Development and validation of a comprehensive risk warning indicator system for depression in Chinese children and adolescents: A cross-sectional observational study.

Depression among children and adolescents has reached epidemic proportions globally, necessitating systematic early detection approaches. Existing screening instruments demonstrate variable accuracy across populations and lack comprehensive and culturally appropriate frameworks for Chinese pediatric populations. This study developed and validated a comprehensive risk warning indicator system for depression detection in Chinese children and adolescents.

Shared and distinct alterations of thalamic subregional functional connectivity in early- and late-onset obsessive-compulsive disorder.

Background: Studies highlight the thalamus as a key region distinguishing early- from late-onset obsessive-compulsive disorder (OCD). While structural thalamic correlates with OCD onset age are well-studied, resting-state functional connectivity (rsFC) remains largely unexplored. This study examines thalamic subregional rsFC to elucidate pathophysiological differences in OCD based on different onset times.

A genome-scale drug discovery pipeline uncovers therapeutic targets and a unique p97 allosteric binding site in .

Schistosomes are parasitic flatworms that infect more than 200 million people globally. However, there is a shortage of molecular tools that enable the discovery of potential drug targets within schistosomes. Thus, praziquantel has remained the frontline treatment for schistosomiasis despite known liabilities.

A repurposed AMP binding domain reveals mitochondrial protein AMPylation as a regulator of cellular metabolism.

Protein AMPylation, the covalent addition of adenosine monophosphate (AMP) to protein substrates, has been known as a post translational modification for over 50 years. Research in this field is largely underdeveloped due to the lack of tools that enable the systematic identification of AMPylated substrates. Here, we address this gap by developing an enrichment technique to isolate and study AMPylated proteins using a nucleotide-binding protein, hinT.

Gut microbial metabolite TMAO impairs cognitive function and induces hippocampal synaptic plasticity decline through modulation of GSK-3β activity.

Background And Objectives: Growing evidence has suggested that elevated Trimethylamine N-oxide (TMAO) levels, a gut microbiota-dependent metabolite, are closely associated with brain aging and cognitive impairment. Glycogen synthase kinase-3 beta (GSK-3β) activity was depicted to be essential in regulating learning and memory. The current study examined the impact of TMAO on cognitive function in mild cognitive impairment (MCI) patients and rat models while exploring the mechanisms regulating the TMAO-induced GSK-3β signaling.

Divergent residues influence PHYTOENE SYNTHASE isozyme interactions with GGPPS and the resulting enzyme activities in peach.

Carotenoids serve critical biological functions through their essential contributions to organismal survival and health. As a widely consumed fruit species, peach (Prunus persica) provides humans with valuable carotenoid sources, and these compounds also substantially enhance the aromatic properties of peach fruits. The synthesis of phytoene, catalyzed by phytoene synthase (PSY), constitutes a key rate-limiting step in carotenoid biosynthesis.

Metal-Organic Framework-Derived Hierarchically Heterostructured ZnCoO/ZnO Composites for High-Performance Supercapacitors.

Three-dimensional hierarchically structured ZnCoO/ZnO (ZnCoO) composites have been prepared through a simple thermal treatment of ZnCo-bimetal MOF (ZnCo-MOF) precursors. We found that the solvothermal reaction temperature for preparing ZnCo-MOFs has an important influence on the composition and structure of the ZnCoO composites. When the temperature is 100 °C, ZnCoO-100 and ZnCoO-100-P (PVP added) present well-defined hierarchical cubic structures composed of loosely packed nanoplates and nanorods, in which ZnO is the major component.

Geometric-Aware Low-Light Image and Video Enhancement via Depth Guidance.

Low-Light Enhancement (LLE) is aimed at improving the quality of photos/videos captured under low-light conditions. It is worth noting that most existing LLE methods do not take advantage of geometric modeling. We believe that incorporating geometric information can enhance LLE performance, as it provides insights into the physical structure of the scene that influences illumination conditions.

Vinyl Acetate-Enhanced Polyvinyl Chloride Gel with High Electroadhesion and Self-Heating-Tunability for Soft Robots in Freezing Environments.

Polyvinyl chloride gel (PVCg) exhibits versatile electromechanical properties, making it highly promising for soft robots. However, conventional PVCg with excessive plasticizers generates a significant amount of heat and suffers from premature electrical breakdown during electro-induced actuation, seriously limiting its widespread application. Here, a novel strategy is demonstrated to simultaneously regulate the heat generation and improve the electromechanical properties of PVCg by introducing polyvinyl chloride-co-vinyl acetate (PVCVA) to fabricate PVCVA gel (PVCVAg).

Comparative longitudinal analysis of pulmonary function post-pediatric Allo-HSCT: benign vs. malignant diseases and early predictors.

Pulmonary complications are a major cause of morbidity following pediatric allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, longitudinal pulmonary function test (PFT) trajectories and early predictors of dysfunction, particularly the interplay between underlying disease, PFTs, and graft-versus-host disease (GVHD), remain poorly understood. In this retrospective single-center study, we analyzed 233 children undergoing their first allo-HSCT with ≥ 100-day survival.

Comparison of totally extraperitoneal versus Lichtenstein operation under local or general anesthesia for inguinal hernia: a meta-analysis.

Purpose: This meta-analysis compared totally extraperitoneal (TEP) laparoscopic repair with open Lichtenstein repair performed under local or general anaesthesia for unilateral inguinal hernia, asking whether the surgical or anaesthetic approach affects postoperative safety, pain and functional recovery.

Methods: PubMed, Embase, Cochrane and Web of Science were searched to 1 April 2025. Eighteen randomized and observational studies comprising 4302 patients met predefined inclusion criteria.

Treatment of acute intracerebral haemorrhage with a Chinese herbal formula (Shengdi Dahuang Decoction): a multicentre, double-blind, randomised, placebo-controlled trial.

Importance: Per preliminary studies, Shengdi Dahuang Decoction (SDD) is potentially effective for acute intracerebral haemorrhage (ICH); however, its effectiveness has not been rigorously assessed in extensive randomised clinical trials.

Objective: To evaluate whether SDD can improve 90-day functional outcomes in patients with ICH.

Design: Randomised, double-blind, placebo-controlled clinical trial included patients with acute ICH within 4 hours of symptom onset at five hospitals in Shanghai, China.

Posterior circulation infarction with sudden deafness as a clinical manifestation.

Hearing loss caused by stroke must be taken seriously, as it is a treatable condition that highly depends on timely intervention. This case report presents a rare case of a 75-year-old female with a history of hypertension who visited the Emergency Department of Otolaryngology due to sudden left-sided deafness accompanied by vertigo and vomiting, and magnetic resonance imaging (MRI) demonstrated acute posterior circulation cerebral infarction.In the Emergency Department setting, for middle-aged and elderly patients with vascular risk factors, hearing loss may serve as an early warning sign of vertebrobasilar artery ischemic stroke.

Integrin β3E726 Regulates the Switch Between Platelet Spreading and Clot Retraction by Interfering Gα13/RhoA Pathway.

Platelet spreading and clot retraction, albeit both mediated by integrin outside-in signaling, lead to platelet shape changes in two opposite directions. The mechanisms by which these processes are regulated are not fully understood. Our previous study found that E726Q mutation in β3 integrin caused impaired spreading in Chinese hamster ovary (CHO) cells on immobilized fibrinogen.

Exploratory analysis of potential association between oral and sleep disturbances in major depressive disorder patients.

Background: The current study aimed to explore the specific oral microbiota profiles in major depressive disorder (MDD) patients with sleep disturbances, and to evaluate the potential mechanisms by which oral microbiota may be implicated in MDD.

Method: Thirty-eight MDD patients experiencing sleep disturbances and thirty healthy controls (HCs) were included. All MDD patients underwent a 14-day antidepressive treatment regimen.

Analysis of the Network Pharmacology Mechanism and Clinical Efficacy of Qingre Jiedu Decoction (QJD) in the Treatment of Infectious Mononucleosis (IM) in Children.

Purpose: This study aimed to clarify the molecular mechanisms of Qingre Jiedu Decoction (QJD), a traditional Chinese medicine (TCM), in treating infectious mononucleosis (IM). By combining network pharmacology and clinical trial validation, the research sought to understand how QJD interacts with biological pathways to combat IM.

Methods: The research team identified active compounds and their targets in QJD using databases like TCMSP and STITCH.

Paeonol protects against cisplatin-induced premature ovarian failure via anti-inflammatory and antioxidant activities: an integrated approach of network pharmacology, molecular dynamics simulation, and experimental validation.

Cisplatin (CIS), a widely used platinum-based chemotherapeutic agent, often induces ovarian toxicity that severely threatens female reproductive health. Paeonol (PAE), a bioactive compound found in peony, exhibits multiple pharmacological activities. This study investigates the multitarget protective mechanisms of PAE against cisplatin-induced premature ovarian failure (POF) using network pharmacology, molecular docking, and molecular dynamics simulations.

Genetic Associations of Clonal Hematopoiesis With Cardioembolic Stroke: Insights From Genome-Wide Mendelian Randomization, Bulk RNA, Single-Cell RNA Sequencing.

Aims: Ischemic stroke (IS), a major global health concern, is associated with aging-related clonal hematopoiesis of indeterminate potential (CHIP), though their mechanistic connection remains unclear. This study explores the causal CHIP-IS relationship, key genetic drivers, and potential therapies.

Methods: Genetic markers for CHIP were selected as instrumental variables and analyzed through bidirectional two-sample Mendelian randomization (MR) using GWAS data from IS cohorts.

Movement-related activity in the internal globus pallidus of the parkinsonian macaque.

Although the basal ganglia (BG) play a central role in the motor symptoms of Parkinson's disease, few studies have investigated the influence of parkinsonism on movement-related activity in the BG. Here, we studied the perimovement activity of neurons in globus pallidus internus (GPi) of nonhuman primates (NHPs) during performance of a choice reaction time reaching task before and after the induction of parkinsonism by administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Neuronal responses, including increases or decreases in firing rate, were equally common in the parkinsonian brain as seen before MPTP, and the distribution of different response types was largely unchanged.

Neurofibromatosis Type 1 with Relapsed/Refractory Precursor B-Lymphoblastic Lymphoma: Case Report and Literature Review.

Introduction: Concomitant occurrence of B-lymphoblastic lymphoma (B-LBL) and neurofibromatosis type 1 (NF1) is rare.

Case Presentation: We diagnosed and treated a child presenting with NF1 and relapsed B-LBL and reviewed the relevant literature through the last 8 years. Stage IV precursor B-LBL with central nervous system 3 was identified in this patient, with pain and activity abnormalities in both lower limbs.

A multi-task deep neural network reveals inflowing river impacts for predictive lake management.

Lake ecosystems, vital freshwater resources, are increasingly threatened by pollution from riverine inputs, making the management of these loads critical for preventing ecological degradation. Predicting the combined effects of multiple rivers on lake water quality is a significant challenge; traditional mechanistic models are computationally intensive and data-dependent, while conventional machine learning methods often fail to capture the system's multifaceted nature. This complexity creates a critical need for an integrated predictive tool for effective environmental management.

Excellent patient-reported long-term quality of life after an Ivor Lewis esophagectomy for cancer.

Background: Long-term health-related quality of life (HRQOL) may be impacted by upper gastrointestinal tract dysfunction following esophagectomy in up to two-thirds of patients. After esophagectomy, the stomach conduit is passively relying on gravity for drainage. Any resistance to flow through the stomach conduit, therefore, has the potential to significantly impact long-term HRQOL.