regulates heat-resistance and growth in lettuce ( L.).

Lettuce prefers a cold and cool climate, and high temperatures can lead to many problems such as tip burn that decrease yield and quality. NAC (NAM, ATAF1/2, and CUC2) proteins are important regulatory factors in abiotic stress responses. In our previous transcriptomic analysis, we identified that is involved in the response to heat stress in lettuce.

Polymerase-based DNA reactions for molecularly computing cancerous diagnostic valences of multiple miRNAs.

Conventional miRNA-based diagnostic methods often treat all biomarkers equally, overlooking the fact that each miRNA contributes differently to disease classification. This differential diagnostic importance is captured by the concept of Cancerous Diagnostic Valence (CDV)-a metric that quantifies both the direction (oncogenic or protective) and magnitude of each miRNA's association with cancer. Here, we introduce a polymerase-based DNA molecular computing system that directly encodes and integrates CDVs to perform weighted molecular classification of non-small cell lung cancer (NSCLC).

Pulse wave-driven machine learning for the non-invasive assessment of coronary artery calcification in patients with end-stage renal disease undergoing hemodialysis.

Background: Coronary artery calcification (CAC) represents a major cardiovascular risk in patients with end-stage renal disease (ESRD) undergoing hemodialysis. Given that radial artery pulse waveforms can reflect vascular status, this study aimed to evaluate their utility in the non-invasive assessment of CAC severity.

Methods: 58 patients with ESRD undergoing hemodialysis were enrolled.

Application of chemotherapy combined with immunotherapy in first-line treatment of advanced esophageal cancer: fluorouracil or paclitaxel? - a meta-analysis.

Introduction: Immunotherapy combined with chemotherapy is now the first-line standard for metastatic/recurrent esophageal cancer, with rapid immunotherapy advancements significantly improving patient outcomes. International guidelines recommend platinum-based combinations with either 5-fluorouracil or paclitaxel as first-line treatments. Currently, no head-to-head studies directly compare fluorouracil and paclitaxel regimens in esophageal cancer.

[Value of monocyte-to-lymphocyte ratio and neutrophil-to-lymphocyte ratio in evaluating the severity and prognosis of pediatric viral encephalitis].

Objectives: To investigate the value of peripheral blood monocyte-to-lymphocyte ratio (MLR) and neutrophil-to-lymphocyte ratio (NLR) in evaluating the severity and prognosis of pediatric viral encephalitis (VE).

Methods: A retrospective analysis was performed for the clinical data of 268 children with VE who were admitted to the Department of Pediatrics, Zhucheng People's Hospital, from February 2020 to September 2024. According to the Glasgow Coma Scale (GCS) score, the children were divided into critical group (109 children; GCS score ≤8) and non-critical group (159 children; GCS score >8).

The Contradictory Effects of SPTLC1 on Clear Cell Renal Carcinoma Sensitivity to Sunitinib Mediated by Androgen Receptor.

Serine palmitoyltransferase long chain-1 (SPTLC1) is a key enzyme in ceramide synthesis, previously identified as a suppressor of tumorigenesis in clear cell renal carcinoma (ccRCC). Although elevated levels of very long-chain ceramides are associated with the canonical multidrug resistance in ccRCC, the specific role of SPTLC1 in modulating the sensitivity of ccRCC to sunitinib remains unclear. In this study, we found that SPTLC1 overexpression could enhance the sensitivities of 786-O and OSRC-2 cells to sunitinib via downregulating CerS2 expression and long-chain ceramide levels.

Genetic Association of CANT1 Gene with Scoliosis: An Integrative Study Involving Methylation, Immune Factors, and Metabolites.

The CANT1 gene, encoding a nucleotidase linked to calcium signaling, is crucial for spinal development, with its deficiency causing skeletal developmental issues. However, the relationship between CANT1 and scoliosis remains unclear. This study aims to explore the role of CANT1 in scoliosis from a genetic perspective and its potential as a therapeutic target.

Rapid Purification Strategies for Monoclonal Antibodies: A Case Study.

Monoclonal antibodies (mAbs) are widely used in drug development for their precise targeting and lower adverse effects. Market competition drives demand for more efficient mAb production processes. We developed a mAbs purification platform integrating protein A membrane chromatography with sequential anion/cation exchange.

Molecular Damping Effect of Trace Additives Enhances Zinc Anode Stability Under High Depth of Discharge.

Resolving the severe issues such as electric field distortion, dendritic zinc growth, and uneven zinc deposition under high depths of discharge (DOD) has become a significant hurdle of the aqueous zinc-ion batteries (ZIBs). To address these challenges, an interfacial regulation strategy is proposed based on the molecular damping effect, in which a trace amount of weakly adsorbing additive is employed to stabilize the Zn anode interface by mitigating energy shocks and ionic disturbances induced by electric field fluctuations. Trace perfluorinated PSVE (erfluoro-3,6-dioxa-4-methyloct-7-enesulphonyl fluoride) is introduced to the traditional ZnSO electrolyte to optimize Zn deposition behavior on the zinc anode.

In Situ Formation of Mechanically Interlocked Heterointerfaces with Ultrahigh Bonding Strength for Mg-Based Multilayered Sheets.

Tailoring heterointerfaces is crucial for developing novel laminated metal composites (LMCs) with synergistic multifunctional structural properties. This study pioneers a novel approach to fabricating Mg-Ta incompatible LMCs via crystal orientation regulation, achieving in situ formed serrated mechanically interlocked interfaces with exceptional bonding strength (80.5 MPa) through hot-rolling.

TGF-β1/IL-11 positive loop between renal tubular epithelial cell and fibroblast promotes the ectopic calcification of renal interstitial fibroblasts in rats.

Randall's plaque (RP) is one of the key causes of calcium oxalate (CaOx) kidney stones formation and recurrence characterized by osteogenic differentiation and fibrosis in renal papilla area. Previous studies have found that IL-11 could promote the osteogenesis of fibroblasts and function as a pro-fibrotic cytokine. Moreover, IL-11 expression is increased in RP tissues.

ASFmeter: A Portable A-Mode Ultrasound Device for Abdominal Subcutaneous Fat Thickness Measurement.

Background: Obesity is a global health concern linked to an elevated risk of chronic diseases. Abdominal subcutaneous fat (ASF) thickness serves as a key indicator for obesity assessment; however, existing measurement methods often lack simplicity and accessibility.

Methods: We developed the ASFmeter, a portable, low-cost A-mode ultrasound device designed for rapid ASF thickness measurement.

Emergence of multidrug resistance and genomic analysis of azithromycin-resistant non-typhoidal Salmonella from diverse sources in China, 2006-2018.

Azithromycin is commonly recommended for the treatment of invasive salmonellosis, although the emergence of azithromycin resistance has become a new public health issue, it was seldomly investigated in China. This study analyzed 1230 non-typhoidal Salmonella (NTS) isolates from diverse sources in China (2006-2018), identifying 101 azithromycin-resistant NTS isolated from chicken, pork and human with minimum inhibitory concentration (MIC) values of 32-64 μg/mL of azithromycin across seven serotypes, primarily ST17 S. Indiana (n = 68) and ST52 S.

Improving neuronal recovery in spinal cord injury with NEP1-40-modified neural stem cells through RhoA/ROCK signaling pathway modulation.

To evaluate the therapeutic potential of NEP1-40-expressing neural stem cells (NSCs) in improving outcomes in spinal cord injury (SCI) by modulating the RhoA/ROCK signaling pathway. NEP1-40 was overexpressed by transfecting isolated NSCs with NEP1-40 lentivirus, and their differentiation following Nogo-A treatment was assessed via Tuj-1 immunofluorescence. The effects of NEP1-40 overexpression on axonal damage of differentiated NSCs and DRG neurons were examined using Tuj-1 and F-actin immunofluorescence, along with Western blotting for GAP-43, MAP-2, and APP.

Integrative transcriptomic analysis reveals alternative splicing complexity and transcriptomic diversity in porcine placentas across altitudes.

High-altitude hypoxia provides a natural laboratory for studying adaptation in plateau mammals. As an interface for oxygen and nutrient exchange, placenta plays a critical role in fetal development. While high-altitude adaptation in systemic physiological responses and cardiopulmonary tissues has been well-studied, a comprehensive landscape of porcine placental transcriptomic diversity and alternative splicing (AS) complexity across altitudes remains lacking.

Componential modeling of argumentative essay writing from multiple online sources: a Bayesian network approach.

Introduction: Writing argumentative essays using multiple sources is a critical skill for college students, yet it remains a significant challenge. Despite previous research acknowledging this difficulty, the specific dynamics of the argumentative essay writing process and where breakdowns occur remain unclear.

Methods: College students wrote argumentative essays on a controversial topic after reading multiple documents.

Targeting 5-HT to Alleviate Dose-Limiting Neurotoxicity in Nab-Paclitaxel-Based Chemotherapy.

Chemotherapy-induced peripheral neurotoxicity (CIPN) is a severe dose-limiting adverse event of chemotherapy. Presently, the mechanism underlying the induction of CIPN remains unclear, and no effective treatment is available. In this study, through metabolomics analyses, we found that nab-paclitaxel therapy markedly increased serum serotonin [5-hydroxtryptamine (5-HT)] levels in both cancer patients and mice compared to the respective controls.

Tailoring diblock copolymers for efficient siPLK1 delivery and enhanced gene therapy of orthotopic osteosarcoma.

Osteosarcoma (OS) is a primary malignant bone tumor characterized by its aggressive local destruction and high metastatic potential. RNA interference (RNAi)-based therapeutics show great promise for treating OS; yet the challenge lies in developing safe and efficient delivery systems that can achieve effective siRNA delivery and therapeutic outcomes, particularly in orthotopic OS models. Herein, we introduce a diblock copolymer with precisely designed block composition and length that simultaneously fulfills the multiple requirements for siRNA delivery, both and .

Removal of homodimer species with MabSelect VH3 during the purification of an asymmetric bispecific antibody.

By-products are continuously generated during the manufacturing process of bispecific antibodies, among which homodimers represent the most critical impurity. The effective removal of homodimers is essential, as their presence compromises product purity and may adversely affect therapeutic safety and efficacy. Affinity chromatography, which exploits the highly selective molecular interactions between target antibodies and immobilized ligands, remains the gold-standard purification technique for monoclonal (mAbs) and bispecific antibodies (bsAbs).

Real world study on efficacy and safety of surufatinib in advanced solid tumors evaluation.

Surufatinib is a novel, China-developed small-molecule tyrosine kinase inhibitor that demonstrates high selectivity for VEGFR, FGFR1, and CSF1R. Surufatinib has been approved for the treatment of neuroendcrine tumors, including pancreatic neuroendocrine tumors (PNEN) and non-pancreatic neuroendocrine tumors (N-pNEN). The purpose of this retrospective study is to assess Surufatinib's safety and effectiveness in patients with various advanced solid malignancies.

Systematic Review and Meta-Analysis of the Effect of Motor Intervention on Cognition, Communication, and Social Interaction in Children with Autism Spectrum Disorder.

Aims: Conduct a systematic review and meta-analysis on the effects of motor intervention on social, communication, and cognitive skills in individuals (0-21 years) with autism spectrum disorder (ASD).

Methods: Seven databases were used to search for randomized control trials (RCT) implementing a motor intervention for children with ASD; and measured social, communication, and cognitive outcomes. Twenty-three RCTs were selected with 66 outcomes and 636 participants (range of mean age: 4.

Innovative dura-splitting strategy for resection of spinal meningioma along with the inner layer of dura mater.

Objective: Spinal meningioma is a common extramedullary tumor that can theoretically be cured by complete microsurgical resection. However, complete resection of the tumor is challenging with traditional surgical strategies due to the variable relative position of meningioma and spinal cord, as well as the risk of CSF leakage, which leads to high recurrence rates in the long term. The aim of this study was to explore an innovative dura-splitting strategy to resect the tumor, along with the attached inner dural layer, and evaluate the clinical prognosis.

Mitochondria-targeting natural product rhein conjugated with dichloroacetate as the dual inhibitor of glycolysis and oxidative phosphorylation to off energize cancer cells and induce ROS storm.

Metabolic reprogramming emerges as a remarkable hallmark of cancer cells and exhibits potential in the development of metabolic modulators. Numerous small-molecule inhibitors mainly target reversing the dominant-glycolysis pathway. However, energy metabolic adaptation that facilitates the alternation of metabolic phenotypes from glycolysis to oxidative phosphorylation (OXPHOS) undermines treatment efficacy.

DNA Molecular Computing with Weighted Signal Amplification for Cancer miRNA Biomarker Diagnostics.

The expression levels of microRNAs (miRNAs) are strongly linked to cancer progression, making them promising biomarkers for cancer detection. Enzyme-free signal amplification DNA circuits have facilitated the detection of low-abundance miRNAs. However, these methods may neglect the diagnostic value (or weight) of different miRNAs.