GNAS/PKA signaling promotes aberrant osteochondral differentiation of Gli1 tendon sheath progenitors.

Tendon injury promotes aberrant osteochondral differentiation of tendon stem cells (TSCs) and results in disability. However, the cellular subsets within the osteochondral lineage involved in this process and associated mechanisms remain unclear. Here, we found that, following Achilles tenotomy, murine Gli1 tendon sheath cells expanded rapidly, transitioning into tenogenic and osteochondrogenic cells.

Clinical and pathological features of 209 cases of pilomatricoma in children: a retrospective study.

Background: Pilomatricoma, albeit benign, may have a potential impact on children's appearance and health. Clinical practice has revealed variations in the clinical manifestations, anatomical locations, and sizes of pilomatricoma among different pediatric patients. However, few studies have comprehensively summarized the correlations between these features and treatment efficacy, which is crucial for enhancing clinicians' understanding and diagnostic accuracy of the disease.

Fatigue-free ferroelectricity in HfZrO ultrathin films via interfacial design.

Due to traits of CMOS compatibility and scalability, HfO-based ferroelectric ultrathin films are promising candidates for next-generation low-power memory devices. However, their commercialization has been hindered by reliability issues, with fatigue failure being a major impediment. Here, we report superior ferroelectric performances with fatigue-free behavior in interface-designed HfZrO-based ultrathin heterostructures.

Environmental fate of POPs under climate change: Regulation of POPs distribution by soil organic matter and microbial communities during glacial retreat.

Glacial retreat zones are highly sensitive to climate change. This makes them unique models of vegetation succession that reflect interactions among soil development, microbes, and the behavior of chemicals. In this study, the fate of persistent organic pollutants (POPs) during soil succession in glacial retreat areas was investigated, with a focus on the roles of soil organic matter (SOM) and microbial communities.

Unexpected Generation of Singlet Oxygen at the Air-Water Interface of Aqueous Microdroplets.

Singlet oxygen (O) is a classical nonradical reactive oxygen species (ROS), which is of great significance in both natural and engineering environments. Here, we found that O can be generated spontaneously at the air-water interface of microdroplets under dark conditions. O isotope labeled O (O) and water (HO) were used to identify the source and formation pathway of O and previously reported ROS produced in microdroplets.

SPARC promotes malignant progression and predicts poor prognosis in cervical squamous cell carcinoma.

Background: Cervical cancer continues to pose a significant global threat to women's health, with rising incidence and mortality rates in recent years. Pathologically, cervical cancer primarily comprises squamous cell carcinoma (CESC) and adenocarcinoma, where CESC represents more than 80 % of diagnosed cases. Most patients present with advanced-stage CESC at diagnosis, and nearly half eventually develop distant metastases, leading to markedly diminished cure rates and five-year survival below 30 %.

TSIX: A Novel Long Noncoding RNA in Cancer Development and Progression.

Long noncoding RNAs (LncRNAs) represent a group of long noncoding RNA molecules exceeding 200 nt in length and lacking the capacity for protein coding. In recent years, the function and progress of lncRNA have attracted the attention of more and more researchers. Specifically, the long non-coding RNA, TSIX (TSIX transcript, XIST antisense RNA), plays a significant role in ensuring random X-chromosome inactivation.

Research on Bearing Fault Diagnosis Method for Varying Operating Conditions Based on Spatiotemporal Feature Fusion.

In real-world scenarios, the rotational speed of bearings is variable. Due to changes in operating conditions, the feature distribution of bearing vibration data becomes inconsistent, which leads to the inability to directly apply the training model built under one operating condition (source domain) to another condition (target domain). Furthermore, the lack of sufficient labeled data in the target domain further complicates fault diagnosis under varying operating conditions.

Therapeutic potential of isoproterenol in androgenetic alopecia: activation of hair follicle stem cells via the PI3K/AKT/β-Catenin signaling pathway.

Background: Androgenetic alopecia (AGA) is characterized by the depletion or dormancy of hair follicle stem cells (HFSCs), leading to hair thinning and miniaturization. Reactivating the dormant HFSCs is a promising therapeutic approach. Adrenergic β2 receptor (ADRB2) activation has been shown to promote hair growth in animal models via the Sonic Hedgehog (SHH) pathway, but its potential for treating clinical AGA patients remains unexamined.

Dynamic characteristics analysis and realization of a high-repetition, high-energy, and high-power thin disk regenerative amplifier.

This paper presents a comprehensive theoretical and numerical analysis of a thin disk regenerative amplifier (RA) based on the Frantz-Nodvik equations. By analyzing key dynamic parameters, such as seed energy, pump power, repetition frequency, and round trips, the study provides a method to obtain the optimal operational parameters for maximizing both pulse energy and conversion efficiency. A highly stable, double-pass thin disk regenerative amplifier with a Fourier transform resonator was developed.

Provincial export cost implications of the EU Carbon Border Adjustment Mechanism on China's metal industry.

China's exports of iron and steel, aluminum, and their products (ISAP) are significantly affected by the European Union (EU) Carbon Border Adjustment Mechanism (CBAM). This study introduces a modeling framework coupling a global multi-regional input-output table and the integrated assessment model GCAM-China, both of which embed Chinese provinces, to dynamically assess provincial cost implications of CBAM for China's ISAP exports, considering China's upcoming 2035 Nationally Determined Contributions. By 2034, direct emissions from China's ISAP exports to the EU reach 5.

Identification and assessment of microplastics in wastewater treatment plants using laser direct infrared spectroscopy and depolymerization-coupled liquid chromatography-tandem mass spectrometry.

The pollution levels and distribution characteristics of microplastics (MPs) in wastewater treatment plants (WWTPs) need to be well explored and evaluated for better control of MPs. The use of a single method may be inadequate to achieve comprehensive information on MPs. Studies using combinations of different analytical methods are rare and challenging.

Ergothioneine regulates the paracrine of dermal papilla cells through SIRT1/Nrf2 pathway to antagonize oxidative stress and natural hair follicle aging.

Hair graying and loss are significant indicators of aging, and oxidative stress plays an essential role in this progression. Ergothioneine (EGT), potent antioxidant from edible fungi and certain prokaryotes, regulates the antioxidant defense system. However, its anti-aging effects on hair follicles (HFs) remain obscure.

Research on Open-Set Recognition Methods for Rolling Bearing Fault Diagnosis.

In rolling bearing fault diagnosis, when an unknown fault is present, the Closed-Set Recognition (CSR) method tends to misclassify it as a known fault. To address this issue, an Open-Set Recognition (OSR) framework is proposed for rolling bearing fault diagnosis in this study. The framework is built upon a serial multi-scale convolutional prototype learning (SMCPL) network, enhanced with an efficient channel attention (ECA) mechanism to extract the most critical fault features.

Quantification of five intracellular and extracellular methionine pathway intermediates using stable isotope dilution UHPLC-MS/MS.

Unraveling the intricate mechanisms underlying methionine metabolism reprogramming and its extracellular release during apoptosis requires robust and sensitive quantification of key intermediates in both intracellular and extracellular compartments. Here, we developed a highly sensitive and precise stable isotope-dilution UHPLC-MS/MS method for simultaneous quantification of five critical intermediates in methionine cycle and methionine salvage pathway: l-methionine (Met), S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH), l-homocysteine (Hcy), and 5'-methylthioadenosine (5'-MTA). Through optimizing mobile phase additives and implementing an effective dilution strategy, this method minimizes matrix effects.

[Volatile Organic Compounds Emission Characters Based on Business Operations of the Vehicle Repairing Industry in Beijing].

The vehicle repair industry is an important source of volatile organic compound （VOCs） emissions in Beijing. Currently, research on VOCs emissions from the auto repair industry focuses only on organized emissions from spray painting booths and research has not been carried out on the emission characteristics of VOCs in sheet metal, machine repair, and beauty processes. A comprehensive list of VOCs emissions from the entire production process of the auto repair industry lacks.

Quantification of Epigenetic DNA and RNA Modifications by UHPLC-MS/MS Technologies: New Concepts and New Improvements for the Special Collections.

Dynamic and reversible DNA and RNA modifications are essential for cell differentiation and development. Aberrant epigenetic modifications are closely associated with the occurrence and progression of diseases, serving as potential markers for cancer diagnosis and prognosis. Ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) offers distinct advantages in the qualitative and quantitative analysis of various modifications due to its sensitivity, specificity, and accuracy.

The TNFRSF1B Connection: Implications for Androgenetic Alopecia Pathogenesis and Treatment.

Androgenetic alopecia (AGA) is a prevalent form of hair loss with poorly understood mechanisms. This study investigates the association between the tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) gene and AGA, aiming to validate its potential causal relationship. Transcriptomic data from AGA patients were obtained from the Gene Expression Omnibus (GEO) database to identify differentially expressed genes (DEGs).

Enhanced visible-light photocatalytic degradation of antibiotics using aminoguanidine-functionalized iron-based metal-organic frameworks.

The degradation of antibiotics in wastewater has gained significant attention due to the detection of high concentrations of these contaminants in water systems. Moreover, the complex composition of water matrices, which contain various coexisting components, poses significant challenges to their effective removal. To address this, in the present study, we investigated the selective photocatalytic degradation of antibiotics using metal-organic frameworks (MOFs).

Premade Incisions vs. Stick and Place: A Multicenter Retrospective Comparison of Hair Transplantation Outcomes in China.

Background: Based on the differences in the procedures of recipient site creation and graft placement, hair transplantation implantation techniques can be classified into premade incisions (PI) and stick and place (S&P). However, limited studies have systematically compared their clinical outcomes.

Methods: A retrospective analysis was conducted on 191 Chinese patients (95 in the PI group and 96 in the S&P group) from four hospitals, undergoing follicular unit excision between August 2022 and August 2024.

An Adaptive Optimization Method for Acoustic Temperature Measurement Topology Based on Multiple Sub-Objectives.

Recent years have seen a surge in study interest in acoustic temperature measurement because of its exceptional non-invasiveness, high precision, and fast response characteristics. Its main benefit is that it may rely on the temperature field reconstruction technique to obtain the entire temperature distribution information, circumventing the limitations of point-type thermometry. Studies have shown that the acoustic wave transducer topology is a key factor affecting the reconstruction effect.

Total saponins from Panax japonicus alleviate insulin resistance via exosomal miR204/Elovl6-mediated adipocyte-macrophage crosstalk.

Background: Insulin resistance (IR) is a key factor in the development of type 2 diabetes, and M2-like macrophages are important in maintaining normal glucose homeostasis. Our previous research has demonstrated that the total saponins from Panax japonicus (TSPJ) reduce IR in adipocytes and promote the M2 polarization of macrophages, but the molecular mechanism is unclear.

Purpose: In the study, we aimed to elucidate whether TSPJ mitigate IR by enhancing the intercellular communication between adipocytes and macrophages and describe how the exosomes from bone marrow-derived macrophages (BMDMs) modulate the insulin sensitivity of adipocytes via miR204.

Direct Fluorescence Anisotropy Detection of miRNA Based on Duplex-Specific Nuclease Signal Amplification.

The dysregulation of microRNAs (miRNAs) is associated with various diseases, including cancer, so miRNAs are considered a potential biomarker candidate for disease diagnosis and therapy. However, the direct, rapid, sensitive, and specific detection of miRNAs remains quite challenging due to their short length, sequence homology, and low abundance. Herein, we propose a simple and homogeneous fluorescence anisotropy (FA) strategy for the direct and rapid (∼35 min) quantification of miRNA-21 based on duplex-specific nuclease (DSN)-assisted signal amplification.

An updated meta-analysis of the efficacy and safety of robot-assisted laparoscopy hepatectomy and laparoscopic hepatectomy in the treatment of liver tumors.

Background: To compare the efficacy and safety of robot-assisted laparoscopic hepatectomy (RALH) with laparoscopic hepatectomy (LH) in the treatment of liver tumors.

Methods: A comprehensive search of English-language literature was conducted in PubMed, Embase, Web of Science, and the Cochrane Library from January 2000 to June 2024. Studies comparing RALH and LH for liver tumors were identified, and after qualitative evaluation, a meta-analysis was performed using Stata 16.

Spherical Nucleic Acids-Directed Cryosynthesis of Manganese Nanoagents for Tumor Imaging and Therapy.

DNAzyme-based theranostic nanotechnologies that can respond to specific tumor pathophysiological parameters hold great promise for tumor diagnostics and effective treatments. However, their clinical translation is hindered by insufficient intracellular availability of essential metal cofactors required for DNAzyme activation. To overcome this limitation, we developed a temperature-controlled synthesis strategy for fabricating multifunctional DNA-templated manganese carbonate nanoparticles (DtMnP).