Aptamer-based targeted drug delivery and disease therapy in preclinical and clinical applications.

The advent of precision medicine has created an urgent need for advanced drug-targeting strategies and refined drug delivery systems. Aptamers, characterized by their exceptional affinity and specificity, low molecular weight, negligible immunogenicity, remarkable stability, cost-effectiveness, and structural versatility, are emerging as promising candidates in targeted therapeutics, both in preclinical research and clinical applications. This review provides a comprehensive analysis of the latest advancements in aptamer-based therapeutic strategies, encompassing three key application domains: direct therapeutic agents, targeted ligand engineering, and controlled drug release.

Analysis of Methadone-Related Poisoning Cases.

Objectives: To analyze the characteristics of methadone-related poisoning cases and provide a reference for forensic identification.

Methods: A total of 71 cases of methadone-related poisoning reported from 1998 to 2023 in China and 26 cases of methadone-related deaths reported from 2013 to 2018 in Italy were retrieved from databases including PubMed, Wanfang and CNKI. The general information, forensic pathological and toxicological characteristics were analyzed.

Precision Mapping of Direct Membrane Protein Interactions via Binding-Induced DNA Barcode Transfer Labeling.

Cell-cell communication is governed by dynamic membrane protein interactions. Precise tracking of direct protein binding and its functional outcomes across cellular engagements is essential for decoding complex biological processes. While proximity-based labeling techniques convert transient interactions into stable signals, they often fail to distinguish direct binding from nonspecific associations.

The Implementation Gap: A Qualitative Analysis of Determinants of Sexual Counseling Among Coronary Heart Disease Patients in China.

Objective: Coronary heart disease affects the quality of sexual life of patients, sexual life is very important for patients. Sexual counseling is an effective intervention to improve the quality of sexual life in patients with coronary heart disease. However, the implementation of sexual counseling is not very well.

Development of a matching framework for voluntary services provided to home-dwelling older adults by nursing faculty and students based on the Kano model.

Background: As population aging accelerates, volunteer services provide meaningful community support for older adults. However, evidence is limited on how to align their needs with the service intentions of nursing faculty and students to develop appropriate frameworks.

Methods: Data were collected through self-designed demand-supply matching questionnaires from nursing faculty and students (March 26 - April 25, 2023) and older adults (May 24 - July 7, 2023) using convenience sampling.

Anomalous Precipitation of the γ-Fe Phase in Fe-Based Nanocrystalline Alloys and Its Impact on Soft Magnetic Properties.

High-Cu-content (Cu-content > 1.3 at.%) nanocrystalline alloys exhibit wide heat-treatment windows and favorable soft magnetic properties due to the presence of pre-existing α-Fe nanocrystals.

Infectome analysis of bat kidneys from Yunnan province, China, reveals novel henipaviruses related to Hendra and Nipah viruses and prevalent bacterial and eukaryotic microbes.

Bats are natural reservoirs for a wide range of microorganisms, including many notable zoonotic pathogens. However, the composition of the infectome (i.e.

Small mammals in a biodiversity hotspot harbor viruses of emergence risk.

Metagenomic sequencing has transformed the understanding of viral diversity in wildlife and the potential threats these viruses pose to human health. Despite this progress, such sequencing studies often have lacked systematic and ecologically informed sampling, thereby likely missing many potential human pathogens and the drivers behind their ecology, evolution and emergence. We conducted an extensive search for viruses in the lungs, spleens and guts of 1688 mammals from 38 species across 428 sites in Yunnan Province, China-a hotspot for zoonoses emergence.

Developing and validation of the Sexual Fear Scale in Chinese patients with coronary heart disease.

Background: Sexual fear in patients with coronary heart disease (CHD) can negatively affect their sexual life and overall well-being. However, validated tools for assessing this fear are lacking. This study aimed to develop a sexual fear scale for patients with CHD and evaluate its psychometric properties.

Berberine inhibits metastasis of ovarian cancer by blocking lipid metabolism, alleviating aging of adipose tissue and increasing tumor infiltrating immune cells.

Extensive peritoneal metastasis and malignant ascites continue to pose substantial challenges in achieving favorable treatment outcomes for ovarian cancer. Berberine (BBR), an active component of numerous traditional Chinese herbs, has demonstrated potent anti - tumor effects across various malignancies, including ovarian cancer. In this study, we comprehensively evaluated the impact of BBR on the growth and metastasis of ovarian cancer both in vitro and in vivo.

Vitamin D and Dapagliflozin Alleviate Renal Injury and Insulin Resistance in a Diet-Induced Metabolic Syndrome Rat Model.

Metabolic syndrome, primarily driven by high-fat/high-sugar (HF/HS) diets, is closely linked to insulin resistance and renal injury, leading to serious complications such as type 2 diabetes mellitus and diabetic nephropathy. This study explored the combined effects of Vitamin D (VD) and dapagliflozin (Dap) on metabolic and renal complications in an HF/HS diet-induced rat model of metabolic syndrome. The combination therapy significantly improved insulin sensitivity, reduced fasting blood glucose levels, and alleviated renal injury markers such as blood urea nitrogen and creatinine.

Peritoneal adipose stem cell-derived extracellular vesicles mediate the regulation of ovarian cancer cell proliferation and migration through EGFR-NF-κB signaling.

Peritoneal dissemination frequently develops in patients with ovarian cancer (OC) and is associated with recurrence and metastasis. However, the cellular components and mechanisms supporting OC peritoneal metastasis are poorly understood. To elucidate these, we utilized RNA sequencing to investigate the cellular composition and function.

Advancements and Future Prospects in Ocean Wave Energy Harvesting Technology Based on Micro-Energy Technology.

Marine wave energy exhibits significant potential as a renewable resource due to its substantial energy storage capacity and high energy density. However, conventional wave power generation technologies often suffer from drawbacks such as high maintenance costs, cumbersome structures, and suboptimal conversion efficiencies, thereby limiting their potential. The wave power generation technologies based on micro-energy technology have emerged as promising new approaches in recent years, owing to their inherent advantages of cost-effectiveness, simplistic structure, and ease of manufacturing.

Sodium bicarbonate potentiates the antitumor effects of Olaparib in ovarian cancer via cGMP/PKG-mediated ROS scavenging and M1 macrophage transformation.

The high metabolic requirements of cancer cells result in excess accumulation of H in the tumor microenvironment. Therefore, the extracellular pH of solid tumors is acidic, whereas the pH of normal tissues is more alkaline. The acidic tumor environment is correlated with tumor metastasis, immune escape, and chemoresistance, but the underlying mechanisms remain elusive.

Kinetic investigation on the catalytic pyrolysis of plastic fractions of waste electrical and electronic equipment (WEEE): A mathematical deconvolution approach.

Waste electrical and electronic equipment (WEEE) has become a critical environmental problem. Catalytic pyrolysis is an ideal technique to treat and convert the plastic fraction of WEEE into chemicals and fuels. Unfortunately, research using real WEEE remains relatively limited.

Duzhong Fang ameliorates cognitive impairment of Parkinsonian mice by suppressing neuronal apoptotic pathway.

Parkinson's disease (PD) is a complex multisystem neurodegenerative disease, and cognitive impairment is a common symptom in the trajectory of PD. Duzhong Fang (DZF) consists of Eucommia ulmoides, Dendrobium, Rehmanniae Radix, and Dried Ginger. Our previous study showed that DZF improves motor deficits in mice.

A rare case of Richter transformation with breast involvement: A case report and literature review.

Richter transformation (RT) represents the development of intrusive lymphoma in individuals previously or concurrently diagnosed with chronic lymphocytic leukemia (CLL) and is characterized by lymph node enlargement. However, cases involving extra-nodal organ involvement as the first symptom are rare. There are no reports of RT with breast lesions as the first symptom.

Transcriptomic Investigation of the Virus Spectrum Carried by Midges in Border Areas of Yunnan Province.

Yunnan province in China shares its borders with three neighboring countries: Myanmar, Vietnam, and Laos. The region is characterized by a diverse climate and is known to be a suitable habitat for various arthropods, including midges which are notorious for transmitting diseases which pose significant health burdens affecting both human and animal health. A total of 431,100 midges were collected from 15 different locations in the border region of Yunnan province from 2015 to 2020.

IL-2-free tumor-infiltrating lymphocyte therapy with PD-1 blockade demonstrates potent efficacy in advanced gynecologic cancer.

Background: Tumor-infiltrating lymphocyte (TIL) therapy has been restricted by intensive lymphodepletion and high-dose intravenous interleukin-2 (IL-2) administration. To address these limitations, we conducted preclinical and clinical studies to evaluate the safety, antitumor activity, and pharmacokinetics of an innovative modified regimen in patients with advanced gynecologic cancer.

Methods: Patient-derived xenografts (PDX) were established from a local recurrent cervical cancer patient.

Distributed electrified heating for efficient hydrogen production.

This study introduces a distributed electrified heating approach that is able to innovate chemical engineering involving endothermic reactions. It enables rapid and uniform heating of gaseous reactants, facilitating efficient conversion and high product selectivity at specific equilibrium. Demonstrated in catalyst-free CH pyrolysis, this approach achieves stable production of H (530 g h L ) and carbon nanotube/fibers through 100% conversion of high-throughput CH at 1150 °C, surpassing the results obtained from many complex metal catalysts and high-temperature technologies.

Molecular epidemiological study of Scrub Typhus in residence, farm and forest habitats from Yunnan Province, China.

The number of people suffering from scrub typhus, which is not of concern, is increasing year by year, especially in Yunnan Province, China. From June 1, 2021 to August 15, 2022, a total of 505 mammalian samples were collected from farm, forest, and residential habitats with high incidence of scrub typhus in Yunnan, China, for nPCR (nested PCR) and qPCR (quantitative real-time PCR) detection of Orientia tsutsugamushi. A total of 4 orders of murine-like animals, Rodentia (87.

Metagenomic analysis of individual mosquito viromes reveals the geographical patterns and drivers of viral diversity.

Mosquito transmitted viruses are responsible for an increasing burden of human disease. Despite this, little is known about the diversity and ecology of viruses within individual mosquito hosts. Here, using a meta-transcriptomic approach, we determined the viromes of 2,438 individual mosquitoes (81 species), spanning ~4,000 km along latitudes and longitudes in China.

Bio-based anode material production for lithium-ion batteries through catalytic graphitization of biochar: the deployment of hybrid catalysts.

Producing sustainable anode materials for lithium-ion batteries (LIBs) through catalytic graphitization of renewable biomass has gained significant attention. However, the technology is in its early stages due to the bio-graphite's comparatively low electrochemical performance in LIBs. This study aims to develop a process for producing LIB anode materials using a hybrid catalyst to enhance battery performance, along with readily available market biochar as the raw material.

Family resilience, patient-reported symptoms in young stroke dyads: The effect of caregiver readiness and social support.

Aims And Objectives: To investigate empirically the direct effect and potential mechanism of family resilience on patient-reported outcomes among young stroke dyads in China.

Background: Young patients with stroke have been becoming an important public health issue. According to relevant theories and previous studies, we found that family resilience might play an important role in patient's symptoms.