Percutaneous nephrolithotomy under total X-ray free technique in horseshoe kidney stones: a 10-year experience from a large stone center.

Objectives: To report outcomes of complete ultrasound-guided percutaneous nephrolithotomy (PCNL) for horseshoe kidney (HSK) stones at a high-volume center and evaluate a novel technique (Needle-perc-assisted endoscopic surgery, NAES) for these patients.

Patients And Methods: We retrospectively reviewed all HSK stone patients who underwent PCNL at our institution over a 10-year period. The NAES technique was utilized during the most recent 4 years.

Effects of Gender Differences in 677C > T and Homocysteine Level on the Occurrence of Adverse Pregnancy Outcomes.

The MTHFR 677C > T polymorphism in women has been associated with an increased risk of deep venous thrombosis and adverse pregnancy outcomes (APOs). However, research concerning its effects in men remains limited. This study examined 662 adults with a history of pregnancies affected by chromosomal abnormalities (CAs: 343 females and 319 males), 137 adults with a history of pregnancies affected by cleft lip and palate (CLP: 71 females and 66 males), and 133 adults with a history of biochemical pregnancies (BPs: 65 females and 68 males), forming three case groups.

Circadian disruption and ROS-NLRP3 signaling mediate sleep deprivation-enhanced silica nanoparticle toxicity in lacrimal glands.

Sleep deprivation (SD) and exposure to engineered nanomaterials such as silica nanoparticles (SiNPs) are emerging risk factors for ocular surface disorders, particularly dry eye disease. However, the molecular mechanisms underlying their combined impact on lacrimal gland function remain unclear. In this study, we investigated the synergistic effects of SD and SiNPs exposure on circadian regulation, oxidative stress, inflammation, and structural integrity of the extraorbital lacrimal glands (ELGs) in C57BL/6J mice.

Regulation of Ghrelin Production and Food Intake by Gastric Adenylyl Cyclase Type 8.

Ghrelin is a peptide hormone primarily produced by ghrelin cells in the stomach, playing a vital role in the regulation of eating behavior. Adenyl cyclase 8 (ADCY8), a key downstream signaling factor of G protein-coupled receptors, is essential for maintaining energy homeostasis by modulating levels of cyclic adenosine monophosphate (cAMP). Nevertheless, how ADCY8 modulates ghrelin levels and affects food intake is not well understood.

Effect of high-flow nasal oxygen therapy on perioperative hypoxemia in children: a systematic review and meta-analysis.

Background: Hypoxia is the leading cause of complications and death during anesthesia. The risk of hypoxemia in children is higher than in adults. The use of high-flow nasal cannula (HFNC) can prevent desaturation of blood oxygen saturation in children.

Running exercise reverses depression-induced bone marrow myeloid skewing and neuroinflammation via the lactate-GPR81 axis.

Exercise has emerged as a powerful non-pharmacological intervention for mental health, yet its precise mechanisms in alleviating depression remain incompletely understood. Here, using a chronic unpredictable stress (CUS) mouse model, we demonstrate that prolonged stress induces hematopoietic stem cell (HSC) activation, leading to myeloid-biased hematopoiesis and an overproduction of Ly6C monocytes, which contribute to systemic and hippocampal inflammation. Notably, treadmill running exercise effectively mitigated depressive and anxiety-like behaviors in CUS mice by suppressing stress-induced HSC activation, restoring hematopoietic balance, and reducing peripheral Ly6C monocyte infiltration into the brain.

The causal relationship between inflammatory cytokines and lung cancer: a two-sample bidirectional mendelian randomization study.

Objective: The objective is to investigate the causal link between inflammatory cytokines and lung cancer through the application of a two-sample bidirectional Mendelian randomization (MR) approach.

Method: This study gathered data from genome-wide association studies (GWAS), focusing on 91 inflammatory cytokines associated with lung cancer. The investigation into the causal link between inflammatory cytokines and lung cancer was performed utilizing several methods, including the inverse variance weighted (IVW) approach, weighted median (WM) method, MR Egger regression, weighted mode, and simple mode.

Novel PNLDC1 mutations underlie nonobstructive azoospermia in humans and mice.

PIWI-interacting RNAs (piRNAs) are small regulatory RNAs (21-35 nucleotides) exclusively expressed in germ cells, where they play a critical role in transposable element repression and post-meiotic gene regulation. The poly(A)-specific RNase-like domain-containing 1 (PNLDC1) protein is essential for piRNA maturation, specifically in 3'-end trimming. Disruption of PNLDC1 has been implicated in nonobstructive azoospermia (NOA) and male infertility.

Analysis of subjective efficacy of 5-aminolevulinic acid photodynamic therapy in the treatment of refractory vulvar lichen sclerosus.

Objective: This study evaluated the therapeutic effect of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) in refractory Vulvar lichen sclerosus (VLS) patients.

Methods: This prospective study enrolled a total of 60 patients with refractory VLS. These patients received 6 sessions of ALA-PDT treatment.

AAV-mediated STC-1 expression mitigates neuroinflammation and preserves visual function in degenerative retinopathy.

Background: Retinal degeneration (RD) is a chronic retinopathy that characterized by the progressive photoreceptor apoptosis and visual impairments. However, due to the complicated pathogenesis of RD, there is no effective treatment at present. This study intends to verify the therapeutic effect and mechanism of stanniocalcin-1 (STC-1) in the sodium iodate (NaIO)-induced RD mouse model.

Stapokibart for Severe Uncontrolled Chronic Rhinosinusitis With Nasal Polyps: The CROWNS-2 Randomized Clinical Trial.

Importance: Chronic rhinosinusitis with nasal polyps causes severe symptoms and impaired quality of life. Stapokibart is a novel monoclonal antibody that targets interleukin 4Rα.

Objective: To assess the efficacy and safety of stapokibart as an add-on treatment to intranasal corticosteroids in patients with severe uncontrolled chronic rhinosinusitis with nasal polyps.

[Construction of a machine learning prognostic prediction model based on psoas muscle index for patients with decompensated liver cirrhosis].

To explore the effect of psoas muscle index (PMI) and construct a machine learning model to validate the 180-day prognosis in patients with decompensated liver cirrhosis. Retrospective data were collected from patients with decompensated liver cirrhosis at Henan Provincial People's Hospital from January 2022 to November 2022. The area of the psoas muscle index (PMI) at the level of the third lumbar vertebra was measured and calculated based on the abdominal X-ray computed tomography images stored in the Eastern China Hospital Information System (HIS).

as a biomarker influences the prognosis and immune microenvironment of low-grade gliomas.

Background: In recent years, FAM111B has been found to be closely related to the occurrence and development of various cancers. However, the role of FAM111B in low-grade glioma (LGG) remains unclear.

Objective: This study aims to investigate the regulatory role of FAM111B in the progression of LGG.

Fibro-NPC: a pathogenic subtype identified at single-cell resolution with secreted SFRP4 as a biomarker in intervertebral disc degeneration.

Background: Intervertebral disc degeneration (IDD) is a primary cause of low back pain and, in severe cases, can lead to disability. Current treatments for low back pain remain limited in efficacy, underscoring the need for a deeper understanding of the molecular mechanisms driving IDD. The degeneration process is primarily driven by an imbalance in the extracellular matrix, largely due to the senescence of nucleus pulposus cells (NPCs).

The association between glycemic indicators and bone mineral density and osteoporosis: a cross-sectional study.

To investigate the relationship between glycemic indicators (HbA1c and FPG) and bone mineral density (BMD) as well as osteoporosis in adults. A total of 1445 participants from the "The longitudinal investigation of osteoarthritis and cardiovascular health status" cohort were recruited and classified into normal BMD, osteopenia, and osteoporosis groups based on BMD. Data on sociodemographic factors, anthropometric measurements, medical history, and FPG samples were collected.

Circadian rhythms in ophthalmic diseases.

Circadian rhythms, governed by the suprachiasmatic nucleus of the hypothalamus, orchestrate a wide range of physiological processes, including those implicated in ophthalmic diseases. These biological rhythms influence both normal ocular function and disease pathogenesis, with many ophthalmic conditions exhibiting altered circadian rhythmicity in frequency or amplitude. Disruptions to circadian homeostasis may exacerbate disease symptoms and negatively affect treatment outcomes, ultimately impairing patients' quality of life.

Transcriptomic profiling reveals crizotinib-induced hepatotoxicity through ROS-mediated activation of the JNK/NLRP3 pathway.

Crizotinib, a first-generation tyrosine kinase inhibitor, demonstrates excellent clinical efficacy in treating non-small cell lung cancer (NSCLC). However, its clinical application is often limited by severe hepatotoxicity, the underlying mechanisms of which remain poorly understood. This study aimed to investigate the molecular mechanisms of crizotinib-induced hepatotoxicity in mice using transcriptomic analysis.

Causal association between immunoglobulin G N-glycosylation patterns and diabetic complications: two-sample mendelian randomization.

Background: To elucidate the correlation between immunoglobulin G (IgG) N-glycosylation patterns (IGPs) and diabetes complications, including diabetic nephropathy (DN), diabetic retinopathy (DR) and diabetic maculopathy (DM). We performed a two-sample Mendelian randomization (MR) study.

Methods: We obtained genome-wide association studies (GWAS) summary statistics related to 77 IGPs and diabetic complications from the MRC Human Genetics Unit and the FinnGen consortium.

Perioperative safety evaluation of intersphincteric resection combined with rectal eversion and total extra-abdominal resection: a prospective randomized controlled trial.

Background: Colorectal cancer remains a significant global health burden, with low rectal tumors posing unique surgical challenges due to their proximity to the anal verge. Traditional abdominoperineal resection (APR) compromises quality of life with permanent colostomy, while sphincter-preserving techniques like laparoscopic intersphincteric resection (L-ISR) face technical limitations. This study evaluates the safety and efficacy of a novel technique - ISR combined with rectal eversion and total extra-abdominal resection (ISRER) - aimed at reducing anastomotic complications, and enhancing anal preservation in anatomically challenging patients.

FN1 from cancer-associated fibroblasts orchestrates pancreatic cancer metastasis via integrin-PI3K/AKT signaling.

Objective: The metastasis of pancreatic ductal adenocarcinoma (PDAC) is closely linked to the remodeling of cancer-associated fibroblasts (CAFs) within the tumor microenvironment (TME), though the precise molecular mechanisms remain unclear. This study aims to elucidate the role of CAFs in PDAC metastasis.

Methods: We integrated transcriptomic (GSE183795) and single-cell sequencing data (GSE156405) to screen for core genes associated with PDAC.

A study of HPV self-sampling by female residents in communities of Zhengzhou, Henan Province, China: a cross-sectional observational study.

Objective: Evaluate the feasibility of HPV self-sampling typing and quantitative detection as a cervical cancer screening scheme, and provide new methods to reduce the incidence of cervical cancer.

Methods: This was a cross-sectional observational study of 1,228 female residents in communities in Zhengzhou city, Henan Province, who participated in HPV self-sampling detection. All the samples were subjected to HPV typing and quantitative detection.

The rising burden of Alzheimer's and other dementias: role of high fasting plasma glucose from 1990 to 2021.

Background: Dementia, one of the top 10 causes of death globally, imposes significant health and socioeconomic/socioeconomic burdens, with prevalence projected to reach 82 million by 2030. High fasting plasma glucose (HFPG) is a prominent modifiable risk factor for dementia in 2021. This study aims first to examine the global trend in dementia burden and the disability-adjusted life years and death attributable to HFPG from 1990 to 2021 and second to define age-specific disparities in dementia burden among older populations.

Global, regional, and national burden of sudden infant death syndrome, 1990-2021: a comprehensive analysis of GBD 2021 data with insights into the impact during the COVID-19 pandemic.

Background: Sudden infant death syndrome (SIDS) remains a leading cause of infant mortality globally. Although the global burden has generally declined over recent decades, the COVID-19 pandemic may have influenced these trends. This study investigates whether the global SIDS burden has changed, particularly during the COVID-19 pandemic.

The safety and effectiveness of sintilimab versus camrelizumab, both plus targeted drugs, in advanced hepatocellular carcinoma.

Objectives: We aimed to evaluate the effectiveness and safety of sintilimab versus camrelizumab, both plus targeted drugs, for advanced hepatocellular carcinoma (HCC) in the real world. Then the effectiveness was compared between sintilimab-lenvatinib and camrelizumab-apatinib.

Methods: Patients diagnosed as advanced HCC were included from January 2017 to December 2023.

Reliability, validity, and acceptability of the simplified Mandarin Chinese EORTC QLQ-OPT30 for uveal melanoma patients.

This study aimed to translate the EORTC QLQ-OPT30 into Mandarin (Simplified) Chinese and assess its reliability, validity, and acceptability among Chinese uveal melanoma (UM) patients. From January 2023 to June 2024, 60 UM patients from a tertiary hospital in central China were enrolled. The process involved both forward and back translation to develop the Mandarin (Simplified) Chinese version of the EORTC QLQ-OPT30.