MRI-Derived Lymph Nodes Morphological and Topological Structure (LNs-MTS) Model for Evaluating Immune Status and Prognosis in Rectal Cancer.

Tumor-draining lymph nodes (LNs) immune status critically influences cancer progression and treatment response, yet reliable non-invasive assessment remains clinically unavailable. To address this critical gap, an MRI-derived LNs morphological and topological structure (LNs-MTS) model is developed to evaluate the immune status of LNs in rectal cancer (RC). Integrating multicenter imaging, transcriptomic, and immunohistochemical data from 1,156 stage I-II RC patients, enhanced immune activation in non-metastatic LNs sized ≥0.

Lymph nodes molecular subtypes unravel lymph nodes heterogeneity and clinical implications in colorectal cancer.

Lymph nodes (LNs) play a pivotal role in colorectal cancer (CRC) progression and immunity, yet their molecular and functional diversity remains poorly understood. By analyzing 630 LNs and 88 primary tumors from 200 CRC patients across four independent cohorts using bulk and single-cell RNA sequencing, we identify four non-metastatic negative LNs (NLN) subtypes (NLN_C1-C4) exhibiting obviously different immune function and stromal expansion. NLN_C3/C4 are characterized by diminished T and B cell activity and fibroblast-driven fibrosis, with follicular dendritic cell loss contributing to B cell dysfunction.

MACC1 drives metastasis in colorectal cancer by coordinating YKT6-dependent exosome biogenesis and c-Met cargo selection.

Colorectal cancer metastasis remains a major cause of cancer-related mortality, with the Metastasis-Associated in Colon Cancer 1 (MACC1) protein emerging as a critical regulator of tumor progression. Although exosomes are recognized mediators of oncogenic communication, the interplay between MACC1 and exosome biology is yet to be fully explored. This study unveils a dual mechanism through which MACC1 coordinates exosome biogenesis and oncogenic cargo delivery to drive metastatic progression.

Directionally and Substantially Enhanced Photoelectrochemical Responses by Electrostatic Field of (De)protonated Targets.

The ultrasensitive detection of toxic substances in aqueous environments is a critical challenge for environmental monitoring, public health, and sustainable water management. This research reports a hybridized mediation strategy using Au nanoparticles under WO nanosheets to achieve a substantially synergistic enhancement in photoelectrochemical response for detecting herbicides through the combined effects of electrostatic fields and plasmonic near-fields. Beyond optical irradiation, applied potential and near-field effect from Au, another stimulus─a strong electrostatic field induced by the protonation/deprotonation states of herbicide molecules adsorbed on the electrode surface─further accelerates photogenerated carrier separation and transport, thereby suppressing rapid recombination of electron-hole pairs.

Impact of lymph node metastasis on prognosis in colorectal cancer patients with liver metastasis and staging systems Refinement: An international multicenter retrospective cohort study.

Background: The current AJCC staging for colorectal cancer liver metastasis (CRLM) classifies stages IVA, IVB, and IVC based on organ metastasis, disregarding lymph node metastasis (LNM). We evaluated the prognostic impact of LNM in CRLM and proposed incorporating LNM into staging criteria.

Methods: Data were extracted from the SEER database (2010-2017) and a Chinese cohort (2009-2018), including 11,266 CRLM patients (9648 SEER; 1618 Chinese cohort).

The prognostic significance of tumor-specific growth factor in colorectal cancer: an observation study.

Background: The correlation between tumor-specific growth factor (TSGF) and the prognosis of colorectal cancer (CRC)has not been extensively studied. Research was designed to evaluate the potential of TSGF as a predictive marker for the prognosis of patients with CRC.

Methods: The serum TSGF was quantified and stratified into elevated/normal groups based on the established threshold detected using the rate method.

Case report of clostridium difficile infection after rectal resection with ileostomy.

Colorectal cancer is the third most common cancer worldwide, with high incidence and mortality rates. Surgical resection is the primary treatment for rectal cancer. To reduce the occurrence and severity of postoperative complications such as anastomotic leakage, prophylactic ileostomy is often performed concurrently.

Application of copy number variation sequencing combined with whole exome sequencing in prenatal left-right asymmetry disorders.

Background: Left-right (LR) asymmetry disorders present a complex etiology, with genetic factors emerging as a primary contributor. This study aims to explore the genetic underpinnings of chromosomal variants and individual genes in fetuses afflicted with prenatal LR asymmetry disorder.

Methods: Through a retrospective analysis conducted between 2020 and 2023 at Tongji Hospital, Huazhong University of Science and Technology, genetic outcomes of LR asymmetric disorder were scrutinized utilizing copy number variation sequencing (CNV-seq) and whole exome sequencing (WES) methodologies.

Impact of lymph node metastasis on immune microenvironment and prognosis in colorectal cancer liver metastasis: insights from multiomics profiling.

Background: This study aimed to investigate the prognostic impact of lymph node metastasis (LNM) on patients with colorectal cancer liver metastasis (CRLM) and elucidate the underlying immune mechanisms using multiomics profiling.

Methods: We enrolled patients with CRLM from the US Surveillance, Epidemiology, and End Results (SEER) cohort and a multicenter Chinese cohort, integrating bulk RNA sequencing, single-cell RNA sequencing and proteomics data. The cancer-specific survival (CSS) and immune profiles of the tumor-draining lymph nodes (TDLNs), primary tumors and liver metastasis were compared between patients with and without LNM.

Signal regulatory protein α dynamically mediates macrophage polarization facilitated alleviation of ischemic diseases.

Background: macrophage-targeting therapy of ischemic disease has made progress in clinic trial. However, the role and underlying mechanism of pro-inflammatory or anti-inflammatory polarized macrophages in modulating ischemic diseases remain incompletely understood.

Results: here we examine the effect of pro-inflammatory (LPS) and anti-inflammatory (IL-4) macrophage on ischemic diseases in a mouse ischemic hindlimb and heart model, and identify that signal regulatory protein α (Sirpα) modulates macrophage polarization induced angiogenesis via promoting phagocytosis or activating HIF1α nucleus relocation in macrophages, respectively.

Automatic Obstacle Avoidance Trolley System Based on Two-Dimensional ReS/WSe Heterojunction Photodetector.

With the continuous advancement of intelligent vehicle technology, automatic obstacle avoidance (AOA) has become a crucial component of in-vehicle systems. This study introduces an AOA trolley system based on ReS/WSe heterojunction photodetectors. The device with type-II band alignment exhibits an impressive on/off current ratio of 7.

SLC14A1 and TGF-β signaling: a feedback loop driving EMT and colorectal cancer metachronous liver metastasis.

Background: Colorectal cancer (CRC) metachronous liver metastasis is a significant clinical challenge, largely attributable to the late detection and the intricate molecular mechanisms that remain poorly understood. This study aims to elucidate the role of Solute Carrier Family 14 Member 1 (SLC14A1) in the pathogenesis and progression of CRC metachronous liver metastasis.

Methods: We conducted a comprehensive analysis of CRC patient data from The Cancer Genome Atlas and GSE40967 databases, focusing on the differential expression of genes associated with non-metachronous liver metastasis and metachronous liver metastasis.

Xanthohumol and echinocystic acid induces PSTVd tolerance in tomato.

Tomato is a popular vegetable worldwide; its production is highly threatened by infection with the potato spindle tuber viroid (PSTVd). We obtained the full-length genome sequence of previously conserved PSTVd and inoculated it on four genotypes of semi-cultivated tomatoes selected from a local tomato germplasm resource. SC-5, which is a PSTVd-resistant genotype, and SC-96, which is a PSTVd-sensitive genotype, were identified by detecting the fruit yield, plant growth, biomass accumulation, physiological indices, and PSTVd genome titer after PSTVd inoculation.

Impact on CD4+ CD25 High -CD127 low regulatory T (Treg) cells of neoadjuvant therapy for rectal cancer patients.

Background: The efficacy of neoadjuvant therapy for rectal cancer has not been assessed.

Objective: To evaluate proportional changes in peripheral blood immune cells in rectal cancer patients after neoadjuvant therapy and assess the relationship between tumor regression and regulatory T (Treg) cells.

Materials And Methods: Rectal cancer patients who had received neoadjuvant therapy prior to surgery at Shanxi Cancer Hospital between January and September 2018 were enrolled in the study.

5-azacytidine inhibits Sox2 promoter methylation during the induction of Thy-1Lin cells into hepatocytes.

Objective: To investigate the changes and functions of Sox2 gene expression and promoter methylation during induced differentiation of bone marrow mesenchymal stem cells (BMSCs) into hepatocytes (HCs).

Methods: Rat bone marrow Thy-1Lin cells were prepared and divided into control group (directed induction of differentiation into HCs) and experimental group (5-azacytidine intervention induced differentiation). The mRNA expression levels of ALB and Sox2 were detected by fluorescence quantitative polymerase chain reaction (PCR), and the Sox2 gene promoter methylation level was determined by Bisulfite sequencing PCR (BSP).

Associations of exposure to disinfection by-products with blood coagulation parameters among women: Results from the Tongji reproductive and environmental (TREE) study.

Background: Experimental studies have shown that disinfection byproducts (DBPs) induce coagulotoxicity, but human evidence is scarce.

Objective: This study aimed to explore the relationships of DBP exposures with blood coagulation parameters.

Methods: Among 858 women from the Tongji Reproductive and Environmental (TREE) study, urinary dichloroacetic acid (DCAA) and trichloroacetic acid (TCAA) were detected as internal biomarkers of DBP exposures.

Prediction of phase transition and time-varying dynamics of the (2+1)-dimensional Boussinesq equation by parameter-integrated physics-informed neural networks with phase domain decomposition.

A meaningful topic that needs to be explored in the field of nonlinear waves is whether a neural network can reveal the phase transition of different types of waves and novel dynamical properties. In this paper, a physics-informed neural network (PINN) with parameters is used to explore the phase transition and time-varying dynamics of nonlinear waves of the (2+1)-dimensional Boussinesq equation describing the propagation of gravity waves on the surface of water. We embed the physical parameters into the neural network for this purpose.

Distinct brain state dynamics of native and second language processing during narrative listening in late bilinguals.

The process of complex cognition, which includes language processing, is dynamic in nature and involves various network modes or cognitive modes. This dynamic process can be manifested by a set of brain states and transitions between them. Previous neuroimaging studies have shed light on how bilingual brains support native language (L1) and second language (L2) through a shared network.

Strongly Localized Moiré Exciton in Twisted Homobilayers.

Artificially molding exciton flux is the cornerstone for developing promising excitonic devices. In the emerging hetero/homobilayers, the spatial separated charges prolong exciton lifetimes and create out-plane dipoles, facilitating electrically control exciton flux on a large scale, and the nanoscale periodic moiré potentials arising from twist-angle or/and lattice mismatch can substantially alter exciton dynamics, which are mainly proved in the heterostructures. However, the spatially indirect excitons dynamics in homobilayers without lattice mismatch remain elusive.

A novel HBI-based ratiometric fluorescent probe for rapid detection of trifluoroborate.

A 2-(2'-hydroxyphenyl)benzimidazole (HBI)-based ratiometric fluorescent probe, known as BTEP, was synthesized using 5-bromosalicylaldehyde as the raw material Sonogashira coupling and condensation reaction. This probe was designed for rapid detection of boron trifluoride solutions and gases. The N and O coordination atoms in the probe undergo a boron difluoride addition with BF, which affects the process of excited state intramolecular proton transfer (ESIPT) leading to a blue shift of fluorescence emission.

Anomalous transmission and Anderson localization for alternating propagated and evanescent waves at the deep-subwavelength scale.

Deep-subwavelength features have a minimal impact on wave transport in all dielectric systems; thus the homogenization approach was commonly adopted. Recently, the breakdown of effective medium theory (EMT) for the incident wave near the total reflection (TR) angle was demonstrated in a deep-subwavelength dielectric multilayer. Additionally, anomalous transmission was reported at angles exceeding the TR angle when introducing disorder and was attributed to Anderson localization.