Molecular Layer Atrophy Mediates the Association of Cumulative Blood Pressure Exposure With Cognitive Decline.

Background: High blood pressure (BP) is a known risk factor for cognitive decline and dementia, but the underlying mechanisms are largely unknown. We investigated the associations of cumulative BP exposure with hippocampal subfield volume and cognitive function and determined whether hippocampal subfield atrophy mediates the association between cumulative BP exposure and cognitive decline.

Methods: Between December 2020 and March 2023, participants were prospectively included from the Kailuan study.

Molecular insights into ZER1 recognition of N-terminal residue mutations.

N-terminal glycine (Gly/N-degron), as a degradation signal, can be recognized by specific E3 ubiquitin ligases and plays a crucial role in protein degradation and cellular homeostasis. As a substrate receptor in the Cullin 2-RING E3 ligase complex, ZER1 mediates protein degradation the Gly/N-degron pathway by recognizing N-terminal glycine and other small residues. This study employed all-atom molecular dynamics (MD) simulations and binding free energy calculations to explore ZER1's recognition of the wild-type peptide GFLHVGQD (WT) and its N-terminal mutants (G1S, G1A, G1T, and G1C).

Low-Intensity pulsed ultrasound inhibits the ROS/NF-κB signaling pathway and improves inflammation in ovarian granulosa cells of PCOS rats.

Polycystic Ovary Syndrome (PCOS) is a prevalent endocrine and metabolic condition among women of reproductive age, frequently associated with oxidative stress (OS) and chronic low-grade inflammation. Low-Intensity pulsed ultrasound (LIPUS) is a safe, non-invasive physical therapy method widely used in promoting fracture healing, soft tissue repair, and reducing inflammation. This study explored the improving effect of LIPUS on ovarian function in PCOS rat models induced by letrozole and a high-fat diet.

Microstructural and Mechanical Property Variations in 316L Stainless Steel Fabricated by Laser Powder Bed Fusion Under High-Density Processing Conditions.

It has become a trend to precisely control the additive manufacturing process parameters within the high-density process window to obtain high-performance metal parts. However, there are few reports on this topic currently, leaving this research without sufficient references. This study took 316L austenitic stainless steel as a case study.

IF-MMCL: an individual focused network with multi-view and multi-modal contrastive learning for cross-subject emotion recognition.

Electroencephalography (EEG) usage in emotion recognition has garnered significant interest in brain-computer interface (BCI) research. Nevertheless, in order to develop an effective model for emotion identification, features need to be extracted from EEG data in terms of multi-view. In order to tackle the problems of multi-feature interaction and domain adaptation, we suggest an innovative network, IF-MMCL, which leverages multi-modal data in multi-view representation and integrates an individual focused network.

Uncut Roux-en-Y for very small remnant stomach in gastric cancer: surgical advantages and nutritional outcomes.

Background: The optimal reconstruction method after laparoscopic distal gastrectomy (LDG) for gastric cancer remains controversial. This study evaluates the novel approach of subtotal gastrectomy with preservation of a small remnant stomach (SRS) combined with uncut Roux-en-Y (URY) reconstruction, focusing on surgical feasibility, complications, and nutritional outcomes.

Methods: A retrospective analysis of patients undergoing LDG (2017-2023) was conducted.

Pyrroloquinoline quinone activates lactate and inhibits NLRP3 to improve ovarian reserve in premature ovarian insufficiency.

Research Question: Can the use of pyrroloquinoline quinone (PQQ) to treat premature ovarian insufficiency (POI) induced by cyclophosphamide (CTX) in rats restore ovarian function?

Design: Thirty female Sprague-Dawley rats were divided at random into three groups: control group (n = 10), POI group (n = 10), and POI-PQQ group (n = 10). An ovarian aging model was induced by CTX and treated with PQQ. The body weight of the rats was monitored throughout the process, and serum samples were collected to determine hormone concentrations.

Role of indoleamine 2,3-Dioxygenase 1 in modulating the malignant biological behavior of cervical cancer cells through the Tryptophan-Kynurenine pathway.

Objective: The aim of this study is to evaluate the influence of altered expression of indoleamine 2,3-dioxygenase 1 (IDO1) on key enzymes and metabolites within the tryptophan-kynurenine pathway, as well as to assess the resulting effects on the malignant biological behavior of cervical cancer cells, including proliferation, invasion and migration.

Methods: IDO1 expression was assessed in cervical cancer and normal cervical tissues via immunohistochemistry and western blot (WB) analysis. Four human cervical cancer cell lines (HeLa, C33A, SiHa, CaSki) were cultured, and HeLa and SiHa cells-characterized by elevated IDO1 expression-were selected for further experimentation.

Contextual interference effects on volleyball serve acquisition: a controlled trial with physical-education majors.

To address limitations in previous domestic and international studies on "the effect of background interference on volleyball learning", the present study selected 60 students majoring in physical education from the 2021 class at Qufu Normal University as experimental subjects to examine their skill acquisition. This study aimed to explore the differential effect of background interference on the learning of volleyball serving technique of students majoring in physical education. During an 8-week teaching experiment, the effects of three training modes, namely group block practice (low interference), mixed practice (medium interference), and random practice (high interference), on the learning effect of the serving technique of 60 physical education majors were compared.

A novel strategy of using ultraviolet a light emitting diodes (UVA-LED) irradiation extends the shelf-life and enhances the antioxidant property of minimally processed pakchoi ( subsp. ).

Ultraviolet A light-emitting diode (UVA-LED) is an emerging non-chemical technology with potential of enhancing postharvest quality of fresh produce. This study compared effects of UVA-LED irradiation (0-control, 4, 8, 12, 16, 20 J/cm) on senescence and shelf-life of minimally processed pakchoi (MPP). An 8 J/cm dose effectively preserved chlorophyll and color, extending 2 days shelf-life.

Changes of network controllability and structural-function coupling in cerebral small vessel disease.

This study aims to investigate potential changes in network controllability and structural-function coupling in cerebral small vessel disease (CSVD). Fifty-one CSVD patients and forty-one elderly controls underwent diffusion tensor imaging and resting-state functional magnetic resonance imaging. Average controllability and modal controllability were calculated using network control theory.

Feasibility and safety assessment of the AGIBOT robotic system in urological surgery: preliminary results from a multicenter study.

Background: Robotic-assisted surgery has become a standard approach for urological procedures, providing enhanced precision, dexterity, and minimally invasive benefits. However, high costs and limited accessibility limits its broader adoption. This study evaluates feasibility and safety of novel AGIBOT robotic system in complex urological surgeries.

Unveiling the mechanisms of CEBPD-orchestrated TAM polarization through RGS2/PAR2 pathway and its impact on ICB efficacy in clear cell renal cell carcinoma.

Background: The polarization status and function of tumor-associated macrophages (TAMs) influence tumor progression and patients' prognosis. CCAAT/enhancer-binding proteins (CEBPs) family are important transcriptional factors in macrophages differentiation physically and pathologically. This study aims to explore the mechanism of CEBPs in TAMs polarization in clear cell renal cell carcinoma (ccRCC) immune microenvironment and its impact on immune checkpoint blockers (ICBs) therapy.

Gene Mining and Optimization of the Biosynthesis of Cannabidiol in XG4-2.

Cannabidiol (CBD) exhibits various pharmacological properties, including antidepressant, antioxidant, antipsychotic, analgesic, and neuroprotective effects. However, the low natural yields of CBD and the restricted biosynthetic pathways currently limit its commercial viability and demand in the marketplace. This study evaluated the potential of 101 endophytic fungi from for biotransformation and CBD synthesis.

Prediction and reliability analysis of rigid pipeline response in soft soil using improved particle swarm neural network.

Pipelines in soft soil are prone to deformation and failure under traffic loads. Therefore, it is highly important to accurately characterize the dynamic response of pipelines under traffic loads and reasonably evaluate their operational status. First, for rigid pipelines, a Dload subroutine is written in the FORTRAN language to accurately characterize traffic loads, and a 3D numerical analysis model of the rigid pipe‒soil system is established using ABAQUS software to simulate the dynamic response of rigid pipelines in soft soil under traffic loads.

Locally advanced prostate cancer treated with neoadjuvant therapy combined with surgery: a multicenter retrospective cohort analysis.

Recent data from clinical trials have shown that neoadjuvant therapies significantly improve the pathological outcomes of prostate cancer patients. This study aimed to assess the specific pathological and prognostic effects of these therapies in a real-world, multicenter cohort. Additionally, we explored how factors such as the duration of neoadjuvant therapy and pretreatment imaging modality impact overall treatment outcomes within this therapeutic framework.

Evaluation of MRI-based brain oxygen extraction fraction mapping in patients with systemic lupus erythematosus.

Objective: We aim to assess the cerebral oxygen extraction fraction (OEF) changes in patients with systemic lupus erythematosus (SLE) and neuropsychiatric SLE (NPSLE) by using an MRI-based technique and examine the relationship between OEF and cognition.

Methods: 43 SLE patients (18 NPSLE and 25 non-NPSLE) and 26 healthy controls (HC) were recruited. Cognitive function was assessed via the Montreal Cognitive Assessment (MoCA).

Norcantharidin inhibits the METTL16/MAT2A pathway to induce apoptosis and suppress tumor progression in ovarian cancer.

Objective: This study aimed to investigate the antitumor effects of norcantharidin (NCTD) in ovarian cancer and to elucidate its molecular targets and mechanisms of action, with a focus on the methyltransferase-like protein 16 (METTL16)/methionine adenosyl transferase II alpha (MAT2A) signaling axis.

Materials And Methods: Expression levels of METTL16 and MAT2A were analyzed in ovarian cancer tissues and cells using immunohistochemistry, quantitative real-time polymerase chain reaction, and western blotting. Human ovarian cancer cell lines (ES2 and SKOV3) were cultured and subjected to METTL16 overexpression via gene recombination.

Adjustable Upcycling of Polyethylene Terephthalate to Biodegradable Polymer Monomers by Mn-Catalyzed Solvent Switching Strategy.

Waste plastics represent a new resource for the chemical industry. In this study, we demonstrate a solvent-switching strategy to upcycle waste polyethylene terephthalate (PET) into monomer terephthalic acid (TPA) and key bulk feedstocks for biodegradable polymers, such as lactic acid (LA) or glycolic acid (GA). PET undergoes rapid and mild depolymerization into its monomers TPA and ethylene glycol (EG) under the catalysis of a manganese complex.

Discovery of Coumarin-7-/-Acethydrazide Derivatives as Novel Antifungal and Herbicidal Agents.

Natural products (NPs) have attracted increasing attention due to their structural diversity, abundance, and low environmental impact, making them valuable for novel fungicide development. In this study, a series of coumarin-7-/ acethydrazide derivatives were synthesized by incorporating acetic acid moieties into the coumarin scaffold. The in vitro antifungal activities of these compounds were evaluated against six common plant pathogenic fungi.

High-sensitivity C-reactive protein as a mediator between sleep quality and mental health in first-degree relatives of individuals with schizophrenia.

Objective: This study investigates the mediating role of high-sensitivity C-reactive protein (hs-CRP) in the relationship between sleep quality and mental health in first-degree relatives of individuals with schizophrenia.

Methods: A total of 150 first-degree relatives of schizophrenia patients were recruited for this cross-sectional, observational study. Based on their Pittsburgh Sleep Quality Index (PSQI) scores, participants were divided into two groups: the Good Sleep Quality Group (GSQG, n = 77) and the Poor Sleep Quality Group (PSQG, n = 73).

GID4 recognition of Pro/N-degron peptides: Conformational selection and induced fit.

The N-degron pathway is essential for protein quality control and cellular homeostasis. GID4, a subunit of the GID ubiquitin ligase, is the main recognition component of the Pro/N-degron pathway. It binds protein substrates through their N-terminal proline, but its binding model and recognition of nonproline residues remain unclear.

Development and Evaluation of a Droplet Digital PCR Assay for the Accurately Detecting the CircHIPK3 in Plasma Samples from Patients with Hepatocellular Carcinoma.

Hepatocellular carcinoma (HCC) is an increasingly prevalent malignant neoplasm on a global scale. Circrna HIPK3 (circHIPK3) has been identified as playing a key role in HCC tumorigenesis and as a novel biomarker. In this study, we aimed at developing a sensitive and accurate method for the detection of circHIPK3 in low load plasma samples using a droplet digital PCR (ddPCR).