Mechanistic insights into aristolochic acid-induced hepatocellular carcinoma: a multi-dimensional analysis integrating network toxicology, machine learning, and molecular dynamics simulation.

Background: Aristolochic acids (AA) are naturally occurring carcinogens found in traditional herbal medicines derived from Aristolochia species. This study explores the potential link between AA and hepatocellular carcinoma (HCC), aiming to uncover key molecular targets driving AA-induced hepatocarcinogenesis.

Methods: Toxicogenomic databases were used to identify AA-related toxicological profiles and targets, which were integrated with HCC-associated gene datasets.

Asiatic acid inhibits endothelial-to-mesenchymal transition in diabetic kidney disease by reducing acetyl-CoA production via targeting ACSS2.

Endothelial-to-mesenchymal transition (EndMT) is a critical contributor of renal fibrosis in diabetic kidney disease (DKD). Asiatic acid (AA), a natural triterpenoid compound, exhibits notable endothelial protective and anti-fibrotic properties; however, its impact on EndMT in DKD remains unclear. This study aimed to investigate the therapeutic effect of AA against EndMT in DKD and the underlying mechanisms.

sp. nov., isolated from the upper respiratory tract of duck.

Five bacterial strains, designated as RCAD1438, RCAD1439, RCAD1670, RCAD1671 and RCAD1672, were isolated from the upper respiratory tract of ducks in Anhui, Shaanxi and Sichuan, China. All strains are Gram-stain-negative, rod-shaped, non-motile, non-spore-forming, aerobic and capsulated. They grow optimally at 37 °C and pH 7.

Asymmetric Total Synthesis of 5,10-seco-Neoansamycin A.

The first asymmetric total synthesis of 5,10-seco-neoansamycin A, a 19-membered cyclic octaketide, was achieved in 20 steps (with several steps being telescoped) and 6.7% overall yield starting from the reported compound, thus leading to the assignment of its absolute configuration. This convergent synthetic approach features late-stage macrolactamization, a judicious application of an asymmetric aldol reaction.

Methyltransferase-Like 3-Mediated m6A Modification of Heat Shock Protein Family A Member 4-Like Facilitates Interleukin-1β-Induced Chondrocyte Injury to Promote Osteoarthritis Progression.

Heat shock protein family A member 4-like (HSPA4L) has been shown to be overexpressed in osteoarthritis (OA) patients, but its role in OA process still unknown. Chondrocytes were stimulated with interleukin-1β (IL-1β) to mimic OA cell model in vitro, and rat was injected with monosodium iodoacetate (MIA) to construct OA rat model in vivo. The expression of HSPA4L, methyltransferase-like 3 (METTL3) and extracellular matrix (ECM)-related markers was examined by qRT-PCR or western blot.

Brucine Inhibits Gastric Cancer via Activation of Ferroptosis Through Regulating the NF-κB Signaling Pathway.

Gastric cancer (GC) is the third leading cause of cancer mortality globally, often presenting with insidious symptoms that lead to late-stage diagnoses, underscoring the critical need for innovative diagnostic and therapeutic strategies. One such avenue is the exploration of ferroptosis, a regulated form of cell death implicated in various pathological conditions and malignancies. In this study, we demonstrate that brucine, an alkaloid derived from Strychnos nux-vomica, exerts significant antitumor effects on GC cells both in vitro and in vivo.

Psychometric Properties of the Chinese Version of the Anhedonia Scale for Adolescents and Identification of the Subpopulation with Prominent Anhedonia.

Objective: Adolescent anhedonia (AA) exhibits distinct characteristics. Currently available anhedonia scales in Chinese are designed solely for adult populations. This investigation assessed the psychometric characteristics of the Chinese Anhedonia Scale for Adolescents (ASA-C) across clinical, subthreshold, and typically developing adolescent cohorts, while establishing its optimal cut-off for prominent anhedonia identification.

The Imaging Evaluation of Left Atrial Appendage: CT Large-Spiral Arterial Late Scan.

Purpose: Compared with retrospective ECG-gated arterial phase scan, to investigate the clinical application value of dual-source CT large-spiral arterial late scan in the imaging evaluation of left atrial appendage (LAA).

Patients And Methods: A total of 108 patients requiring LAA CT angiography (CTA) due to atrial fibrillation (AF) were selected from September 2024 to December 2024, including 52 patients in group A (Flash large-spiral arterial late scan) and 56 patients in group B (retrospective ECG-gated arterial phase scan). All patients underwent double-phase scan.

ERRγ Promotes Multiple Myeloma Survival by Coordinating NF-κB Signaling and Mitochondrial Apoptosis Regulation.

Background: Multiple myeloma (MM) remains a formidable clinical challenge due to its high relapse rate and resistance to existing therapies. Estrogen-related receptor gamma (ERRγ), a nuclear receptor critical for cellular energy metabolism, has been implicated in various cancers. but its role in MM remains unclear.

Hierarchical Co Cu S Nanoflower as Peroxidase Mimics for Advanced Ascorbic Acid Biosensing.

Nanozymes, which possess inherent catalytic properties that are akin to those of natural enzymes, have emerged as promising candidates for biomedical innovation. In this work, we successfully synthesize a Co Cu S nanoflower by the solvothermal and soaking method. Fortunately, through cobalt doping and microstructure design, its morphological structure and active sites have been optimized and adjusted, thus bestowing the Co Cu S nanoflower enhanced peroxidase-mimetic activity.

Coexistence of bullous pemphigoid, intrahepatic cholangiocarcinoma, and alopecia areata: a case report of multifactorial autoimmunity in a surgical context.

Background: Bullous Pemphigoid (BP) is caused by a predominantly Th2-mediated attack on the basement membrane by the production of anti-BP180 and anti-BP230 antibodies. Malignant tumors can exacerbate immune disorders through a variety of potential pathways, including pro-inflammatory responses in the tumor microenvironment, cross-immune responses induced by tumor-associated antigens, and the lifting of immunosuppressive states and activation of underlying autoimmune responses after surgery. Alopecia Areata (AA) is an autoimmune disease caused by T-lymphocyte-mediated destruction of the immune privilege of the hair follicle, specifically involving the immune axes of Th1, Th2 and Th17.

Potential role of 4-hydroxyisoleucine in enhancing fertility in male mice with diet-induced obesity.

Background: Obesity is associated with hormonal imbalance, increased oxidative stress, and inflammation in the testis. These conditions adversely affect sperm quality, leading to impaired male fertility. Therefore, therapeutic interventions to counteract the adverse effects of obesity are crucial.

Global burden of aortic aneurysm attributable to high-sodium diet from 1990 to 2021.

Background: This study aims to investigate the impact of high-sodium diet (HSD) on the global burden of aortic aneurysm (AA), analyze its epidemiological trends across different regions, sexes, and age groups, and project future trends.

Methods: Data were obtained from the Global Burden of Disease Study 2021 (GBD 2021) to assess the disability-adjusted life years (DALYs) and death cases of AA attributable to HSD (AA-HSD) from 1990 to 2021. A decomposition analysis was conducted to quantify the contributions of population growth, aging, and epidemiological changes to the disease burden.

MED10 as a Novel Oncogenic Driver in HCC: Promoting Cell Cycle Progression and Proliferation Through RAF1 Activation.

Background: Mediator complex subunit 10 (MED10) serves as a critical regulator of eukaryotic gene expression by facilitating RNA polymerase II activity. Our investigation aims to characterize MED10's functional contributions and underlying molecular pathways in hepatocellular carcinoma (HCC) development.

Methods: MED10 expression patterns in HCC and their correlation with clinicopathological parameters and patient outcomes were examined using bioinformatics databases and immunohistochemistry.

O-GlcNAcylated YTHDF2 promotes bladder cancer progression by regulating the tumor suppressor gene via mA modification.

Objectives: Bladder cancer is a common malignancy with high incidence and poor prognosis. N-methyladenosine (mA) modification is widely involved in diverse physiological processes, among which the mA recognition protein YTH N-methyladenosine RNA binding protein F2 (YTHDF2) plays a crucial role in bladder cancer progression. This study aims to elucidate the molecular mechanism by which O-linked -acetylglucosamine (O-GlcNAc) modification of YTHDF2 regulates its downstream target, period circadian regulator 1 (), thereby promoting bladder cancer cell proliferation.

FTOregulated mA modification of primiR139 represses papillary thyroid carcinoma metastasis.

Objectives: Increasing detection of low-risk papillary thyroid carcinoma (PTC) is associated with overdiagnosis and overtreatment. N6-methyladenosine (mA)-mediated microRNA (miRNA) dysregulation plays a critical role in tumor metastasis and progression. However, the functional role of mA-miRNAs in PTC remains unclear.

m6A-Mediated Methylation Patterns and Their Association With Obstructive Sleep Apnea in Lung Adenocarcinoma.

Background: Epigenetic regulation significantly affects immune responses in lung adenocarcinoma (LUAD). However, the role of RNA N6-methyladenosine (m6A) modification, especially in obstructive sleep apnea-hypopnea syndrome (OSAHS) within LUAD, is not well understood.

Methods: This study examined m6A modification patterns in 973 LUAD patients using 23 regulatory genes.

Mechanism of Tetrandrine in Ameliorating Hypoxic Pulmonary Hypertension Vascular Remodeling through Transcriptomics and Metabolomics.

Background: Tetrandrine (TET) demonstrates therapeutic potential for hypoxic pulmonary hypertension (HPH); however, its precise pharmacological mechanisms remain unclear. In this study, we aimed to investigate the effects of TET on pulmonary vascular remodeling (PVR) in HPH and elucidate the molecular pathways through which TET ameliorates HPH.

Methods: We established a rat model of HPH and evaluated the therapeutic effects of TET by measuring hemodynamic parameters, assessing right ventricular hypertrophy, and analyzing pathological changes in lung tissue.

The Association between the rs6656401 Locus of the CR1 Gene and Structural Alterations of Brain Effects in Han Chinese Patients with Alzheimer's Disease.

Introduction: The complement receptor 1 (CR1) gene is identified as the one closely associated with Alzheimer's disease (AD). However, there has been no exploration of the imaging alterations associated with the CR1 gene in AD patients of the Han population. The purpose of this study is to investigate the association between the rs6656401 mutation and neuroimaging variations in Han AD patients.

Fenoxaprop-P-ethyl resistance in Alopecurus aequalis: Involvement of GSTF13 in addition to Ile-1781-Leu mutation.

Shortawn foxtail (Alopecurus aequalis Sobol.) is a challenging weed species to manage in wheat production systems globally. In prior research, we identified a field population of A.

Jinggangmycin-induced NlPR-L and NlABD4-L genes regulate fecundity in the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae).

The brown planthopper (BPH), Nilaparvata lugens (Stål), is a typical insecticide-induced resurgence rice pest that causes severe damage to rice in Asian countries. Previous studies have shown that the fungicide Jinggangmycin (JGM), used to control rice sheath blight disease, can stimulate BPH fecundity; however, the molecular mechanism remains to be further explored. In this study, based on transcriptomic analysis, we found that the PI3K-Akt signaling pathway was significantly enriched in BPH after feeding on JGM-treated rice, where the NlPR-L and NlABD4-L genes were significantly upregulated.

CYP72A15 confers resistance against penoxsulam to Echinochloa phyllopogon.

As the weed Echinochloa phyllopogon has rapidly developed multi- and cross-resistance to several herbicides, we aimed to determine the mechanism underlying penoxsulam resistance in weeds. There was no target mutation in the tested population, and P450 enzyme activity was significantly higher in the penoxsulam-treated resistant population, confirming that non-target-site resistance was dominant. The antioxidant enzyme activity of the resistant population was higher than that of the sensitive population following the application of the penoxsulam and cleared HO faster.

Mn-doped carbon dots-based fluorescent-colorimetric dual-mode probes for selective and sensitive detection of Cr(VI) ions and l-ascorbic acid via smartphone-integrated analytical platform.

Background: Hexavalent chromium ions (Cr(VI)), a notorious toxic heavy metal pollutant with proven carcinogenicity, endangers human health and the environment. Meanwhile, l-ascorbic acid (L-AA), a vital biological antioxidant, has abnormal levels closely tied to various diseases. Developing efficient synchronous detection methods for these two key analytes is of great value in clinical and environmental monitoring.

The Optimal Adjuvant Reinfusion Strategy for Aplastic Anemia: Umbilical Cord Blood or Mesenchymal Stem Cells? A Single-Center Retrospective Study.

Aplastic anemia (AA) is a bone marrow failure disorder treated with allogeneic hematopoietic stem cell transplantation (allo-HSCT). Despite improvements in conditioning regimens and GVHD prophylaxis, graft failure and GVHD remain critical challenges. This study compared the efficacy of mesenchymal stem cells (MSCs) and umbilical cord blood cells (UCBs) as adjunctive therapies in 184 AA patients undergoing allo-HSCT.

Integrated network pharmacology, metabolomics and experimental validation to reveal the pharmacological substances and mechanism of Wendan decoction on chronic obstructive pulmonary disease.

Wendan Decoction (WDD), a classic formula for the expectoration of phlegm, has been acknowledged for the management of chronic obstructive pulmonary disease (COPD). Nevertheless, the therapeutic components and mechanism of WDD in COPD remain elusive. Hence, an analytical strategy for extensive investigation of the constituents in WDD combining UFLC-ESI-Q/TOF-MS and subsequent network pharmacology was conducted, and the appropriate preparation of bio-samples was involved with mechanistic bioanalysis including multi-technique metabolomics and molecular biological means, that pivotal factors such as PTGS2, arachidonic acid (AA) metabolism, and PI3K-AKT signaling pathway were recommended as potential central elements for the anti-COPD effects.