Macrophage Nogo-B drives liver fibrosis.

Background & Aims: Liver fibrosis is characterized by sustained injury stress, chronic inflammation, and repeated cell death and repair, all of which promote the progression of end-stage liver diseases (e.g., liver cirrhosis and carcinoma).

Plasma FSTL-1 as a noninvasive diagnostic biomarker for patients with advanced liver fibrosis.

Background And Aims: Reliable novel noninvasive biomarkers for the diagnosis of advanced liver fibrosis are urgently needed in clinical practice. We aimed to investigate the accuracy of plasma Follistatin-like protein 1 (FSTL-1) in the diagnosis of advanced liver fibrosis in chronic liver diseases.

Approach And Results: We collected cross-sectional clinical data for a derivation cohort (n = 86) and a validation cohort (n = 431), totaling 517 subjects with liver biopsy.

Tuning the rate performance in O2-type layered manganese-based oxides through cobalt doping.

Lithium-rich manganese-based cathode materials Li[LiNiMn]O have received considerable attention. However, severe voltage decay and structural distortion of O3-type layered oxides hinder further practical applications. O2-type layered cathode materials can restrict the movements of transition metals and effectively suppress the voltage decay.

Aspirin is associated with a reduction in mortality rate for patients with sepsis-induced coagulopathy: a retrospective cohort study.

Background: This study aimed to examine whether aspirin reduces mortality in patients with sepsis-induced coagulopathy (SIC).

Methods: In this retrospective cohort study, 1,194 patients with SIC were identified from the Medical Information Mart for Intensive Care (MIMIC)-IV database. The primary outcome was 28-day all-cause mortality.

Evaluation of live surgery continuing education course in implantology for dentists: a retrospective cross-sectional survey.

Background: Continuing education courses are vital for cultivating dental specialists. In traditional dental continuing education, training for implant surgery is typically delivered through various forms of demonstrations (such as videos, images) in courses, but this learning method lacks interactivity and detailed information on surgical procedures. However, live surgery can address these limitations.

METTL3 suppresses inflammatory responses by regulating SIRT1-mediated HMGB1 release in sepsis.

Sepsis, a life-threatening systemic inflammation caused by dysregulated host responses to infection, involves complex pathophysiological mechanisms. In eukaryotes, N6-methyladenosine (m6A), a prevalent post-transcriptional RNA modification, is dynamically regulated by "writers" (methyltransferases), "erasers" (demethylases), and "readers" (binding proteins). Although methyltransferase-like 3 (METTL3), a key writer, has been associated with multiple diseases, its role in sepsis remains unclear.

Development and validation of a machine learning-based model for predicting intraoperative blood loss during burn surgery.

Background: Intraoperative blood loss is a critical issue in the care of patients with burns. The timely identification of patients at elevated risk for substantial blood loss during surgical procedures is imperative.

Methods: Demographic data, laboratory test results, and surgical factors of patients were collected.

Immunogenicity evaluation of a recombinant pseudorabies virus co-expressing PCV2 and PCV3 capsid proteins in mice and piglets.

Porcine circovirus type 2 (PCV2), porcine circovirus type 3 (PCV3), and pseudorabies virus (PRV) are major pathogens posing significant threats to the swine industry. Viral evolution and mutations have limited the efficacy of current commercial vaccines, necessitating the development of more effective prophylactic strategies. In this study, a novel recombinant virus strain, designated as rPRV-ΔTK-PCV3(Cap)/ΔgIgE-PCV2(Cap), was generated using PRV SX-10 variant as the backbone.

Recombinant PRV Expressing GP3 and GP5 of PRRSV Provides Effective Protection Against Coinfection With PRV and PRRSV.

Porcine Reproductive and Respiratory Syndrome (PRRS) and Pseudorabies (PR) are highly contagious diseases caused by Porcine Reproductive and Respiratory Syndrome virus (PRRSV) and Pseudorabies virus (PRV). Due to the limited protective ability of currently commercialized vaccines against NADC30-like PRRSV and PRV variants, the pathological damage caused by coinfection of these two viruses has a significant impact on China's pig farming industry. In this study, six recombinant PRV stains with TK and gI/gE deletions and fused expression of GM-CSF and GP3 and GP5 proteins from NADC30-Like PRRSV were constructed by using the HDR-CRISPR/Cas9 system.

Interplay of Ferroptosis, Cuproptosis, Autophagy and Pyroptosis in Male Infertility: Molecular Crossroads and Therapeutic Opportunities.

Male infertility is intricately linked to dysregulated cell death pathways, including ferroptosis, cuproptosis, pyroptosis, and autophagy. Ferroptosis, driven by iron-dependent lipid peroxidation through the Fenton reaction and inactivation of the GPX4/Nrf2/SLC7A11 axis, disrupts spermatogenesis under conditions of oxidative stress, environmental toxin exposure, or metabolic disorders. Similarly, cuproptosis-characterized by mitochondrial dysfunction and disulfide stress due to copper overload-exacerbates germ cell apoptosis via FDX1 activation and NADPH depletion.

An approach for mapping phytoplankton communities in freshwater lakes based on phytoplankton absorption features.

Phytoplankton communities play a crucial role in the lake ecosystem due to their varying characteristics, functions, and impacts of different phytoplankton groups. Understanding the composition of phytoplankton groups in freshwater lakes is essential for comprehending geochemical processes and managing water quality. In this study, an improved Diagnostic Pigment Analysis method for freshwater lakes was developed and the proportion of five major phytoplankton groups-Dinophyta, Cryptophyta, Chlorophyta, Cyanophyta, and Bacillariophyta-was derived through the absorption-decomposition method.

Adrenomedullin Inhibits the Efficacy of Combined Immunotherapy and Targeted Therapy in Biliary Tract Cancer by Disrupting Endothelial Cell Functions.

The global incidence of biliary tract cancer (BTC) is on the rise, presenting a substantial healthcare challenge. The integration of immune checkpoint inhibitors (ICIs) with molecularly targeted therapies is emerging as a strategy to enhance immune responses. However, the efficacy and underlying mechanisms of these treatments in BTC are still largely unexplored.

Cognitive control in individuals with heroin use disorder after prolonged methadone maintenance treatment.

Background: Although impaired cognitive control is common during the acute detoxification phase of substance use disorders (SUD) and is considered a major cause of relapse, it remains unclear after prolonged methadone maintenance treatment (MMT). The aim of the present study was to elucidate cognitive control in individuals with heroin use disorder (HUD) after prolonged MMT and its association with previous relapse.

Methods: A total of 63 HUD subjects (41 subjects with previous relapse and 22 non-relapse subjects, mean MMT duration: 12.

Fructose-Bisphosphate Aldolase Mediating Pollen-Food Allergy Syndrome.

Pollen-food allergy syndrome is an IgE-mediated allergic reaction arising from cross-reactive homologous allergens found in both food and pollen. Allergens, such as pathogenesis-related protein class 10 and profilin, usually trigger oropharyngeal itching and numbness in patients, whereas lipid transfer proteins tend to induce anaphylaxis. This article presents a case study of an individual with Artemisia pollen allergy who experienced anaphylaxis after consuming red fruit ginseng, a perennial herb belonging to the Campanulaceae family.

Leveraging Patient-Derived Organoids for Personalized Liver Cancer Treatment.

Primary liver cancer (PLC) is a primary cause of cancer-related death worldwide, and novel treatments are needed due to the limited options available for treatment and tumor heterogeneity. 66 surgically removed PLC samples were cultured using the self-developed 2:2 method, and the final success rate for organoid culture was 40.9%.

Development and immunogenicity evaluation of a quadruple-gene-deleted pseudorabies virus strain.

Since 2011, the emergence of Pseudorabies virus (PRV) variants has led to significant vaccine failures, resulting in severe economic losses in China's swine industry. Conventional PRV vaccines have shown limited efficacy against these emergent variants, underscoring the urgent need for novel immunization strategies. This study aimed to develop and evaluate a novel recombinant PRV vaccine candidate with improved safety and immunogenicity profiles.

Multi-omics revealed the mechanism of feed efficiency in sheep by the combined action of the host and rumen microbiota.

This study was conducted to investigate potential regulatory mechanisms of feed efficiency (FE) in sheep by linking rumen microbiota with its host by the multi-omics analysis. One hundred and ninety-eight hybrid female sheep (initial body weight = 30.88 ± 4.

XBP1 Facilitating NF-κB-p65 Nuclear Translocation Promotes Macrophage-Originated Sterile Inflammation Via Regulating MT2 Transcription in the Ischemia/Reperfusion Liver.

Background & Aims: XBP1, most conserved transcription factor of endoplasmic reticulum stress, plays important roles in physiological and pathologic settings and has profound effects on disease progression and prognosis, so it is necessary to investigate XBP1 in macrophage-originated sterile inflammation during liver ischemia/reperfusion injury (IRI). Macrophage XBP1 expression and liver injury are analyzed in patients undergoing ischemia-related hepatectomy.

Methods: A myeloid-specific male XBP1-knockout (XBP1) strain is created for function and mechanism of XBP1 on macrophage-derived sterile inflammation in murine liver IRI with in vitro parallel research.

Comparative analysis of the complete mitogenomes of var. and var. provide insights into evolution and phylogeny relationship.

Introduction: Among cultivated tea plants (), only four mitogenomes for var. (CSA) have been reported so far but none for var. (CSS).

Transcriptional kinetics of NADC34-like porcine reproductive and respiratory syndrome virus during cellular infection.

NADC34-like porcine reproductive and respiratory syndrome virus (PRRSV) employs complex strategies to synthesize subgenomic RNAs (sgRNAs); however, their plasticity and temporal dynamics remain largely unexplored. Using next-generation sequencing (NGS), we examined the high-resolution landscape of the PRRSV subgenome, highlighting considerable heterogeneity in temporal kinetics and transcriptional control and revealing extensive coordination between TRS-dependent and TRS-independent sgRNAs. In addition, a comprehensive re-annotation of transcription regulatory sequence (TRS) locations was conducted, clarifying that their usage involved canonical, alternative, and non-canonical splicing events for annotated genes.

A novel CAF-cancer cell crosstalk-related gene prognostic index based on machine learning: prognostic significance and prediction of therapeutic response in head and neck squamous cell carcinoma.

Background: Cancer-associated fibroblast (CAF)-cancer cell crosstalk (CCCT) plays an important role in tumor microenvironment shaping and immunotherapy response. Current prognostic indexes are insufficient to accurately assess immunotherapy response in patients with head and neck squamous cell carcinoma (HNSCC). This study aimed to develop a CCCT-related gene prognostic index (CCRGPI) for assessing the prognosis and response to immune checkpoint inhibitor (ICI) therapy of HNSCC patients.

Oxymatrine and Gut Microbiota Modulation: A Potential Therapeutic Strategy for Bone Cancer Pain Management.

Chronic pain often coincides with changes in gut microbiota composition. Yet, the role of gut microbiota in bone cancer pain (BCP) is still not fully understood. This study investigated the role of gut microbiota in BCP and the effect of oxymatrine (OMT) on gut microbiota in BCP.