Pediatric sequential organ failure assessment for predicting outcomes in ECMO-bridged pediatric heart transplant recipients: experience from the largest pediatric heart transplant center in China.

Objective: Advanced heart failure in children sometimes requires mechanical circulatory support as a bridge to transplantation, with extracorporeal membrane oxygenation (ECMO) remaining a critical option despite its associated risks. The pediatric Sequential Organ Failure Assessment (pSOFA) may have potential in evaluating prognosis in ECMO-bridged candidates.

Methods: 188 Children underwent orthotopic heart transplantation in Union hospital, Tongji Medical College, Huazhong University of Science and Technology, between January 2018 and April 2025 were studied retrospectively, with 24 received ECMO assistance as a bridge to transplant.

Based on two coconut ( L.) genome-wide investigation of NODULE-INCEPTION-like protein family: evolution and expression profiles during development and stress.

NODULE-INCEPTION-Like Protein (NLP) is a plant-specific transcription factor that plays a crucial role in nitrate uptake and stress responses. However, studies of gene family in coconut are lacking. In this study, 7 genes in the and 6 genes in coconut varities were identified.

Genome-Wide Identification and Expression Assessment for the Gene Family Within Sweet Potato Under Phosphorus Deficiency Stress.

Hainan's unique climate significantly contributes to soil acidification, causing phosphorus fixation into insoluble compounds, leading to phosphorus deficiency and reduced yield in sweet potatoes. The family, a group of trans-membrane phosphate transporters, is crucial for phosphate transport, distribution, and homeostasis regulation. Two genes, and , were first identified in sweet potato, and a phylogenetic analysis of 46 species showed high conservation of the gene family throughout plant evolution.

PP2A Attenuates Thoracic Aneurysm and Dissection in Mouse Models of Marfan Syndrome.

Background: Recent studies show that hyperactivation of mTOR (mammalian target of rapamycin) signaling plays a causal role in the development of thoracic aortic aneurysm and dissection. Modulation of PP2A (protein phosphatase 2A) activity has been shown to be of significant therapeutic value. In light of the effects that PP2A can exert on the mTOR pathway, we hypothesized that PP2A activation by small-molecule activators of PP2A could mitigate AA progression in Marfan syndrome (MFS).

Te L-subshell x-ray emission induced by lower energy H ions.

The L-shell x-ray emission of tellurium induced by H ions impact is investigated in an energy range of 150-300 keV. The blue shifts of various L-subshell x rays and the enhancement of the relative intensity ratios of Lι, Lβ to Lα x ray are observed and interpreted by the multiple ionization of outer-shell electrons. The new experimental x-ray production cross sections, which almost correspond to those of protons with half of the original energy, are extracted and compared with various theoretical estimations.

Clinical Outcomes and Risk Factors of Heart Transplantation Patients Experiencing Gastrointestinal Bleeding.

Gastrointestinal bleeding (GIB) after heart transplantation (HT) remains a significant clinical issue. This study aimed to explore the incidence, trends, outcomes, and clinical predictors of GIB in HT patients. Adult patients who underwent HT between 2015 and 2021 at Union Hospital were recruited and divided into two groups based on the presence or absence of postoperative GIB.

Insight into the potentiality of nano zero-valent iron on enhancing the nitrite accumulation and phosphorus removal performance of endogenous partial denitrification systems.

Endogenous partial denitrification (EPD) has drawn a lot of interest due to its abundant nitrite (NO-N) accumulation capacity. However, the poor phosphate (PO-P) removal rate of EPD restricts its promotion and application. In this study, the potentiality of various nano zero-valent iron (nZVI) concentrations (0, 20, 40, and 80 mg/L) on NO-N accumulation and PO-P removal in EPD systems had been investigated.

Protein Phosphatase 2A Activation Promotes Heart Transplant Acceptance in Mice.

Background: Although heart transplantation is the definitive treatment for heart failure in eligible patients, both acute and chronic transplant rejection frequently occur. Protein phosphatase 2A (PP2A) activity is critical in maintaining tissue and organ homeostasis. In this study, we evaluated the effect of a novel class of small molecule activators of PP2A (SMAPs) on allograft rejection in a mouse heterotopic heart transplantation model.

RING-type E3 ligase BnaJUL1 ubiquitinates and degrades BnaTBCC1 to regulate drought tolerance in Brassica napus L.

Drought stress poses a persistent threat to field crops and significantly limits global agricultural productivity. Plants employ ubiquitin-dependent degradation as a crucial post-translational regulatory mechanism to swiftly adapt to changing environmental conditions. JUL1 is a RING-type E3 ligase related to drought stress in Arabidopsis.

Genome-wide identification and expression analysis of phenylalanine ammonia-lyase (PAL) family in rapeseed (Brassica napus L.).

Background: Phenylalanine ammonia-lyase (PAL), as a key enzyme in the phenylalanine metabolism pathway in plants, plays an important role in the response to environmental stress. However, the PAL family responding to abiotic stress has not been fully characterized in rapeseed.

Results: In this study, we conducted a genome-wide study of PAL family, and analyzed their gene structure, gene duplication, conserved motifs, cis-acting elements and response to stress treatment.

The advance of CCN3 in fibrosis.

The extracellular matrix (ECM) is comprised of various extracellular macromolecules, including collagen, enzymes, and glycoproteins, which offer structural and biochemical support to neighboring cells. After tissue injury, extracellular matrix proteins deposit in the damaged tissue to promote tissue healing. However, an imbalance between ECM production and degradation can result in excessive deposition, leading to fibrosis and subsequent organ dysfunction.

Target Density Effects on Charge Transfer of Laser-Accelerated Carbon Ions in Dense Plasma.

We report on charge state measurements of laser-accelerated carbon ions in the energy range of several MeV penetrating a dense partially ionized plasma. The plasma was generated by irradiation of a foam target with laser-induced hohlraum radiation in the soft x-ray regime. We use the tricellulose acetate (C_{9}H_{16}O_{8}) foam of 2  mg/cm^{3} density and 1 mm interaction length as target material.

Myeloid CCN3 protects against aortic valve calcification.

Background: Cellular communication network factor 3 (CCN3) has been implicated in the regulation of osteoblast differentiation. However, it is not known if CCN3 can regulate valvular calcification. While macrophages have been shown to regulate valvular calcification, the molecular and cellular mechanisms of this process remain poorly understood.

CCN2 deficiency in smooth muscle cells triggers cell reprogramming and aggravates aneurysm development.

Vascular smooth muscle cell (SMC) phenotypic switching is widely recognized as a key mechanism responsible for the pathogenesis of several aortic diseases, such as aortic aneurysm. Cellular communication network factor 2 (CCN2), often upregulated in human pathologies and animal disease models, exerts myriad context-dependent biological functions. However, current understanding of the role of SMC-CCN2 in SMC phenotypic switching and its function in the pathology of abdominal aortic aneurysm (AAA) is lacking.

Quantitative trait locus mapping and improved resistance to sclerotinia stem rot in a backbone parent of rapeseed ( L.).

There are three main challenges to improving sclerotinia stem rot (SSR) resistance in rapeseed ( L.). First, breeding materials such as the backbone parents have not been extensively investigated, making the findings of previous studies difficult to directly implement.

Au L-shell x-ray emission induced by 154.3-423.9 MeV/u C ions.

The L-shell x-ray emissions of gold are investigated for the bombardment of high energy C ions in the high energy region of 154.3-423.9 MeV/u.

Multiple ionization of iodine for 2.5-5.0 MeV I ions impacting on Fe target.

The L-shell X-ray emissions of iodine are investigated as a function of the incident energy for I ions impacting on Fe target in the energy region near the Bohr velocity. Six distinct L-subshell X-rays, Lι, Lα, Lβ, Lβ, Lγ and Lγ, are observed. Compared to the atomic data, the energy of the experimental X ray shifts to the higher energy side.

Protein Phosphatase 2A Deficiency in Macrophages Increases Foam Cell Formation and Accelerates Atherosclerotic Lesion Development.

Protein phosphatase 2A (PP2A), a crucial serine/threonine phosphatase, has recently been reported to play an important role in cardiovascular disease. Previous studies have hinted that PP2A is involved in atherosclerosis formation, but the associated mechanisms remain poorly understood. In this study, we investigate the role of PP2A in the pathogenesis of atherosclerosis.

BnaC7.ROT3, the causal gene of cqSL-C7, mediates silique length by affecting cell elongation in Brassica napus.

Siliques are a major carbohydrate source of energy for later seed development in rapeseed (Brassica napus). Thus, silique length has received great attention from breeders. We previously detected a novel quantitative trait locus cqSL-C7 that controls silique length in B.

Allosteric activation of PP2A inhibits experimental abdominal aortic aneurysm.

Although extremely important, the molecular mechanisms that govern aortic aneurysm (AA) formation and progression are still poorly understood. This deficit represents a critical roadblock toward the development of effective pharmaceutical therapies for the treatment of AA. While dysregulation of protein phosphatase 2A (PP2A) is thought to play a role in cardiovascular disease, its role in aortic aneurysm is unknown.

Observation of a high degree of stopping for laser-accelerated intense proton beams in dense ionized matter.

Intense particle beams generated from the interaction of ultrahigh intensity lasers with sample foils provide options in radiography, high-yield neutron sources, high-energy-density-matter generation, and ion fast ignition. An accurate understanding of beam transportation behavior in dense matter is crucial for all these applications. Here we report the experimental evidence on one order of magnitude enhancement of intense laser-accelerated proton beam stopping in dense ionized matter, in comparison with the current-widely used models describing individual ion stopping in matter.

The natural compound andrographolide inhibits human aortic valve interstitial cell calcification via the NF-kappa B/Akt/ERK pathway.

Calcific aortic valve disease (CAVD) is caused by valve interstitial cells (VICs) initiating the thickening and calcification of valve leaflets. The present study aimed to investigate whether andrographolide (AGP) could attenuate the calcification of human valve interstitial cells (hVICs). hVICs stimulated by osteoblastic medium (OM) were treated with or without AGP.

Fine mapping and candidate gene analysis of a seed glucosinolate content QTL, qGSL-C2, in rapeseed (Brassica napus L.).

QTL mapping and candidate gene analysis indicate that allelic variations in BnaC2.MYB28 resulted from homeologous exchange and determine difference in seed glucosinolate content. A low seed glucosinolate content has long been an important breeding objective in rapeseed improvement.

Multiple ionization state of Ar ions during collisions near the Bohr velocity.

In order to clarify the mechanism and the influence of the initial charge state and target atomic parameters for the formation of L-shell multiple ionization state of Ar ions produced by the collisions near the Bohr velocity, the k-shell x-ray emission of Ar is measured for 1.2 MeV Ar ions impacting on V target and 3 MeV Ar ions interacting with selected targets (Z = 23, 26, 27, 28, 29, 30). It is found that the measured Ar Kα and Kβ x-ray energies shift to the high energy side, and the relative intensity ratios of Kβ/Kα are enlarged than the atomic data, owing to the presence of out-shell multiple vacancies.