Serotype 14: A Nonnegligible Zoonotic Population.

is an important zoonotic pathogen that can cause severe infections in both humans and pigs. The prevalence of serotype 14 in sporadic cases in China has been gradually increasing during recent years. However, the current understanding of serotype 14 is limited.

Development and validation of a deep-learning algorithm for rule-in and rule-out coronary artery disease based on electrocardiogram without evidence of myocardial ischemia.

Background: Current coronary artery disease (CAD) guidelines recommend to rule-out or rule-in patients for further examination by assessing a pretest probability (PTP) ≤ 5 % or ≥ 15 %. We developed and validated a deep-learning algorithm for rule-in or rule-out based on electrocardiogram (ECG) without myocardial ischemia evidence.

Methods: Between October 2019 and June 2022, data from two centers (Fuwai Hospital [Beijing] and Yunnan Fuwai Hospital) of CAD-suspected patients undergoing either coronary angiography or coronary computed tomography were used.

High-flux and anti-fouling membrane distillation membrane with VOC capture ability enabled by ZIF-8.

Seawater desalination and wastewater purification from brackish water, shale gas extraction, and industrial processes remain vital but challenging. Membrane distillation offers a promising solution yet struggles with low flux, membrane fouling, and the permeation of volatile organic compounds alongside water vapor. Here, we introduce an omniphobic ZIF-8 composite membrane that traps volatile organic compounds under vacuum through crystal phase transitions and enhances water evaporation via linkage swing within hydrophobic micropores.

Honeysuckle-derived carbon dots as fluorescent nanoprobes for determination of sunset yellow and cell imaging.

Sunset yellow (SY) is a widely used food and pharmaceutical colorant, but concerns over its safety highlight the need for sensitive and accurate detection methods. In this work, carbon dots were synthesized from the herbal medicine Honeysuckle (H-CDs). H-CDs exhibited excellent luminescent properties, structural stability, and remarkable selectivity and sensitivity toward SY.

Serum Visfatin Level and Cardiac Valve Calcifcation in Hemodialysis Patients.

Background: Cardiac valve calcification is a serious complication in patients with cardiovascular disease. This study investigated the relationship between visfatin levels and cardiac valve calcification in individuals undergoing hemodialysis.

Methods: We conducted a cross-sectional study at Beijing Luhe Hospital of Capital Medical University, enrolling 339 maintenance hemodialysis patients between June 2023 and May 2024.

Psychological crisis and emergency response in public health emergencies: a case study of the Mpox epidemic.

Background: The frequent occurrence of social infectious diseases not only threatens the lives and health of the public but also brings about a more serious psychological crisis for the public. Understanding the public's psychological reactions during public health emergencies is crucial to developing an effective crisis management strategy.

Methods: This study focuses on the monkeypox epidemic and uses Python to crawl the topic data on monkeypox on Weibo.

Secondary amine-activated ferrate(VI) for isoquinoline degradation: Relationship between molecular structure and reactive performance.

This study elucidates the electronic structure-activity relationship between secondary amines and ferrate(VI) (Fe(VI)) activation. Comparative experiments demonstrated that pyrrolidine (Py) significantly outperformed diethylamine (Di) in enhancing Fe(VI)'s oxidation capability across various dosage conditions, pollutants, and water matrices. Specifically, for isoquinoline (IQL) degradation, Py-Fe(VI) achieved an approximately 7-fold higher rate constant than Fe(VI) alone, versus a 2-fold improvement observed with Di-Fe(VI).

Parthenolide inhibits Hsp90α ATPase activity and overcomes acquired BRAF-inhibitor resistance in cutaneous melanoma.

Background: Oncogenic mutations in the BRAF gene, particularly the V600E mutation, are present in roughly half of metastatic cutaneous melanoma cases. BRAF inhibitors (BRAFi) have shown significant clinical efficacy; however, their long-term effectiveness is frequently limited by the development of resistance. Parthenolide, a sesquiterpene lactone derived from medicinal herbs, has previously been reported to exhibit anti-melanoma effects and to disrupt signaling pathways associated with BRAFi resistance.

Macrophage and keratinocyte cell assays suggest that the supercritical CO extract of black soybean possesses anti-inflammatory and skin barrier-protective effects.

Atopic dermatitis (AD) is a prevalent inflammatory skin disorder with limited treatment options, highlighting the need for alternative therapeutic strategies. Traditionally, black soybean oil, prepared from Glycine max through high-temperature dry distillation, has been used in Asian medicine to relieve AD symptoms. However, this preparation method poses safety concerns due to the potential formation of carcinogenic by-products.

Single-Cell Multi-Omics Identifies Specialized Cytotoxic and Migratory CD8 Effector T Cells in Acute Myocarditis.

Background: Acute myocarditis (AM), particularly fulminant myocarditis (FM), is an infrequent but life-threatening cardiac inflammation, with limited effective precision-targeted treatments available. This urgent clinical challenge has prompted further investigations into the mechanisms underlying this pathology to develop novel therapeutic approaches.

Methods: We enrolled 40 patients diagnosed with AM (24 mild, 16 fulminant) between December 2022 and November 2023.

Development of a Modular Ribonucleoprotein Complex as a General Strategy to Deliver RNAi Therapeutics.

RNAi therapeutics can potentially address undruggable diseases. However, their full potential is stymied by delivery challenges. An siRNA delivery platform by remodeling the human U4 small nuclear ribonucleoprotein complex (snRNP) is developed.

Novel CD19 Fast-CAR-T cells vs. CD19 conventional CAR-T cells for the treatment of relapsed/refractory CD19-positive B-cell acute lymphoblastic leukemia.

Background: Treatment with chimeric antigen receptor-T (CAR-T) cells has shown promising effectiveness in patients with relapsed/refractory B-cell acute lymphoblastic leukemia (R/R B-ALL), although the process of preparing for this therapy usually takes a long time. We have recently created CD19 Fast-CAR-T (F-CAR-T) cells, which can be produced within a single day. The objective of this study was to evaluate and contrast the effectiveness and safety of CD19 F-CAR-T cells with those of CD19 conventional CAR-T cells in the management of R/R B-ALL.

The Role of TLR4 in Lung Epithelial Cell Injury Caused by Influenza Virus Combined with .

Influenza A virus (IAV) is a major cause of respiratory illness in humans and animals. Secondary bacterial infections, especially those caused by (SA), significantly increase influenza-related morbidity and mortality. However, the mechanisms underlying these co-infections remain unclear.

LARS1 lactylation inhibits autophagy by activating mTORC1 to promote podocytes injury in diabetic kidney disease.

Diabetic kidney disease (DKD) progression is predominantly attributed to podocyte injury. However, the underlying mechanisms remain inadequately understood. DKD patients exhibit elevated lactate concentrations due to impaired glucose metabolism.

Efficacy and Safety of Hetrombopag Versus Thrombopoietin in Promoting Platelet Engraftment After Allogeneic Hematopoietic Stem Cell Transplantation: A Prospective, Multicenter, Randomized Controlled Clinical Trial.

Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is pivotal for hematological malignancies but faces challenges in delayed platelet engraftment, poor graft function (PGF), and bleeding risks. Hetrombopag, a thrombopoietin receptor agonist, was evaluated to enhance platelet recovery posttransplant. Patients undergoing allo-HSCT were randomized on Day 3 Post-Infusion into low-dose (2.

Mesenchymal Stromal Cells and Graft-versus-Host Disease: Preclinical and Clinical Studies.

Graft-versus-host disease (GVHD) remains one of the most severe complications following allogeneic hematopoietic stem cell transplantation (HSCT), significantly contributing to transplant-related morbidity and mortality. While systemic corticosteroids are the standard first-line therapy, a substantial proportion of patients develop steroid-refractory GVHD, which is associated with poor clinical outcomes. Mesenchymal stromal cells (MSCs), a heterogeneous population of multipotent stromal cells derived from diverse tissue sources, have garnered increasing attention due to their immunomodulatory capacity and ability to promote tissue repair.

RACGAP1 promotes tumor progression by influencing neutrophil recruitment and tumor cell proliferation in colorectal cancer.

Colorectal cancer (CRC) progression is closely associated with the dynamic evolution of the tumor microenvironment. As a key component of the tumor microenvironment, tumor-associated neutrophils exhibit protumor properties and dynamically regulate malignant processes by secreting bioactive mediators that coordinate interactions among immune cells. RACGAP1, a cytoplasmic division protein, is highly expressed in various cancers.

Transcranial photobiomodulation improves functional brain networks and working memory in healthy older adults: An fNIRS study.

Background: Transcranial photobiomodulation (tPBM), as a novel non-invasive neurostimulation technique, has shown the compelling potential for improving cognitive function in aging population. However, the potential mechanism remains unclear. Neuroimaging studies have found that tPBM-induced physiological changes exist in both targeted and non-targeted brain areas, suggesting the necessity of understanding the modulation mechanism from the perspective of the whole brain level.

Emergence of multidrug resistance and genomic analysis of azithromycin-resistant non-typhoidal Salmonella from diverse sources in China, 2006-2018.

Azithromycin is commonly recommended for the treatment of invasive salmonellosis, although the emergence of azithromycin resistance has become a new public health issue, it was seldomly investigated in China. This study analyzed 1230 non-typhoidal Salmonella (NTS) isolates from diverse sources in China (2006-2018), identifying 101 azithromycin-resistant NTS isolated from chicken, pork and human with minimum inhibitory concentration (MIC) values of 32-64 μg/mL of azithromycin across seven serotypes, primarily ST17 S. Indiana (n = 68) and ST52 S.

Multi-omics analysis unraveling stemness features associated with oncogenic dedifferentiation in 12 cancers.

Tumorigenesis is typically accompanied by cellular dedifferentiation and the acquisition of stem cell-like attributes. However, few studies have comprehensively evaluated the putative relationships between these characteristics and various cancers. Here, we integrated gene expression and DNA methylation quantitative trait loci (cis-eQTL and cis-mQTL) data from the blood to perform multi-omics Mendelian randomization analysis.

Berberine@AgNPs@Carboxylated chitosan hydrogel dressing with immunomodulatory and anti-biofilm properties promotes wound repair in drug-resistant bacterial infections.

Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterial strain resistant to multiple antibiotics frequently encountered in clinical settings. Excessive antibiotic use has increased bacterial resistance, leaving a lack of effective treatments for MRSA infections. MRSA often colonizes the surface of skin wounds, resulting in chronic inflammation and protracted wound healing.

LARS1 Promotes Tubular Epithelial Cells Epithelial Mesenchymal Transition in Chronic Kidney Disease by Inhibiting Lipophagy.

Tubulointerstitial fibrosis (TIF), a critical pathological hallmark in progressive chronic kidney disease (CKD), may be potentiated by renal lipid metabolism dysregulation and ectopic lipid deposition, though these processes likely exhibit bidirectional interactions with fibrotic progression Lipophagy is a type of selective autophagy that specifically recognizes lipid droplets and is accountable for lipid stability and metabolism. It serves as a link between lipid metabolism and autophagy. It was found that a positive correlation between elevated LARS1 expression and the severity of renal interstitial fibrosis in CKD patients.

Tracking Acute Rejection in Heart Transplant Recipients by Using Donor-Derived Cell-Free DNA: A Promising Approach.

Biomarkers play a crucial role in posttransplant monitoring as they enable early detection of graft dysfunction and rejection, thereby facilitating personalized therapeutic interventions. Although recent studies have demonstrated the potential of donor-derived cell-free DNA in monitoring acute rejection episodes in heart transplant recipients, further investigations are required to enhance its diagnostic accuracy and clinical applicability. Comprehensive clinical trials are warranted to establish standardized threshold values and evaluate the diagnostic utility of donor-derived cell-free DNA in identifying various patterns of allograft injury.