Engineering Aggregation-Induced Emission Photosensitizers through a Counterion-Modulation Strategy for Enhanced Photodynamic Immunotherapy.

The intersystem crossing (ISC) process of photosensitizers (PSs) is crucial for the generation of reactive oxygen species (ROS) in photodynamic immunotherapy. Herein, a counterion-regulation strategy is applied to enhance ISC efficiency in aggregation-induced emission (AIE) PSs, optimizing type-I ROS production. Three PSs with the same cationic donor-π-acceptor (D-π-A) structure, ,-diphenyl-4-(7-(pyridin-4-yl)benzo[][1,2,5]thiadiazol-4-yl)aniline (TBP), were synthesized with different counterions: iodide (I), hexafluorophosphate (PF), and tetraphenylborate (PhB).

Development of a predictive model for metachronous liver metastasis in gastric cancer.

Background: Patients with metachronous liver metastasis (MLM) in gastric cancer generally have a poor prognosis. Early detection and accurate prediction of MLM are crucial for improving clinical outcomes. This study aims to identify the risk factors for MLM through clinical pathological parameters and develop a predictive model for MLM in gastric cancer.

Microneedle-Delivered Multivalent MPXV DNA Vaccines Induce Promising Immunity Profiles and Cross-Protection in Mice.

Traditional DNA vaccines, typically administered via intramuscular injection with electroporation (IM-E), often cause discomfort and require trained personnel. Addressing these challenges, we developed multivalent DNA vaccines targeting both intracellular mature virion (IMV) and extracellular enveloped virion (EEV) proteins of the monkeypox virus (MPXV), designated as M2 (A29L, B6R), M3 (A29L, B6R, M1R) and M4 (A29L, B6R, M1R, A35R). These vaccine constructs were formulated into dissolvable microneedle array patches (D-MAPs) for intradermal delivery.

Interplay and integrated regulation of astaxanthin-lipid biosynthesis in microbial systems: A review from metabolic pathway cross-talk to biotechnological optimization.

Astaxanthin, a lipid-soluble carotenoid with antioxidant and anti-inflammatory properties, is mainly found in microorganisms. Its production is limited by lipid availability. Microbial lipids, like PUFAs, are also new food resources with health benefits.

Gut microbiota-derived butyric acid regulates calcific aortic valve disease pathogenesis by modulating GAPDH lactylation and butyrylation.

The involvement of gut microbiota in calcific aortic valve disease (CAVD) pathogenesis remains underexplored. Here, we provide evidence for a strong association between the gut microbiota and CAVD development. ApoE mice were stratified into easy- and difficult- to calcify groups using neural network and cluster analyses, and subsequent faecal transplantation and dirty cage sharing experiments demonstrated that the microbiota from difficult-to-calcify mice significantly ameliorated CAVD.

Optimal number of the examined lymph nodes for different N stages in colorectal cancer.

The National Comprehensive Cancer Network (NCCN) recommends that at least 12 lymph nodes (LNs) should be harvested during radical surgery of colorectal cancer (CRC). This study aims to clarify the optimal number of LNs that should be examined during the radical surgery for CRC patients with each N stage. The SEER database (n = 48,331) served as the primary cohort, while the Xijing cohort (n = 4447) was used for external validation.

BSA-Net: Boundary-prioritized spatial adaptive network for efficient left atrial segmentation.

Atrial fibrillation, a common cardiac arrhythmia with rapid and irregular atrial electrical activity, requires accurate left atrial segmentation for effective treatment planning. Recently, deep learning methods have gained encouraging success in left atrial segmentation. However, current methodologies critically depend on the assumption of consistently complete centered left atrium as input, which neglects the structural incompleteness and boundary discontinuities arising from random-crop operations during inference.

Comparison of flow diversion alone or combined with coiling for treatment of intracranial very large and giant aneurysms.

Objective: To compare the clinical and angiographic outcomes of flow diverter (FD) combined with coiling versus FD alone for intracranial very large (>2 cm) and giant aneurysms.

Methods: A retrospective analysis was conducted on 76 patients with intracranial very large/giant aneurysms treated at a single institution (April 2015-June 2024). Patients were divided into two groups: FD combined with coiling (FD + coiling group, n = 55) and FD alone (FD alone group, n = 21).

Mitochondrial inflexibility ignites tumor immunogenicity in postoperative glioblastoma.

Cellular and molecular heterogeneity contributes to the insufficient immunogenicity of glioblastoma multiforme (GBM), a lethal malignancy characterized by post-resection relapse, ultimately leading to limited immune cell infiltration. Here, we report a strategy to boost tumor immunity by activating the endogenous cGAS-STING signaling pathway through in-situ manipulation of the mitochondrial electron transport chain (ETC), thereby augmenting the immune responsiveness of GBM. Under white light irradiation, the synthetic butterfly-shaped photosensitizer B-TTPy disrupts the mitochondrial ETC by producing excessive reactive oxygen species.

Main results of a randomized controlled trial (RNPLS-01): efficacy and safety of ramucirumab combined with nab-paclitaxel, lobaplatin, and S-1 in neoadjuvant therapy for advanced gastric cancer.

Objective/background: The RNPLS-01 trial aimed to evaluate the efficacy and safety of ramucirumab (a VEGFR2 antagonist) combined with nab-paclitaxel, lobaplatin, and S-1 (RNPLS group) versus nab-paclitaxel, lobaplatin, and S-1 alone (NPLS group) in neoadjuvant therapy for advanced gastric cancer, as part of the RNPLS-01 prospective randomized controlled trial.

Methods And Analysis: RNPLS-01 trial enrolled 140 patients with advanced gastric cancer across two cohorts (neoadjuvant therapy: n = 70; conversion therapy: n = 70). This report focuses on the neoadjuvant cohort where patients were randomized 1:1 to receive either RNPLS (n = 35) or NPLS (n = 35).

AI-Driven segmentation and morphogeometric profiling of epicardial adipose tissue in type 2 diabetes.

Background: Epicardial adipose tissue (EAT) is associated with cardiometabolic risk in type 2 diabetes (T2D), but its spatial distribution and structural alterations remain understudied. We aim to develop a shape-aware, AI-based method for automated segmentation and morphogeometric analysis of EAT in T2D.

Methods: A total of 90 participants (45 with T2D and 45 age-, sex-matched controls) underwent cardiac 3D Dixon MRI, enrolled between 2014 and 2018 as part of the sub-study of the Swedish SCAPIS cohort.

One-Stage Full-Thickness Resection of Segmental Patterns Up-Lip Hemangiomas In Adults.

Background: Hemangiomas are usually managed by a "watch and wait" approach in infancy. However, many adults are still left untreated for a variety of reasons. This can often have significant effects on the patient's mental health and positive social interaction, especially when the hemangioma is located on the face.

Novel Highly Potent c-Met Degraders against a Broad Range of Cancers.

The cellular-mesenchymal epithelial transition factor (c-Met) is an attractive target in multiple cancers. Despite various c-Met inhibitors having been developed, the acquired drug resistance hampers their clinical application. In this study, through elaborately rational optimization, c-Met degraders, namely, , , and , were developed to exhibit single-digit nanomolar cell growth inhibition IC values, picomolar c-Met degradation DC values, and >99% of maximum degradation in cancer cells with alterations via a Cullin-CRBN-dependent pathway.

Genome structure mapping with high-resolution 3D genomics and deep learning.

Gene expression is often regulated by distal enhancers through cell-type-specific 3D looping interactions, but comprehensive mapping of these interactions across cell types is experimentally intractable. To address this gap, we introduce an integrated approach where we generate ultra-deep Region Capture Micro-C (RCMC) and Micro-C data specifically designed for state-of-the-art deep learning architectures. We developed Cleopatra, an attention-based deep learning model that takes epigenomic inputs and is pre-trained on genome-wide Micro-C data followed by fine-tuning with high-resolution RCMC data.

Targeting hepatocellular carcinoma growth and metabolism: A synergistic approach with para-toluenesulfonamide and radiofrequency ablation.

Background And Aims: Hepatocellular carcinoma (HCC) is one of the most prevalent and lethal forms of cancer, posing challenges to standard treatment methods. Radiofrequency ablation (RFA) has emerged as a valuable technique for the local control of advanced HCC, especially in cases unsuitable for surgical resection. Para-toluenesulfonamide (PTS) has demonstrated antitumor properties in various cancers, yet its efficacy against HCC remains underexplored.

Synthetic efferocytic receptor microglia enhances anti-inflammatory clearance of amyloid-β for AD treatment in mice.

Monoclonal antibody immunotherapy targeting the clearance of amyloid-β (Aβ) has shown promise in Alzheimer's disease (AD). However, current antibody treatments trigger Fc receptors and induce proinflammatory responses, in turn exacerbating neuronal damage. Here, we report a synthetic efferocytic receptor (SER) integrating Aβ-targeting scFv, efferocytosis receptor backbone based on TIM4 and downstream signal for microglia (MG) reprogramming, which enabled selective elimination of Aβ without inducing an inflammatory response.

Exploring the rarity: insights into primary diffuse large B-cell lymphoma of the prostate from a global retrospective analysis.

Background: The purpose of this study is to comprehensively analyze cases of primary diffuse large B-cell lymphoma of the prostate (P-DLBCL-P) from a global perspective, aiming to understand the disease's characteristics, treatment responses, and outcomes. By doing so, we seek to establish a valuable reference for the clinical management of this rare malignancy.

Materials And Methods: This study conducted a retrospective review of P-DLBCL-P cases reported worldwide, using various online databases, including PubMed, Scopus, and other English databases, as well as WanFang Data and China National Knowledge Infrastructure in Chinese, collecting clinical pathology information, treatment modalities, and prognosis of patients, and conducted survival analysis using the Kaplan-Meier method.

Changes in the complexation behavior of cadmium with dissolved organic matter in the Huaihe River basin: Environmental drivers, regional differences and socio-economic impacts.

The mobility of Cd (II) in aquatic ecosystems is significantly influenced by its complexation with dissolved organic matter (DOM), but the impact of multidimensional factors on this complexation process remains poorly understood. This study examined Cd (II) binding of DOM from four distinct regions-agricultural (AgriDOM), industrial (IndDOM), wetland (WetDOM), and estuarine (EstDOM)-in a pollution-entwined, agro-saturated river. Analysis was performed using fluorescence excitation-emission matrix spectroscopy (EEM), principal component coefficients, parallel factor analysis (PARAFAC), moving-window two-dimensional correlation spectroscopy (MW2DCOS), and structural equation modeling (SEM).

EDIL3 regulates pulmonary artery smooth muscle cell proliferation and migration via integrin αVβ3/ERK1/2 axis in pulmonary hypertension.

The abnormal expression of matricellular proteins involved in pulmonary vascular remodelling can accelerate the development of pulmonary hypertension (PH). Epidermal growth factor-like repeats and discoidin I-like domains 3 (EDIL3) is a matrix protein associated with the onset and progression of various diseases, yet its role in PH remains unexamined. This research aims to assess the expression of EDIL3 in PH and to investigate its underlying mechanisms in the condition.

Experimental and theoretical study on flexural performance of concrete beams strengthened by unbonded prestressed ultra-high performance concrete layer.

Ultra High Performance Concrete (UHPC) has the advantages of high strength and good durability. The new technology of unbonded prestressed UHPC to strengthen ordinary concrete beams can give full play to the performance of prestressed "high efficiency" and UHPC "light durability". In this paper, through the test and theoretical analysis of 10 unbonded prestressed UHPC reinforced beams, the bearing capacity of prestressed UHPC reinforced beams and the key parameters affecting the flexural performance (i.

Meditation as a non-pharmacological treatment for narcolepsy: A literature review.

Narcolepsy is a sleep disorder characterized by excessive daytime sleepiness (EDS), cataplexy, sleep paralysis, and hallucinations, and is often associated with other psychological and physiological disturbances. These symptoms lead to decreased social functioning and lower quality of life for individuals with narcolepsy. While current pharmacological treatment for narcolepsy can be effective in improving symptoms, there are also significant side effects (e.

Gastrodin mitigates aortic valve calcification by inhibiting glycolysis and histone lactylation through interfering with valve interstitial cells.

Calcific aortic valve disease (CAVD) is the most common disease of the heart valves and is characterised by thickening, fibrosis and calcification of the aortic valve leaflets. Gastrodin, the active component of the traditional Chinese medicine , has antioxidant, anti-inflammatory, anti-apoptotic and antiviral activities and is widely used in the treatment of neurological and cardiovascular diseases. Here, we report that gastrodin attenuates calcification in CAVD, but the underlying mechanism is unclear.

Impact of yeast extract on bacterial metabolism and nickel microbiologically influenced corrosion: Insights into medium optimization and biofilm electron transfer mechanism.

Yeast extract (YE) serves as a complex nutrient source and plays a pivotal role in the formation and development of microbial biofilms. This work elucidates the critical role of YE in modulating the metabolic activity of Desulfovibrio vulgaris, characteristics of passive films, and the associated microbiologically influenced corrosion (MIC) behavior of nickel (Ni). The presence of YE suppresses corrosion processes linked to extracellular electron transfer (EET) by reducing the necessity for D.

Gastrodin regulates H3K14la through the CDT2-KAT2A axis to treat Sepsis-induced myocardial dysfunction.

Sepsis-induced myocardial dysfunction (SIMD), a life-threatening complication of systemic infection, lacks effective therapies. This study investigated whether Gastrodin (GAS) alleviates SIMD by modulating ubiquitination and histone lactylation pathways. Using an in vivo lipopolysaccharide (LPS)-induced murine model and in vitro TNF-α-stimulated human cardiomyocytes (AC16), we evaluated the effects of GAS on myocardial injury, inflammation, and apoptosis.

Transcription factor binding divergence drives transcriptional and phenotypic variation in maize.

Regulatory elements are essential components of plant genomes that have shaped the domestication and improvement of modern crops. However, their identity, function and diversity remain poorly characterized, limiting our ability to harness their full power for agricultural advances using induced or natural variation. Here we mapped transcription factor (TF) binding for 200 TFs from 30 families in two distinct maize inbred lines historically used in maize breeding.