Applying natural product repurposing strategy to identify baicalein as novel caseinolytic protease P inhibitor and its application in the treatment of rice bacterial diseases.

Plant diseases caused by bacteria affect the yield of crop, greatly reduce the quality of food, and thus posing a great threat to food safety. To fill the gap that no report about ClpP inhibitor is applied in agri-food production field, engineering natural-product repurposing strategy, 55 of natural products were screened using the combination of ClpP inhibitors of Xanthomonas oryzae pv. oryzae (Xoo) screening assay and anti-Xoo activity experiment.

Occurrence, spatial distribution, and risk assessment of per- and polyfluoroalkyl substances in soil and groundwater of a petrochemical industrial park in China.

Per- and polyfluoroalkyl substances (PFAS) are extensively used in the petrochemical industry and pose considerable risks to the environment. However, systematic research on PFAS contamination in petrochemical industrial parks remains limited. This study focused on the occurrence, spatial distribution, and sources of 20 typical PFAS in soil (n = 19) and groundwater (n = 13) samples from a petrochemical industrial park in China.

Unraveling ozone formation sensitivity to ambient VOCs and NOx at urban and mountain sites in a typical city of eastern China.

Ozone (O) is a primary pollutant affecting air quality in China, whose formation rate was non-linearly based on volatile organic compounds (VOCs) and nitrogen oxides (NOx). A comprehensive understanding of the key drivers governing O formation and its sensitivity to precursor variations presents a persistent research challenge, stemming from the complex interplay of underlying photochemistry, meteorology, and topography. To address this knowledge gap, we conducted synchronous measurements of O and its precursors at both an urban (JPU) and a mountain (LM) site in a typical city in eastern China, enabling concurrent evaluation of O formation sensitivity and diagnostic analysis of its underlying mechanisms.

Decahedron-derived five-fold twin noble metal nanocrystals: Solution-phase synthesis, growth mechanism, and applications.

Five-fold twinned (FFT) noble metal nanocrystals have attracted considerable interest in nanoscience due to their unique structures. This review gives a comprehensive analysis on four common types of FFT noble metal NCs (Au, Ag, Pd, and Pt) synthesized in solution, covering both monometallic structures (e.g.

Albumin Binding Enhances the Lysosomal β-Galactosidase Sensitivity of Fluorogenic Probes Characteristic of Intramolecular Charge Transfer.

Glycosidases generally function in specific organelles to hydrolyze glycoconjugates. Thus, the in situ visualization of glycosidase activities in an organelle-targeted manner can help to better delineate their biological functions. Lysosomal β-galactosidase (β-Gal) is reported to be a biomarker for ovarian cancer and cellular senescence.

[Advances in the development of analysis techniques for organophosphate diesters in water].

Organophosphate triesters （tri-OPEs） are synthetic phosphate derivatives that are primarily used as flame retardants and plasticizers. Tri-OPEs have become significant aquatic contaminants owing to their large production volumes and wide range of applications. Organophosphate diesters （di-OPEs） are closely related to tri-OPEs.

Ultrabroadband Photosensitivity and Frequency-Mixing in Anisotropic Weyl Semimetal NbNiTe.

Broadband anisotropic photodetectors show great promise for polarization-sensitive imaging and multispectral optoelectronic systems yet face critical challenges in material anisotropy modulation and broadband sensitivity. Weyl semimetals exhibit giant optical anisotropy and tunable heterojunction band alignment, enabling high-performance anisotropic photodetection. Herein, ultrabroadband PDs based on the NbNiTe (niobium nickel telluride), enabled by antenna integration and heterostructure engineering, achieve high sensitivity from visible to Terahertz (THz).

AMPK maintains the activation of hepatic stellate cells through mitophagy-induced metabolic reprogramming.

The activation of hepatic stellate cells (HSCs), characterized by transdifferentiation from a quiescent state to a fibrogenic phenotype, is a core process of liver fibrosis. The metabolic reprogramming of HSCs plays a major role in this process to meet the high energy demands of myofibroblastic HSCs with multiple functions, such as extracellular matrix synthesis, migration, and proliferation. AMP-activated protein kinase (AMPK) is a gatekeeper of intracellular energy homeostasis, but its role in the activation of HSCs and the progression of liver fibrosis remains unclear.

Augmentation of PRDX1-DOK3 interaction alleviates rheumatoid arthritis progression by suppressing plasma cell differentiation.

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by persistent inflammation and joint damage, accompanied by the accumulation of plasma cells, which contributes to its pathogenesis. Understanding the genetic alterations occurring during plasma cell differentiation in RA can deepen our comprehension of its pathogenesis and guide the development of targeted therapeutic interventions. Here, our study elucidates the intricate molecular mechanisms underlying plasma cell differentiation by demonstrating that PRDX1 interacts with DOK3 and modulates its degradation by the autophagy-lysosome pathway.

Optimizing blood-brain barrier permeability in KRAS inhibitors: A structure-constrained molecular generation approach.

Kirsten rat sarcoma viral oncogene homolog (KRAS) protein inhibitors are a promising class of therapeutics, but research on molecules that effectively penetrate the blood-brain barrier (BBB) remains limited, which is crucial for treating central nervous system (CNS) malignancies. Although molecular generation models have recently advanced drug discovery, they often overlook the complexity of biological and chemical factors, leaving room for improvement. In this study, we present a structure-constrained molecular generation workflow designed to optimize lead compounds for both drug efficacy and drug absorption properties.

Multiomics Insights into the Mechanism and Enhanced Efficacy of Tumor Treating Fields (TTFields) Therapy in Glioblastoma.

Glioma is an aggressive brain tumor that requires challenging treatments. Tumor Treating Fields (TTFields), an FDA-approved therapy for glioblastoma (GBM), pleural mesothelioma, and platinum-refractory metastatic nonsmall cell lung cancer (in combination with PD-1/PD-L1 inhibitors or docetaxel), employs specific frequency electric fields to disrupt cell division and enhance treatment efficacy. However, their molecular mechanisms remain unclear.

Aptamer-based targeted drug delivery and disease therapy in preclinical and clinical applications.

The advent of precision medicine has created an urgent need for advanced drug-targeting strategies and refined drug delivery systems. Aptamers, characterized by their exceptional affinity and specificity, low molecular weight, negligible immunogenicity, remarkable stability, cost-effectiveness, and structural versatility, are emerging as promising candidates in targeted therapeutics, both in preclinical research and clinical applications. This review provides a comprehensive analysis of the latest advancements in aptamer-based therapeutic strategies, encompassing three key application domains: direct therapeutic agents, targeted ligand engineering, and controlled drug release.

Deciphering chromatin domain, domain community and chromunity for 3D genome maps with Mactop.

Advanced high-throughput chromosome conformation capture techniques, like Hi-C, reveal genome organization into structural units like topologically associating domains (TADs), which are crucial in gene expression regulation. While accurately identifying TADs is vital, distinguishing different types of TAD boundaries and TAD categories remains a significant challenge in genomic research. We develop a Markov clustering-based tool, Mactop, to accurately identify TADs and provide biologically important classifications of TADs and their boundaries.

Synthetic Membranes for Aqueous Organic Redox Flow Batteries (AORFBs): towards High Conductive and Selective Ion Channels.

Aqueous organic redox flow batteries (AORFBs) have emerged as one of the most promising electrochemical technologies for large-scale energy storage due to their use of water-based electrolytes, offering safety and cost advantages over organic solvent-based systems. AORFBs utilize organic molecules derived from earth-abundant elements, enabling tunable properties such as solubility, stability, and redox potential at the molecular level. These features enable improvements in energy and power densities, operational lifetimes, and efficiency metrics in the battery system.

Probe-Encapsulated and Cell-Membrane-Anchored DNA Nanotoolboxes Enable Imaging of Extracellular Biochemical Components in Complex Biological Media.

Cell-membrane-anchored DNA probes are powerful tools for membrane-localized biosensing, bioregulation, and biotherapy studies. However, the poor biostability, membrane-anchoring stability, and effectiveness of membrane-anchored DNA probes in complex physiological environments have seriously hindered their widespread applications. In this work, probe-encapsulated DNA nanotoolboxes were developed by immobilizing DNA probes into DNA cuboids to improve the performance of cell-membrane-anchored DNA probes in complex biological media.

Post-PKS Tailoring of Phoslactomycins Involving Two Cytochrome P450s.

Combining and approaches, we elucidated the phoslactomycin post-PKS tailoring pathway in . Gene inactivation of - revealed seven phoslactomycin derivatives (four novel). Two cytochrome P450s were identified: PnT3 (mediating dual-site, multistep C-8/C-25 oxidation) and PnT7 (catalyzing C-18 hydroxylation).

Saturated Alcohols Electrocatalytic Oxidations on Ni-Co Bimetal Oxide Featuring Balanced B- and L-Acidic Active Sites.

Investigating structural and hydroxyl group effects in electrooxidation of alcohols to value-added products by solid-acid electrocatalysts is essential for upgrading biomass alcohols. Herein, we report efficient electrocatalytic oxidations of saturated alcohols (C-C) to selectively form formate using NiCo hydroxide (NiCo-OH) derived NiCoO solid-acid electrocatalysts with balanced Lewis acid (LASs) and Brønsted acid sites (BASs). Thermal treatment transforms BASs-rich (89.

Recent advances in small molecules targeting the METTL3.

-methyladenosine (mA) is the most prevalent internal modification of eukaryotic mRNA, playing a crucial role in the regulation of gene expression. Methyltransferase-like 3 (METTL3), a key catalytic component of the mA methyltransferase complex, is primarily responsible for the deposition of mA on target RNA. Recent studies have revealed that METTL3 contributes to diverse pathological processes, particularly tumorigenesis, through both mA-dependent and independent mechanisms.

Epigenetic Programming of Estrogen Receptor in Adipocytes by High Fat Diet Regulates Obesity-Induced Inflammation.

Adipose inflammation plays a key role in obesity-induced metabolic abnormalities. Epigenetic regulation, including DNA methylation, is a molecular link between environmental factors and complex diseases. Here we found that high fat diet (HFD) feeding induced a dynamic change of DNA methylome in mouse white adipose tissue (WAT) analyzed by reduced representative bisulfite sequencing.

High-sensitivity broadband terahertz detection enabled by synergistic effects in an antenna-integrated TaCoTe/graphene heterostructure.

A van der Waals (vdW) heterostructure based on the semimetals TaCoTe and graphene is fabricated with an integrated fan-shaped conductive antenna and has been investigated for terahertz (THz) detection at room temperature. Assisted by the fan-shaped conductive antenna, the vdW detector exhibits high sensitivity and broadband detection from 0.02 to 0.

Colloidal InSb Quantum Dots Mid-Wave Infrared Photoconductive Detectors via One-Step Strong Acid Surface Treatment Strategy.

Colloidal InSb quantum dots (QDs) hold significant promise in infrared photodetection. However, the current InSb QDs suffer from poor carrier mobility and limited spectral response (<1.8 μm) due to complex surface structure and high sensitivity to hydrolysis and oxidation.

Interactions of Aqueous Microdroplets and Mineral Particles Drive Fluorine-First Perfluoroalkyl Mineralization.

Anthropogenic perfluoroalkyl and polyfluoroalkyl substances (PFAS) are pervasive contaminants subject to increasingly stringent regulatory thresholds in water resources. Current nonthermal defluorination strategies face critical limitations, including incomplete mineralization, yielding persistent short-chain PFAS byproducts, and residual fluoride ions, thereby hindering compliance with water quality standards. Herein, we demonstrate that wollastonite-bearing microdroplets prioritize defluorination over C-C scission in perfluoroalkyl chains through liquid-solid-gas triple-phase contact electrification.