The m6A modification regulates the composition of myofiber types in chicken skeletal muscle.

As a widespread epigenetic RNA modification, N6-methyladenosine (m6A) plays essential regulatory roles in multiple biological processes. However, its function in maintaining and modulating myofiber-type properties remains largely unknown. To investigate the post-transcriptional modification underlying the myofiber type diversity in chicken skeletal muscle, we evaluated the m6A methylation levels of chicken skeletal muscles with different phenotypic traits, and profiled a transcriptome-wide m6A map in the oxidative and glycolytic skeletal muscles by methylated RNA immunoprecipitation sequencing (MeRIP-seq).

Marek's disease virus-1 unique gene LORF1 is involved in viral replication and MDV-1/Md5-induced atrophy of the bursa of Fabricius.

Marek's disease virus (MDV), an alphaherpesvirus, causes severe immunosuppression and T cell lymphomas in chickens, known as Marek's disease (MD), an economically important poultry disease primarily controlled by vaccination. Importantly, it also serves as a comparative model for studying herpesvirus-induced tumor formation in humans. MDV encodes more than 100 genes, most of which have unknown functions.

Effects of water acidifiers on the growth performance, intestinal function and gut microflora in broilers.

1. This study evaluated the effect of acidified drinking water on the gastrointestinal function and intestinal health of broilers.2.

Goose multi-omics database: A comprehensive multi-omics database for goose genomics.

Multi-omics has helped elucidate the gene expression patterns and genomic variations closely associated with economically significant traits in geese. Despite the substantial genomic data generated through extensive goose studies, a unified platform for integrating these datasets is lacking. To address this gap, we introduced the Goose Multi-omics Database (GMD), which is accessible at http://goosedb.

The Impact of Genetic Variation on Duck Hepatitis A Virus (DHAV) Vaccine Efficacy: A Comparative Study of DHAV-1 and DHAV-3 Against Emerging Variant Strains.

Duck virus hepatitis (DVH), caused by duck hepatitis A virus (DHAV), poses significant challenges to duck farming due to high mortality rates in young ducklings. Despite the widespread use of live attenuated vaccines, the genetic diversity within DHAV strains has diminished their cross-protection efficacy. This study aimed to evaluate the cross-protective efficacy of current DHAV-1 and DHAV-3 vaccines against genetically divergent wild strains.

Epidemiological Investigation and Genetic Analysis of Duck Circovirus in Korea from 2013 to 2022.

Duck circovirus (DuCV) infections cause immunosuppression in ducks, potentially leading to significant economic losses for the duck farming industry. This study investigates the prevalence, genetic characteristics, and evolutionary trends of DuCV in Korea between 2013 and 2022. Samples from 184 farms across seven provinces were analyzed using polymerase chain reaction (PCR).

Association between egg consumption and risk of obesity: A comprehensive review: EGG CONSUMPTION AND OBESITY.

Eggs serve as a vital source of high-quality protein and lipids in the human diet, contributing significantly to nutritional intake; however, the relation between egg intake and health risks has been controversial. This study aimed to assess the relationship between egg intake and obesity and the effects of the various nutrients in eggs on obesity were separately investigated. This review involved searching Scopus, PubMed, Google Scholar for relevant articles from 2002 to 2022.

Prevalence, detection of virulence genes and antimicrobial susceptibility of isolated from arbor acres broilers feeding cycle in China.

The prevalence of antimicrobial resistance originating from animals presents a significant threat to the treatment of animal disease, public health, and food safety. Researchers have focused on antibiotic resistance in (), yet there are few reports on the resistance change during the feeding cycle. The purpose of this study was to investigate the prevalence and antibiotic resistance changes of in animal, environmental, and human samples during the broiler feeding cycle.

Genome-wide association study of myofiber type composition traits in a yellow-feather broiler population.

Meat quality is a key factor determining the economic viability of the broiler industry, particularly in native broiler breeds. Skeletal muscles contain a mixture of muscle fibers, each possessing unique physicochemical properties; the composition of myofiber types within these muscles is closely linked to meat quality. However, comprehension of the regulatory mechanisms governing this trait remains limited.

Elaborate Structural Modifications Yielding Novel Boron-Containing N-Substituted Oseltamivir Derivatives as Potent Neuraminidase Inhibitors with Significantly Improved Broad-Spectrum Antiresistance Profiles.

Inspired by our previous finding that targeting the 150-cavity with a multisite-binding strategy emerged as an effective approach to obtain more potent and selective neuraminidase (NA) inhibitors against influenza virus, we present here the design, synthesis, and optimization of novel boron-containing N-substituted oseltamivir (OSC) derivatives. Exploratory structure-activity relationship (SAR) studies led to the identification of compounds and as the most potent NA inhibitors, surpassing OSC in potency against both wild-type group-1 NAs and oseltamivir-resistant NAs. These compounds demonstrated significant antiviral activity against several wild-type strains and H1N1pdm09 strains (EC = 0.

Cross-species single-cell analysis reveals divergence and conservation of peripheral blood mononuclear cells.

Background: Single-cell transcriptome sequencing (scRNA-seq) has revolutionized the study of immune cells by overcoming the limitations of traditional antibody-based identification and isolation methods. This advancement allows us to obtain comprehensive gene expression profiles from a diverse array of vertebrate species, facilitating the identification of various cell types. Comparative immunology across vertebrates presents a promising approach to understanding the evolution of immune cell types.

Identification of Single Nucleotide Polymorphisms Through Genome-Wide Association Studies of pH Traits in Goose Meat.

The genetic regulation of goose meat quality traits remains relatively unexplored, and the underlying mechanisms are yet to be elucidated. This study aims to employ single nucleotide polymorphism (SNP) genotyping in conjunction with genome-wide association studies (GWAS) to investigate critical candidate regions and genes associated with the pH trait of meat in Sichuan white geese. A cohort of 203 healthy male Sichuan white geese was randomly selected and slaughtered at 70 days of age.

Effects of 1-Deoxynojirimycin Extracts of Mulberry Leaves on Oxidative Stress and the Function of the Intestinal Tract in Broilers Induced by HO.

The poultry industry struggles with oxidative stress affecting gut health and productivity. This study examined using 1-Deoxynojirimycin (DNJ) extracts from mulberry leaves as an antioxidant in broilers feed to combat this issue. We divided 240 broilers, aged 16 days, into six groups, including a control and groups exposed to oxidative stress through HO injections, with different supplement levels of DNJ-E (40, 80, 120, and 160 mg/kg of the basal diet) lasting until the broilers reached 42 days old.

Lack of Serological and Molecular Evidence of Duck Tembusu Virus Infection in Ducks from South Korea.

The duck Tembusu virus (DTMUV), an emerging flavivirus, has led to severe neurological disorders and substantial economic losses in the duck industry throughout Asia. Considering South Korea's increasing duck production and its strategic location along the East Asian-Australasian Flyway, this study aimed to assess the presence of DTMUV in South Korea to evaluate potential risks to the poultry industry. We performed a comprehensive serological survey of 1796 serum samples from broiler and breeder ducks collected between 2011 and 2023, alongside molecular detection tests on 51 duck flocks exhibiting suspected clinical signs of DTMUV infection.

Responses of laying performance, eggshell quality, calcium, and phosphorus metabolism to feeding patterns and dietary available phosphorus levels in aged laying hens.

This study aimed to determine the effects of feeding patterns and dietary available phosphorus levels on laying performance, eggshell quality, calcium, and phosphorus metabolism in aged laying hens. The experiment followed a 3 × 2 factorial arrangement of three feeding patterns (feeding twice a day, 1/2 daily feeding at 8:00, and 1/2 daily feeding at 14:00; disequilibrium feeding twice a day, 1/3 daily feeding at 8:00, and 2/3 daily feeding at 14:00; feeding thrice a day, 1/3 daily feeding at 8:00, 1/3 daily feeding at 14:00, and 1/3 daily feeding at 18:00) and two available phosphorus levels (0.32 %, 0.

Design, synthesis, and biological evaluation of novel pleuromutilin derivatives with methicillin-resistant Staphylococcus aureus -targeting phenol linker groups.

Methicillin-resistant Staphylococcus aureus (MRSA) remains a significant global health threat, necessitating the development of new therapeutic agents. Pleuromutilin derivatives offer a promising solution due to their potent antibacterial activity, particularly against Gram-positive bacteria such as MRSA. In this study, we synthesized a series of pleuromutilin derivatives with phenol linker containing C14 side chains and evaluated in vitro and in vivo antibacterial activities.

METTL16 inhibits differentiation and promotes proliferation and slow myofibers formation in chicken myoblasts.

N6-methyladenosine (m6A) plays a crucial regulatory role in muscle growth and development. In our previous studies, we identified a m6A methyltransferase, Methyltransferase like 16 (METTL16), which is associated with chicken muscle development and muscle fiber type conversion. To further understand the regulatory role of METTL16 in chicken muscle function, we analyzed its expression in muscle tissues with different myofiber type compositions and in chicken primary myoblasts (CPMs) at various stages.

Unraveling the gut microbiota of Tibetan chickens: insights into highland adaptation and ecological advantages.

Unlabelled: Tibetan animals have several unique advantages owing to the harsh ecological conditions under which they live. However, compared to Tibetan mammals, understanding of the advantages and underlying mechanisms of the representative high-latitude bird, the Tibetan chicken (, TC), remains limited. The gut microbiota of animals has been conclusively shown to be closely related to both host health and host environmental adaptation.

Dynamic Transcriptome Profile Analysis of Mechanisms Related to Melanin Deposition in Chicken Muscle Development.

The pectoral muscle is an important component of skeletal muscle. The blackness of pectoral muscles can directly affect the economic value of black-boned chickens. Although the genes associated with melanogenesis in mammals and birds have been thoroughly investigated, only little is known about the key genes involved in muscle hyperpigmentation during embryonic development.

Age-dependent genetic architectures of chicken body weight explored by multidimensional GWAS and molQTL analyses.

Chicken body weight (BW) is a critical trait in breeding. Although genetic variants associated with BW have been investigated by genome-wide association studies (GWAS), the contributions of causal variants and their molecular mechanisms remain largely unclear in chickens. In this study, we construct a comprehensive genetic atlas of chicken BW by integrative analysis of 30 age points and 5 quantitative trait loci (QTL) across 27 tissues.

Antimicrobial activity, safety and pharmacokinetics evaluation of PMTM: A novel pleuromutilin candidate.

The prevalence of infections by methicillin-resistant Staphylococcus aureus (MRSA) has led to dramatically increased mortality and threated the public health worldwide. Pleuromutilin compound 14-O-[(4-(pyrrolidine-1-yl)-6-methylpyrimidine-2-yl) thioacetyl] mutilin (PMTM) is a new antibacterial agent with excellent antibacterial efficacy against Gram positive bacteria. For further developing PMTM as a potential drug against MRSA infections, the in vitro antibacterial efficacy and preclinical safety were explored in this study.

Covalent Organic Framework-Based Theranostic Platforms for Restricting H1N1 Influenza Virus Infection.

Background: Influenza A (H1N1) virus is a highly contagious respiratory disease that causes severe illness and death. Vaccines and antiviral drugs are limited by viral variation and drug resistance, so developing efficient integrated theranostic options appears significant in anti-influenza virus infection.

Methods: In this study, we designed and fabricated covalent organic framework (COF) based theranostic platforms (T705@DATA-COF-Pro), which was composed of an RNA polymerase inhibitor (favipiravir, T705), the carboxyl-enriched COF (DATA-COF) nano-carrier and Cy3-labeled single DNA (ssDNA) probe.

Nanopore sequencing demonstrates the roles of spermatozoal DNA N6-methyladenine in mediating transgenerational lipid metabolism disorder induced by excessive folate consumpton.

Increased consumption of folic acid is prevalent due to its beneficial effects, but growing evidence emphasizes the side effects pointing to excessive dietary folate intake. The effects of excessive paternal folic acid consumption on offspring and its transgenerational inheritance mechanism have not been elucidated. We hypothesize that excessive folic acid consumption will alter sperm DNA N6-methyladenine (6mA) and 5-methylcytosine (5mC) methylation and heritably influence offspring metabolic homeostasis.