Global trends of antimicrobial resistance and virulence of Klebsiella pneumoniae from different host sources.

Background: Klebsiella pneumoniae is ubiquitous in animals, humans, and the environment, facilitating the dissemination of antimicrobial resistance (AMR) and virulence traits. Most studies are primarily focused on human clinical isolates, leaving critical gaps in understanding non-human reservoirs and cross-species transmission risks.

Methods: We combined large-scale genomic analyses with in vitro and in vivo infection models to characterize the evolutionary dynamics of 2809 K.

Synergistic Enhancement of Band Gap and Optical Anisotropy via Functional-Site Cosubstitution in Antiperovskite-Type Chalcohalides [BaK][X][MS] (M = Ge, Si; X = Cl, Br).

The advancement of infrared (IR) birefringent crystals has been persistently constrained by the mutually exclusive relationship between high Δ and wide bandgap () in traditional chalcogenides. Herein, we report the rational design and successful synthesis of antiperovskite-type chalcohalides [BaK][X][MS] (M = Ge, Si; X = Cl, Br) employing a functional-site cosubstitution strategy, where [Ba][S][GeS] was utilized as the prototype compound. Notably, the title compounds achieve a substantial improvement over the parent material [Ba][S][GeS], with Δ values increasing from 0.

Transcranial magnetic stimulation over right intraparietal sulcus improves selective attention for feature discriminating.

Background: Previous studies have confirmed the coordinated control of attentional processes by the frontal and parietal lobes. However, the function of the intraparietal sulcus (IPS) in the attention system remains unclear. In this study, we measured transcranial magnetic stimulation (TMS)-evoked event-related potentials (ERPs) to determine the extent to which the right intraparietal sulcus (rIPS) is involved in attentional processing.

Structure, Function and Application of Tremella Fuciformis Polysaccharide: A Review.

Tremella fuciformis (TF) is a valuable edible fungus rich in various nutrients, with TF polysaccharide (TFP) as its primary bioactive component. Extensive studies have demonstrated that TFP exhibits significant bioactivities, including antioxidant, antitumor, immunomodulatory, memory-enhancing, anti-inflammatory, hypoglycemic, and hypolipidemic effects, as well as skin-protective properties and gut microbiota modulation. Due to these multifunctional properties, TFP has garnered considerable attention in the food, pharmaceutical, and cosmetic industries.

Near diffraction-limited in-band pumped Ho:YLF composite thin disk laser at 2 μm.

A room-temperature composite thin disk laser is demonstrated based on a holmium-doped yttrium lithium fluoride (Ho:YLF) crystal. The composite thin disk structure was proposed and designed to enhance mechanical strength and suppress the ASE effect. The spectroscopic properties, including emission cross-section and fluorescence lifetime, of the 2 at.

Spatio-temporal distribution characteristics and sources of organic carbon in a typical mariculture Bay: A case study of Sansha Bay, China.

In the present study, a survey on the aquacultural environment and distribution characteristics of organic carbon were conducted in a typical mariculture bay of southeast China from January to August 2023. The spatio-temporal dynamics and sources of dissolved organic carbon (DOC) and particulate organic carbon (POC) were investigated, as well as the impact of mariculture on the coastal carbon cycles. The results showed that concentrations of DOC ranged from 0.

New trends and hotspots in sepsis-related protein post-translational modification: a bibliometric and visual analysis.

Background: Sepsis is a clinical syndrome characterized by high morbidity and mortality rates, posing a severe threat to human health. Its pathophysiology is complex, involving multiple physiological and pathological processes. Protein post-translational modification (PTM) play a pivotal role in the pathophysiology of sepsis by regulating inflammation, immune responses, and organ dysfunction.

The Influence of Parent-Youth Shared Language Erosion on Hispanic Immigrant Youth Anxiety Problems.

Objective: Shared language erosion (SLE) may contribute to anxiety in Hispanic immigrant youth by increasing stress and parent-youth conflict. This study examined the relationship between SLE and anxiety, considering parent-youth conflict and youth perceived stress as possible mediating factors.

Methods: Data were collected from 107 Hispanic immigrant 7th-grade youth (53% females) and their parents (88% mothers).

Electric field variations across DLPFC targeting methods in TMS therapy for Alzheimer's disease.

Background: The dorsolateral prefrontal cortex (DLPFC) is crucial for cognitive control and a primary target for transcranial magnetic stimulation (TMS) in Alzheimer's disease (AD). However, understanding the distribution of TMS-induced electric field (E-field) across different targeting methods remains limited, as does its relationship to therapeutic outcomes.

Objective: This study assesses differences in TMS-induced E-field using functional versus anatomical targeting methods for DLPFC stimulation.

MetaGeno: a chromosome-wise multi-task genomic framework for ischaemic stroke risk prediction.

Current genome-wide association studies provide valuable insights into the genetic basis of ischaemic stroke (IS) risk. However, polygenic risk scores, the most widely used method for genetic risk prediction, have notable limitations due to their linear nature and inability to capture complex, nonlinear interactions among genetic variants. While deep neural networks offer advantages in modeling these complex relationships, the multifactorial nature of IS and the influence of modifiable risk factors present additional challenges for genetic risk prediction.

Evaluation of the diagnostic efficacy of serum DR-70 and its combination with CEA in lung cancer.

Objective: To evaluate the diagnostic value of DR-70 immunoassay and its combination with carcinoembryonic antigen (CEA) measurement for detecting lung malignancies.

Methods: Serum concentrations of carcinoembryonic antigen (CEA) and DR-70 were quantitatively assessed using electrochemiluminescence immunoassay (ECLIA) and enzyme-linked immunosorbent assay (ELISA), in 110 patients diagnosed with primary lung cancer, 48 patients with pulmonary benign lesions, and 164 healthy subjects.

Results: Serum levels of both DR-70 and CEA showed statistically significant differences between lung cancer patients and healthy controls ( < 0.

A quadruplex real-time fluorescent quantitative PCR detection method for identification and serotyping of Pasteurella multocida.

Pasteurella multocida is a zoonotic pathogen with the ability to infect a diverse range of hosts, including humans through contact with pets. Due to its diverse capsular serogroups, rapid and accurate identification, as well as serotyping, are essential for the effective prevention and control of infections caused by this bacterium. This study developed a quadruplex quantitative PCR (qPCR) method for the identification and serotyping of P.

Isolation, Passage, and Pathogenicity of a Newly Isolated Strain From Hubei, China.

is the causative agent of porcine proliferative enteropathy (PPE)-a disease of great economic impact to the global pig industry, but the isolation and continuous passage culture of this bacterial species is very difficult which limits the development of inactivated or live vaccines. While China is the largest pig rearing country in the world, only one study has reported the isolation of . .

Activating and Rapid Self-Repairing Lattice Oxygen in High-Entropy Layered Hydroxides for Durable Oxygen Evolution.

The activation of lattice oxygen to participate in the oxygen evolution reaction (OER), thereby bypassing the theoretical limitations of the traditional adsorption evolution mechanism, represents a promising approach to enhancing the OER kinetics. However, there exists an inherent contradiction between the OER activity and stability of such catalysts due to the element overflow caused by the formation of oxygen vacancies during the OER. Herein, we present a compatibility strategy aimed at simultaneously enhancing activity and stability by incorporating catalytically inactive Zn into FeCoNiCu layered hydroxide (LDH).

[TeSeS]: The First Heterotriple-Chalcogenide Motifs Decode Giant Mid-Far Infrared Birefringence.

Optical anisotropy, a cornerstone of polarization control, presents fundamental challenge in functional optical material design due to the intricate interplay between its regulation mechanisms and performance optimization. The development of mid-far infrared (MFIR) birefringent crystals has long been shackled by the incompatibility between giant optical anisotropy and ultrabroad transparency in conventional chalcogenides. Here, we break this shackle via heterotriple-chalcogenide engineering in the first thiotellurite birefringent family, BaTeQ (Q = S, Se), featuring isolated [TeQ]⁻ motifs.

Light Intensity Alters Thermal Tolerance During Heat and Cold Stress in Canopy Plant Species.

Climate change increases extreme weather events like heat waves and cold waves. Understanding plant thermal tolerance is essential for assessing their safety. Measuring the Fv/Fm after dark thermal treatments is a common way to evaluate photosynthetic thermal tolerance, but it differs from real-world conditions with light-temperature covariation.

Hundred-watt-level cryogenic near diffraction-limited composite thin-disk Ho:YLF oscillator at 2 µm.

We present a cryogenic composite thin-disk oscillator based on a holmium-doped yttrium lithium fluoride (Ho:YLF) crystal that we believe is for the first time, with a hundred-watt-level near diffraction-limited laser output. Integrating the multi-pass pumping configuration and the cryogenic cooling technique, a maximum power of 128 W was achieved with an optical efficiency of 62.5% at 2 µm.

Genomic characteristics and pathogenicity of three newly isolated NADC34-like PRRSV strains in China and evaluation of one strain for inactivated vaccine candidate.

Porcine reproductive and respiratory syndrome (PRRS) has caused substantial economic losses to the pig industry. Recently, the emergence of NADC34-like PRRS virus (PRRSV) has made the worldwide circulation of PRRSV more complex. In 2017, the first detection of NADC34-like PRRSV in China was reported, but the pathogenicity and biological characteristics of this novel PRRSV variant remain to be further elucidated.

Picosecond laser ultrasonic imaging detection of near-surface micro defects using PCS and SAFT algorithm.

The effective detection and imaging of near-surface micro defects has been the key problem in the ultrasonic inspection field for a long time. Laser ultrasound has high resolution and sensitivity which is the effective technology to detect micro defects. In this paper, the time and frequency domain characteristics of the picosecond laser ultrasound and the interaction mechanism between micro defects and ultrasonic waves are studied by simulation and experiment.

The importance of cryptophytes in phytoplankton community in Xiangshan Bay.

Cryptophytes, as an important phytoplankton group, are usually neglected under traditional microscopical examination. We investigated the spatial and temporal variations of cryptophyte abundances in Xiangshan Bay (XSB) and their importance in the phytoplankton community using data from phytoplankton and environmental factors across four seasons in 2021. We found significant seasonal and spatial differences in cryptophyte abundances in XSB.

Individual electric field in cortical white matter is correlated with cognitive improvement in patients with mild cognitive impairment due to Alzheimer's disease after repetitive transcranial magnetic stimulation treatment.

BackgroundRepetitive transcranial magnetic stimulation (rTMS) may be effective for Alzheimer's disease (AD) and mild cognitive impairment (MCI); however, the therapeutic efficacy varies significantly, highlighting the need for reliable biomarkers to predict treatment response. While rTMS may activates cortical white matter, the relationship between induced electric field (E-field) in this region and clinical outcomes remains unclear.ObjectiveThis study characterized the E-field in cortical gray matter (EF), cortical white matter (EF), and region-of-interest (EF) in the left dorsolateral prefrontal cortex (DLPFC), and explored their correlations with treatment efficacy in patients with MCI due to AD.

Reduced glymphatic clearance in early psychosis.

Psychosis involves neuroinflammation and oxidative stress, both affecting the glymphatic system, the lymphatic-like, fluid-transport system in the brain. However, it is unclear whether early psychosis is related to impairments in glymphatic functions. In resting-state fMRI, it has been recently established in a number of neurodegenerative diseases that the coupling relationship between cortical blood-oxygen-level-dependent (BOLD) signal and ventricular cerebrospinal fluid (CSF) flow is associated with brain waste clearance, a key glymphatic function that has not been examined in psychosis or any other psychiatric populations.

A comprehensive exploration of the impact and contribution of polygenic risk score on age at onset of 30 complex diseases.

Objectives: Polygenic risk score (PRS) has increasingly shown promise in predicting disease risk; however, studies examining the influence of PRS on age at onset remain limited. This study aimed to systematically assess the impact of PRS on age at onset across multiple diseases.

Study Design: Prospective cohort study METHODS: We calculated PRS with two methods (C+T and PRS-CS) and compared their predictive capability in age at onset of 30 diseases in the UK Biobank.

Development and application of a triplex real-time PCR method for the detection of , , and .

(LI), (Bhy), and (Cp) are three common swine enteric pathogens widespread worldwide, leading to huge economic losses to the pig industry. LI, Bhy, and Cp are co-infected and should not be distinguished, and the detection of bacterial nucleic acids in feces allows rapid identification of whether a herd is infected with these three pathogens. In this study, we developed and optimized a TaqMan triple quantitative real-time PCR (qPCR) method for LI, Bhy, and Cp, which was validated and applied to the analysis of actual samples from pigs.