Enhancement of sulforaphene stability via hydroxypropyl methylcellulose-based solid dispersions: exploiting hydrophobic cavities and hydrogen bonding-mediated water sequestration.

Sulforaphene (SFE) is a bioactive isothiocyanate, known for its cancer-preventive, anti-inflammatory, and antioxidant properties. However, the application of SFE is severely limited by its poor stability. Hydroxypropyl methylcellulose (HPMC), an amphiphilic carbohydrate polymer, has potentials to enhance the stability of SFE and the loading capacity. Herein, HPMC-based solid dispersions (SDs) of SFE were developed and characterized primarily using differential scanning calorimetry, Raman spectrum, and atomic force microscope. The decomposition dynamics exhibited improved stabilities of SFE through the incorporation of HPMC, and the shelf life (t) of SFE was extended up to 596.5 days at 4 °C with 2.5 % water. The stabilization mechanism was explored using Raman mapping and molecular dynamics simulations. The isothiocyanate group of SFE aggregated around the methyl groups of HPMC (approximately 0.4-0.5 nm). Meanwhile, the hydroxy and hydroxypropyl groups of HPMC captured free water through hydrogen bonding (with a determined distance of approximately 0.25 nm), decreasing the reaction possibility between water and SFE. The effects of these groups were proved using cellulose with various substituent. Furthermore, the compressed formulation of the SD exhibited superior controlled-release characteristics. This study provided innovative strategies for developing efficient formulation of SFE, and demonstrated significant potential for applications in foods and pharmaceuticals.