (GBM), yet the molecular mechanisms maintaining their self-renewal remain incompletely understood. Here, we identify a critical role for polyamine metabolism in GSCs maintenance. We show that GSCs exhibit elevated polyamine levels compared to non-stem tumor cells, attributed to enhanced ornithine decarboxylase (ODC) expression.

Preparation and characterization of biomineralizing dentin bonding primer based on bioactive amphiphilic raspberry-like nanoparticles.

Background: Incomplete resin coverage of demineralized collagen can compromise dentin bond durability. This study developed a biomineralizing dentin bonding primer incorporating bioactive amphiphilic raspberry-like nanoparticles (BRPs) to enhance biomineralization and dentin bond strength.

Methods: BRPs were characterized using scanning electron microscopy (SEM) and nitrogen (N) adsorption experiments.

Mitochondrial Calcium Uniporter Links Acetyl-CoA Metabolism and H3K27 Acetylation to Maintain Glioblastoma Stem Cells.

Glioblastoma stem cells (GSCs) exhibit remarkable metabolic and epigenetic adaptability, contributing to therapeutic resistance and tumor recurrence. The mechanisms underlying this plasticity represent potential targetable vulnerabilities to improve glioblastoma treatment. Here, we identified a critical metabolic-epigenetic axis centered on the mitochondrial calcium uniporter (MCU) that governs GSC survival and tumor initiation.

Mechanistic Insights into the Contrasting Effects of Low DO on Nitrification-Derived NO Emissions in Comammox - versus AOB-Dominated Sludge.

Nitrification significantly contributes to NO emissions in wastewater treatment, typically enhanced at low dissolved oxygen (DO). The present study revealed that low DO (∼0.2 mg/L) enhanced the NO emission factor (EF) from nitrification by 4.

Elevated nonhomologous end-joining by AATF enables efficient DNA damage repair and therapeutic resistance in glioblastoma.

Glioblastoma (GB) is a highly aggressive brain tumor resistant to chemoradiotherapy, largely due to glioma stem-like cells (GSCs) with robust DNA damage repair capabilities. Here we reveal that GSCs enhance their DNA repair capacity by activating non-homologous end-joining (NHEJ) through upregulation of the apoptosis antagonizing transcription factor (AATF), thereby promoting therapeutic resistance in GB. AATF interacts with XRCC4, a core NHEJ subunit, preventing its degradation via ubiquitin-mediated proteasomal processes.

Central Role of Hypertension in HIV Comorbidity Networks: A Population-Based Study of Age and Sex-Specific Patterns in Southwest China.

Background: The increasing life expectancy among people living with HIV (PLWH) has transformed HIV management into chronic disease care. This transformation has introduced complex patterns of comorbidities that challenge current health care approaches. A deeper understanding of the interactions between these conditions and their distribution across demographic groups remains essential for optimizing care.

Fabricating a novel bioactive resin Infiltrant to treat white spot lesions of enamel.

Objective: This study aims to develop a novel bioactive resin infiltrant (BRI) to treat white spot lesions (WSLs) of enamel.

Methods: BRI was prepared by adding an appropriate amount of high-phosphorus bioactive glass named as PSC (10.8 mol% PO-54.

The RNA-Binding Proteins MCPIP2 and IGF2BP1 Competitively Modulate Breast Tumor Angiogenesis by Antagonizing VEGFA mRNA Stability and Expression.

Tumor angiogenesis is essential for further growth and metastasis of solid tumors. However, the mechanisms underlying angiogenesis-related gene expression have yet to be clarified. Here, we discovered RNA-binding proteins monocyte chemotactic protein-induced protein 2 (MCPIP2) and insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) function as a pair of antagonists that modulate breast tumor angiogenesis by competitively regulating mRNA stability of proangiogenic gene transcripts, including vascular endothelial growth factor A (VEGFA), Erb-B2 receptor tyrosine kinase 2 (ERBB2), interleukin-8 (IL8), C-X-C motif chemokine ligand 1 (CXCL1), and ephrin A1 (EFNA1).

SlDCD and SlLCD increased the salt tolerance in tomato seedlings by enhancing antioxidant and photosynthesis capacity.

Using gene silence and heterologously overexpression, hydrogen sulfide synthesis-related genes l-cysteine desulfhydrase and d-cysteine desulfhydrase have been shown to enhance salt tolerance in tomato seedlings. Hydrogen sulfide (HS) plays an important role in alleviating abiotic stress. L-Cysteine desulfhydrase (LCD) and D-cysteine desulfhydrase (DCD) are two important HS synthesis enzymes.

Genome-Wide identification and expression analysis of CsABF/AREB gene family in cucumber (Cucumis sativus L.) and in response to phytohormonal and abiotic stresses.

Abscisic acid (ABA)-responsive element binding factors (ABF)/ABA-responsive element binding proteins (AREB)/ABA insensitive protein 5 (ABI5) all belong to the basic leucine zipper (bZIP) transcription factor A subfamily. The bZIP transcription factor family contains 13 subfamilies, namely groups A, B, C, D, E, F, G, H, I, J, K, M and S, and the ABF/AREB/ABI5 gene belongs to A subfamily of the bZIP transcription factor. However, genomic analysis of CsABF/AREB in cucumber (Cucumis sativus L.

Real experience of young and middle-aged hemodialysis patients: a qualitative meta-synthesis.

Background: About 89% of global End-stage renal disease (ESRD) patients receive hemodialysis. Data show that about 40% ~ 60% of dialysis patients are young and middle-aged. Hemodialysis significantly impacts the daily life and rehabilitation of patients, underscoring the urgency of understanding their experiences and needs.

Comparison of Fluorescence In Situ Hybridization, Next-Generation Sequencing, and DNA Methylation Microarray for Copy Number Variation Assessment in Gliomas.

Gene-level and chromosomal copy number variation (CNV) assessments are critical in the integrated diagnosis of gliomas. Whereas fluorescence in situ hybridization (FISH) has been traditionally employed for CNV detection, emerging technologies such as next-generation sequencing (NGS) and DNA methylation microarray (DMM) are available in clinical practice. Nevertheless, the comparative performance of these 3 assays and the concordance of them remain unclear.

Asymmetric spillover connectedness between clean energy markets and industrial stock markets: How uncertainties affect it.

As the global climate crisis intensifies, clean energy is becoming increasingly important, and the intrinsic link between industry and energy highlights the connectedness between the industrial stock market and the clean energy market, and examining this connectedness can reveal risk spillovers between these markets. We categorise the clean energy market into hydro, wind and solar markets, and the industrial stock market into low-carbon portfolios, high-carbon portfolios and ordinary portfolios, and use the network connectedness methodology to investigate the connectedness of returns between the clean energy submarkets and the industrial stock submarkets in the time and frequency domains. The returns are categorised into positive and negative returns in order to investigate the asymmetry in the connectedness of the markets.

Petroselinic Acid from Apiaceae Family Plants Ameliorates Autoimmune Disorders Through Suppressing Cytosolic-Nucleic-Acid-Mediated Type I Interferon Signaling.

The recognition of cytosolic nucleic acids is a critical step in the host immune response against danger signals, such as molecular patterns from pathogens or tissue damage. Nonetheless, over-reactivity to self-nucleic acids leads to the sustained production of type I interferon (IFN), mediated either by cGAS or RLR, contributing to the pathogenesis of certain autoimmune diseases, such as Aicardi-Goutières syndrome (AGS). Therefore, inhibiting excessive IFN production represents a potential therapeutic strategy for such autoimmune conditions.

Liquid-liquid phase separation of LARP7 restrains HIV-1 replication.

HIV-1 initiates replication by its transactivator Tat, hijacking the positive transcription elongation factor b (P-TEFb) in the host cell. Most P-TEFb is maintained in an inactive state by 7SK snRNP until it is brought to the transcription initiation complex by cellular or viral transactivators that accelerate transcription and facilitate the production of full-length viral transcripts. Here, we report that HIV-1 infection triggers liquid-liquid phase separation of LARP7, a central component of 7SK snRNP.

Interdisciplinary perspectives on diabetes and microcirculatory dysfunction: A global bibliometric analysis.

Background: The prevalence of diabetes and its association with microcirculatory dysfunction presents a significant challenge in contemporary global health. Addressing this nexus is crucial for developing targeted therapeutic interventions.

Aim: To trace the progression and delineate the current state of interdisciplinary research concerning diabetes and microcirculation.

Discovery of 3-indolylbenzoquinone derivatives with therapeutic potential for breast cancer.

Breast cancer is one of the most prevalent malignant tumors in women, but the side effects and drug resistance limit the long-term effectiveness of existing drugs. To address these issues, we designed and synthesized a series of novel mono- and bis-indole-substituted 3-indolylbenzoquinone derivatives and evaluated their inhibitory activity against breast cancer. Among them, compound 1b demonstrated the most potent inhibitory activity against the MDA-MB-231 breast cancer cell line (IC = 3.

Berberine is a Novel Mitochondrial Calcium Uniporter Inhibitor that Disrupts MCU-EMRE Assembly.

The mitochondrial calcium uniporter (MCU) complex mediates Ca entry into mitochondria, which plays a crucial role in regulating cellular energy metabolism and apoptosis. Dysregulation of MCU is implicated in various diseases, such as neurodegenerative disorders, cardiac diseases, and cancer. Despite its importance, developing specific and clinically viable MCU inhibitors is challenging.

Cognitive frailty in maintenance hemodialysis: a scoping review.

Purpose: To conduct a scoping review of the related research on cognitive frailty (CF) in maintenance hemodialysis (MHD) patients, so as to provide a basis for early diagnosis, treatment and intervention of CF in MHD patients.

Methods: Utilizing a scoping review approach, we searched PubMed, Embase, The Cochrane Library, Web of Science, CINAHL, the China Biological Medicine Database (CBM), China National Knowledge Infrastructure (CNKI), Wanfang, and Weipu (VIP) for literature on CF in MHD patients up to October 20, 2024. Two researchers conducted independent screening and data extraction of the literature's fundamental characteristics.

FSD1 inhibits glioblastoma diffuse infiltration through restriction of HDAC6-mediated microtubule deacetylation.

The infiltration of glioblastoma multiforme (GBM) is predominantly characterized by diffuse spread, contributing significantly to therapy resistance and recurrence of GBM. In this study, we reveal that microtubule deacetylation, mediated through the downregulation of fibronectin type III and SPRY domain-containing 1 (FSD1), plays a pivotal role in promoting GBM diffuse infiltration. FSD1 directly interacts with histone deacetylase 6 (HDAC6) at its second catalytic domain, thereby impeding its deacetylase activity on α-tubulin and preventing microtubule deacetylation and depolymerization.

Genome-Wide Identification and Expression Analysis of the Gene Family in Roses ( Jacq.).

Calmodulin-binding transcription activator (), as one of the transcription factors, is involved in performing important functions in modulating plant stress responses and development in a Ca/CaM-driven modus. However, genome-scale analysis of has not been systemically investigated in roses. Rose ( Jacq.

Predictive Factors of ART Follow-Up Loss in HIV Patients (2018-2022): A Retrospective Cohort Study.

Loss to follow-up (LTFU) in antiretroviral therapy (ART) poses significant challenges in the management of HIV/AIDS. This study aims to identify socio-demographic and clinical factors influencing LTFU among patients undergoing ART in Luzhou, China, and to develop a predictive model for LTFU using Cox risk regression analysis. In this retrospective cohort study, data from 8,770 patients diagnosed with HIV infection between January 1, 2018 and December 31, 2022 who were enrolled in the national free ART program were analyzed.

NCAM1 modulates the proliferation and migration of pulmonary arterial smooth muscle cells in pulmonary hypertension.

Background: Pulmonary hypertension (PH) is a malignant vascular disease characterized by pulmonary arterial remodeling. Neural cell adhesion molecule 1 (NCAM1) is a cell surface glycoprotein that is involved in a variety of diseases, including cardiovascular disease. However, the role of NCAM1 in PH remains underexplored.

Effect of histone modifications on fruit ripening.

Histone modifications are canonical epigenetic modifications mediating plant growth and development. Specially, histone modifications play important regulatory roles in plant fruit ripening, directly affecting fruit color changes, soluble sugar accumulation, and fruit softening. In this review, we focus on the effects of histone acetylation and methylation during fruit ripening.

CsLCD and CsDCD are involved in nitric oxide-enhanced salt tolerance in cucumber seedlings.

Nitric oxide (NO) and hydrogen sulphide (HS) play important roles in plant growth, development and environmental adaptation. Currently, there is few information on the mechanism by which HS may be involved in NO-induced salt tolerance. In this study, cucumber was used to investigate the role of NO and HS and their relationship under salt stress.