Low-Energy Femtosecond Laser Lens Fragmentation Techniques for Cataract.

Femtosecond laser has become an important tool in cataract surgery due to its precision and minimally invasive nature. However, the strong scattering properties of the lens limit surgical efficiency, especially when it comes to effectively cutting highly opaque and hard cataracts with femtosecond laser. This study proposes a method for achieving optical transparency in opaque lenses through tartrazine treatment.

Enhancement of sulforaphene stability via hydroxypropyl methylcellulose-based solid dispersions: exploiting hydrophobic cavities and hydrogen bonding-mediated water sequestration.

Sulforaphene (SFE) is a bioactive isothiocyanate, known for its cancer-preventive, anti-inflammatory, and antioxidant properties. However, the application of SFE is severely limited by its poor stability. Hydroxypropyl methylcellulose (HPMC), an amphiphilic carbohydrate polymer, has potentials to enhance the stability of SFE and the loading capacity.

Eco-engineered recombinant collagen III-PEG hydrogels with curcumin synergy for accelerated skin regeneration: A green crosslinking strategy in wound therapeutics.

The development of immunologically safe collagen-based hydrogels remains challenging due to risks associated with animal-derived collagens and toxic crosslinkers. Here, we report an eco-friendly hydrogel platform integrating recombinant humanized collagen type III (PCIII) expressed in Pichia pastoris with 4-arm PEG-SS via a one-step green crosslinking strategy. The engineered PEG-PCIII hydrogels exhibit tunable mechanics, enzymatic degradability, and rapid self-gelation through amine-NHS ester conjugation, while curcumin loading endows it with adjustable antioxidant activity.

Lanthanide Core-Satellite Nanoprobes with Dually Activatable Magnetic Resonance and Second Near-Infrared Fluorescence Signals for Perioperative Image-Guided Cancer Surgery.

Image-guided surgery plays a critical role in improving the cancer patient prognosis. However, current clinical probes are often single-modal with "always-on" signals, failing to provide complementary and precise guidance across all perioperative phases. To tackle this hurdle, we develop a biomarker-activatable, multimodal nanoprobe - - based on redox-mediated manganese valence switching for tumor-specific, perioperative image-guided surgery.

Cowpea Lipid Transfer Protein LTP1 Mediates Plant Resistance to .

Plant lipid transfer proteins (LTPs), belonging to pathogenesis-related protein 14 family, participate in plant immune response to biotic stress. LTP1 from was previously shown to be able to suppress infection by cowpea mosaic virus and soybean mosaic virus. However, whether cowpea LTP1 participates in the plant resistance to other plant pathogens remains unclear.

A High-Quality Chromosome-Level Genome Assembly and Comparative Analyses Provide Insights into the Adaptation of (Fabricius, 1794) (Diptera: Calliphoridae).

, as one of the common blowflies, displays biological characteristics, such as ovoviviparity and carrion-feeding adaptation. Thus, this species is generally considered of significant ecological, medical, and forensic importance. However, without a high-quality pseudo-chromosome genome for , elucidating its evolutionary trajectory proved difficult.

Composite nano copper carrier combining cuproptosis and photodynamic therapy for spatiotemporal synergistic anti-tumor therapy.

Cuproptosis is a copper-dependent programmed cell death triggered by mitochondrial dysfunction, which offers significant anti-tumor potential but requires tumor-specific copper delivery to avoid systemic toxicity. Here, we developed a synergistic nanoplatform (CuO@SiO-Ce6, CSC) integrating cuproptosis induction with photodynamic therapy (PDT). A cuprous oxide (CuO) core was encapsulated in silicon dioxide and covalently linked to the photosensitizer Ce6.

Cancer Cell-Secreted miR-33a Reduces Stress Granule Formation by Targeting Polyamine Metabolism in Stroma to Promote Tumourigenesis.

Tumour progression depends on the bidirectional interactions between cancer and stroma in the heterogeneous tumour microenvironment (TME) partially through extracellular vesicles (EVs). However, the secretary mechanism and biological effect of cancer cell derived EVs on tumour survival under starvation is poorly defined. Here, we identify cancer cells selectively secrete miR-33a with the assistance of aconitase 1 (ACO1), an iron-responsive RNA binding protein, under glucose starvation and lower iron level, which affiliates the binding capability of miR-33a and ACO1.

Butylparaben-Loaded Aged Polystyrene Nanoplastics Amplify Its Toxicity in via Quorum Sensing Suppression and Enhanced Microcystin-LR Release.

Aged nanoplastics are emerging pollutants in aquatic environments, but the effects of their loaded pollutants on cyanobacteria are still poorly understood. This study evaluated the adsorption of butylparaben (BP) by pristine (PS) and aged polystyrene nanoplastics (APS) and prepared BP-loaded aged PS to analyze their effects on . The results showed that APS had stronger BP adsorption and translocation capacity, with APS increasing BP adsorption by 4.

Yeasts in traditional Baijiu fermentation: diversity, functions, microbial interactions and applications.

Baijiu is a traditional distilled liquor unique to China. Its distinctive flavor is shaped by the synergistic activity of complex microbial communities, among which yeasts play a central role in sugar metabolism, ethanol fermentation, and aroma synthesis. In recent years, the advancement of isolation and cultivation techniques, high-throughput sequencing, metagenomics, and multi-omics technologies has deepened our understanding of yeast community compositions, succession patterns, and functional characteristics during Baijiu brewing.

Decoding sugarcane smut: the role of effector SsEF83 in fungal virulence and plant interaction.

Sugarcane smut, caused by the fungus , severely affects global sugarcane production. Effector proteins are crucial for fungal invasion and virulence in plants. Nevertheless, few studies have identified effector proteins in and demonstrated their functions in smut progression.

Selection of geographical populations suitable for artificial breeding of the Northeast China Brown Frog (Rana dybowskii).

Amphibians, as a group greatly disturbed by human activities, are at increased risk of extinction. Rana dybowskii is an anuran species with both ecological and economic significance. Due to environmental changes and human overexploitation, it has been classified as Near-Threatened.

Phenotypic and transcriptomic analysis reveals the antibiofilm mechanism of essential oil against and its application in pork preservation.

is a common foodborne pathogen that can result in diarrhea and vomiting. It can easily exist in food production environments and form biofilms that are difficult to remove. This study investigated the inhibitory effect and mechanism of essential oil (LC-EO) against biofilm based on phenotype and transcriptomics, as well as the application in pork preservation.

Repeated intravenous transplantation of human umbilical cord mesenchymal stem cells does not promote tumorigenesis in EGFR-mutated lung cancer mice.

Mesenchymal stem cells (MSCs) are extensively studied in clinical trials for their potential therapeutic applications in degenerative and inflammatory diseases and disorders. Despite the lack of clinical evidence indicating that MSCs induce carcinogenesis, the immunosuppressive and proangiogenic functions of MSCs are considered as potential risks involving immune escape and tumor occurrence in programming tumor microenvironment. Previously, many groups had studied the tumorigenic safety of MSCs, but most of these studies were modeled in immuno-deficient mice with different types and sources of transplanted tumors, leaving varied and controversial conclusions.

U-FISH: a fluorescent spot detector for imaging-based spatial-omics analysis and AI-assisted FISH diagnosis.

Imaging-based spatial-omics advances biomedical discoveries with subcellular resolution and high sensitivity, but accurately identifying signal spots from diverse images remains challenging. We develop U-FISH, a deep learning method that enhances images for consistent spot detection across various spatial-omics data. We establish a comprehensive FISH image dataset from seven spatial-omics methods.

Mammalian conservation of endogenous G-quadruplex reveals their associations with complex traits.

Background: DNA G-quadruplexes (G4s) are four-stranded DNA structures. Endogenous G-quadruplexes (eG4s) have been identified as pivotal regulatory elements for gene expression in the human genome. The measurement of evolutionary conservation can be employed to ascertain the functional relevance of putative regulatory elements.

Integrated fecal macrogenomic and metabolomic analyses reveal celiac disease flora and metabolic profiles associated with Chinese populations.

Background And Aims: Genes and gluten intake are necessary but not sufficient to cause celiac disease (CeD), and altered intestinal flora is an additional risk factor for the development of CeD. The present study was conducted to investigate the intestinal flora and metabolic characteristics of CeD among the Chinese population, with the use of CeD patients from Xinjiang, China.

Methods: Macrogenomic sequencing was performed to analyze the composition and differences of the intestinal flora of 40 CeD patients and 40 healthy subjects.

Polymerase-based DNA reactions for molecularly computing cancerous diagnostic valences of multiple miRNAs.

Conventional miRNA-based diagnostic methods often treat all biomarkers equally, overlooking the fact that each miRNA contributes differently to disease classification. This differential diagnostic importance is captured by the concept of Cancerous Diagnostic Valence (CDV)-a metric that quantifies both the direction (oncogenic or protective) and magnitude of each miRNA's association with cancer. Here, we introduce a polymerase-based DNA molecular computing system that directly encodes and integrates CDVs to perform weighted molecular classification of non-small cell lung cancer (NSCLC).

Hyperbaric oxygen augments Doxil antitumor efficacy by reducing tumor-induced lactate.

Compared to healthy individuals, cancer patients exhibit significantly elevated lactate. While numerous studies have revealed the critical role of lactate in fostering an immunosuppressive tumor microenvironment, the effects of lactate on cancer nanomedicine (e.g.

Enhancement of Free Fatty Acids Production in Using the CRISPR/Cas9-Based Base Editor.

is a promising cell factory to produce various value-added chemicals, including fatty acid derivatives. However, their metabolic engineering development has been hindered by the limited availability of genetic tools. In this study, an accurate and specific gene-editing tool, CRISPR/Cas-based cytidine base editor (CBE) system, was developed for the first time in to broaden its genetic toolbox.

pH-Responsive carrier-free nanoparticles based on teicoplanin and borneol for enhanced MRSA infectious wound healing.

Bacterial infections severely threaten human health, and the drug resistance induced by long-term high-dose antibiotic use is a critical issue, which necessitates new antibacterial strategies. In this study, pH-responsive carrier-free nanoparticles (BF-TEI NPs) are fabricated based on the Schiff-base bonding between the hydrophilic antibiotic teicoplanin (TEI) and the hydrophobic antibacterial borneol 4-formylbenzoate (BF). Self-assembled BF-TEI NPs enable synchronous release of BF and TEI in infected sites for synergistic antibacterial effects acidic microenvironment-triggered Schiff-base bond cleavage.

Light-Activated CD36-Targeted Cuproptosis Triggers Metabolic-Immune Synergy for Precision Breast Cancer Therapy.

Breast cancer therapy confronts dual challenges of metabolic plasticity-driven drug resistance and immunosuppression. To address this, we developed DCP-TPP, a therapeutic nanoplatform that integrates dysregulation of copper homeostasis and lipid metabolism for precise breast cancer therapy. Leveraging the overexpression of cluster of differentiation 36 (CD36) in breast cancer cells, DCP-TPP employs fatty acid camouflage (PCM) to deliver disulfiram (DSF) and photothermal CuBiS to cancer cells and features triphenylphosphonium (TPP) modification for targeted mitochondrial drug delivery.

Quantification of Fatty Acids from Arctium lappa L. Leaves by C qNMR and HPLC-CAD.

Arctium lappa L. (burdock) leaves is effective in the traditional treatment of stroke. Fatty acids are abundant in burdock leaves, and may play an important role in the treatment of stroke.

Modular multi-enzyme cascades enable green and sustainable synthesis of non-canonical amino acids from glycerol.

Non-canonical amino acids (ncAAs) bearing diverse functional groups hold transformative potential in drug discovery, protein engineering, and biomaterial science. However, their industrial-scale production remains constrained by the inefficiency, high cost, and environmental burden of conventional chemical and enzymatic methods. Here, we present a modular multi-enzyme cascade platform that leverages glycerol-an abundant and sustainable byproduct of biodiesel production-as a low-cost substrate for ncAAs synthesis.