Proteomic and Functional Analysis Reveals Temperature-Driven Immune Evasion Strategies of in Yellowfin Seabream ().

() is a globally significant aquatic pathogen responsible for severe economic losses in aquaculture. While the infection often exhibits distinct seasonal patterns strongly correlated with water temperature, there is limited knowledge regarding the temperature-dependent immune evasion strategies of . Our results demonstrated a striking temperature-dependent virulence phenotype, with significantly higher mortality rates observed at high temperature (HT, 33 °C) compared to low temperature (LT, 23 °C).

UBE4B-driven suppression of megakaryocyte maturation by HBV: A novel mechanism linking viral infection to thrombocytopenia via p53-ERK1/2 crosstalk.

Background And Aim: Hepatitis B virus (HBV) infection can lead to thrombocytopenia through its effects on hematopoiesis, although the underlying mechanisms have not been fully elucidated. Platelet production involves multiple stages, including the differentiation of mature megakaryocytes, which plays a pivotal role. In this study, we assessed the variances in megakaryocyte differentiation and maturation after HBV infection and investigated the molecular mechanism involved.

Synergy of nanoclusters/single atoms regulates sustainable oxidation pathways for self-motivated Fenton-like processes.

Synergy mechanism of nanoclusters/single atoms as coexisting electron-rich centers for sustainable Fenton-like catalysis without oxidant addition remains unclear. In this study, a composite catalyst consisting of FeP nanoclusters coexisting with Fe single atoms (FeP/FeNP/C) was synthesized and utilized in two catalytic systems: one with zero-peroxymonosulfate (PMS) addition and the other with low-PMS addition. Results indicated that FeP/FeNP/C catalyst with electron-deficient region could adsorb pollutants via π-π bond and extract electrons from pollutants to the atomic FeNP/C as electron acceptor, and this self-motivated process in PMS-free system could be modulated by the FeP nanoclusters via dynamic synergistic effects.

User Satisfaction With Pregnancy Management Apps in Mainland China: User-Generated Content Analysis and Text Mining Study.

Background: China's 3-child policy has increased the demand for scientific and personalized pregnancy health management. The convenience of mobile health has promoted the use of pregnancy management apps among pregnant women. User satisfaction has a significant impact on continued use intention.

Nanosecond-scale single-molecule reaction dynamics for scalable synthesis on a chip.

Reaction mechanism studies typically involve the characterization of products, and intermediates are often characterized by (sub)millisecond techniques, such as nuclear magnetic resonance, while femto/attosecond spectroscopies are used to elucidate the evolution of transition states and electron dynamics. However, due to the lack of detection techniques in the microsecond to nanosecond range, as well as the emergent complexity with increasing scale, most of the proposed intermediates have not yet been detected, which significantly hinders reaction optimization. Here, we present such a nanosecond-scale real-time single-molecule electrical monitoring technique.

Chaos key update using constellation selection and clustering for data centers.

Key updating in data centers (DCs) is essential, but it presents challenges related to the consumption of channel resources. To address this issue, we propose a chaos key update scheme using constellation selection and clustering for DCs. At the transmitter side (DC1), the keys to be transmitted are encrypted by generating a digital chaotic sequence using a 4D hyperchaotic system to obtain encryption keys (EK).

Antidepressant in treating myocardial infarction complicated with depression via 5-HT/inflammation from heart to brain.

Objective: Exploring the potential mechanism by which inflammation and 5-HT linking myocardial infarction (MI) and depression, and to evaluate therapeutic effect of fluoxetine and SN50.

Methods: Patients with coronary artery disease (CAD) were recruited; depressive symptoms were evaluated, inflammatory factors were detected, and the effects of selective serotonin reuptake inhibitors (SSRIs) for CAD patients with depression were recorded. Furthermore, fluoxetine and SN50 were administrated separately for treatment in MI mice, SERT knockout mice, and H9C2 cell experiments.

Mechanistic insights into SUMOylation and its role in cancer pathogenesis: bridging oncogenesis, immunity, and therapeutic opportunities.

SUMOylation is a post-translational modification mediated by SUMO proteins, a family of small ubiquitin-like biological macromolecules that covalently attach to specific lysine residues in target proteins. This modification regulates various macromolecular functions, including transcriptional control, subcellular localization, DNA repair, and cell cycle progression. Recent advances have unveiled the multifaceted roles of SUMOylation in cancer pathogenesis, encompassing oncogenesis, immune modulation, and therapeutic resistance.

The Effects of Dietary Supplementation of Chestnut Tannic Acid on the Growth Performance, Gut Morphology and Microbiota of Weaned Piglets.

: This study investigated the effects of chestnut tannic acid (TA) on the growth performance, the expression of tight junction proteins and the composition of the gut microbiota of weaned piglets, which could provide novel insights into the application of TA in swine production. : In a 42-day trial, 180 healthy, 21-day-old Duroc × Landrace × Yorkshire piglets were randomly assigned to a Control group and four treatment groups (TA1-4), fed commercial diets supplemented with 0, 0.06%, 0.

FN1 from cancer-associated fibroblasts orchestrates pancreatic cancer metastasis via integrin-PI3K/AKT signaling.

Objective: The metastasis of pancreatic ductal adenocarcinoma (PDAC) is closely linked to the remodeling of cancer-associated fibroblasts (CAFs) within the tumor microenvironment (TME), though the precise molecular mechanisms remain unclear. This study aims to elucidate the role of CAFs in PDAC metastasis.

Methods: We integrated transcriptomic (GSE183795) and single-cell sequencing data (GSE156405) to screen for core genes associated with PDAC.

Long-read sequencing transforms the diagnosis of congenital adrenal hyperplasia: resolving pseudogene interference and structural variations.

Background: Congenital adrenal hyperplasia (CAH) is an autosomal recessive disorder primarily caused by defects in adrenal steroidogenesis. Conventional genetic methods struggle to resolve complex structural variations and pseudogene interference in key genes like . Our study will evaluate the efficacy of Long-Read Sequencing (LRS) as a comprehensive diagnostic tool for CAH, demonstrating its ability to simultaneously detect large structural variations, single nucleotide variants (SNVs), and small insertions or deletions.

Role of immunoglobulin M in Trachinotus ovatus defense against Cryptocaryon irritans infection.

Cryptocaryon irritans, a marine parasitic protozoan, poses a significant threat to marine teleosts, including golden pompano (Trachinotus ovatus) species. Studies show that T. ovatus can develop specific immunity following C.

Enhanced volatile methylsiloxanes degradation through designed enzyme local electric field.

Widespread application of man-made chemicals volatile methylsiloxanes (VMS) has caused a variety of environmental and health issues. Enzymatic degradation of VMS can be a promising eco-friendly solution. Recent advances in the directed evolution of cytochrome P450 have shown significant potential for VMS degradation.

Mechanical stretch-mediated fibroblast activation: The pivotal role of Piezo1 channels.

Mechanical forces are crucial in regulating fibroblast behavior, yet the underlying mechanisms remain unclear. This study aims to elucidate the role of the Piezo1 ion channel in fibroblast responses to mechanical stimulation. A mechanical stimulation culture platform was developed using a polydimethylsiloxane (PDMS)-based stretchable membrane and the Cell Tank uniaxial cell stretching system.

Integrated lncRNA and mRNA analysis reveals the immune modulatory mechanisms of antimicrobial peptide BSN-37 in mouse peritoneal macrophages.

Antimicrobial peptides (AMPs) possess vaccine adjuvant activity; however, their specific targets and molecular mechanisms remain incompletely understood, which hinders their clinical application. This study aimed to elucidate the key targets and pathways through which the antimicrobial peptide BSN-37 modulates immune responses in macrophages, providing evidence for its potential clinical translation. In this investigation, Balb/c mice were administered BSN-37 for 12 h, after which total RNA was extracted from peritoneal macrophages to assess the mRNA expression levels of cytokines and key molecules on the cell surface, followed by transcriptomic sequencing.

The impact of autonomy-supportive organizational environments on employees' emotions and creative performance: A self-determination theory perspective.

The ongoing debate over whether positive or negative emotions foster creative performance remains a pivotal issue in understanding the interplay between emotions and creativity. Emerging research suggests that both positive and certain negative emotions, such as fear and guilt, can enhance creativity under specific conditions. Grounded in Self-Determination Theory (SDT), this study examines how autonomy-supportive organizational environments contribute to the satisfaction of employees' basic psychological needs.

Synthesis of Co-Doped InO Hierarchical Porous Nanocubes for High-Performance Hydrogen Sulfide Sensors.

Recently, because of the urgent need for safety and health protection, there has been a growing focus on exploring effective and feasible gas sensors based on metal oxide semiconductors (MOSs) for detecting trace levels of hydrogen sulfide (HS). In this context, a cobalt (Co) doping strategy was proposed to improve the HS-sensitive properties of InO nanomaterials, enabling them to monitor 1 ppm of HS at a relatively lower temperature. The Co-doped InO hierarchical porous nanocubes (Co-InO HPNCs) were prepared through a hydrothermal route using In(OH) as a precursor.

Case Report: a novel homozygous variant in a Chinese female with severe microcephaly, encephalopathy and epilepsy.

Asparagine synthetase deficiency (ASNSD; OMIM# 615574) is a severe autosomal recessive neurodevelopmental disorder caused by biallelic pathogenic variants in (OMIM# 108370). Clinical features of ASNSD include congenital microcephaly, profound psychomotor impairment, progressive encephalopathy, refractory epilepsy, and characteristic neuroimaging abnormalities. Since its initial description, approximately 100 cases have been documented worldwide with 60 distinct variants reported.

Targeting BMPER as a therapeutic strategy for pulmonary arterial hypertension.

Pulmonary arterial hypertension is a life-threatening condition marked by pulmonary vascular remodeling, leading to increased pulmonary artery pressure and right heart hypertrophy. This chronic process involves excessive proliferation and migration of endothelial and smooth muscle cells. Our research, involving both pulmonary arterial hypertension patients and animal models, reveals reduced BMPER levels in cases of pulmonary arterial hypertension.

Modularized Cathode with Neural Network Topology for High Rate and Fault-Tolerant Lithium-Sulfur Batteries.

Enhancing the redox kinetics of electrodes, achieving synergistic optimization of local energy conversion and overall charge transfer, and overcoming the technical bottleneck of significant performance degradation due to local unit failure in traditional electrode systems are crucial for developing high-rate lithium-sulfur batteries. Here, a modular cathode system (CoBN-MR/FNN) with a fully connected cascade neural network topology (FNN) is designed by constructing microreactor modules (CoBN-MRs) with embedded nanozymes (Co-BN), ordering and efficiently interconnecting them. This system not only enables efficient energy conversion within individual microreactors but also significantly enhances the long-range charge transport efficiency and energy aggregation capacity of the electrodes.

Novel Frameshift Mutation as a Cause of Allan-Herndon-Dudley Syndrome and its Implications for Carrier Screening.

Background: Allan-Herndon-Dudley syndrome (AHDS) is a rare X-linked neurodevelopmental disorder caused by mutations in the solute carrier family 16-member 2 () gene. This syndrome leads to significant psychomotor disabilities, thyroid dysfunction, and abnormal brain development. This case report describes the genetic cause of AHDS in a male proband and to expanding the mutation spectrum of the gene.

Expression Profiles of lncRNAs and mRNAs in the Mouse Brain Infected with Pseudorabies Virus: A Bioinformatic Analysis.

Pseudorabies virus (PRV) is a pathogen that causes severe neurological dysfunction in the host, leading to extensive neuronal damage and inflammation. Despite extensive research on the neuropathogenesis of α-herpesvirus infections, many scientific questions remain unresolved, such as the largely unknown functions of long non-coding RNAs (lncRNAs) in herpesvirus-infected nervous systems. To address these questions, we used RNA sequencing (RNA-seq) to investigate the expression profiles of lncRNAs and mRNAs in the brains of mice infected with PRV.

Global trends and interdisciplinary insights in Tai Chi research: a bibliometric analysis (2004-2024).

Background: Tai Chi, as a traditional Chinese martial art, has received extensive attention in recent years due to its multiple health benefits. Research demonstrates that Tai Chi improves physical health, enhances flexibility and coordination, and alleviates psychological stress. With the increase in research, the health benefits of Tai Chi have been confirmed by many scientific studies.

Migration of an active particle in mixtures of rigid and flexible rings.

The migration of active particles in slowly moving, crowded, and heterogeneous media is fundamental to various biological processes and technological applications. In this study, we numerically investigate the dynamics of a single active particle in a multicomponent medium composed of mixtures of rigid and flexible rings. We observe a nonmonotonic dependence of diffusivity on the relative fraction of rigid to flexible rings, leading to the identification of an optimal composition for enhanced diffusion.