Dual-Mode Hybrid Discharge Plasma-Activated Injectable Hydrosol for Enhanced Immunotherapeutic Cancer Therapy.

Although cold atmospheric plasma is a promising therapeutic technique for tumor immunotherapy via reactive oxygen and nitrogen species (RONS), the challenges associated with the generation and delivery of these RONS hamper clinical adoption. Herein, a dual-mode hybrid discharge plasma-activated sodium alginate hydrosols (PAH) is proposed to enhance the antitumor immune response. Gaseous highly reactive RONS are generated by dual-mode hybrid plasma produced by mixed O and NO modes, which are converted into aqueous RONS in PAH via gas-liquid reactions between plasma and hydrosols.

A comprehensive framework of health risk assessment for antibiotic resistance in aquatic environments: Status, progress, and perspectives.

Antibiotic resistance (AR), driven by antibiotics as emerging pollutants, has become a critical global health threat, jeopardizing both environmental and human health. The persistence and spread of AR in aquatic ecosystems are governed by the intricate interplay between antibiotics, antibiotic resistance genes (ARGs), and antibiotic-resistant bacteria (ARB), which collectively influences its occurrence, transportation, and fate in aquatic ecosystems. However, most assessments focus primarily on antibiotics and ARGs, often relying on single-factor criteria while overlooking critical influence factors such as ARG forms, non-antibiotic chemicals, antibiotic pressure, and microbial competition.

Effectiveness of augmented reality technology in improving navigation performance: a systematic review and meta-analysis.

Augmented reality (AR) integrates virtual objects in the real world, allowing users to interact intuitively with navigation information. This study systematically reviewed 13 articles on AR technology published from 2005 to 2024 through meta-analysis, comprising a total of 400 participants, to examine its effectiveness in enhancing navigation performance. Compared with traditional navigation tools, the results showed that AR technology more effectively enhances navigation performance, with the overall effect size calculated as 0.

DNA G-quadruplex profiling in skeletal muscle stem cells reveals functional and mechanistic insights.

Background: DNA G-quadruplexes (G4s) are non-canonical secondary structures formed in guanine-rich DNA sequences and play important roles in modulating biological processes through a variety of gene regulatory mechanisms. Emerging G4 profiling allows global mapping of endogenous G4 formation.

Results: Here in this study, we map the G4 landscapes in adult skeletal muscle stem cells (MuSCs), which are essential for injury-induced muscle regeneration.

Tactile-Transparent Wearable Sensor for Clinician-Friendly Pulse Wave Velocity Monitoring and Cardiovascular Risk Profiling.

Arterial stiffening is an independent risk factor for cardiovascular diseases, particularly affecting organs with low vascular resistance, such as the brain and kidneys. Pulse wave velocity (PWV) is the clinical gold standard for arterial stiffness assessment; however, conventional equipment requires complex setups and trained operators, limiting real-world and point-of-care monitoring. Here, we introduce a tactile-transparent wearable (TTW) sensor that preserves physicians' tactile pulse palpation abilities while providing quantitative cardiovascular risk assessment by integrating flexible Polydimethylsiloxane (PDMS) electrodes and ultrathin graphene oxide dielectric films.

Unleashing pyroelectricity enhancement via phase transition-driven defect alignment in KNN-based ferroelectrics.

The pyroelectric effect plays a critical role in thermal imaging and energy harvesting. Despite extensive efforts to enhance performance through doping and composite engineering, the mechanisms underlying defect dipole coupling with phase structures remain poorly understood, impeding the advancement of defect-engineered symmetry modulation. Here, we report an abnormal pyroelectric phenomenon where the pyroelectric coefficient () increases notably when poling temperature exceeds the orthorhombic-to-tetragonal phase transition temperature () in potassium sodium niobate ceramics.

Tunable Optical Metamaterial Enables Steganography, Rewriting, and Multilevel Information Storage.

In the realm of secure information storage, optical encryption has emerged as a vital technique, particularly with the miniaturization of encryption devices. However, many existing systems lack the necessary reconfigurability and dynamic functionality. This study presents a novel approach through the development of dynamic optical-to-chemical energy conversion metamaterials, which enable enhanced steganography and multilevel information storage.

Associations between prepartum urine pH and periparturient blood calcium concentrations in multiparous Holstein cows.

Introduction: Metabolic alkalosis induced by prepartum diet cations impairs Ca homeostasis in the periparturient cow. Adding anions to prepartum diets reduces blood pH improving periparturient Ca homeostasis. Urine pH generally reflects blood pH and is practical to measure on farm.

Multifunctional Binding Interface Drives Near-Unity CO Selectivity in Acidic CO Electrolysis.

The electrocatalytic carbon dioxide (CO) reduction is challenged by the parasitic hydrogen evolution reaction (HER) especially in acidic media. Here, we elaborate that redox-active isoindigo, acting as a multifunctional co-catalyst, can pre-activate CO-bound intermediates and suppress HER upon the synergistic effects of Lewis acid-base adduct formation, intramolecular hydrogen-bond interaction, and interfacial water structure modulation. Modifying a silver catalyst with isoindigo substantially decreases the energy barrier for CO-to-*COOH conversion, which is regarded as the potential-limiting step of carbon monoxide production.

Enhanced magnetic second-harmonic generation in an ultra-compact plasmonic nanocavity.

Observation of the second-harmonic generation (SHG) from subwavelength metallic structures is often hindered by the interrelations of higher-order multipolar contributions. In particular, the magnetic Lorentz contribution to SHG is often neglected due to the ineffective magnetic field enhancement in electrically resonant structures. Here, we demonstrate a strong Lorentz-driven SHG output at the plasmon-induced magnetic dipolar resonance in inversion-symmetry-broken plasmonic nanocavities.

Seroprevalence of tick-borne diseases in Europe in occupational settings: A systematic review and metanalysis.

Objectives: Tick-borne diseases (TBDs) pose a growing occupational risk due to the expanding geographical range of tick species and their associated pathogens. This study aims to assess TBD seroprevalence among different occupational groups in Europe, identifying high-risk professions and guiding targeted prevention efforts.

Study Design: A systematic review and meta-analysis were conducted following PRISMA guidelines to evaluate TBD seroprevalence in occupational settings across Europe from January 2013 to June 2024.

Beyond the Amyloid Core: Modulation of Aggregate Conformational Diversity by Mutations in the Noncore Regions.

Emerging evidence underscores the regulatory roles of nonamyloidogenic regions in controlling the aggregation dynamics and cytotoxicity of amyloidal proteins, but the mechanism remains unclear. Herein we investigated how flanking sequences modulate the conformational heterogeneity in the p53 238-262 amyloid segment using scanning tunneling microscopy (STM). By comparing the wild-type (wt) and three pathogenic mutations (R248W, R248Q, R249S) in the noncore regions, we reveal that flanking alterations remodel β-sheet aggregates and induce conformational plasticity in β-strand ensembles through the generation of novel conformational substates and selective elimination of existing conformational substates.

Activated Dendritic Cell Membrane-Engineered Nanoformulation of Metallo-Immunomodulators as Lysosome-Targeted Adjuvants for Synergistic Cancer Immunotherapy.

Combination of chemotherapy and cancer immunotherapy has shown substantial clinical promise. However, the immunosuppressive tumor microenvironment (TME) poses a critical barrier to this combination therapy. Here, a tumor lysosome-targeted immunomodulatory strategy based on a biomimetic nanoadjuvant is presented, which effectively overcomes the immunosuppressive TME and demonstrates enhanced therapeutic efficacy when combined with chemotherapy.

SLRanger: an integrated approach for spliced leader detection and operon prediction using long RNA reads.

Spliced leader (SL) trans-splicing occurs in a wide range of eukaryotes and plays a critical role in processing mRNAs derived from operon structures. However, current research on this mechanism remains limited, partly due to the difficulty in accurately identifying genuine SL trans-splicing events. The advent of long-read RNA sequencing technologies, such as direct RNA sequencing by Oxford Nanopore Technologies, offers a more promising avenue for detecting these events with greater resolution.

33 Unresolved Questions in Nanoscience and Nanotechnology.

Significant advances in science and engineering often emerge at the intersections of disciplines. Nanoscience and nanotechnology are inherently interdisciplinary, uniting researchers from chemistry, physics, biology, medicine, materials science, and engineering. This convergence has fostered novel ways of thinking and enabled the development of materials, tools, and technologies that have transformed both basic and applied research, as well as how we address critical societal challenges.

Brightening New Horizons: Luminescent Transition Metal Complexes in Optical Imaging and Theranostic Applications.

Small-molecule fluorescent probes have revolutionized fluorescence imaging in both biological research and clinical applications, allowing the exploration of intricate cellular processes and aiding disease diagnosis and treatment. Advances in the designs of molecular probes have led to the development of fluorescent theranostics that combine therapeutic and diagnostic modalities in a single platform. These dual-functional agents enable simultaneous disease diagnosis, treatment, and monitoring, representing an innovative approach to precision medicine.

Ecological pattern of microalgal communities and associated risks in coastal ecosystems.

Eukaryotic harmful and toxic microalgae, along with their derived toxins, pose significant threats to seafood safety, human health, and marine ecosystems. Here, we developed a novel full-length 18S rRNA database for harmful and toxic microalgae and combined metabarcoding with toxin analyses to investigate the ecological patterns of phytoplankton communities and the underlying mechanism of associated toxic microalgae risks. We identified 79 harmful and toxic species in Hong Kong's coastal waters, with dinoflagellates and diatoms representing the majority of toxic and harmful taxa, respectively.

Prospects of Nanoscience with Nanocrystals: 2025 Edition.

Nanocrystals (NCs) of various compositions have made important contributions to science and technology, with their impact recognized by the 2023 Nobel Prize in Chemistry for the discovery and synthesis of semiconductor quantum dots (QDs). Over four decades of research into NCs has led to numerous advancements in diverse fields, such as optoelectronics, catalysis, energy, medicine, and recently, quantum information and computing. The last 10 years since the predecessor perspective "Prospect of Nanoscience with Nanocrystals" was published in ACS Nano have seen NC research continuously evolve, yielding critical advances in fundamental understanding and practical applications.

TDP-43 binds to RNA G-quadruplex structure and regulates mRNA stability and translation.

TDP-43 is a hallmark protein associated with neurodegenerative diseases. Recent studies revealed TDP-43 as an RNA G-quadruplex (rG4)-binding protein, impacting mRNA transport and function. However, our knowledge of the TDP-43-RNA secondary structure interaction and information on its specific rG4 targets are limited.

The emotional equation: how psychosocial support boosts safety practices in the context of construction 5.0.

Background: Construction 5.0, which emphasizes human-centric technologies and improved collaboration between humans and machines in intelligent construction ecosystems, introduces distinct safety management challenges that necessitate effective emotional resource allocation strategies. This study utilizes job demands-resources theory to investigate how emotional resources are allocated for safety management by examining the relationships among safety practice, psychosocial support, safety participation, and leadership safety behavior.

Enhancer Reprogramming Reveals the Tumorigenic Role of PTPRZ1 in Lung Squamous Cell Carcinoma.

Lung squamous cell carcinoma (LUSC) remains a lethal malignancy with limited therapeutic advancements, underscoring the need to identify novel oncogenic drivers. This study integrates multi-omics analyses-including ChIP-seq, bulk RNA-seq, single-cell ATAC/RNA-seq, and spatial transcriptomics-to delineate enhancer-driven transcriptional networks in 59 matched LUSC tumors and adjacent normal tissues. The analyses identify 3,447 tumor-specific oncogenic enhancers (SOEs) enriched for master transcription factors (SOX2, TP63, KLF5, GRHL2) that orchestrate malignant programs.

Fluorine Modified Zeolitic Imidazolate Framework Enables Long-Life Zn-I Batteries by Suppression of Polyiodide Shuttle.

Aqueous zinc-iodine (Zn-I) batteries have emerged as a promising candidate for large-scale energy storage applications, owing to their inherent safety, cost-effectiveness, and high specific capacity. However, their commercial implementation is severely hindered by the irreversible capacity degradation and limited cycle life, which are caused by the unavoidable iodine shuttle effect resulting from the formation of soluble I species. Herein, we report the synthesis of three-dimensional hexapod-like fluorine-containing zeolitic imidazolate framework (H-F-ZIF) nanoparticles for separator modification to effectively inhibit the iodine shuttle effect.

Immunological dynamics in orthotopic versus subcutaneous murine models of HPV-positive oropharyngeal cancer.

The necessity of reliable preclinical models for evaluating the efficacy of novel therapeutic strategies is imperative. Nevertheless, the degree to which tumor-bearing murine models represent the immunological characteristics of human papillomavirus (HPV)-positive oropharyngeal squamous cell carcinoma (OPSCC) has largely been unexplored. Utilizing single-cell RNA sequencing technology, our research elucidated that subcutaneous (SC) murine models more accurately reflect the early immunogenic phase of human HPV-positive OPSCC, marked by a stage-dependent increase in effector T cell infiltration.

Complex and lasting impacts of heatwaves on life-history traits and fitness in Daphnia magna.

Rapid climatic fluctuations, such as heatwaves, are key drivers of ecological disruption and pose significant physiological challenges to ectothermic organisms, yet their capacity for short- or long-term adaptation and transgenerational effects remain poorly understood. Using the model freshwater zooplankton Daphnia magna, we experimentally tested the physiological resilience, acclimation, and evolutionary responses in D. magna across multiple generations under simulated heatwave conditions.

Innovative Photon-Engineered Fluorescent Tri-Layer Polymeric Coatings for Sub-Ambient Colored Radiative Cooling.

Colored radiative cooling (CRC) materials provide a sustainable solution to thermal management, mitigating global warming while maintaining aesthetic appeal. Nevertheless, conventional CRC materials exhibit reduced cooling efficiency due to their significant sunlight absorption and degraded optical performance in dusty outdoor environments. Developing self-cleaning CRC materials with high cooling performance and vibrant color remains challenging.