Application of laboratory-result-oriented modular teaching model in the training of clinical hematology laboratory interns.

Background: Current teaching models for clinical hematology laboratory interns often prioritize theoretical knowledge dissemination over the practical application of skills directly tied to analyzing and interpreting laboratory results. This approach potentially limits the development of critical clinical reasoning and problem-solving abilities within the framework of quality management systems like ISO 15189. To address these challenges, this study implemented and evaluated a novel laboratory-result-oriented modular teaching model, explicitly integrating ISO 15189 principles.

Metal-free Oxidative Spirocyclization of Pyrrole-2-carboxamides.

A catalyst-free oxidative spirocyclization reaction has been successfully developed for the synthesis of 3,3'-pyrrolidinyl spirooxindole derivatives from readily accessible pyrrole-2-carboxamides. The resulting 3,3'-pyrrolidinyl spirooxindoles can be selectively transformed into hydrogenated spiroindoles via a reduction process. The dual functionality of Selectfluor is noteworthy, as it may serve both as an oxidizing and fluorinating agent and as a precursor for an alkaline promoter.

MRI-Derived Lymph Nodes Morphological and Topological Structure (LNs-MTS) Model for Evaluating Immune Status and Prognosis in Rectal Cancer.

Tumor-draining lymph nodes (LNs) immune status critically influences cancer progression and treatment response, yet reliable non-invasive assessment remains clinically unavailable. To address this critical gap, an MRI-derived LNs morphological and topological structure (LNs-MTS) model is developed to evaluate the immune status of LNs in rectal cancer (RC). Integrating multicenter imaging, transcriptomic, and immunohistochemical data from 1,156 stage I-II RC patients, enhanced immune activation in non-metastatic LNs sized ≥0.

P300 Promotes RUNX1 Transcription to Regulate Autophagy and Drive Corneal Neovascularization.

Purpose: This study aimed to elucidate the regulatory role of Runt-related transcription factor 1 (RUNX1) in corneal neovascularization (CoNV) and its possible mechanisms.

Methods: In VEGF-induced human umbilical vein endothelial cells (HUVECs), lentiviral vectors were used to knock down or overexpress RUNX1, and plasmid transfection was employed to knock down P300. EdU assay, Transwell assay, and tube formation assay were conducted to assess cell proliferation, migration, and tube formation ability, respectively.

Multi-omics approaches provide insights into adaptation to low salinity of intertidal clams.

Climate change is reshaping the population distribution of intertidal organisms, with extreme weather inducing salinity fluctuations that challenge intertidal bivalves. In this study, we employed comparative genomics and transcriptomic analyses to investigate the molecular mechanisms underlying low-salinity adaptation in the razor clam (Sinonovacula constricta). We compiled and analyzed genomic data from 19 molluscan species, classified as either euryhaline or stenohaline based on their salinity tolerance.

Placido Sub-Pixel Edge Detection Algorithm Based on Enhanced Mexican Hat Wavelet Transform and Improved Zernike Moments.

In order to meet the high-precision location requirements of the corneal Placido ring edge in corneal topographic reconstruction, this paper proposes a sub-pixel edge detection algorithm based on multi-scale and multi-position enhanced Mexican Hat Wavelet Transform and improved Zernike moment. Firstly, the image undergoes preliminary processing using a multi-scale and multi-position enhanced Mexican Hat Wavelet Transform function. Subsequently, the preliminary edge information extracted is relocated based on the Zernike moments of a 9 × 9 template.

Lymph nodes molecular subtypes unravel lymph nodes heterogeneity and clinical implications in colorectal cancer.

Lymph nodes (LNs) play a pivotal role in colorectal cancer (CRC) progression and immunity, yet their molecular and functional diversity remains poorly understood. By analyzing 630 LNs and 88 primary tumors from 200 CRC patients across four independent cohorts using bulk and single-cell RNA sequencing, we identify four non-metastatic negative LNs (NLN) subtypes (NLN_C1-C4) exhibiting obviously different immune function and stromal expansion. NLN_C3/C4 are characterized by diminished T and B cell activity and fibroblast-driven fibrosis, with follicular dendritic cell loss contributing to B cell dysfunction.

Workplace mindfulness and clinical belongingness among nursing interns: The mediating role of subjective well-being.

Background: The sense of clinical belongingness significantly impacts the mental health of undergraduate nursing interns. Workplace mindfulness and subjective well-being are considered key factors influencing clinical belonging. Currently, there is no research elucidating how workplace mindfulness and subjective well-being affect clinical belonging.

Transcriptomic WGCNA analyses reveal sectional regulatory mechanisms in giant freshwater prawn Macrobrachium rosenbergii larvae under hypoxia-reoxygenation.

Hypoxia is a considerable challenge for the prawn culture, especially in high-density intensive aquaculture. The giant freshwater prawn, Macrobrachium rosenbergii, is an economically important species in Asia and South America. However, M.

Transcriptomic Analysis Reveals the Key Regulatory Pathways of the Gills and Liver of Grass Carp Under High NaHCOStress.

To elucidate the molecular mechanisms by which grass carp respond to high-concentration NaHCO stress, this study employed RNA-sequencing technology to perform transcriptome analysis on the gill and liver tissues of the treatment group with 30 mmol/L NaHCO and the control group (0 mmol/L). Through sequencing of 12 libraries constructed from 6 gill samples and 6 liver samples, a total of 41.86 GB of high-quality data from gill tissues and 41.

Dual-function electrodes for photo-rechargeable batteries: mechanisms, designs, and applications.

Photo-rechargeable batteries emerge as an integrated solution for simultaneous solar energy harvesting and storage. Central to these systems are dual-function electrode materials that intrinsically combine photoactivity and ion storage capabilities. Through synergistic interactions with electrolytes, these materials enable efficient energy conversion and storage, demonstrating significant potential for next-generation energy technologies.

The intricate interactions between the lungs and gut in patients: unraveling the crosstalk mechanism.

There is a growing body of evidence indicating that the stimulation of one organ can significantly influence the functioning of another. For instance, intestinal complications are frequently observed during respiratory diseases, and conversely, pulmonary complications can arise during intestinal diseases-a phenomenon referred to as lung-gut crosstalk. Patients suffering from mechanical ventilator-induced lung injury, chronic obstructive pulmonary disease (COPD), acute respiratory distress syndrome (ARDS), and other pulmonary conditions have been shown to experience gastrointestinal dysfunction and related disorders.

Identification and Characterization of NAC Transcription Factors Involved in Pine Wilt Nematode Resistance in .

Lamb. is an economically important conifer native to China. However, it is highly susceptible to the pine wood nematode (, PWN), the causal agent of pine wilt disease (PWD), resulting in substantial ecological and economic losses.

LncRNA-miRNA‒mRNA Network in Schizophrenia.

Schizophrenia (SCZ) is a severe mental disorder that significantly impacts the social functioning of patients and can reduce their life expectancy and quality of life. However, the specific causes of SCZ remain unknown, and the evidence indicates that long noncoding RNAs (lncRNAs) play critical roles in its pathogenesis. Analyzing lncRNA expression in peripheral blood samples from patients could reveal the biological mechanisms underlying the disease and help in the identification of biomarkers for early diagnosis and treatment.

Regulating Zn flux and transport behavior with CF-functionalized UiO-66 modified separator for high-performance aqueous zinc-ion batteries.

The increasing demand for more sustainable and safer energy storage systems has driven significant interest in finding alternatives to conventional lithium-ion batteries. Rechargeable aqueous zinc-ion batteries (AZIBs) have emerged as promising candidates due to their high theoretical capacity and the natural abundance of Zn. However, the performance of AZIBs is often limited by the instability of Zn anodes, leading to challenges such as dendrite formation, side reactions, and poor cycling stability.

Global trends and hotspots of adolescent eating disorders: a bibliometric study and visual analysis.

Background: Adolescent eating disorders represent a category of mental illness, marked by elevated mortality and morbidity rates and a significant adverse impact on the quality of life of both patients and their families. As one of the most important directions in eating disorders, adolescent eating disorders have significant research value, social value, and practical significance. Surprisingly, the overall research landscape in this domain has yet to be systematically explored through bibliometric analysis.

[Expression pattern of polyhedrin of BmNPV and involvement of importin α in the nuclear import of Polh].

nucleopolyhedrovirus (BmNPV) is extremely harmful to the silk industry. The , which encodes the polyhedrin (Polh), can be expressed at ultra-high levels and form occlusion bodies in the nucleus, embedding the progeny virus within it. However, the detailed mechanism by which polyhedrin is transported into the host cell nucleus remains unknown.

Advanced Heterogeneous Network-Based Graph Neural Network Framework for Predicting Anti-CRISPR Protein Sequences.

Anti-CRISPR proteins play a crucial role in bacterial-phage interactions by inhibiting the CRISPR/Cas system and thus enhancing phage survival. Accurately predicting these proteins is essential for understanding phage-host immune interactions and progressing CRISPR/ Cas-based technologies. Current approaches primarily analyze proteins individually, which may overlook the intrinsic similarities and potential connections among protein sequences.

Exosome-based liquid biopsy: A new frontier in early cancer diagnosis.

Early-stage diagnosis offers the greatest survival advantage in oncology, and yet conventional liquid-biopsy markers - circulating tumor DNA (ctDNA) and circulating tumor cells (CTCs) - depend on cell death or mechanical shedding and therefore appear late in disease progression. Exosomes, 40-160 nm lipid-bilayer vesicles secreted by viable cells, emerge earlier, outnumber CTCs by several orders of magnitude, and preserve multi-omic cargo that mirrors intratumor heterogeneity. Rapid advances in enabling technologies are driving continual breakthroughs in exosome-based liquid biopsy, laying a solid foundation for its accelerated translation into clinical practice.

Entanglement-enhanced phase sensitivity at low frequencies in a truncated SU(1,1) interferometer.

Quantum metrology harnesses nonclassical resources including squeezed states and quantum entanglement to overcome fundamental limitations imposed by the shot-noise limit (SNL). While conventional optical interferometers for phase detection typically operate in the MHz-frequency regime due to technical noise constraints at lower frequencies, numerous practical applications demand operation in the kHz regime or below. Here, we present a truncated SU(1,1) interferometric configuration that achieves enhanced phase sensitivity in the low-frequency (kHz) domain by exploiting quantum entanglement generated through two-mode squeezed states.

Over 20% Efficient Water-Based Layer-by-Layer Organic Solar Cells with High Thickness Tolerance Enabled by Surfactant Promoted Electrostatic Interaction.

Aqueous processing represents a promising eco-friendly fabrication route for organic solar cells (OSCs), aligning with growing industrial sustainability requirements. While water-dispersed semiconducting nanoparticles (NPs) offer an attractive solution, the essential surfactants required for NP stabilization typically compromise device performance. In this study, surfactant-engineered donor NPs are systematically evaluated for constructing optimized active layers through a sequential layer-by-layer (LBL) deposition approach.

Machine learning-based design, screening, and activity validation of topoisomerase I inhibitors.

Topoisomerase I (TOP I) plays a vital role in maintaining genomic stability and regulating cellular proliferation. Its overexpression in aggressive cancers such as lung, pancreatic, and breast malignancies highlights its value as a therapeutic target. However, the current TOP I inhibitors face limitations including poor hydrolytic stability, significant toxicity, and the emergence of drug resistance.

Effect of dissolved oxygen concentration changes on the ultrastructure of blood cells in Manila clam (Ruditapes philippinarum).

Manila clam (Ruditapes philippinarum) inhabits environments in which dissolved oxygen (DO) concentrations are prone to changes. Hemocytes are important participants in shellfish immune responses and are sensitive to environmental changes. To investigate the effect of DO concentration changes on the ultrastructure of R.

High-Entropy Solid-State Electrolytes for Rechargeable Batteries: Mechanism, Structural Designs, Characterizations, and Applications.

Rechargeable batteries achieve remarkable advancements over the past few decades. Conventional electrolytes suffer from a range of challenges, such as low conductivity and poor thermal stability. Recent studies show that various properties of high-entropy solid-state electrolytes effectively solve these challenges.