Effects of etomidate versus propofol for total intravenous anaesthesia on postoperative quality of recovery in patients undergoing day-case laparoscopic cholecystectomy: protocol for a multicentre, randomised controlled non-inferiority trial.

Background: The choice of anaesthetic agents may influence specific aspects of postoperative recovery, such as haemodynamic stability, recovery times and the incidence of adverse events, in patients undergoing day-case laparoscopic cholecystectomy. Propofol is widely used in total intravenous anaesthesia (TIVA) for its favourable recovery profile, while etomidate, valued for its haemodynamic stability, is less commonly used due to concerns about adrenal suppression. This study aims to compare etomidate-based and propofol-based TIVA on postoperative quality of recovery in patients undergoing day-case laparoscopic cholecystectomy, hypothesising that etomidate is non-inferior to propofol.

To fuse or not to fuse: surgical strategies for recurrent lumbar disc herniation from a 16-nation study.

Objective: Variations exist among surgeons in the treatment of recurrent lumbar disc herniation (LDH), generating major issues in decision-making models. The authors aimed to identify international nuances in surgical treatment patterns, highlight the differences in responses in each country group and different treatment trends across countries, and identify factors that influence surgical decisions.

Methods: An online survey with preformulated answers was submitted to 292 orthopedic surgeons and 223 neurosurgeons from 16 countries regarding 3 clinical vignettes (recurrence without low back pain, recurrence with severe low back pain, and recurrence with 2-level disc disease).

RNA binding protein SERBP1 is indispensable for oocyte development and maturation in mice.

RNA-binding proteins (RBPs) are critical regulators of post-transcriptional gene expression and RNA processing during mammalian oocyte development. SERPINE1 mRNA-binding protein 1 (SERBP1), a conserved RNA-binding protein (RBP), exhibits prominent expression in the female reproductive system and throughout oogenesis. Conditional deletion of Serbp1 using oocyte-specific Zp3⁠/⁠Gdf9⁠-Cre drivers resulted in arrested oocyte growth, female infertility, and failure of blastocyst formation from two-cell embryos.

RIPPLY1 suppresses cancer cell stemness via targeting TBX19 in CTNNB1-mutated hepatocellular carcinoma.

Background Aims: CTNNB1-mutated HCCs exhibit a relatively low stem-like and well-differentiated phenotype. However, the mechanism remains unclear. Ripply transcriptional repressor 1 (RIPPLY1), a transcriptional repressor required for somite segmentation, has hardly been studied in cancer.

Defining region and duration-specific substantial clinical benefit values for the simplified Chinese lower extremity functional scale.

Background: A single substantial clinical benefit (SCB) value for the Simplified Chinese Lower Extremity Functional Scale (SC-LEFS) that can be applied to those with lower extremity musculoskeletal injuries is available. However, unique SCB values defined by specific body region and symptom duration may enhance the precision of the SC-LEFS in clinical assessments.

Methods: This study aim to determine unique SCB values for the SC-LEFS based on symptom duration and body region.

Epidemiology, risk factors, and antifungal susceptibility analysis of Candida tropicalis and non-C.tropicalis candidemia.

In recent years, the incidence of bloodstream infections has been increasing annually, drawing increasing clinical attention. This study aimed to investigate the epidemiological characteristics, risk factors, and antifungal susceptibility patterns of and non- species among patients with candidemia at Nanjing Drum Tower Hospital from March 2020 to February 2024. A retrospective analysis was conducted on 209 patients, revealing that accounted for 17.

Efficient 3D Biomedical Image Segmentation by Parallelly Multiscale Transformer-CNN Aggregation Network.

Accurate and automated segmentation of 3D biomedical images is a sophisticated imperative in clinical diagnosis, imaging-guided surgery, and prognosis judgment. Although the burgeoning of deep learning technologies has fostered smart segmentators, the successive and simultaneous garnering global and local features still remains challenging, which is essential for an exact and efficient imageological assay. To this end, a segmentation solution dubbed the mixed parallel shunted transformer (MPSTrans) is developed here, highlighting 3D-MPST blocks in a U-form framework.

Diet, genetic factors, and the risk of gastroesophageal reflux disease, Barrett's esophagus and esophageal adenocarcinoma.

: Diet has been recognized as the most pivotal modifiable lifestyle factor in the development of Gastroesophageal Reflux Disease (GERD), Barrett's Esophagus (BE) and Esophageal Adenocarcinoma (EA). However, the associations between dietary ingredients, patterns, and diet-gene interactions with the risk of GERD, BE and EA remain unclear. : The prospective cohort study included 502 412 participants from the UK Biobank.

The G-branch off-the-shelf mixed branch endograft for thoracoabdominal aortic aneurysm: 1-year results from a prospective multicenter study.

Background: The use of off-the-shelf multi-branched endografts for thoracoabdominal aortic aneurysm (TAAA) is increasing; however, these commercially available devices have limited anatomical feasibility for treating TAAA. This study aimed to assess the safety and efficacy of a novel G-branch off-the-shelf endograft for TAAA.

Materials And Methods: A total of 73 patients with TAAA were treated using the G-branch endograft at 14 sites across China.

Improved Hypoxic Microenvironment By Nanoformulation For Effective T Cell Therapy In Mice Model.

Introduction: Adoptive cell therapy (ACT) has emerged as a powerful strategy for eliciting tumor regression. However, its efficacy in solid tumors remains limited, primarily due to the immunosuppressive tumor microenvironment (TME). We developed a tumor microenvironment-responsive mesoporous silica nanosphere (MSN) formulation co-loaded with the immunostimulant imiquimod (R837), zinc peroxide (ZnO), and manganese peroxide (MnO) to alleviate hypoxia and enhance dendritic cell (DC)-mediated antitumor immunity.

Antibiotic Susceptibility Evaluation in Bacterial Infections from Orthopedic Inpatients with Leg Trauma.

The COVID-19 pandemic has profoundly impacted healthcare delivery systems worldwide, but its impact on orthopedic infection patterns remains unclear. This retrospective comparative study analyzed 173 patients with leg trauma-related orthopedic infections from June 2018 to May 2025, divided into two periods based on China's pandemic control policy changes in December 2022: pre-policy adjustment (n = 86) and post-policy adjustment (n = 87). Our study revealed four major pandemic-driven changes: First, patients were significantly younger (50.

Development and validation of a nomogram for predicting immunotherapy outcomes in lung cancer patients using clinical and blood biomarkers.

Background: Lung cancer remains the leading cause of cancer-related mortality worldwide. While immune checkpoint inhibitors (ICIs) have improved survival outcomes for some patients, their efficacy and adverse effects vary significantly. Thus, developing accurate and practical prognostic tools is essential to optimize treatment decision-making.

An AND-gate DNA walker sensing microRNA and flap endonuclease 1.

Precise and sensitive detection of cancer biomarkers is essential for early diagnosis and effective treatment. However, most existing sensing strategies rely on single targets, which may lack the specificity required for accurate disease diagnosis. To address this limitation, we propose an AND-gate logic DNA walker capable of simultaneously detecting two distinct types of biomarkers: miRNA-21 and flap endonuclease 1 (FEN1).

ROLE OF CHANGES IN LIPOPROTEINS, LIPIDOMES, AND GUT MICROBIOTA IN THE PATHOGENESIS OF PERSISTENT INFLAMMATION, IMMUNOSUPPRESSION, AND CATABOLISM SYNDROME: A PROSPECTIVE COHORT STUDY.

Persistent inflammation, immunosuppression, and catabolism syndrome (PIICS) is a severe condition that occurs in patients in intensive care units (ICUs), and it is associated with high morbidity and mortality. This prospective cohort study investigated the dynamic changes in lipoproteins, lipidomics, and gut microbiota from days 1 to 7 posthospitalization for PIICS to elucidate their roles in the pathophysiology of PIICS. Patients admitted to the ICU were enrolled, and blood and fecal samples were collected 1 day and 7 days after admission.

Evaluating the Oxidative Balance Score for Peripheral Artery Disease Risk: Integrating Epidemiologic Modeling and SHAP-Interpretable Machine Learning in NHANES.

Oxidative stress plays a central role in the development of peripheral artery disease (PAD), yet composite indices quantifying its impact remain underutilized. The oxidative balance score (OBS), integrating dietary and lifestyle exposures, may offer a comprehensive approach to vascular risk stratification. We analyzed data from 7249 U.

HPVPool-Seq: a genotype-guided pooling strategy for cost-effective next-generation sequencing detection of HPV integration in cervical samples.

Unlabelled: Integration of high-risk human papillomavirus (hrHPV) DNA is a critical event in carcinogenesis and a promising biomarker for risk stratification. However, the high cost of next-generation sequencing (NGS) limits its widespread clinical adoption. We developed HPVPool-Seq, an innovative pooling strategy that leverages the inherent diversity of HPV genotypes as natural barcodes, enabling cost-effective, scalable integration detection.

Mitochondria-Targeted Ferroptosis Nanodrug for Triple-Negative Breast Cancer Therapy via Fatty Acid Metabolism Remodeling and Tumor Bacterial Symbiosis Inhibition.

Triple-negative breast cancer (TNBC) is considered one of the most aggressive subtypes of breast cancer, due to its pronounced propensity for metastasis. This challenge is amplified by the critical role of mitochondria in metastasis, regulating processes like fatty acid metabolism that drive tumor cell migration. Moreover, emerging evidence suggests that bacterial infiltration, particularly Staphylococcus xylosus (S.

Romantic relationships and adolescent depression in China: moderating effects of peer norms.

Objective: Adolescent depression is a crucial determinant of an individual's developmental trajectories and long-term health-related quality of life. Romantic relationships are widely recognized as a risk factor of adolescent depression. Peer norms, as an important source of social influence in adolescence, have the potential to moderate the effects of romantic relationships.

Proximity cleavage assay (PCA): A single-step, 20-minute platform for protein detection in 1-μL biofluids.

Rapid and accurate detection of protein biomarkers from tiny body fluid samples (approximately microliters) is required for both point-of-care testing (POCT) and at-home testing, but most protein biomarker assays struggle to combine small sample size, rapidity, simplicity of operation, and high sensitivity at the same time. In this paper, we proposed a proximity cleavage assay (PCA) for the highly sensitive and one-pot detection of protein biomarkers with 1-μL biological samples. Employing dual ligands labeled with DNA tags as probes, this approach triggers a cascaded signal amplification reaction upon target binding, completing the detection process within 20 min and requiring only one manual operation step of sample spiking and mixing.

A nine-gene signature with potential targets for predicting the prognosis of patients with esophageal cancer.

Background: The incidence of esophageal squamous cell carcinoma (ESCC) is high and the prognosis is poor. It has become one of the important factors threatening the economic development and social stability of the region. The lack of effective early diagnostic biological indicators is the main reason for the late visit time and high mortality rate of clinical patients with ESCC.