RIPK4 promotes epidermal differentiation through phase separation and activation of LATS1/2.

The basal layer progenitors establish a stratified epidermis through asymmetric division and differentiation. Inactivating mutations of Receptor-interacting serine/threonine kinase 4 (RIPK4) cause human developmental syndromes characterized by defective epidermal differentiation. While the Hippo pathway is crucial in limiting organ size, emerging evidence suggests that it also plays additional roles in differentiation.

scCDC: a computational method for gene-specific contamination detection and correction in single-cell and single-nucleus RNA-seq data.

In droplet-based single-cell and single-nucleus RNA-seq assays, systematic contamination of ambient RNA molecules biases the quantification of gene expression levels. Existing methods correct the contamination for all genes globally. However, there lacks specific evaluation of correction efficacy for varying contamination levels.

Decoding leukemia at the single-cell level: clonal architecture, classification, microenvironment, and drug resistance.

Leukemias are refractory hematological malignancies, characterized by marked intrinsic heterogeneity which poses significant obstacles to effective treatment. However, traditional bulk sequencing techniques have not been able to effectively unravel the heterogeneity among individual tumor cells. With the emergence of single-cell sequencing technology, it has bestowed upon us an unprecedented resolution to comprehend the mechanisms underlying leukemogenesis and drug resistance across various levels, including the genome, epigenome, transcriptome and proteome.