Piezo1 in heart failure: A new perspective from cytomechanical sensing to diverse cellular pathways.

Piezo1, a mechanosensitive cation channel characterized by its distinctive transmembrane trimeric structure, plays a pivotal role in mediating cellular responses to mechanical stimuli. It facilitates calcium influx, and recent studies highlight its involvement in heart failure (HF) pathophysiology, where its dysregulation significantly contributes to disease progression. Specifically, Piezo1 upregulation impacts diverse cellular processes across cardiovascular cell types, including cardiomyocytes, fibroblasts, endothelial cells (ECs), and vascular smooth muscle cells (VSMCs).

NaviNeRF++: Towards Interpretable 3D Reconstruction via Unsupervised Disentangled Representation Learning.

3D reconstruction is a pivotal technology that recreates three-dimensional structures from two-dimensional representations, facilitating AI's understanding and interaction with the real world. However, existing methods pose challenges from two perspectives, i.e.

Farrerol confers neuroprotection in spinal cord injury by regulating macrophages/microglia polarization through the JAK2/STAT3 pathway.

Background And Aims: Spinal cord injury (SCI) causes secondary damage characterized by neuroinflammation and imbalanced macrophages/microglia polarization, worsening neuronal loss and functional decline. Farrerol (FAR), a natural flavonoid with anti-inflammatory properties, has not been studied for SCI treatment. This work assesses FAR's neuroprotection through macrophages/microglia polarization regulation and explores its mechanisms.

Do non-bone metabolism laboratory indicators differ between men and women with osteoporosis?: A retrospective study based on physical examination data.

With the global population aging, osteoporosis has emerged as a significant health concern worldwide. Osteoporotic fractures predominantly occur in the forearm, hip, and lumbar spine, and are associated with substantial morbidity and mortality. Osteoporosis is a multifactorial disease, and prior research has identified several clinical risk factors, including advanced age, gender, weight, history of fractures, smoking, alcohol abuse, and corticosteroid use.

OccScene: Semantic Occupancy-based Cross-task Mutual Learning for 3D Scene Generation.

Recent diffusion models have demonstrated remarkable performance in both 3D scene generation and perception tasks. Nevertheless, existing methods typically separate these two processes, acting as a data augmenter to generate synthetic data for downstream perception tasks. In this work, we propose OccScene, a novel mutual learning paradigm that integrates fine-grained 3D perception and high-quality generation in a unified framework, achieving a cross-task win-win effect.

Investigating the Effect of Hydrogen Bonding on the Viscosity of an Aqueous Methanol Solution Using Raman Spectroscopy.

Water science has always been a central part of modern scientific research. In this study, the viscosity and hydrogen bond structures of methanol aqueous solutions with different molar ratios were investigated via confocal microscopic Raman spectroscopy. The Raman spectra of methanol in the CH and CO stretching regions were measured in order to investigate the structure of water/methanol molecules.

Preparation of Glass-Ceramics Using Zinc-Containing Smelting Slag: Structure, Properties and Solidification of Zinc.

The stabilization of heavy metal elements, such as zinc, in the form of ions within the glass-ceramics represents a valuable approach to addressing environmental pollution caused by heavy metals. This study investigates the feasibility and physicochemical properties of diopside-based glass-ceramics synthesized from zinc-containing smelting slag. The zinc-rich smelting slag is abundant in SiO, AlO, CaO, and other constituents, thereby providing cost-effective and efficient raw materials for glass-ceramic production.

Self-splicing RNA circularization facilitated by intact group I and II introns.

Circular RNA (circRNA) has gained significant attention in RNA therapeutics due to its enhanced stability and protein-coding potential. In this study, we present two in vitro RNA circularization techniques, namely Permuted Intron-Exon through Trans-splicing (PIET) and Complete self-splicing Intron for RNA Circularization (CIRC). PIET leverages the second step of group I intron splicing, offering an alternative circularization strategy.

Impact of cyclosporine A-related single nucleotide polymorphisms on post-transplant outcomes in pediatric hematologic malignancy patients undergoing allogeneic hematopoietic stem cell transplantation.

Background: Calcineurin inhibitors (CNIs), such as cyclosporine A (CsA), are widely used as immunosuppressants for both prophylactic and therapeutic purposes in patients with graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT). CsA-related transporters and metabolic enzymes single nucleotide polymorphisms (SNPs) are associated with the efficacy of CsA in individuals. However, few studies have explored how CsA-related SNPs correlate with post-transplant complications and prognosis.

Medicaid Managed Care: Substantial Shifts In Market Landscape And Acquisitions, 2006-20.

There has been a significant shift in the composition of the Medicaid managed care market over the course of the past two decades. The market is consolidated among five national firms, and those firms have nearly half of all beneficiaries enrolled in managed care plans. State markets are even more consolidated, typically giving Medicaid beneficiaries only a few plan options.

Revealing Performance-Limiting Buried Interfaces in Layered Dion-Jacobson Lead-Iodide Perovskites.

Dion-Jacobson (DJ) two-dimensional (2D) perovskites have emerged as promising photovoltaic materials due to their superior structural and chemical stability compared to three-dimensional (3D) perovskites. However, their optoelectronic performance remains limited by imperfections at interfaces and a fundamental understanding of these interfacial properties is missing. Herein, we present interfacial structure-property relationships in DJ perovskites by directly probing buried interfaces using combined spectroscopic and microscopic characterization techniques.

Efficient and wavelength-tunable second-harmonic generation toward the green gap.

Achieving compact and efficient visible laser sources is crucial for a wide range of applications. However, traditional semiconductor laser technology faces difficulties in producing high-brightness green light, leaving a "green gap" in wavelength coverage. Second-harmonic generation (SHG) offers a promising alternative by converting near-infrared sources to visible wavelengths with high efficiency and spectral purity.

The m5C reader Alyref regulates cardiac remodeling post-myocardial infarction by modulating extracellular matrix protein synthesis in cardiac fibroblasts.

Myocardial infarction (MI) triggers a significant injury response that eventually leads to adverse cardiac remodeling and heart failure, with the extracellular matrix, including collagen, plays a crucial role in this process. However, the relationship between 5-methylcytosine (m5C) modification and cardiac remodeling after MI remains unclear. In this study, an MI model was established in mice through ligation of the left anterior descending coronary artery, and hypoxia-induced primary neonatal cardiac fibroblasts were used as a cell model.

Triptonide facilitates autophagy-mediated apoptosis in esophageal squamous cell carcinoma by targeting the AMPK-mTOR-ULK1 axis.

Triptonide (TN) is a small-molecule compound initially derived from Hook. f used in traditional Chinese medicine. However, its potential antitumor mechanisms are still far from adequately understood.

Efficacy and safety of rabbit ATLG and ATG in allogeneic hematopoietic stem cell transplantation for children with acquired severe aplastic anemia.

Severe aplastic anemia (SAA) is a life-threatening bone marrow failure syndrome characterized by bone marrow hypoplasia and peripheral blood cytopenia. Without timely treatment, it frequently proves fatal. Rabbit anti-thymocyte globulin (ATG) and anti-human T lymphocyte globulin (ATLG) are widely used for graft-versus-host disease (GVHD) prophylaxis.

State of the art indicators for imaging purinergic dynamics in vitro and in vivo.

Purinergic neurotransmission, a dynamic signaling system using adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine (ADO), uridine diphosphate (UDP), and others, plays a crucial role in brain function. Purinergic signaling is involved in regulating synaptic communication to influence sleep and neuroprotection; malfunction of purinergic signaling contributes to various neurological disorders like pain, epilepsy, and depression. Effective detection methods are crucial for a comprehensive understanding of the multifaceted roles of purinergic signaling in the brain.

Bolus Use in Postmastectomy Radiation Therapy for Breast Cancer: A Systematic Literature Review.

IntroductionTissue-equivalent boluses are used to increase the skin surface dose in patients with breast cancer undergoing postmastectomy radiotherapy. Boluses made from various materials have been developed and applied in clinical practice. However, there is currently no international standardization for their use.

Pharmacological characterisation of allosteric modulators at human mGlu.

The metabotropic glutamate receptor 5 (mGlu) is a Class C G protein-coupled receptor, ubiquitously expressed throughout the central nervous system (CNS). With major roles in cognition, learning and memory, mGlu dysfunction is linked with numerous neurodegenerative and neuropsychiatric disorders, representing a viable therapeutic target. Allosteric modulators bind topographically distinct sites from glutamate and other orthosteric agonists and enhance (positive allosteric modulators, PAMs), inhibit (negative allosteric modulators, NAMs) or do not effect (neutral allosteric ligands, NALs) mGlu function.

The dosimetric value and safety evaluation of 3D printed bolus in adjuvant intensity-modulated radiotherapy after radical mastectomy for breast cancer: a prospective cohort study.

Purpose: Breast cancer patients may use a bolus to increase the dose to skin in radiotherapy after radical mastectomy. The 3D printed bolus (3DPB) specifically customized based on individual conditions offers better conformity. This study aims to provide clinical insights by evaluating the dosimetric benefits and safety of 3DPB in radiotherapy following radical mastectomy for breast cancer.

Elevated nonhomologous end-joining by AATF enables efficient DNA damage repair and therapeutic resistance in glioblastoma.

Glioblastoma (GB) is a highly aggressive brain tumor resistant to chemoradiotherapy, largely due to glioma stem-like cells (GSCs) with robust DNA damage repair capabilities. Here we reveal that GSCs enhance their DNA repair capacity by activating non-homologous end-joining (NHEJ) through upregulation of the apoptosis antagonizing transcription factor (AATF), thereby promoting therapeutic resistance in GB. AATF interacts with XRCC4, a core NHEJ subunit, preventing its degradation via ubiquitin-mediated proteasomal processes.

3D hydrogel platform with macromolecular actuators for precisely controlled mechanical forces on cancer cell migration.

Mechanical forces play a critical role in regulating cancer cell behavior, particularly during metastasis. Here we present a three-dimensional hydrogel platform embedded with near-infrared-responsive macromolecular actuators that enable precise mechanical stimulation of specific integrin subtypes in cancer cells. By leveraging this system, we investigate how different force parameters-magnitude, frequency, and duration-affect the migration and invasion of ovarian cancer cell spheroids, focusing on the integrins αvβ3 and αvβ6.

Fabricating 3D Network for FeP@MXene toward Stable and High-Capacity Lithium-Ion Storage.

The designing and searching superior anode materials with low operation potential and rapid redox kinetics is of paramount importance. Incorporating transition metal (TM) into phosphorus to form TM phosphides and combining them with low-dimension materials represents effective strategy for enhancing the electrochemical performances. Herein, a 3D network FeP@MXene composite anode is proposed with exhibiting a high reversible capacity of 444.

Lysionotin promoted apoptosis of hepatocellular carcinoma cells via inducing autophagy.

Background: Hepatocellular carcinoma is a prevalent malignant tumor with a high mortality rate. Natural plants hold promise for its treatment, however, the mechanism of lysionotin induced apoptosis in liver cancer cells unclearly. This study aims to investigate the microenvironment alterations and the efficacy of lysionotin in liver cancer.

Advancing personalized, predictive, and preventive medicine in bladder cancer: a multi-omics and machine learning approach for novel prognostic modeling, immune profiling, and therapeutic target discovery.

Objective: This study aimed to identify and analyze immunogenic cell death (ICD)-related multi-omics features in bladder cancer (BLCA) using single-cell RNA sequencing (scRNA-seq) and bulk RNA-seq data. By integrating these datasets, we sought to construct a prognostic signature (ICDRS) and explore its clinical and biological implications, including its association with immune cell infiltration, tumor microenvironment (TME), and drug sensitivity.

Methods: Publicly available datasets from TCGA and GEO, including scRNA-seq (GSE222315, 9 samples) and bulk RNA-seq (TCGA-BLCA, 403 samples; GSE13507, 160 samples), were analyzed.

Brief verbal intervention to address inappropriate prescriptions of chinese patent medicines among western practitioners in primary health care (BRAVERY): a study protocol for an unannounced standardized patient experiment with a factorial design randomized controlled trial in China.

Introduction: Chinese patent medicines, produced using advanced pharmaceutical techniques and available in various forms, including powders, granules, tablets, pills, and capsules, finds extensive utilization among Western medicine practitioners in primary healthcare (WMP-PHC). However, the inappropriate overprescribing of these medicines has led to significant resource waste and raised considerable concerns. Therefore, we aim to address related knowledge gaps by employing unannounced standardized patients (USPs) as a method, for both measurement and intervention research.