Effects of etomidate versus propofol for total intravenous anaesthesia on postoperative quality of recovery in patients undergoing day-case laparoscopic cholecystectomy: protocol for a multicentre, randomised controlled non-inferiority trial.

Background: The choice of anaesthetic agents may influence specific aspects of postoperative recovery, such as haemodynamic stability, recovery times and the incidence of adverse events, in patients undergoing day-case laparoscopic cholecystectomy. Propofol is widely used in total intravenous anaesthesia (TIVA) for its favourable recovery profile, while etomidate, valued for its haemodynamic stability, is less commonly used due to concerns about adrenal suppression. This study aims to compare etomidate-based and propofol-based TIVA on postoperative quality of recovery in patients undergoing day-case laparoscopic cholecystectomy, hypothesising that etomidate is non-inferior to propofol.

To fuse or not to fuse: surgical strategies for recurrent lumbar disc herniation from a 16-nation study.

Objective: Variations exist among surgeons in the treatment of recurrent lumbar disc herniation (LDH), generating major issues in decision-making models. The authors aimed to identify international nuances in surgical treatment patterns, highlight the differences in responses in each country group and different treatment trends across countries, and identify factors that influence surgical decisions.

Methods: An online survey with preformulated answers was submitted to 292 orthopedic surgeons and 223 neurosurgeons from 16 countries regarding 3 clinical vignettes (recurrence without low back pain, recurrence with severe low back pain, and recurrence with 2-level disc disease).

RNA binding protein SERBP1 is indispensable for oocyte development and maturation in mice.

RNA-binding proteins (RBPs) are critical regulators of post-transcriptional gene expression and RNA processing during mammalian oocyte development. SERPINE1 mRNA-binding protein 1 (SERBP1), a conserved RNA-binding protein (RBP), exhibits prominent expression in the female reproductive system and throughout oogenesis. Conditional deletion of Serbp1 using oocyte-specific Zp3⁠/⁠Gdf9⁠-Cre drivers resulted in arrested oocyte growth, female infertility, and failure of blastocyst formation from two-cell embryos.

RIPPLY1 suppresses cancer cell stemness via targeting TBX19 in CTNNB1-mutated hepatocellular carcinoma.

Background Aims: CTNNB1-mutated HCCs exhibit a relatively low stem-like and well-differentiated phenotype. However, the mechanism remains unclear. Ripply transcriptional repressor 1 (RIPPLY1), a transcriptional repressor required for somite segmentation, has hardly been studied in cancer.

Clinical and hemodynamic outcomes of side-to-side anastomosis in superficial temporal artery-middle cerebral artery bypass for adult patients with moyamoya disease: a prospective cohort study.

Objective: Superficial temporal artery-middle cerebral artery (STA-MCA) bypass, characterized by side-to-side (S-S) anastomosis, has been beneficial in reducing the incidence of postoperative complications and recurrent stroke in patients with moyamoya disease (MMD). However, the safety and efficacy of this unconventional S-S procedure remain unclear. This research aimed to investigate the clinical and hemodynamic outcomes associated with the S-S technique.

Defining region and duration-specific substantial clinical benefit values for the simplified Chinese lower extremity functional scale.

Background: A single substantial clinical benefit (SCB) value for the Simplified Chinese Lower Extremity Functional Scale (SC-LEFS) that can be applied to those with lower extremity musculoskeletal injuries is available. However, unique SCB values defined by specific body region and symptom duration may enhance the precision of the SC-LEFS in clinical assessments.

Methods: This study aim to determine unique SCB values for the SC-LEFS based on symptom duration and body region.

Epidemiology, risk factors, and antifungal susceptibility analysis of Candida tropicalis and non-C.tropicalis candidemia.

In recent years, the incidence of bloodstream infections has been increasing annually, drawing increasing clinical attention. This study aimed to investigate the epidemiological characteristics, risk factors, and antifungal susceptibility patterns of and non- species among patients with candidemia at Nanjing Drum Tower Hospital from March 2020 to February 2024. A retrospective analysis was conducted on 209 patients, revealing that accounted for 17.

Atherosclerosis increases adventitial pressure and limits solute transport via fluid-balance mechanisms.

The adventitia of blood vessels is their structural interface with surrounding tissues and may also contribute importantly to atherogenesis. Adventitial vasa vasorum and lymphatic vessels provide sources and sinks of interstitial fluid and solutes and remodel in disease. We constructed a mathematical model to investigate how soluble disease mediators, including lipoproteins and cytokines, are transported through the artery wall in healthy and atherosclerotic conditions.

Pen-2 regulates glial homeostasis by coordinating self-renewal and transdifferentiation programs in oligodendrocyte precursor cells.

Presenilin enhancer 2 (Pen-2) is implicated in neurological diseases characterized by glial dysregulation. To investigate whether oligodendrocytic γ-secretase activity is important for the maintenance of glial populations, we analyzed two conditional knockout (cKO) mouse models lacking Pen-2 or nicastrin. Both models exhibited similar expansions of oligodendrocyte precursor cells (OPCs) and astrocytes in the CNS.

Efficient 3D Biomedical Image Segmentation by Parallelly Multiscale Transformer-CNN Aggregation Network.

Accurate and automated segmentation of 3D biomedical images is a sophisticated imperative in clinical diagnosis, imaging-guided surgery, and prognosis judgment. Although the burgeoning of deep learning technologies has fostered smart segmentators, the successive and simultaneous garnering global and local features still remains challenging, which is essential for an exact and efficient imageological assay. To this end, a segmentation solution dubbed the mixed parallel shunted transformer (MPSTrans) is developed here, highlighting 3D-MPST blocks in a U-form framework.

Diet, genetic factors, and the risk of gastroesophageal reflux disease, Barrett's esophagus and esophageal adenocarcinoma.

: Diet has been recognized as the most pivotal modifiable lifestyle factor in the development of Gastroesophageal Reflux Disease (GERD), Barrett's Esophagus (BE) and Esophageal Adenocarcinoma (EA). However, the associations between dietary ingredients, patterns, and diet-gene interactions with the risk of GERD, BE and EA remain unclear. : The prospective cohort study included 502 412 participants from the UK Biobank.

Allogeneic CD19-targeting T cells for treatment-refractory systemic lupus erythematosus: a phase 1 trial.

Commercial autologous anti-CD19 chimeric antigen receptor-T cell therapies are effective in B cell malignancies and autoimmune diseases but are limited by personalized manufacturing, high costs and the risk from random chimeric antigen receptor insertion into the genome. To overcome these challenges, we developed YTS109, a hypoimmune allogeneic T cell product engineered using CRISPR-Cas9 to knock out TRAC, PD1, HLA-A, HLA-B and CIITA, with a CD19-targeting synthetic TCR and antigen receptor (STAR) precisely integrated into the TRAC locus to enable physiological, TCR-like signaling. As part of a multi-disease cohort trial, this article includes all enrolled five patients with severe, refractory systemic lupus erythematosus (SLE) complicated by lupus nephritis, who received lymphodepletion followed by YTS109 at 3 × 10 STAR⁺ T cells per kg body weight.

The G-branch off-the-shelf mixed branch endograft for thoracoabdominal aortic aneurysm: 1-year results from a prospective multicenter study.

Background: The use of off-the-shelf multi-branched endografts for thoracoabdominal aortic aneurysm (TAAA) is increasing; however, these commercially available devices have limited anatomical feasibility for treating TAAA. This study aimed to assess the safety and efficacy of a novel G-branch off-the-shelf endograft for TAAA.

Materials And Methods: A total of 73 patients with TAAA were treated using the G-branch endograft at 14 sites across China.

Mendelian randomization and single-cell analysis reveal causal relationships between renal clear cell carcinoma, kidney fibrosis, and inflammatory factors.

Background: Renal clear cell carcinoma (RCC) is the most common type of kidney cancer, and its relationship with kidney fibrosis and inflammatory responses has attracted considerable attention. However, whether causal relationships exist among these associations remains unclear, as traditional observational studies are susceptible to confounding factors. To evaluate causal relationships between kidney cancer, kidney fibrosis, and inflammatory factors using Mendelian randomization, and explore tumor microenvironment heterogeneity through single-cell analysis.

Improved Hypoxic Microenvironment By Nanoformulation For Effective T Cell Therapy In Mice Model.

Introduction: Adoptive cell therapy (ACT) has emerged as a powerful strategy for eliciting tumor regression. However, its efficacy in solid tumors remains limited, primarily due to the immunosuppressive tumor microenvironment (TME). We developed a tumor microenvironment-responsive mesoporous silica nanosphere (MSN) formulation co-loaded with the immunostimulant imiquimod (R837), zinc peroxide (ZnO), and manganese peroxide (MnO) to alleviate hypoxia and enhance dendritic cell (DC)-mediated antitumor immunity.

Antibiotic Susceptibility Evaluation in Bacterial Infections from Orthopedic Inpatients with Leg Trauma.

The COVID-19 pandemic has profoundly impacted healthcare delivery systems worldwide, but its impact on orthopedic infection patterns remains unclear. This retrospective comparative study analyzed 173 patients with leg trauma-related orthopedic infections from June 2018 to May 2025, divided into two periods based on China's pandemic control policy changes in December 2022: pre-policy adjustment (n = 86) and post-policy adjustment (n = 87). Our study revealed four major pandemic-driven changes: First, patients were significantly younger (50.

Effect of Hypertension on Long-term Adverse Clinical Outcomes and Liver Fibrosis Progression in MASLD.

Background & Aims: Hypertension is common in metabolic dysfunction-associated steatotic liver disease (MASLD), but its impact on long-term clinical outcomes and disease progression remains unclear. This study investigated the association of hypertension and risk of adverse clinical outcomes and progression of liver stiffness/fibrosis in MASLD.

Methods: Three multicenter prospective cohorts were analyzed: the UK BioBank (UKBB) cohort to assess the risk of adverse clinical outcomes, the VCTE-Prognosis cohort to assess liver stiffness/fibrosis progression, and the Paired Liver Biopsy cohort to assess histologic liver fibrosis progression.

Development and validation of a nomogram for predicting immunotherapy outcomes in lung cancer patients using clinical and blood biomarkers.

Background: Lung cancer remains the leading cause of cancer-related mortality worldwide. While immune checkpoint inhibitors (ICIs) have improved survival outcomes for some patients, their efficacy and adverse effects vary significantly. Thus, developing accurate and practical prognostic tools is essential to optimize treatment decision-making.

Genome-wide in vivo CRISPR screens identify GATOR1 complex as a tumor suppressor in Myc-driven lymphoma.

Identifying tumor suppressor genes is predicted to inform on the development of novel strategies for cancer therapy. To identify new lymphoma driving processes that cooperate with oncogenic MYC, which is abnormally highly expressed in ~70% of human cancers, we use a genome-wide CRISPR gene knockout screen in Eµ-Myc;Cas9 transgenic hematopoietic stem and progenitor cells in vivo. We discover that loss of any of the GATOR1 complex components - NPRL3, DEPDC5, NPRL2 - significantly accelerates c-MYC-driven lymphoma development in mice.

Thermodynamic landscapes of amino acid solvation within lipid bilayers: A comprehensive molecular dynamics study.

The interaction of amino acids with lipid bilayers dictates fundamental aspects of membrane protein folding, stability, and function. Quantifying the thermodynamics of amino acid solvation within the heterogeneous membrane environment remains a key challenge. Using extensive all-atom molecular dynamics simulations (>15 μs total) and enhanced sampling techniques, we calculated the potential of mean force profiles for all standard amino acids partitioning into a 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) bilayer, considering both neutral and charged states of ionizable residues.

An AND-gate DNA walker sensing microRNA and flap endonuclease 1.

Precise and sensitive detection of cancer biomarkers is essential for early diagnosis and effective treatment. However, most existing sensing strategies rely on single targets, which may lack the specificity required for accurate disease diagnosis. To address this limitation, we propose an AND-gate logic DNA walker capable of simultaneously detecting two distinct types of biomarkers: miRNA-21 and flap endonuclease 1 (FEN1).

ROLE OF CHANGES IN LIPOPROTEINS, LIPIDOMES, AND GUT MICROBIOTA IN THE PATHOGENESIS OF PERSISTENT INFLAMMATION, IMMUNOSUPPRESSION, AND CATABOLISM SYNDROME: A PROSPECTIVE COHORT STUDY.

Persistent inflammation, immunosuppression, and catabolism syndrome (PIICS) is a severe condition that occurs in patients in intensive care units (ICUs), and it is associated with high morbidity and mortality. This prospective cohort study investigated the dynamic changes in lipoproteins, lipidomics, and gut microbiota from days 1 to 7 posthospitalization for PIICS to elucidate their roles in the pathophysiology of PIICS. Patients admitted to the ICU were enrolled, and blood and fecal samples were collected 1 day and 7 days after admission.

Correction: Endothelial AIP1 regulates vascular remodeling by suppressing NADPH Oxidase-2.

[This corrects the article DOI: 10.3389/fphys.2018.