Therapeutic Targets and Immune Mechanisms of Yinghuang Decoction in Sepsis: Integrating Network Pharmacology, Molecular Docking, and Pharmacokinetic Approaches.

Background: Yinghuang Decoction is an herbal formula that is used for the treatment of sepsis. This study used network pharmacology and molecular docking methods to explore the potential mechanism of Yinghuang Decoction against sepsis.

Methods: The active ingredients, target genes, and sepsis-related differentially expressed genes (DEGs) were acquired from the public database.

Crystal structure and ligand binding of a sigma-class glutathione S-transferase associated with cross-resistance in a specialist herbivore.

Understanding the molecular mechanisms underlying insect adaptation is critical for elucidating the evolution of pesticide resistance and improving pest management strategies. While host plant preadaptation has been proposed to facilitate insecticide resistance, direct evidence remains limited. Here, we investigated a sigma-class glutathione S-transferase (GST), LdGSTs2, in the Colorado potato beetle (Leptinotarsa decemlineata), a major agricultural pest.

CRL: An Efficient Autonomous Exploration Framework for Large-Scale Environments With Contrastive-Driven Reinforcement Learning.

Autonomous exploration in large-scale environments is impeded by two critical challenges, namely, suboptimal viewpoint selection resulting from inadequate feature extraction and the continuously rising computational costs as the environment expands. Existing methods struggle to simultaneously tackle these dual challenges within cohesive frameworks. In response, we present an efficient autonomous exploration framework with contrastive-driven reinforcement learning.

Development and validation of a nomogram-based predictive model for recurrence risk of uterine leiomyoma after myomectomy.

Uterine fibroids are common among women of reproductive age and often recur after treatment. Accurate recurrence prediction is essential for guiding clinical decisions, yet existing models remain inadequate. This study aimed to develop a nomogram based on Least Absolute Shrinkage and Selection Operator (LASSO) regression to estimate recurrence risk after myomectomy.

Silver-Conjugated-Polyarginine Anchored Cotton Fabrics Exhibit Durable and Efficient Antimicrobial Properties.

Due to the hierarchical alignment of cellulose fibrils, cotton fabrics used in clothing possess excellent moisture and thermal management properties. However, this property can retain sweat and sebum secreted from human skin, which may harbor harmful microorganisms and affect human health. In addition, the abuse of antibiotics leads to the emergence of drug-resistant pathogens and cross infections, which require new strategies and antibacterial materials to control this situation.

Homeostatic remodeling platform for reprogramming Iron Intraspatial orientation to reverse liver fibrosis.

Chronic alcohol consumption disrupts iron homeostasis, leading to iron overload in liver parenchymal cells and activation of hepatic stellate cells, ultimately contributing to the development of alcoholic liver fibrosis. Due to the differing iron requirements of these two cell types, current treatment strategies often fail to effectively restore iron balance in the liver and may even exacerbate disease progression. To address this challenge, we developed a targeting-intelligent regulation platform composed of iron-depleting micelles (Met/Gal) and iron-redirecting micelles (Sor/DVA) for the treatment of alcoholic liver fibrosis and other disorders associated with iron dysregulation.

CT-Based radiomics and deep learning for the preoperative prediction of peritoneal metastasis in ovarian cancers.

Objectives: To develop a CT-based deep learning radiomics nomogram (DLRN) for the preoperative prediction of peritoneal metastasis (PM) in patients with ovarian cancer (OC).

Methods: A total of 296 patients with OCs were randomly divided into training dataset (N = 207) and test dataset (N = 89). The radiomics features and DL features were extracted from CT images of each patient.

Co-Occurrence of Depression, Anxiety, and Burnout: A Cross-Sectional Network Analysis.

Introduction: Discrepancies persist regarding burnout-depression-anxiety relationships in health care workers (HCWs), hindering interventions. This cross-sectional study developed a symptom-level network model to clarify their interconnections.

Methods: Nine hundred ninety-two HCWs completed online surveys assessing depression, anxiety, and burnout symptoms.

Size and Structure-Dependent Molecular Fingerprint Transformation of Microplastic-Derived Dissolved Organic Matter in Sunlit Seawater: Implication for Marine Carbon Cycles.

Microplastics (MPs) widely pollute marine environments, where the release of MPs-derived dissolved organic matter (MPs-DOM) induced by UV irradiation has been widely documented. However, the effect of MPs' inherent properties on photochemical transformation of MPs-DOM and its implication for oceanic carbon cycles remain poorly understood. Herein, we conducted 180 day artificial weathering experiments under sunlit seawater using polyethylene (PE) and polypropylene (PP) with different sizes as models, investigating the temporally dynamic features of MPs-DOM.

In vivo Multimodal Magnetic Particle Imaging for Early Detection of Ischemic Stroke in Tree Shrews.

Purpose: Ischemic stroke is a significant threat to human life and health, and timely diagnosis is essential for improving patient outcomes. Magnetic Particle Imaging (MPI), as an emerging high-sensitivity imaging technology, holds significant potential for the diagnosis of ischemic stroke. It is necessary to conduct multimodal MPI research based on the characteristics of the animal model and the detection needs of ischemic stroke.

Huangqi Guizhi Wuwu decoction ameliorates myocardial damage in ovariectomized rats by regulating the structure and function of myocardial microvessels and intestinal flora.

Huangqi Guizhi Wuwu decoction (HGWD) is commonly used to treat cardiovascular diseases (CVDs), and HGWD could regulate intestinal flora and myocardial microvessels, this study aimed to examine whether HGWD could ameliorate myocardial damage in ovariectomized (OVX) rats by regulating the structure and function of myocardial microvessels and intestinal flora. A model of OVX rats was created, and histological examination through hematoxylin and eosin staining alongside TEM was employed to assess the microstructural lesions present in the myocardium. Myocardial microvasculature density was assessed using immunofluorescent staining.

Magnetically induced magnetosome chain (MAGiC): A biogenic magnetic-particle-imaging tracer with high performance and navigability.

Magnetic particle imaging (MPI) enables real-time, sensitive, and quantitative visualization of tracer distribution, augmenting the capability of in vivo imaging technologies. Previous MPI tracer research has predominantly focused on superparamagnetic nanoparticles, whose suboptimal sigmoidal magnetization curves limit their spatial resolution. Here, we introduce the magnetically induced magnetosome chain (MAGiC), a superferromagnetic MPI tracer, demonstrating a 25-fold improvement in resolution in the excitation direction and an order of magnitude improvement in signal intensity compared to the commercial tracer VivoTrax+.

The Effect of the AQP1 Gene Knockout on the Diversity, Composition and Function of Gut Microbiota in Mice with Heart Failure.

Introduction: This study aims to elucidate the impact of AQP1 on cardiac function and the intestinal microbiota in mice with chronic heart failure and to further investigate the broad effects of AQP1 on the gut microbiota composition in these mice.

Methods: AQP1 knockout mice were used as the experimental group, with wild-type mice serving as the control group. The study evaluated the effects of AQP1 on various physiological parameters, including blood pressure, heart rate, cardiac function, cardiac color Doppler ultrasound, and 24 h urine collection.

Macrophages form dendrite-like pseudopods to enhance bacterial ingestion.

Macrophages are critical innate immune cells that exhibit remarkable adaptability during pathogen infections. However, the relationship between their morphological plasticity and physiological functions remains largely elusive. Here, we discovered an unprecedented paradigm of macrophage adaptation within a few hours upon severe Gram-negative bacterial infections, characterized by the formation of dendrite-like pseudopods (DLPs).

Identification of a mutation in a Chinese family with MYH9-related disease.

Objectives: -related disease (-RD) is a congenital bleeding disorder characterized by thrombocytopenia, platelet macrocytosis, inclusion bodies in neutrophils.The aim of this study was to investigate a Chinese family with -RD and to identity potential mutations of the This investigation will elucidate the molecular mechanisms involved in disease pathogenesis.

Methods: Whole exome sequencing (WES) followed by Sanger sequencing was conducted on all family members.

Functional connectivity network based on scalp electroencephalogram in juvenile myoclonic epilepsy.

Background: Advances in neuroimaging technology have identified epilepsy as a disorder of brain network dysfunction. Previous research has confirmed alterations in the brain functional network of patients with juvenile myoclonic epilepsy (JME). However, studies investigating brain network changes in JME patients under different intervention conditions remain scarce.

Single-cell sequencing combined with spatial transcriptomics reveals the characteristics of follicle-targeted inflammation patterns in primary cicatricial alopecia.

Background: Primary cicatricial alopecia (PCA) causes irreversible hair loss due to immune-mediated hair follicle destruction. This study investigates follicle-targeted inflammation in lichen planopilaris (LPP), a major PCA subtype, to identify therapeutic targets.

Methods: Scalp samples from LPP, localized scleroderma (LS), and controls were analyzed using single-cell RNA sequencing and spatial transcriptomics.

Methane filtration and metabolic cooperation of microbial communities in cold seep water columns from South China Sea.

Microbes in cold seep water columns are essential for methane sequestration and biogeochemical cycling, yet their structures and ecological functions, particularly at the bottom water interface (BWI), are poorly understood. Here, we performed metagenomic analyses to explore the microbial biodiversity and functions at the F-site cold seep in the South China Sea. Functional stratification revealed that photosynthetic autotrophs dominate surface zones, heterotrophs are prevalent in mesopelagic zones, and chemosynthetic bacteria are abundant at the BWI.

Plastispheres as unique niches for promoting turnover of dissolved organic matter in aquatic environments.

Plastic debris has been found to be ubiquitous in aquatic ecosystems, providing a novel substrate for biofilm formation called the plastisphere. Existing surveys mainly focus on the fact that this novel niche serves as a reservoir for environmental contaminants, whereas the potential role of the plastisphere in affecting biogeochemical cycles of aquatic dissolved organic matter (DOM), one of the largest pools of active carbon, remain largely unknown. Herein, we conducted in-situ field incubation and lab-scale microcosm experiments using river water as background, investigating the variation and underlying mechanisms of riverine DOM composition under plastisphere incubation at molecular level.

E-Cigarette Smoke Exposure Elevates Renal MR Expression and Induces BP Elevation.

Background: E-cigarette use increases the risk of blood pressure (BP) elevation in users, though the underlying mechanisms remain unclear. The mineralocorticoid receptor (MR) is known to play an important role in the regulation of renal electrolyte balance and BP, and is protected by 11β-HSD2 (11β-hydroxysteroid dehydrogenase type 2). However, little is known about the effects of renal MR on e-cigarette-induced BP elevation.

Molecular fingerprints of dissolved organic matter leached from microplastics over prolonged photochemical aging: Implications for aquatic carbon cycling.

Microplastics are well-known emerging pollutants in aquatic environments, increasingly shown to release significant amounts of non-natural dissolved organic matter (DOM) into water systems. However, the molecular composition of DOM leached from different types of microplastics after long-term photochemical aging, and their impact on the aquatic carbon cycle, remain poorly understood. The potential presence and contribution of microplastic-derived DOM to the river-to-ocean continuum on a large scale have yet to be well-established.

Mitochondrial thermal proteome profiling reveals aberrant activation of pyruvate dehydrogenase upon cuproptosis.

Cuproptosis, a copper-induced form of regulated cell death, holds therapeutic promise in cancer but remains mechanistically unclear. We developed Mito-TPCA, a mitochondrial thermal proximity coaggregation strategy combining enzyme-catalyzed proteome labeling with thermal profiling, to map mitochondrial protein-protein interaction dynamics during cuproptosis. This approach revealed that copper disrupts the association of pyruvate dehydrogenase kinases (PDKs) with the pyruvate dehydrogenase (PDH) complex by targeting lipoyl domains, triggering PDH dephosphorylation and aberrant activation.

Nosocomial transmission, adaption and clinical outcomes of carbapenem-resistant hypervirulent Klebsiella pneumoniae.

Background: Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKp) poses a significant threat in healthcare settings. This study investigates the nosocomial transmission dynamics, adaptive phenotypes, and clinical outcomes of CR-HvKp with different evolutionary patterns.

Methods: A genomic analysis of 2,002 Klebsiella pneumoniae isolates collected from a major teaching hospital in China was performed to identify convergent isolates.

Amylopectin branch trimming and biosynthesis elucidated by the rice isoamylase ISA1-ISA2 heterocomplex.

Amylopectin, the primary form of starch in plant leaves, seeds and tubers, features a tree-like architecture with branched glucose chains. Excess branches result in the formation of soluble phytoglycogen instead of starch granules. In higher plants and green algae, the debranching enzyme isoamylase ISA1 forms either homomultimer or hetero-multimer with ISA2 to facilitate branch trimming and starch granule formation, but the molecular basis remains largely unknown.

Down-regulation of neuregulin2 (NRG2) following spinal cord injury in C57BL/6 mice: Its implications in therapeutic potential.

Objectives: This study aims to elucidate the alterations in neuregulin 2 (NRG2) and its receptor ErbB4 following spinal cord injury (SCI), as well as to investigate the neuroprotective mechanisms of NRG2 in neurons.

Materials And Methods: Dataset GSE93561 was analyzed to verify the changes of NRG2-ErbB4 signaling pathway in mice following SCI. The levels of Iba-1 and NRG2 were analyzed by immunohistochemistry, and NRG2 and pErbB4 protein levels were detected by western blot.