[Advances in the development of analysis techniques for organophosphate diesters in water].

Organophosphate triesters （tri-OPEs） are synthetic phosphate derivatives that are primarily used as flame retardants and plasticizers. Tri-OPEs have become significant aquatic contaminants owing to their large production volumes and wide range of applications. Organophosphate diesters （di-OPEs） are closely related to tri-OPEs.

Synergistic Effect of Guanidinium and Tertiary Amine Groups on Boosting Gene Delivery.

The lack of safe and efficient delivery vectors remains a major bottleneck in nucleic acid therapeutics, limiting clinical translation. In this study, by incorporating the synergistic effects of guanidinium and tertiary amine functional groups, a new versatile branched poly-(β-amino ester) (PAE)-based delivery system was designed and developed, resulting in enhanced nucleic acid (DNA/mRNA) delivery. The synergistic interactions between these functional groups were systematically analyzed using 2D-NMR, AFM, TEM, and photophysical characterization.

Seven Traditional Chinese Medicine external treatments combined with rehabilitation training on the functional recovery of limbs in patients with cerebral hemorrhage: a network Meta-analysis.

Objective: To evaluate the effectiveness of the combined use of 7 commonly used Traditional Chinese Medicine external treatment methods and rehabilitation training in improving limb function in patients with cerebral hemorrhage through a network Meta-analysis.

Methods: A computer-based search was conducted in 8 databases, including China National Knowledge Infrastructure Database, Wanfang Database, China Science and Technology Journal Database, PubMed, Cochrane Library, Web of Science, Scopus, and Embase, from their inception until February 19, 2023. Randomized controlled trials (RCTs) investigating the effectiveness of the combined use of 7 commonly used Traditional Chinese Medicine external treatment methods and rehabilitation training in improving limb function in patients with cerebral hemorrhage were included.

RNA transcripts regulate G-quadruplex landscapes through G-loop formation.

G-quadruplexes (G4s) are prevalent DNA structures that regulate transcription but also threaten genome stability. How G4 dynamics are controlled remains poorly understood. Here, we report that RNA transcripts govern G4 landscapes through coordinated G-loop assembly and disassembly.

Epitaxially Grown Lattice-Coherent Surface Enabling Superior Mechanical Integrity for High-Voltage LiCoO Cathode.

The growing demand for high-energy-density cathode is pushing LiCoO towards 4.6 V operation. However, the structural and interfacial instability of high-voltage LiCoO is exacerbated when the charging cut-off voltage exceeds 4.

Beyond Flory's principle: Cyclization and unequal reactivity in step-growth linear polymerization.

Step-growth polymerization (SGP) products are ubiquitous, but the theoretical understanding of SGP reactions has stagnated since the introduction of Flory's classical theory. Flory's model based on two key assumptions-equal reactivity of functional groups and the absence of cyclization-falls short in guiding real-world polymerization processes. In this work, we extend Flory's model by accounting for individual reaction probabilities and cyclization tendencies, making it applicable to real SGP situations.

The association between body roundness index and sarcopenia in older adults: a population-based study.

Background: Sarcopenia, defined by the gradual decline in skeletal muscle mass and functionality, is a common disorder in the aging population and is linked to an elevated risk of falls and osteoporotic fractures. The contemporary diagnosis of sarcopenia depends on intricate and expensive techniques, such as computed tomography (CT) scans or dual-energy X-ray absorptiometry (DXA), which hinder the timely prevention of sarcopenia.

Objective: This study seeks to explore the association between the Body Roundness Index (BRI) and sarcopenia in the older adult cohort, utilizing data from the National Health and Nutrition Examination Survey (NHANES) in the United States.

Visit probability and accessibility within space-time prism of activity program.

Space-time prism (STP) is widely applied to measure individuals' ability to reach opportunities given the resource limitations. The majority of STP models treat prism-based accessibility as binary measures, wherein all locations within a prism are assumed equally accessible while others are deemed inaccessible. Although a few STP models examined heterogeneous interiors by modeling visit probabilities within the STPs, they primarily focused on trip-level analysis and did not explore the application for accessibility measurement.

Water Soluble Thermoresponsive Single-Chain Cyclized/Knotted Polymers from Intramolecular Cyclization Dominated Homopolymerization of PEG Diacrylate.

A series of water-soluble, PEG-based single-chain cyclized/knotted polymers are successfully synthesized through the homopolymerization of poly(ethylene glycol) diacrylate (PEGDA) (M  = 575 and 700 g mol, respectively) via enhanced intramolecular cyclization. The homopolymerization of the diacrylate macromers first proceeds through linear propagation, followed by self-cyclization. Under high monomer-to-initiator ratios (e.

Highly branched poly β-amino ester/CpG-depleted plasmid nanoparticles for non-viral gene therapy in lung cystic fibrosis disease.

Lung cystic fibrosis (CF) is a lethal inherited disease caused by mutations in the CF transmembrane conductance regulator () gene, leading to a dysfunctional CFTR protein. Gene therapy offers promise for the treatment of lung CF. However, the development and clinical application of CF gene therapy have long been hampered by the absence of safe and highly efficient delivery vectors.

Roving histological imaging for navigation-confirmed glioma negative margin by handheld endomicroscopy: a parallel controlled study.

Introduction: Roving histopathological imaging in navigation-confirmed tumor negative margin has not been achieved during neurosurgery procedures. This study aims to explore the diagnostic values of a novel intraoperative handheld endomicroscopy DiveScope (DS) for ex vivo glioma diagnosis and in vivo roving scan for navigation-confirmed glioma negative margin and evaluate its safety.

Materials And Methods: This prospective pilot study was conducted at Huashan Hospital, Fudan University from October 2021 to October 2023, and enrolled patients scheduled for glioma resection surgery.

Bone marrow niches orchestrate stem-cell hierarchy and immune tolerance.

Stem cells reside in specialized microenvironments, termed niches, at several different locations in tissues. The differential functions of heterogeneous stem cells and niches are important given the increasing clinical applications of stem-cell transplantation and immunotherapy. Whether hierarchical structures among stem cells at distinct niches exist and further control aspects of immune tolerance is unknown.

Emotional salience network involved in constructing two-dimensional fear space in humans.

Fear learning is pivotal for organismal survival, ensuring the ability to avoid potential threats through learning based on experiencing minimal fear information. In reality, fear learning requires to form a structured representation of fear experiences from multiple dimensions in order to support flexible use in ever-changing environment. Yet, the underlying neural mechanisms of constructing dimensional fear space remain elusive.

Efficacy and safety of traditional Chinese medicine combined with azithromycin sequential therapy for pneumonia among children: a meta-analysis of randomized controlled trials.

Background: Traditional Chinese medicine (TCM) is used to treat pneumonia (MP) in children with favorable treatment outcome in China. In the present study, we evaluated the clinical efficacy of TCM combined with azithromycin (AZM) for the treatment of MP among children, providing high evidence-based reference for clinical treatment.

Method: We retrieved eligible randomized controlled trials (RCTs) from CQVIP, CNKI, WanFang, NSTL, PubMed, Embase, and Embase databases from January 2000 to November 2023.

Visceral fat area and subcutaneous fat area as measures of body composition in soft tissue sarcoma.

Background And Objectives: Soft tissue sarcomas (STS) are a heterogenous group of malignancies of mesenchymal origin. Given recent data linking obesity as well as the pattern of fat distribution with cancer outcomes, we sought to investigate the association of visceral fat area (VFA) and subcutaneous fat area (SFA) with oncologic outcomes in patients with STS undergoing surgery.

Methods: We analyzed data from 88 patients with STS diagnosed from 2008 to 2022.

Characterization of the Biological Variability of the Angiome Biomarkers over Time in Healthy Participants.

Background: Biomarker analyses are an integral part of cancer research. Despite the intense efforts to identify and characterize biomarkers in patients with cancer, little is known regarding the natural variation of biomarkers in healthy populations. Here we conducted a clinical study to evaluate the natural variability of biomarkers over time in healthy participants.

Multiplexed chromatin immunoprecipitation sequencing for quantitative study of histone modifications and chromatin factors.

ChIP-seq is a widely used technique for studying histone post-translational modifications and DNA-binding proteins. DNA fragments associated with a specific protein or histone modification epitope are captured by using antibodies, sequenced and mapped to a reference genome. Albeit versatile and popular, performing many parallel ChIP-seq experiments to compare different conditions, replicates and epitopes is laborious, is prone to experimental variation and does not allow quantitative comparisons unless adequate spike-in chromatin is included.

Engineered Composite Interfacial Electric Field Boosts Piezocatalysis of Perovskite Ferroelectrics.

Reducing the level of annihilation of electrons and holes is considered to be a feasible strategy to promote piezocatalytic activities. But this strategy is only achieved through cumbersome sample preparation technologies, hindering its practical applications. Herein, we introduce a simple and efficient technique, the conventional solid-state method, to engineer a composite interfacial electric field to solve this problem, and validate it in a composite piezocatalysis composed of potassium sodium niobate ((K, Na)NbO, KNN) and multiwalled carbon nanotubes (MWCNTs).

Fast one-step acrylate functionalization of hyaluronic acid Williamson ether synthesis.

The synthetic route presented for acrylate-modified hyaluronic acid (HA-A-BEA) offers a simple and efficient process, reducing reaction time and purification steps while retaining biocompatibility. This study demonstrates the ability of HA-A-BEA to form tunable hydrogels versatile techniques suitable for biomedical applications.

The interconnection of orthographic, phonetic, and semantic skills with arithmetic fluency.

Arithmetic fluency is considered considers highly rely on language processing, encompassing essential skills. However, the independent predictive power of phonetic, semantic, or orthographic skills in relation to arithmetic fluency remains an unresolved query. This study introduces the common component hypothesis to elucidate the inconsistent findings in previous research.

Lexical tone recognition in multi-talker babbles and steady-state noise by Mandarin-speaking children with unilateral cochlear implants or bimodal hearing.

Background And Objectives: Lexical tone presents challenges to cochlear implant (CI) users especially in noise conditions. Bimodal hearing utilizes residual acoustic hearing in the contralateral side and may offer benefits for tone recognition in noise. The purpose of the present study was to evaluate tone recognition in both steady-state noise and multi-talker babbles by the prelingually-deafened, Mandarin-speaking children with unilateral CIs or bimodal hearing.

Hyaluronic Acid/Chondroitin Sulfate-Based Dynamic Thiol-Aldehyde Addition Hydrogel: An Injectable, Self-Healing, On-Demand Dissolution Wound Dressing.

Frequent removal and reapplication of wound dressings can cause mechanical disruption to the healing process and significant physical discomfort for patients. In response to this challenge, a dynamic covalent hydrogel has been developed to advance wound care strategies. This system comprises aldehyde functionalized chondroitin sulfate (CS-CHO) and thiolated hyaluronic acid (HA-SH), with the distinct ability to form in situ via thiol-aldehyde addition and dissolve on-demand via the thiol-hemithioacetal exchange reaction.

Identification of a metabolism-linked genomic signature for prognosis and immunotherapeutic efficiency in metastatic skin cutaneous melanoma.

Metastatic skin cutaneous melanoma (MSCM) is the most rapidly progressing/invasive skin-based malignancy, with median survival rates of about 12 months. It appears that metabolic disorders accelerate disease progression. However, correlations between metabolism-linked genes (MRGs) and prognosis in MSCM are unclear, and potential mechanisms explaining the correlation are unknown.