CPK12 decodes effector-triggered calcium signaling and phosphorylates PIP2;1 to facilitate apoplastic ROS transport into the cytoplasm in Arabidopsis.

Effector-triggered immunity (ETI) in plants is mediated by intracellular nucleotide-binding leucine-rich repeat receptors (NLRs), which converge on calcium (Ca) signaling pathways. However, how NLR-induced Ca signals initiate downstream immune responses, such as enhancing reactive oxygen species (ROS) signaling, remains largely unclear. In this study, we identified a calcium-dependent protein kinase (CPK) that regulates sustained ETI-ROS signaling.

RIPPLY1 suppresses cancer cell stemness via targeting TBX19 in CTNNB1-mutated hepatocellular carcinoma.

Background Aims: CTNNB1-mutated HCCs exhibit a relatively low stem-like and well-differentiated phenotype. However, the mechanism remains unclear. Ripply transcriptional repressor 1 (RIPPLY1), a transcriptional repressor required for somite segmentation, has hardly been studied in cancer.

Targeting xanthine oxidoreductase reverses resistance to EGFR tyrosine kinase inhibitors in intrahepatic cholangiocarcinoma.

Background & Aims: Despite the overexpression and aberrant activation of epidermal growth factor receptor (EGFR) in intrahepatic cholangiocarcinoma (iCCA), the disease remains refractory to EGFR tyrosine kinase inhibitors (TKIs). Multiple clinical trials involving EGFR-targeting agents have been conducted; however, none have demonstrated clinically significant efficacy. The aim of this study was to elucidate the mechanisms underlying EGFR TKI resistance in iCCA.

Gasdermin-D-mediated epithelial-immune circuit synchronizes nutrient absorption and host defense in the small intestine.

The small intestine (SI) absorbs nutrients and acts as a barrier against pathogens. Diet enables the absorptive function of the SI while maintaining immune homeostasis. But how the SI transmits nutritional signals to the immune response and adapts to dietary intake remains unclear.

The deficiency of chymase mast cell protease 4 exacerbates dextran sulfate sodium salt-induced colitis in mice and is associated with altered microbiota and metabolome profiles.

Introduction: Ulcerative colitis (UC) is a chronic gastrointestinal disease characterized by symptoms of abdominal pain and diarrhea. Chymase is a serine protease released by the mast cells and is highly expressed in patients with UC. However, its role in disease pathogenesis remains poorly understood.

Assessment of malnutrition risk and analysis of influencing factors in patients with chronic kidney disease: A cross-sectional survey.

Objective: To assess the status of malnutrition risk in patients with chronic kidney disease (CKD) using objective nutritional indices and to analyze the influencing factors.

Methods: 1277 patients with CKD admitted to the Department of Nephrology at a Class A hospital in Nanjing from 2020 to 2022, were selected for this study. The Prognostic Nutritional Index (PNI) and Controlling Nutritional Status (CONUT) were used to evaluate the risk of malnutrition.

Comparing the efficacy of topical interventions for pain management in oral lichen planus: a time-stratified bayesian network analysis of randomized controlled trials.

Background: The evaluation of short-term pain relief provided by newly developed drugs for the treatment of oral lichen planus is currently limited. The primary aim of this study was to establish a clinically relevant hierarchy among different treatments in terms of their effectiveness for short-term pain control.

Methods: EMBASE, Scopus, PubMed, WanFang database, and Cochrane databases were searched up to January 2025.

N-methyladenosine reader YTHDF3-mediated CEBPA translation maintains genomic stability and stem cell function to prevent liver injury.

Liver injury is a major health issue with significant implications for liver function and overall well-being, but precise mechanisms of the N-methyladenine (mA) reader YTHDF3 in liver injury remain severely understudied. Here, we discovered that Ythdf3 knockout exacerbated CCL-induced liver injury with a reduction in functional hepatocytes and liver stem cells using single cell RNA-sequencing and organoid culture. Furthermore, Mettl14 and YTHDF3-dependent RNA mA dysregulation induced DNA damage.

Exploring knowledge and attitudes toward electronic informed consent among clinical trial participants in China: a cross-sectional study.

Background: With the extensive integration of digital technology into clinical research, intelligence, virtualization, and decentralization have gradually transformed into emerging clinical research modes, the electronization of informed consent has become indispensable to the development of clinical trial informatization, and the inclination to use electronic informed consent (eIC) has grown. The knowledge and perceptions of research participants, as objects of informed consent acquisition, regarding eIC are crucial. However, few studies have empirically explored such issues.

Kindlin-2 silencing promoted apoptosis and cell cycle arrest through the fas/FasL pathway in hepatocellular carcinoma.

Objective: This study aimed to investigate the role of Kindlin-2 in HCC and its underlying molecular mechanisms, focusing on its regulation of the Fas/FasL signaling pathway.

Materials And Methods: In vitro, Hep3B and HepG2 cells were treated with Kindlin-2 siRNA, a Fas activator, and a combination of Kindlin-2 siRNA and Fas siRNA. Cell proliferation, apoptosis, and cell cycle progression were evaluated using CCK-8 assays and flow cytometry, while the expression of associated proteins was analyzed through Western blotting.

Correcting microplastic pollution and risk assessment in Chinese watersheds.

Microplastics (MPs) are emerging pollutants that are attracting attention because of their potential threats posed and their widespread presence in the environment. MP pollution in Chinese watersheds requires assessment; however, existing risk models face data-scale biases. By compiling 2,474 samples from 165 articles, we constructed a national dataset on MPs and propose a novel framework that integrates rescaled MP concentrations with MP characteristics to recalibrate MP pollution and ecological risks.

Innovative composite systems for enhancing plant polyphenol stability and bioavailability.

Plant polyphenols, secondary metabolites in plants, possess valuable functional properties, including anti-inflammatory, antioxidative, and hypoglycemic effects. However, their application in food products is often limited by chemical instability. This review examines innovative composite delivery systems designed to enhance the stability and bioavailability of plant polyphenols.

The effects of fermented polysaccharides on the growth performance, antioxidant capacity and intestinal health of broilers.

This study aimed to investigate the effects of fermented polysaccharides(FAP) on the growth performance, antioxidant capacity and intestinal health of broilers. A total of 1,080 Cyan-shank Partridge chickens were divided into 4 groups, with 6 replicates per group and 45 chickens per replicate. Add 0% (T1), 0.

ent-Atisane Diterpenoids with Anti-Influenza Virus Activity from Mangrove Plant Excoecaria agallocha L.

Three new ent-atisane diterpenoids, named agallolides N‒P (1-3) together with nine known diterpenoids (4-12) were isolated from the stems and twigs of the mangrove plant, Excoecaria agallocha L. . Various techniques, including high-resolution electrospray ionization mass spectrometry, spectroscopic methods, C-NMR calculations, DP4+ probability analysis, and equivalent circulating density calculations were applied to determine their molecular structures.

The role of potential oxidative biomarkers in the prognosis of intracerebral hemorrhage and the exploration antioxidants as possible preventive and treatment options.

Intracerebral hemorrhage (ICH) is a non traumatic hemorrhage that occurs in a certain part of the brain. It usually leads to brain cell damage. According to a large number of experimental research, oxidative stress is an important pathophysiological processes of cerebral hemorrhage.

Protocol for extraction of gut interstitial fluid in mice with two-front nutrient supply.

The intestine features a two-front nutrient supply environment, comprising an enteral side enriched with microbial and dietary metabolites and a serosal side supplied by systemic nutrients, collectively supporting intestinal and systemic homeostasis, but there is currently no optimal approach for extracting and assessing the local intestinal microenvironment. Here, we present a protocol for constructing a nutrient supply model in mice and extracting gut interstitial fluid (GIF) via centrifugation. This model and the extracted GIF are suitable for downstream analyses.

Association between the healthy eating index 2020 and heart failure among the U.S. middle-aged and older adults from NHANES 2005-2020: a cross-sectional study.

Objective: This study aims to shed light on the correlation between Healthy Eating Index-2020 (HEI-2020) and heart failure (HF) in American adults aged 50 or above.

Methods: Data were from the National Health and Nutrition Examination Survey 2005-2020, encompassing 13,105 participants with an age of 50 or above. HEI-2020 score was utilized for rating the dietary quality.

Energetic Materials Photolysis Footprint in High-Order Harmonic Generation.

Photolysis of energetic materials offers safer and more controllable advantages compared to traditional ignition methods. Tracking the group and electron dynamics during the photolysis of energetic materials is currently a hot and challenging topic. In this work, we used a time-dependent density functional theory (TDDFT) to study the high-order Harmonic generation (HHG) dynamics induced by strong laser interaction with an isolated CHNO molecule with varying C-N bond lengths.

Upregulation of OGT-mediated EZH2 O-GlcNAcylation Promotes Human Umbilical Vein Endothelial Cell Proliferation, Invasion, Migration, and Tube Formation in Gestational Diabetes Mellitus.

O-linked N-acetylglucosamine transferase (OGT)-catalyzed O-linked N-acetylglucosamine glycosylation (O-GlcNAcylation) is closely associated with diabetes progression. This study aims to investigate the mechanism of OGT in regulating endothelial dysfunction in gestational diabetes mellitus (GDM). Expressions of OGT, O-linked N-acetylglucosamine (O-GlcNAc), enhancer of zeste homolog 2 (EZH2), and HEK27me3 in human umbilical vein endothelial cells (HUVECs) and GDM-derived HUVECs (GDM-HUVECs) were assessed by western blot.

Progress of Immune Checkpoint Inhibitors Therapy for pMMR/MSS Metastatic Colorectal Cancer.

Immunotherapy is one of the research hotspots in colorectal cancer field in recent years. The colorectal cancer patients with mismatch repair-deficient (dMMR) or high microsatellite instability (MSI-H) are the primary beneficiaries of immunotherapy. However, the vast majority of colorectal cancers are mismatch repair proficient (pMMR) or microsatellite stability (MSS), and their immune microenvironment is characterized by "cold tumors" that are generally insensitive to single immunotherapy based on immune checkpoint inhibitors (ICIs).

Efficient removal of estradiol using MnFeO microsphere and potassium persulfate complex salt.

In this study, MnFeO microspheres were synthesized to activate potassium persulfate complex salt (Oxone) for the degradation of 17β-estradiol (17β-E2) in aqueous solutions. The characteristic of MnFeO was detected by XRD, XPS and SEM-EDS. The experimental results indicated that the degradation of 17β-E2 followed pseudo-first-order kinetics.