Analysis of the Differences in Rhizosphere Microbial Communities and Pathogen Adaptability in Chili Root Rot Disease Between Continuous Cropping and Rotation Cropping Systems.

In chili cultivation, obstacles to continuous cropping significantly compromise crop yield and soil health, whereas crop rotation can enhance the microbial environment of the soil and reduce disease incidence. However, its effects on the diversity of rhizosphere soil microbial communities are not clear. In this study, we analyzed the composition and characteristics of rhizosphere soil microbial communities under chili continuous cropping (CC) and chili-cotton crop rotation (CR) using high-throughput sequencing technology.

Integrative identification of key genes governing wilt resistance in using machine learning and WGCNA.

Introduction: wilt, caused by , is one of the most devastating diseases affecting global cotton () production. Given the limited effectiveness of chemical control measures and the polygenic nature of resistance, elucidating the key genetic determinants is imperative for the development of resistant cultivars. In this study, we aimed to dissect the temporal transcriptional dynamics and regulatory mechanisms underlying response to infection.

6-Hydroxynicotinic Acid From Cucumis melo Inhibits Prehaustorium Formation in Phelipanche aegyptiaca via Disruption of Auxin Signalling Pathway.

Phelipanche aegyptiaca, a root holoparasitic weed, severely threatens agricultural productivity due to its detrimental effects. This species relies on a specialised organ, namely the haustorium, to extract nutrients from its host plants. The germination and haustorium formation of P.

Decoding the pancreatic cancer microenvironment: The multifaceted regulation of microRNAs.

Pancreatic cancer (PC) is an extremely deadly type of cancer, and the 5-year survival rate remains less than 10%. The tumour microenvironment (TME) affects the occurrence, progression and treatment outcomes of PC. MicroRNAs (miRNAs) are essential to regulate PC TME.

RIPK4 promotes epidermal differentiation through phase separation and activation of LATS1/2.

The basal layer progenitors establish a stratified epidermis through asymmetric division and differentiation. Inactivating mutations of Receptor-interacting serine/threonine kinase 4 (RIPK4) cause human developmental syndromes characterized by defective epidermal differentiation. While the Hippo pathway is crucial in limiting organ size, emerging evidence suggests that it also plays additional roles in differentiation.

Lactylation: the regulatory code of cellular life activity and a barometer of diseases.

Lactylation is a novel post-translational modification of proteins, which has attracted extensive attention since its discovery. Lactylation takes lactate, a common metabolite, as its substrate and mediates the modification under the action of lactyltransferases. Although lactylation modification was initially found to undergo in histones, subsequent studies have shown that this novel modification is not limited to specific protein classes, and can undergo in both histone and non-histone proteins.

IAA-Mediated Haustorium Formation in : Transcriptional Insights and Anti-Parasitic Strategies.

is an obligate root-parasitic weed that parasitizes crop roots, threatening the safety of agricultural production. However, the molecular mechanisms underlying the formation of haustorium remain largely unclear. Here, we employed transcriptomics to investigate the molecular events in haustorium formation induced by indole-3-acetic acid.

Secretory Proteins Are Involved in the Parasitism of Melon by During the Attachment Stage.

Parasitic plants represent a significant challenge in global agriculture, with Broomrape (/ spp.) being a notable example of a holoparasitic species that targets the roots of host plants. This study employed comparative transcriptomics to investigate the mechanisms underlying the parasitism of on melon, focusing on both resistant and susceptible interactions.

Isolation of Haustorium Protoplasts Optimized by Orthogonal Design for Transient Gene Expression in .

The efficient protoplast transient transformation system in plants is an important tool to study gene expression, metabolic pathways, and various mutagenic parameters, but it has not been established in . As a root parasitic weed that endangers the growth of 29 species of plants in 12 families around the world, there is still no good control method for . Even the parasitic mechanisms of and the related genes regulating parasitism are not yet understood.

Jasmonate ZIM Domain Protein () Gene Increases Resistance to in Tomato.

Pers. is a holoparasitic plant that severely reduces tomato ( L.) production in China.

The RNF214-TEAD-YAP signaling axis promotes hepatocellular carcinoma progression via TEAD ubiquitylation.

RNF214 is an understudied ubiquitin ligase with little knowledge of its biological functions or protein substrates. Here we show that the TEAD transcription factors in the Hippo pathway are substrates of RNF214. RNF214 induces non-proteolytic ubiquitylation at a conserved lysine residue of TEADs, enhances interactions between TEADs and YAP, and promotes transactivation of the downstream genes of the Hippo signaling.

Integrated Transcriptome and Proteome Analysis Reveals That Cell Wall Activity Affects Parasitism.

can infect many crops, causing large agricultural production losses. It is important to study the parasitism mechanism of to control its harm. In this experiment, the HY13M and TE9M from Tacheng Prefecture and Hami City in Xinjiang, respectively, were used to analyze the parasitical mechanism of by means of transcriptome and proteome analyses.

Featured lncRNA-based signature for discriminating prognosis and progression of hepatocellular carcinoma.

Long non-coding RNAs (lncRNAs) have been implicated in carcinogenesis and progression of hepatocellular carcinoma (HCC). This study aimed to identify a robust lncRNA signature for predicting the survival of HCC patients. We performed an integrated analysis of the lncRNA expression profiling in The Cancer Genome Atlas (TCGA)-liver hepatocellular carcinoma database to identify the prognosis-related lncRNA for the HCC.

A new method: Characterize and quantify biofilm wrinkles by UNet and Sholl Analysis.

The wrinkles on the biofilm contain a lot of information about biofilm growth, so it is essential to characterize and quantify these wrinkles from the original microscopic images to discover more rules governing the biofilm morphology evolution. However, the existing methods to extract the wrinkles are time-consuming, error-prone, and require manual calibration. We propose a new system: using a deep learning method - UNet to identify the biofilm wrinkles in the original experimental images, which can achieve fast and accurate extraction of wrinkles on biofilms.

LATS2 condensates organize signalosomes for Hippo pathway signal transduction.

Biomolecular condensates have been proposed to mediate cellular signaling transduction. However, the mechanism and functional consequences of signal condensates are not well understood. Here we report that LATS2, the core kinase of the Hippo pathway, responds to F-actin cytoskeleton reduction and forms condensates.

A chaperone-like function of FUS ensures TAZ condensate dynamics and transcriptional activation.

The Hippo pathway has important roles in organ development, tissue homeostasis and tumour growth. Its downstream effector TAZ is a transcriptional coactivator that promotes target gene expression through the formation of biomolecular condensates. However, the mechanisms that regulate the biophysical properties of TAZ condensates to enable Hippo signalling are not well understood.

A Method of Human-Like Compliant Assembly Based on Variable Admittance Control for Space Maintenance.

On-orbit assembly has become a crucial aspect of space operations, where the manipulator frequently and directly interacts with objects in a complex assembly process. The traditional manipulator control has limitations in adapting to diverse assembly tasks and is vulnerable to vibration, leading to assembly failure. To address this issue, we propose a human-like variable admittance control method based on the variable damping characteristics of the human arm.

Detection of Secreted Effector Proteins from During Invasion of Melon Roots.

Parasites can interact with their host plants through the induction and delivery of secreted effector proteins that facilitate plant colonization by decomposing plant cell walls and inhibiting plant immune response to weaken the defense ability of the host. Yet effectors mediating parasitic plant-host interactions are poorly understood. is an obligate root parasite plant causing severe yield and economic losses in agricultural fields worldwide.

Disulfiram with Cu alleviates dextran sulfate sodium-induced ulcerative colitis in mice.

Disulfiram (DSF), a Food and Drug Administration (FDA)-approved drug for chronic alcohol addiction, has anti-inflammatory effects that help prevent various cancers, and Cu can enhance the effects of DSF. Inflammatory bowel diseases (IBD) are characterized by chronic or recurrent relapsing gastrointestinal inflammation. Many drugs targeting the immune responses of IBD have been developed, but their application has many problems, including side effects and high costs.

Isorhamnetin attenuates the proliferation, invasion, migration and fibrosis of keloid fibroblasts by targeting S1PR1.

Isorhamnetin (IH) is a type of flavonoid with multiple biological activities, including cardioprotective, antitumor, anti-inflammatory and antioxidant activities. However, the role and potential mechanism of IH in keloids are still not completely understood. The aim of the present study was to explore how IH affects keloid progression.

Phenotypic and histological analyses on the resistance of melon to .

Melon ( L.) is an economically important crop in Xinjiang, China, but its production is constrained by the parasitic plant that attaches to the roots of many crops and causes severe stunting and loss of yield. Rhizotron, pot, and field experiments were employed to evaluate the resistance of 27 melon cultivars to .