CLDN4 promotes ferroptosis and inflammation involving JAK2/STAT3 pathway in acute pancreatitis.

Acute pancreatitis (AP) is a severe inflammatory disease characterized by pancreatic acinar cell injury and oxidative stress. Ferroptosis, an iron-dependent form of cell death driven by lipid peroxidation, has been implicated in AP pathogenesis. However, the molecular mechanisms linking ferroptosis to AP remain unclear.

Superpath is Superior to Posterolateral Approach for Hemiarthroplasty in Geriatric Patients with High Risk for Dislocation.

Background: Dislocation following hemiarthroplasty (HA) for femoral neck fractures in the elderly was a rare but severe complication. Tissue-sparing SuperPATH approach for HA had been described with promising results in terms of function recovery, transfusion and dislocation rate. The aim of the present study was to investigate the clinical outcomes, perioperative complications, and mortality rate through SuperPATH (SP) and posterolateral (PL) approach in geriatric patients with high dislocation risk.

Quercetin alleviates acute pancreatitis by modulating glycolysis and mitochondrial function via PFKFB3 inhibition.

Objective: Acute pancreatitis (AP) is a severe inflammatory disease associated with dysregulated glycolysis and mitochondrial dysfunction. This study investigates the therapeutic potential of quercetin, a novel PFKFB3 inhibitor, in modulating glycolysis and mitochondrial function to alleviate AP.

Methods: We conducted homology analysis of the PFKFB3 protein and identified quercetin as a potential inhibitor through molecular docking.

Decreased plasma APOA1 levels are associated with increased severity of placenta accreta spectrum disorders: a nested case-control study.

Objective: Placenta accreta spectrum (PAS) disorders are a series of gestational diseases, with severe adverse outcomes. Apolipoprotein A1 (APOA1) is a lipid molecule that plays a role in cell invasion, inflammation and immune response. This study aimed to elucidate the relationship between APOA1 and PAS, as well as its adverse outcomes.

One-pot, uracil-DNA-glycosylase-aided, and multi-indicator nanobiosensor detection platform for identifying Brucella melitensis from the Brucella spp.

Background: Brucellosis, a zoonotic chronic contagious disease caused by spp., is primarily associated with species such as () and , among which is recognized as the most virulent in causing human brucellosis.

Methods: Herein, we report a newly “one-pot” detection protocol for the identification of from the genus using the multiplex multiple cross-displacement amplification (MCDA) combined with dUTP-assisted uracil-DNA-glycosylase (UDGase) and gold nanoparticles lateral flow assay (AuNPs-LFA) biosensor (termed mMCUDA).

Establishment of a CRISPR/Cas12b-Based Multiple Cross Displacement Amplification Assay for the Rapid, Sensitive, and Specific Detection of .

(), a major pathogenic species within the genus, causes ovine epididymitis. Although the isolation and identification of remain the gold standard for diagnosis, these methods are unsuitable for early detection. The traditional polymerase chain reaction (PCR) offers faster detection but requires specialized equipment such as PCR thermal cyclers and gel electrophoresis imagers, limiting its use in basic laboratories.

technology and ADMET research in traditional Chinese medicine.

Traditional Chinese medicine (TCM) faces many challenges in the study of absorption, distribution, metabolism, excretion and toxicity (ADME/T) because of its complexity and diversity. models, which can effectively simulate the environment and provide a platform for the analysis of the efficacy and toxicity mechanism of TCMs, play important roles in the study of TCM compounds. This paper evaluates the advantages and limitations of models (including cell models, noncell traditional models, OoC and organoid technology) from the perspective of ADMET.

Unlocking the Treatment Code for Uveitis: The Potential Role of Epigenetics.

Purpose: To systematically explore the multi-dimensional mechanisms of the epigenetic regulatory network in uveitis, address the challenges faced by traditional treatments, and provide new ideas and directions for the precise diagnosis and treatment of this disease.

Methods: A systematic review was conducted on the epigenetic regulation in uveitis, focusing on analyzing abnormal DNA methylation, dynamic balance of histone modifications, disease-specific regulatory characteristics of non-coding RNAs, as well as their applications in diagnosis, prognosis assessment, and targeted treatment strategies.

Results: The epigenetic regulatory network in uveitis includes abnormal DNA methylation driving immune imbalance, histone modification dynamics regulating inflammatory signal amplification, and disease-specific non-coding RNAs.

Targeting PFKFB3 to restore glucose metabolism in acute pancreatitis via nanovesicle delivery.

Background: Acute pancreatitis (AP) is a severe inflammatory disease frequently accompanied by disturbances in glucose metabolism, which further complicate the disease prognosis. This study aims to explore the role of PFKFB3, a key glycolytic enzyme, in regulating glucose metabolism in AP and assess the potential of PFKFB3 inhibition via nanovesicle delivery to mitigate metabolic dysfunction.

Methods: Transcriptomic data from Gene Expression Omnibus (GEO), including single-cell RNA sequencing (scRNA-seq) and bulk RNA sequencing, were analyzed to investigate the molecular mechanisms involved in glucose metabolism dysregulation in AP.

Clinical and radiographic evaluation of Ponseti method for neonate congenital clubfoot.

Objective: The objective of this study was to evaluate the clinical and radiographic outcomes of children with congenital clubfoot following treatment with the Ponseti method.

Methods: We conducted a retrospective analysis of radiographic data from 20 children (12 males and 8 females) aged between 2 years 6 months and 7 years who underwent Ponseti method treatment for congenital clubfoot. The beginning treatment age was below 4 weeks after birth.

Naa10p impairs PGC-1α/Pparγ2 interaction to inhibit mitochondrial protection in pancreatitis.

Naa10p disrupts the protective mitochondrial UCP1 pathway in acute pancreatitis (AP). This study demonstrates that Naa10p upregulation in AP correlates with decreased UCP1 expression and increased reactive oxygen species production. Silencing Naa10p improved cell survival, suppressed inflammation, and enhanced UCP1 levels by promoting PGC-1α/Pparγ2 interaction.

Harnessing natural products for myocardial infarction therapy: Mechanistic insights and translational opportunities.

Myocardial infarction (MI) continues to pose significant challenges in clinical treatment despite medical advancements. Natural products (NPs) derived from botanical and biological sources demonstrate considerable therapeutic potential through their ability to simultaneously address multiple pathological features of MI. This comprehensive review examines the past decade of research investigating the therapeutic effects of various classes of NPs, notably terpenoids, flavonoids, alkaloids, phenolic acids, and phenylpropanoids in MI treatment.

Investigation into the Mechanisms of Paeoniae Radix Rubra in the Treatment of Venous Thrombosis Using Network Pharmacology, Bioinformatics, and Molecular Docking Techniques.

Objective: This study investigates the potential targets and mechanisms of Paeoniae Radix Rubra (PRR) in treating Venous Thrombosis (VTE) by employing network pharmacology, bioinformatics analysis, and molecular docking validation.

Methods: Active components of PRR were identified via TCMSP. VTE-related genes were screened from GEO datasets, and WGCNA analyzed key modules.

Morphological, lytic, and genetic characteristics of three phages isolated from Inner Mongolia Autonomous Region.

This study comprehensively examined three phages (A1, NMY-1, and NMY-2) isolated from Inner Mongolia Autonomous Region. Electron microscopy classified them as short-tailed phages. A1 and NMY-1 lysed smooth strains of , , and , while NMY-2 lysed rough strains of and .

Identification of a Biomarker Panel in Extracellular Vesicles Derived From Non-Small Cell Lung Cancer (NSCLC) Through Proteomic Analysis and Machine Learning.

Antigen fingerprint profiling of tumour-derived extracellular vesicles (TDEVs) in the body fluids is a promising strategy for identifying tumour biomarkers. In this study, proteomic and immunological assays reveal significantly higher CD155 levels in plasma extracellular vesicles (EVs) from patients with non-small cell lung cancer (NSCLC) than from healthy individuals. Utilizing CD155 as a bait protein on the EV membrane, CD155+ TDEVs are enriched from NSCLC patient plasma EVs.

Tick-borne agents in the fowl tick Argas persicus from northwest and northeast China.

Although tick-borne agents have been extensively studied, etiological investigations on soft ticks are still relatively rare. In this study, we collected 114 Argas persicus ticks from two provinces (Xinjiang and Heilongjiang) located in northwest and northeast China, respectively, and screened them for tick-borne agents. Two Rickettsia species were identified in A.

Safety and efficacy of migalastat in adolescent patients with Fabry disease: Results from ASPIRE, a phase 3b, open-label, single-arm, 12-month clinical trial, and its open-label extension.

Fabry disease (FD) is a progressive, multisystemic, X-linked lysosomal disorder caused by reduced or absent α-galactosidase A activity due to galactosidase alpha (GLA) gene variants. Although clinical manifestations of FD often appear in childhood, approved treatments for the management of FD in children and adolescents are limited. ASPIRE (NCT03500094) was a phase 3b, two-stage, open-label, multicenter study evaluating the safety, pharmacokinetics, and efficacy of migalastat in adolescents 12 to <18 years, ≥ 45 kg with FD and amenable GLA variants.

Ultrasensitive and highly specific detection of the genus and . by CRISPR/Cas12b-multiple cross displacement amplification technique.

Brucellosis is caused by members of the spp. and remains one of the world's major zoonotic diseases. () as the most contagious species cannot be ignored as an essential source of infection for brucellosis, especially in countries/regions dominated by animal husbandry.

A CRISPR/cas13a-assisted precise and portable test for nucleic acid detection.

Introduction: infection in humans or animals can lead to brucellosis, which has the potential to significantly impact both the economy and public health. Currently, molecular biological methods for diagnosing brucellosis are either complex or have low sensitivity, and it is difficult to apply them in real-life settings in the field. Therefore, this study aims to establish a rapid and convenient nucleic acid-based molecular biology method for on-site rapid detection of and early clinical screening of brucellosis.

A family cluster of infections possibly due to contact with a sika deer in Northeast China.

Brucellosis is one of the most common zoonotic diseases worldwide. Although deer are reportedly infected by spp., deer-associated brucellosis cases have been rare.

Toward Cyborg: Exploring Long-Term Clinical Outcomes of a Multi-Degree-of-Freedom Myoelectric Prosthetic Hand.

Recent advancements in robotics and sensor technology have facilitated the development of myoelectric prosthetic hands (MPHs) featuring multiple degrees of freedom and heightened functionality, but their practical application has been limited. In response to this situation, formulating a control theory ensuring the hand dexterity of highly functional MPHs has garnered marked attention. Progress in this field has been directed toward employing machine-learning algorithms to process electromyogram patterns, enabling a broad spectrum of hand movements.

Oxidative stress-mediated abnormal polarization of decidual macrophages promotes the occurrence of atonic postpartum hemorrhage.

Postpartum hemorrhage (PPH) is the leading cause of maternal mortality worldwide. However, the mechanism underlying atonic PPH remains partially elucidated. Multi-omics revealed that differentially expressed proteins and metabolites were enriched in the immune-inflammation pathway in the vaginal blood of patients with atonic PPH.

A review of L.: a valuable plant with profound biological significance.

L. (PCL) is an annual herb of the genus Psoralea in the family Fabaceae, and its mature fruit can be used medicinally as a precious medicinal herb to tonify muscles and bones. With the deepening of research, its applications to various industries, including food, agriculture, and cosmetics, with products being developed in countries such as Vietnam, India, and Japan.