A Novel Glycosylated Ferulic Acid Conjugate: Synthesis, Antioxidative Neuroprotection Activities In Vitro, and Alleviation of Cerebral Ischemia-Reperfusion Injury (CIRI) In Vivo.

Antioxidative neuroprotection is effective at preventing ischemic stroke (IS). Ferulic acid (FA) offers benefits in the treatment of many diseases, mostly due to its antioxidant activities. In this study, a glycosylated ferulic acid conjugate (FA-Glu), with 1,2,3-triazole as a linker and bioisostere between glucose at the C6 position and FA at the C4 position, was designed and synthesized.

Quality Evolution of Turpan Dry Red Wine During Aging Under Controlled Micro-Oxygenation.

During the aging process, oxygen regulation was applied to Cabernet Sauvignon dry red wine (CSDRW) from the Turpan region. Through physicochemical analysis, headspace solid-phase microextraction and gas chromatography-mass spectrometry (HS-SPME-GC-MS), combined with differential significance analysis and sensory evaluation, this study analyzed the effects of different oxygen concentrations on the quality of Cabernet Sauvignon dry red wine from Turpan. The results indicated that oxygen regulation facilitated the polymerization of phenolic substances, enhanced color intensity, and reduced the content of total sulfur and anthocyanins.

Cross-food-cultural study of metabolite differences in Asian spicy fermented pastes using combined CIL-LC-MS and GC-MS analysis.

Food culture, shaped by regional environments, ingredients, and fermentation systems, significantly influences the metabolites of fermented foods. Gochujang (GCJ) from Korea and Pixian-Douban (PXDB) from China are widely consumed spicy fermented pastes made from chili peppers and legumes, distinguishing them from hot sauces. However, how fermentation traditions lead to distinct metabolite profiles has not been systematically investigated.

Versatile potential of dielectric barrier discharge cold plasma in alleviating texture softening of pickled radishes during package storage by tuning microstructure, targeting cell-invading yeast and inactivating pectin-degrading enzyme.

The objective of the present study was to investigate the effect of dielectric barrier discharge cold plasma (DBD-CP) on texture, microstructure, physicochemical properties, pectin degradation, and microbial composition of pickled radishes during storage. Results showed that, when compared with control (without treatment) and blanching, DBD-CP achieved the 10.20 %-108.

Engineered Halomonas sp. Y3 enables highly efficient upcycling of poly(ethylene terephthalate) to polyhydroxyalkanoates.

The global plastic crisis demands innovative solutions for recycling polyethylene terephthalate (PET), a chemically stable polymer constituting 23 % of annual plastic waste. This study presents a significant advance in PET upcycling using engineered Halomonas sp. Y3, a halophilic bacterium uniquely suited for industrial bioprocessing.

Yeast β-glucan ameliorated Salmonella-induced gut impairment in broiler chickens by modulating gut microbiome.

Yeast β-glucan (YG) was reported to control Salmonella infection in poultry. Gut microbiota plays an important role in regulating immune functions and intestinal health. However, it is still unclear whether YG protects chickens from Salmonella infection by regulating gut microbiota.

In vitro simulated digestion and fermentation characteristics of pumpkin polysaccharides and their effects on the human gut microbiota.

Although pumpkin polysaccharides have numerous health benefits, limited studies have investigated the impact of pumpkin polysaccharide digestion and fermentation characteristics on its biological activity. Herein, pumpkin polysaccharides (PPs) were subjected to simulated saliva-gastric-small intestinal digestion, followed by in vitro fermentation using healthy human faecal microbiota. Results indicate that PPs, acidic heteropolysaccharide with a molecular weight of 2.

Construction of new thermostable MtLPMO9V in synergism with cellulases for efficient lignocellulosic hydrolysis.

Lytic polysaccharide monooxygenases (LPMOs) can promote cellulose hydrolysis by disrupting its crystalline zone. This study focused on an uncharacterized thermophilic Myceliophthora thermophila LPMO (MtLPMO9V) in synergism with cellulases for efficient ligocellulosic hydrolysis. After MtLPMO9V was successfully expressed in P.

Identification and Expression Analysis of Polyphenol Oxidase Gene Family Members in Response to Wound Stress in Lettuce ( L.).

Mechanical injury to lettuce often leads to enzymatic browning caused by polyphenol oxidase (PPO), significantly impairing its sensory quality and processing suitability. In this study, the gene family was comprehensively identified and characterized using bioinformatics methods, including gene and protein structure, codon usage bias, phylogenetic relationships, and gene expression in response to wound stress. Further analysis of the relationship between expression profile and browning was performed.

Chemical Isotope Labeling Liquid Chromatograph-Mass Spectrometer: A Powerful Tool for Analyzing Non-Volatile Organic Acids in Baijiu.

Non-volatile organic acids (NVOAs) are essential to the flavor profile of Baijiu. However, the low levels and diversity of NVOAs in Baijiu make their isolation, annotation, and quantification challenging. In this study, a well-established pipeline combining chemical derivatization, isotope labeling, and high-resolution mass spectrometry with a three-tier annotation process was used to quantify NVOAs in three typical flavor types of Baijiu with high coverage and confidence.

Enhancement of the catalytic activity of thermostable Endo-1,4-β-glucanase B (TnCelB) from Thermotoga neapolitana by error-prone PCR.

Endo-1,4-β-glucanase plays a crucial role in converting cellulose from lignocellulosic biomass into fermentable sugars for biofuel production. However, its commercial utility is hindered by poor catalytic performance under extreme conditions. This study enhanced the catalytic activity of the endo-1,4-β-glucanase (TnCelB) from Thermotoga neapolitana through error-prone PCR directed evolution.

Intermolecular interactions influenced the gelation and texture improvement of sturgeon surimi gels by walnut protein isolates.

Intermolecular interaction is a key factor in the fortification of surimi gels by plant protein addition. Here, the effects of different intermolecular interactions, such as ionic, covalent and non-covalent interactions, on gel structure, gelation strength and water-holding properties were investigated, using sturgeon surimi fortified by three walnut isolates, including walnut meal (WM), protein isolate (WPI) and peptide (WP), as representatives. Quantitative creep-recovery analysis and soluble protein assay demonstrated that secondary bonds, mainly hydrophobic interaction and hydrogen bond, possibly played a dominant role in walnut protein-fortified surimi gels.

Response of alcohol fermentation strains, mixed fermentation and extremozymes interactions on wine flavor.

Winemaking, one of the ancient technologies, is simply the process of converting sugar into alcohol through a complex biochemical reaction. The process of winemaking involves a complex of enological technique that faces a host of challenges in a winery including, inconsistent quality due to chemical and microbiological instability, limited sensory flavor profiles, and concerns met with changing micro-environmental conditions. Fermentation is a metabolic process where the chemical composition of an organic substrate is fragmented via the cellular enzymes under anaerobic conditions.

Revealing differences in flavor compounds during plum wine fermentation using single and mixed yeast strains through metabolomic analysis.

Mixed fermentation can enhance the flavor and aroma of fruit wine, but the mechanisms driving this enhancement remain unclear. This study used non-targeted metabolomics to analyze the effects of mixed versus single fermentation on plum wine flavor. The results showed that compared with single fermentation, mixed fermentation reduced ethanol content and the ability to consume reducing sugars.

Structure and metabolic function of spatiotemporal pit mud microbiome.

Background: Pit mud (PM) hosts diverse microbial communities, which serve as a medium to impart flavor and quality to Baijiu and exhibit long-term tolerance to ethanol and acids, resulting in a unique ecosystem. However, the ecology and metabolic functions of PM remain poorly understood, as many taxa in PM represent largely novel lineages. In this study, we used a combination of metagenomic analysis and chemical derivatization LC-MS analysis to provide a comprehensive overview of microbial community structure, metabolic function, phylogeny, horizontal gene transfer, and the relationship with carboxyl compounds in spatiotemporal PM samples.

Structural Characterization of Water-Soluble Pectin from the Fruit of and Its Protective Effects against DSS-Induced Colitis in Mice.

Polysaccharides from L. were investigated for their structural characterization and anti-inflammatory activity. Four low polymer dispersity index (PDI) subfractions were obtained: DRP-1 (153.

Identification and mechanistic study of piceatannol as a natural xanthine oxidase inhibitor.

Natural Xanthine oxidase (XOD) inhibitors represent promising therapeutic agents for hyperuricemia (HUA) treatment due to their potent efficacy and favorable safety profiles. This study involved the construction of a comprehensive database of 315 XOD inhibitors and development of 28 machine learning-based QSAR models. The ChemoPy light gradient boosting machine model exhibited the best performance (AUC = 0.

The Effects of Interventions with Glucosinolates and Their Metabolites in Cruciferous Vegetables on Inflammatory Bowel Disease: A Review.

Inflammatory bowel disease (IBD) is a chronic inflammatory disorder of the gastrointestinal tract which affects millions of individuals worldwide. Despite advancements in treatment options, there is increasing interest in exploring natural interventions with minimal side effects. Cruciferous vegetables, such as broccoli, cabbage, and radishes, contain bioactive compounds known as glucosinolates (GLSs), which have shown promising effects in alleviating IBD symptoms.

Mechanistic Insights into the Antioxidant Potential of Sugarcane Vinegar Polyphenols: A Combined Approach of DPPH-UPLC-MS, Network Pharmacology and Molecular Docking.

This study investigated the antioxidant potential of sugarcane vinegar, an emerging functional food, by analyzing its polyphenols and underlying molecular mechanisms that intervene in oxidative stress. Using a 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) assay combined with UPLC-MS analysis, six key polyphenols were identified: chlorogenic acid, caffeic acid, ferulic acid, luteolin, protocatechuic acid, and syringic acid. These compounds showed a positive correlation with antioxidant capacity.

In-depth recognition of mixed surfactants maintaining the enzymatic activity of cellulases through stabilization of their spatial structures.

Mixed surfactants improve the enzymatic hydrolysis of lignocellulosic substrates by enhancing cellulase stability against heat, pH, shear, and air-liquid interface stress. Under conditions of multiple factorial stresses (50 °C, pH 4.8, 180 rpm, and 15.

Preparation and characterization of κ-carrageenan/dextran films blended with nano-ZnO and anthocyanin for intelligent food packaging.

The κ-carrageenan/microbial-originated dextran-based multifunctional intelligent packaging films, integrated with natural anthocyanins as a colorant and ZnO as an antibacterial agent, were successfully developed using a casting method. Their applicability and functionality were systematically assessed through various analytical techniques. The addition of dextran, anthocyanins, and ZnO in the films resulted in an increased tensile strength (from 13.

Photo-responsive Cu-tannic acid nanoparticle-mediated antibacterial film for efficient preservation of strawberries.

The existing films used for fruit preservation suffer from insufficient preservation abilities. This study introduces Cu-tannic acid (Cu-TA) nanoparticles, synthesized from tannic acid (TA) and Cu, to enhance food packaging properties. Integrated into a chitosan-gelatin (CG) matrix, the resultant Cu-TA nanocomposite films exhibit superior antibacterial efficacy and killing rates of Escherichia coli and Staphylococcus aureus more than 99 %, and double the shelf life of strawberries, underscoring the exceptional freshness preservation capabilities of film.

Identification of novel natural anti-browning agents based on phenotypic and metabolites differences in potato cultivars.

This study aimed to elucidate the changes of browning-related metabolite in fresh-cut potato and to identify anti-browning agents. Metabolomics and weighted correlation network analysis (WGCNA) were used to identify metabolites and correlate them with potato browning traits. A total of 79 browning trait-positive-related metabolites and 19 browning trait-negative-related metabolites were obtained from four key modules via WGCNA.