Crosstalk between ROS and Ferroptosis, Cuproptosis, and PANoptosis in Liver Cancer: Mechanisms and Therapeutic Strategies.

Hepatocellular carcinoma (HCC) is a common and complex malignant tumor whose etiology is influenced by a variety of factors and exhibits different outcomes during disease progression. HCC is distinguished by the triggering of various cell death pathways, for example ferroptosis, cuproptosis, and PANoptosis. This review provides insights into the current understanding of the interactions between reactive oxygen species (ROS) and these novel mechanisms of cell death in HCC, highlighting their significance in disease pathogenesis and their potentiality as therapeutic goals.

Cervicovaginal Microbiome and HPV: A Standardized Approach to 16S/ITS NGS and Microbial Community Profiling for Viral Association.

16S rRNA next-generation sequencing (NGS) has significantly advanced cervicovaginal microbiome profiling, offering insights into the relationship between vaginal dysbiosis and HPV-associated carcinogenesis. However, reliance on a limited set of 16S hypervariable regions introduces inherent biases that impact results. This study developed standardized workflows for 16S/ITS NGS, with a focus on identifying methodological biases that influence microbial abundance and taxonomic specificity.

The Potential Mechanisms of Ochratoxin A in Prostate Cancer Development: An Integrated Study Combining Network Toxicology, Machine Learning, and Molecular Docking.

Ochratoxin A (OTA), a prevalent food contaminant, has been proposed as a potential contributor to the development of prostate cancer, although its precise mechanisms remain unclear. This study employed a comprehensive approach that integrated network toxicology, machine learning, and molecular docking to clarify the role of OTA in prostate cancer. The findings indicated that OTA interacts with 364 targets related to prostate cancer, and machine learning was employed to identify five key molecular targets as priorities (ESR1, TP53, TNF, INS, and EGFR).

An innovative application of the holmium laser: a case report of hysteroscopic holmium laser proximal fallopian tubal occlusion.

Hysteroscopic proximal tubal occlusion is a treatment option prior to in vitro fertilization and embryo transfer (IVF-ET) in patients with a hydrosalpinx or salpingitis. The Holmium:YAG laser is well-suited for hysteroscopic procedures; however, its use in the treatment of salpingitis has not been previously reported. Here, we report a case of a 30-year-old female who presented to our hospital in 2018 after multiple failed embryo transfers.

Global landscape of PROTAC: Perspectives from patents, drug pipelines, clinical trials, and licensing transactions.

Proteolysis-targeting chimeras (PROTACs) represent an emerging therapeutic strategy that has experienced rapid growth since the first clinical proof-of-concept in 2020. Although numerous technical reviews exist, systematic analyses integrating multidimensional data from discovery through commercialization remain scarce. To fill this gap, this study analyzed comprehensive datasets from 2004 to 2024, covering 4840 patent applications, 170 drug pipelines, 123 clinical trials, and 44 licensing transactions, characterizing global trends, geographic distributions, collaborations, therapeutic landscapes, key targets, and stakeholder dynamics.

LncRNA-miRNA‒mRNA Network in Schizophrenia.

Schizophrenia (SCZ) is a severe mental disorder that significantly impacts the social functioning of patients and can reduce their life expectancy and quality of life. However, the specific causes of SCZ remain unknown, and the evidence indicates that long noncoding RNAs (lncRNAs) play critical roles in its pathogenesis. Analyzing lncRNA expression in peripheral blood samples from patients could reveal the biological mechanisms underlying the disease and help in the identification of biomarkers for early diagnosis and treatment.

Specific Recognition of Cysteine in a Mixed-Valence Bimetallic Organic Framework with Unique Channel Properties.

We have successfully designed and synthesized a mixed-valence bimetallic metal-organic framework (MOF), denoted ZnCoBTCHx, featuring periodic tubular and sinuous channels along with active sites provided by the ligands. This MOF demonstrates exceptional colorimetric selectivity toward cysteine (Cys). X-ray photoelectron spectroscopy, solid-state C NMR, UV-Vis absorption spectra, electrochemical analysis, and density functional theory calculations complementarily validate the valence state switching of cobalt centers in ZnCoBTCHx, the host-guest interactions and significant electron transfer between Cys and the framework.

Incorporating Stem Cell-Derived Small Extracellular Vesicles into a Multifunctional OHAMA-EPL Hydrogel Spray for Rapid Healing of Infected Skin Wounds.

Wound healing is a significant clinical concern that can be hindered by excessive inflammation, infection, and insufficient regeneration. Small extracellular vesicles (sEVs) derived from stem cells have been extensively studied for wound healing, but their efficient delivery and controllable release in the targeted tissues remain a technical challenge. A stem cell-derived sEVs incorporated dual-network hydrogel spray system is prepared for the rapid healing of infected full-thickness skin wounds for the first time.

Trends and emerging frontiers of photodynamic therapy for non-melanoma skin cancer (1979-October 2024): a bibliometric analysis.

Introduction: Photodynamic therapy (PDT) is a non-invasive treatment modality for non-melanoma skin cancer (NMSC). This study aims to use bibliometric analysis to explore the research trends and development in the field of PDT for NMSC, identifying key hotspots and emerging directions.

Methods: This study conducted a comprehensive literature search in the Web of Science Core Collection database and applied bibliometric analysis and visualization using tools such as VOSviewer, CiteSpace, and the R package "bibliometrix".

Whole transcriptome sequencing to uncover CircRNA expression patterns linked to schizophrenia pathogenesis.

Background: No objective diagnostic indicators have been identified for schizophrenia (SCZ) because of the complexity of its pathogenesis. Whole-transcriptome analysis may help identify early diagnostic markers. While circular RNAs (circRNAs) have been reported to be involved in the onset and development of many diseases, their association with SCZ remains unclear.

CCN5 negatively regulates TGF-β-induced endometriosis associated fibrosis through Wnt/β-catenin signaling via Smad3-dependent mechanism.

Background: Fibrogenesis is a common pathological feature of endometriotic lesions and contributes to the development of endometriosis-associated chronic pelvic pain and infertility. TGF-β is a critical factor in the induction of fibrogenesis; however, the underlying regulatory mechanisms of TGF-β-induced fibrosis in endometriosis remain unclear. In this study, we investigated the effects and mechanisms of CCN5 in regulating the progression of TGF-β-induced fibrosis in endometriosis.

Extremely early genomic events and temporal order of esophageal squamous cell carcinogenesis: Longitudinal self-comparison of progressors and non-progressors.

The current surveillance guideline of esophageal squamous cell carcinoma (ESCC) runs the risk of underestimation of early Lugol-unstained lesions (LULs), and extremely early genomic events in the carcinogenesis and their temporal order of occurrence remain unclear. Here, we performed whole-exome sequencing analyses of 148 biopsy samples obtained at different time points (with a median 4.6-year interval) from the same esophageal lesions of 74 asymptomatic subjects with LULs detected at community-based screening, of whom 33 individuals showed progression at the follow-up chromoendoscopy, while the other 41 did not.

Predicting Adverse Outcomes in Kidney Transplant Recipients Using an Interpretable Model Based on Shear-Wave Elastography.

Introduction: Shear-wave elastography (SWE) is a promising noninvasive technique for measuring renal fibrosis after transplantation. This study aimed to develop an interpretable model to predict allograft deterioration in kidney transplant recipients and evaluate the predictive ability of SWE features.

Methods: In this prospective cohort study, we performed SWE examinations on kidney transplant recipients at Renji Hospital between October 2020 and August 2023.

Human alveolar macrophage response to Mycobacterium tuberculosis: immune characteristics underlying large inter-individual variability.

Mycobacterium tuberculosis (M.tb) infection infects human alveolar macrophages (HAMs). In freshly isolated HAMs from 28 healthy adults, we observe large inter-individual differences in bacterial uptake and growth, with tenfold variation in M.

A digitally enabled home-based wound care program using the PEDALs model: a mixed-methods study protocol.

Background: Chronic wounds impose a significant burden on patients and caregivers because of complications such as pain, infection, and repeated trips to and from hospitals. These challenges are compounded by prolonged healing times and substantial socioeconomic costs, particularly in aging populations and resource-limited settings. The Digitally Enabled Home-Based Wound Care (DE-HBWC) program facilitates the organization of home visits by caregivers through electronic devices and internet platforms, enabling patients to easily receive medication changes and treatments, thus allowing patients to continue to reside in familiar surroundings while enjoying the same treatment as they would in a hospital.

An old spice with new tricks: Curcumin targets adenoma and colorectal cancer stem-like cells associated with poor survival outcomes.

The cost of cancer care globally is unsustainable and strategies to reduce the mounting burden of cancer are urgently needed. One approach is the use of preventive therapies to reduce cancer risk; dietary-derived compounds with good safety profiles represent a promising source of potential candidates but translating encouraging preclinical data to successful trials presents significant challenges. Development of curcumin, from the spice turmeric, as a preventive therapy for colorectal cancer (CRC) is hindered by poor understanding of its mechanism of action.

Novel insights showing SecA protein localization and distribution of phytoplasma in insect vector Empoasca paraparvipenis.

The Camptotheca acuminata witches-broom (CaWB-YNws) is one of the most severe diseases of C. acuminata and the insect vector is Empoasca paraparvipenis Zhang and Liu-CA (E. paraparvipenis-CA).

Mesenchymal Stem Cell-Derived Extracellular Vesicles Embedded in a Self-Adaptive Multifunctional Hydrogel for Rapid Healing of Infected Wounds.

Wound healing represents a major clinical concern, disrupted by cellular dysfunction due to infection, metabolic imbalance, and immune response, resulting in physical and economic burden to the patients. Despite the widespread recognition of mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) in wound healing research, ensuring their release and retention at the wound site and maximizing their therapeutic efficacy remain significant challenges. To address these issues, a self-adaptive multifunctional hydrogel is introduced that allows the self-adaptive release of MSC-EVs at the wound sites.

Interfacial Strength Between Porous Metal Acetabular Components and Augments.

Background: The use of porous metal acetabular components combined with augments is a potential approach for revision hip arthroplasty in cases of severe bone defects. However, the strength of the augment-acetabular cup interface has not been studied previously. This study evaluated the interfacial strength between acetabular cups and augments.

Metabolic reprogramming and therapeutic targeting in non-small cell lung cancer: emerging insights beyond the Warburg effect.

Non-small cell lung cancer (NSCLC) remains a leading cause of cancer-related mortality worldwide. Recent advancements have illuminated the intricate metabolic reprogramming that underpins NSCLC progression and resistance to therapy. Beyond the classical Warburg effect, emerging evidence highlights the pivotal roles of altered lipid metabolism, amino acid utilization, and the metabolic crosstalk within the tumor microenvironment (TME).

Demographic and clinical characteristics of children and adolescents with headache and/or dizziness and hemodynamic responses to head-up tilt test.

Background: Headache and dizziness are common symptoms in children and adolescents, but the causes of headache/dizziness in most pediatric patients are not clearly defined. We intended to investigate the demographic and clinical features of pediatric patients with headache and/or dizziness and responses to head-up tilt test (HUTT).

Methods: The demographic data and medical records of children and adolescents, with a primary complaint of headache and/or dizziness and undergoing HUTT between January 2001 and June 2023, were retrospectively reviewed and analyzed.

The Expression and Molecular Roles of MAMDC2 in MSS Colorectal Cancer with a High Tumor Stromal Ratio.

Colorectal cancer (CRC) heterogeneity is strongly influenced by molecular subtypes and tumor stroma interactions. The meprin/A5/PTPmu (MAM) domain, a conserved structural motif in transmembrane proteins, remains undercharacterized in CRC pathogenesis. We analyzed RNA-seq data from TCGA-COAD to evaluate MAM domain gene expression.