Preliminary exploration of prediction model and nomograph drawing of Doppler peak systolic velocity ratio, peak systolic velocity 1 and peak systolic velocity 2 of ophthalmic artery in pregnant women for small for gestational age infants.

Objective: To explore the predictive value of peak systolic velocity (PSV) ratio, PSV1 and PSV2 of ophthalmic artery Doppler in pregnant women for small for gestational age (SGA) infants and to construct a nomogram prediction model.

Methods: A total of 201 pregnant women who visited our hospital from March 2022 to June 2024 were selected as the research subjects, and their clinical data and ophthalmic artery Doppler parameters were collected. The data were randomly divided into a training set ( = 295) and a verification set ( = 126) in a 7:3 ratio.

Airway microbiota and immunity associated with chronic obstructive pulmonary disease severity.

Background: Chronic Obstructive Pulmonary Disease (COPD) is characterized by progressive airflow limitation and chronic inflammation. Although airway microbes and host immunity are known contributors, the molecular mechanisms underlying disease severity remain unclear. This study explores microbial dysbiosis and host immune responses across varying COPD severities.

ITK overexpression enhances T cell cytotoxicity against DLBCL through the TCR-Ca-Calcineurin-NFAT-IFN-γ pathway.

Diffuse Large B-Cell Lymphoma (DLBCL) is the most prevalent pathological subtype of non-Hodgkin lymphoma (NHL). Patients with DLBCL often experience extremely poor prognoses due to drug resistance or relapse, and the limitations of existing treatment regimens are evident. This study focuses on the mechanistic role and clinical significance of interleukin-2-inducible T-cell kinase (ITK) in DLBCL.

Effects of endoscopic minimally invasive surgery on olfactory function and quality of life in patients with chronic rhinosinusitis and nasal polyps.

Objective: To evaluate the efficacy of endoscopic minimally invasive surgery (EMIS) in improving olfactory function and quality of life among patients with chronic rhinosinusitis with nasal polyps (CRSwNP).

Methods: A cohort of 117 patients diagnosed with CRSwNP between January 2020 and June 2024 were included in this retrospective study. Patients were assigned to either a control group (n=50, treated with conventional endoscopic sinus surgery [ESS]) or a research group (n=67, treated with EMIS).

Analysis of microorganisms and drug-resistance mutations detected by probe-capture metagenomics among HIV-infected patients with pneumonia.

Background: Probe-Capture Metagenomics is a newly developed method for detecting infectious pathogens. However, its application in HIV-infected patients with pulmonary infection remains limited. This study utilized Probe-Capture Metagenomics to analyze lung microbiomes and Drug Resistance Mutations of HIV and bacteria in people living with HIV (PLWH) with pneumonia.

Intrapersonal strengths and interpersonal support: predicting academic buoyancy through psychological capital and growth mindset.

Introduction: This mixed-methods study, situated within the framework of personality and social psychology, examines the interplay of psychological capital, social support, and growth mindset in predicting academic buoyancy among undergraduate English majors across three universities in mainland China.

Methods: In the quantitative phase, established instruments were utilized to assess direct and mediated relationships among 516 undergraduate English majors, analyzed via structural equation modeling. The qualitative phase involved semi-structured interviews with 18 students to offer richer insights into these dynamics, analyzed using thematic analysis.

AHCTF1 Functions as an Oncogenic Factor and Promotes Tumor Progression in Hepatocellular Carcinoma.

Background: Hepatocellular carcinoma (HCC) is a leading cause of cancer-related mortality, with poor prognosis due to late diagnoses and limited therapeutic targets. The AT-hook containing transcription factor 1 (AHCTF1) is linked to various cancers, yet its role in HCC is unclear.

Aims: This study aimed to investigate AHCTF1's role in HCC progression.

FN1 from cancer-associated fibroblasts orchestrates pancreatic cancer metastasis via integrin-PI3K/AKT signaling.

Objective: The metastasis of pancreatic ductal adenocarcinoma (PDAC) is closely linked to the remodeling of cancer-associated fibroblasts (CAFs) within the tumor microenvironment (TME), though the precise molecular mechanisms remain unclear. This study aims to elucidate the role of CAFs in PDAC metastasis.

Methods: We integrated transcriptomic (GSE183795) and single-cell sequencing data (GSE156405) to screen for core genes associated with PDAC.

How Long Until Agricultural Carbon Peaks in the Three Gorges Reservoir? Insights from 18 Districts and Counties.

Under the global climate governance framework, the Paris Agreement and the China-U.S. Glasgow Joint Declaration established a non-negotiable target of limiting 21st-century temperature rise to 1.

Targeting microglia polarization with Chinese herb-derived natural compounds for neuroprotection in ischemic stroke.

Given that ischemic stroke ranks as one of the most fatal diseases globally, it is imperative to develop clinically effective neuroprotective agents for stroke. Microglia serve as innate immune cells for maintaining brain homeostasis, and upon activation, they are well-known to be able to transform into two functional phenotypes, namely, the M1 and M2 types, which can convert each other and exert opposing effects on neurotoxicity and neuroprotection, respectively. Traditional Chinese medicine possesses a deep-rooted and profound history with rich theory in treating cerebrovascular disorders, and its natural compounds have been considered as promising adjunctive therapies.

Chronic Lung Diseases and Depressive Symptoms in Older Adults: Insights from Observational Studies and Mendelian Randomization.

Introduction: To elucidate the currently unknown relationship between chronic lung diseases (CLDs) and depressive symptoms among older adults.

Methods: A total of 8079 older adults from China Longitudinal Aging Social Survey (CLASS) and 1119 individuals from a Sichuan Province cohort were included in this study. We used regression analysis and propensity score matching (PSM) to assess the association between CLDs and depressive symptoms among older adults, while the causal relationship was assessed using Mendelian randomization (MR).

Molecular Mechanism of Exogenous GABA in Regulating Salt Tolerance in Tomato ( L.).

To explore the mechanism by which γ-aminobutyric acid (GABA) regulates the response of different salt-sensitive tomato seedlings under salt stress conditions, we used the previously selected salt-sensitive tomato 'M82' and the salt-tolerant introgression line 'IL-7-5-5'. The following three treatments were set up: (1) a normal nutrient solution concentration as the control, (2) a nutrient solution with 200 mmol·L NaCl, and (3) a nutrient solution with 200 mmol·L NaCl and 35 mmol·L GABA. The concentration of the reactive oxygen species metabolism-related compounds and antioxidant enzyme activity in the leaves of tomato seedlings subjected to the different treatments were measured, and transcriptome and metabolome analyses were conducted.

Mechanisms and therapeutic potential of epithelial-immune crosstalk in airway inflammation.

Introduction: Chronic airway inflammatory diseases mainly comprise chronic rhinosinusitis (CRS), allergic rhinitis (AR), asthma, cystic fibrosis (CF), and chronic obstructive pulmonary disease (COPD). Epithelial cells fulfill a protective role as a barrier; however, when stimulated, these cells also release a variety of cytokines that attract and activate immune cells, including macrophages, neutrophils, and T-lymphocytes. Excessive activation and aggregation of immune cells disrupts the balance of the cellular microenvironment, and leads to impaired immune defense of the airway mucosa, which can further exacerbate an inflammatory response.

Assessment of left ventricular systolic function using pressure-strain loops in offspring of women with gestational diabetes mellitus: a prospective cohort study.

Background: Gestational diabetes mellitus (GDM) increases the risk of cardiovascular abnormalities in offspring. The objective of this study is to assess changes in left ventricular myocardial work using the left ventricular pressure-strain loop (LVPSL) method in neonates born to mothers with GDM. The aim of the research is to examine early impairments in neonatal left ventricular systolic function and to investigate whether these impairments persist over time.

Mimicking the process from secretory vesicles to organelles in eukaryotic cell evolution by clustering enzyme-liposomes in artificial cells.

Eukaryotic cells exhibit complex multicompartmentalized structures that optimize enzymatic efficiency as a result of continuous evolution. Inspired by this, we developed artificial cell models mimicking the evolutionary process from secretory vesicles to organelles using liquid-liquid phase separation (LLPS). An aqueous two-phase system (ATPS) enabled the creation of membrane-less compartments encapsulating liposomes loaded with glucose oxidase (GOx) and myoglobin (Mb).

Engineered cell nanovesicle antagonists for androgen deprivation therapy of melanoma.

Epidemiological studies on melanoma have revealed significant gender disparities, with the incidence and mortality rates being higher in males than in females. Recent studies indicate that androgen contributing to T cell exhaustion and promoting cancer cell proliferation. While clinical androgen deprivation therapies (ADT),particularly the use of androgen receptor (AR) antagonists to block AR signaling, has been employed in clinical settings to reduce androgen levels, antiandrogen drugs often encounter challenges such as poor targeting and selectivity, increased toxicity, low stability, short half-life and the emergence of drug resistance.

A highland-adaptation variant near MCUR1 reduces its transcription and attenuates erythrogenesis in Tibetans.

To identify genomic regions subject to positive selection that might contain genes involved in high-altitude adaptation (HAA), we performed a genome-wide scan by whole-genome sequencing of Tibetan highlanders and Han lowlanders. We revealed a collection of candidate genes located in 30 genomic loci under positive selection. Among them, MCUR1 at 6p23 was a novel pronounced candidate.

Identifying Hepsin as a novel biomarker for human esophageal squamous cell carcinoma (ESCC) and its application in fluorescence imaging.

Human Esophageal squamous cell carcinoma (ESCC) represents a type of malignant tumor characterized by a high mortality rate and a generally poor surgical prognosis. The accurate labeling and high-specificity visualization of ESCC cells is extremely importance for its precise diagnosis and effective treatment. Fluorescence molecular imaging has emerged as one of the most critical modalities for cancer detection and therapeutic guidance, owing to its superior sensitivity, cost-effectiveness, portability, real-time imaging, and no damage.

Pulsed electromagnetic fields modulate energy metabolism during wound healing process: an in vitro model study.

Background: Pulsed electromagnetic fields (PEMFs) therapy was extensively investigated to treat wound healing, which is a highly metabolically demanding process. However, the effect of PEMFs on energy metabolism in wound healing remains largely unexplored. Therefore, our study aims to demonstrate the role of PEMFs on energy metabolism in wound healing.

Small extracellular vesicles derived from miRNA-486 overexpressed dental pulp stem cells mitigate high altitude pulmonary edema through PTEN/PI3K/AKT/eNOS pathway.

High altitude pulmonary edema (HAPE) is a life-threatening, non-cardiogenic pulmonary edema characterized by rapid onset and high mortality. Extracellular vesicles of mesenchymal stem cells are used in the treatment of a variety of lung diseases, but their use in HAPE remains underreported. This study explores the therapeutic potential of miRNA-486 modified extracellular vesicles from dental pulp stem cells (sEV) against HAPE, aiming to decipher the associated molecular mechanisms.

Research on the Parameter Configuration Law of Transfer of Ultramicro Liquid by Pipetting Needle Based on the Quasi-static State.

Microscale device surface encapsulation needs to use ultrafine liquid transfer technology. This technology can transfer a liquid from a donor surface to a receptor surface in a controlled manner. However, the requirement of microscale encapsulation for liquid transfer amounts is generally at the pL level.

Unravelling distinct patterns of metagenomic surveillance and respiratory microbiota between two P1 genotypes of .

To unravel distinct patterns of metagenomic surveillance and respiratory microbiota between () P1-1 and P1-2 and to explore the impact of the COVID-19 pandemic on epidemiological features, we conducted a multicentre retrospective study which spanned 90,886 pneumonia patients, among which 3164 cases were identified. Our findings revealed a concurrent outbreak of , with the positivity rate rising sharply to 9.62% from July 2023, compared to the 0.

CMTM6 promotes hepatocellular carcinoma invasion and metastasis and tumor-associated neutrophil immunoinfiltration through the Wnt/β-catenin pathway.

Background: CMTM6 has been closely associated with the onset and progression of various tumor types. However, the precise mechanism by which CMTM6 operates in hepatocellular carcinoma remains elusive, necessitating further investigation.

Methods: Expression levels of CMTM6 in hepatocellular carcinoma tissues and cells were analyzed using immunohistochemistry and quantitative real-time PCR.