A Versatile DNAzyme-Amplified Protease-Sensing Platform for Accurate Diagnosis of SARS-CoV-2 and Reliable Classification of Colorectal Cancer.

Peptide-based biosensors are widely used for in vitro detection of protease activity but often suffer from the limited sensitivity, poor accuracy, and incompatibility with point-of-care testing (POCT) devices. Herein, we developed a versatile deoxyribozyme (DNAzyme)-amplified protease-sensing (DP) platform that integrates the positively charged oligopeptides with a negatively charged DNAzyme biocatalyst for highly-sensitive protease detection. The system leverages the electrostatic peptide-DNAzyme interactions to inhibit DNAzyme catalytic activity, which is reactivated upon the protease-triggered peptide hydrolysis, thus enabling an efficient signal amplification via the successive cleavage of DNAzyme substrate.

Surface engineering of cellulose microspheres via amyloid-like protein aggregation for bilirubin removal.

Hemoperfusion is one of the most effective blood purification techniques to quickly remove bilirubin from the blood of patients with kidney or liver failure. Although numerous adsorbent materials with high adsorption capacity have been developed, their clinical application are still limited due to poor biocompatibility and biosafety issues. Herein, biocompatible core-shell structured adsorbents with cellulose microspheres (CMs) as the supporting core and phase-transformed lysozyme (PTL) as the functional shell are fabricated for the removal of bilirubin in hemoperfusion.

ChREBP-Mediated Choline Deprivation and Chemokine Secretion Shape Tumor-Associated Macrophages to Promote Immune Evasion.

Tumor metabolic reprogramming has been recognized as a critical determinant in tumor development and cancer immunotherapy response. Aberrant choline metabolism is emerging as a defining hallmark of cancer. Here, we found that carbohydrate responsive element binding protein (ChREBP)-mediated choline deprivation induced tumor-associated macrophage (TAM) reprogramming and maintained an immunosuppressive tumor microenvironment (TME).

Crosstalk between mitochondrial dysfunction and benign prostatic hyperplasia: unraveling the intrinsic mechanisms.

Benign prostatic hyperplasia (BPH) represents a prevalent etiology of lower urinary tract symptoms (LUTS) in the male population, clinically defined by a non-malignant proliferation of prostatic tissue. While BPH exhibits a high prevalence among older male populations globally, the precise underlying mechanisms contributing to its development remain incompletely elucidated. Mitochondria, essential organelles within eukaryotic cells, are critical for cellular bioenergetics, the regulation of reactive oxygen species (ROS) generation, and the modulation of cell death pathways.

DBN1‑mediated upregulation of GAB2 facilitates the migration and invasion of T‑cell acute lymphoblastic leukemia cells.

T-cell acute lymphoblastic leukemia (T‑ALL) is an aggressive hematological malignancy. The poor prognosis of T‑ALL is closely associated with extensive leukemic infiltration into critical organs. Therefore, the mechanism underlying T‑ALL infiltration is worth investigating.

Hierarchical Structures of Protein-Fiber-Infused Aminoglycan Hydrogels to Promote Myocardial Repair.

Myocardial infarction (MI) is followed by irreversible damage to the myocardium, which eventually evolves into ventricular remodeling and heart failure. An imbalanced inflammatory response after MI can exacerbate myocardial injury. Current strategies to modulate inflammation and thereby improve myocardial tissue repair are limited.

Clinical features of high myopia with optic nerve head prelaminar schisis: Wuhan high myopia study.

Background: The global prevalence of high myopia is rising, posing a significant public health concern. Limited research exists on risk factors for prelaminar schisis (PLS) and its impact on visual field changes in highly myopic eyes. Herein, we investigated clinical features of prelaminar schisis (PLS) in highly myopic eyes.

Functional genetic screens reveal key pathways instructing the molecular phenotypes of tumor-associated macrophages.

Tumor-associated macrophages (TAMs) display remarkable functional heterogeneity, yet the molecular mechanisms driving their diverse phenotypes remain elusive. Using CRISPR screens in primary macrophages, we identified tumor-derived factors, including lactic acid, PGE2, and GM-CSF, as key modulators of TAM polarization. These factors interact synergistically and antagonistically to shape distinct TAM phenotypes that are highly conserved across human cancers.

ApoE4 Upregulates GSK-3β to Aggravate Alzheimer-Like Pathologies and Cognitive Impairment in Type 2 Diabetic Mice.

Background: The apolipoprotein E (ApoE) ε4 allele and type 2 diabetes mellitus (T2DM) are independent risk factors for Alzheimer's disease (AD), the most prevalent neurodegenerative disorder in the elderly. The T2DM patients carrying the ApoE ε4 allele exhibit heightened activation of platelet glycogen synthase kinase-3β (GSK-3β), a key downstream kinase in the insulin signaling pathway, along with more severe cognitive deficits. This observation suggests an intrinsic link between ApoE ε4, GSK-3β, and cognitive dysfunction.

Microglial Membrane-Coated Biomimetic Nanoplatform for Enhanced Blood-Brain Barrier Penetration and Targeted Photodynamic Therapy in Orthotopic Glioblastoma.

Glioblastoma multiforme (GBM) continues to pose a significant challenge in the field of neuro-oncology primarily because of the limited penetration of therapeutics across the blood-brain barrier (BBB) and the presence of immunosuppressive tumor microenvironments. To address these challenges, a HD-PEG@BM biomimetic nanoplatform (hereinafter referred to as HD-P@BM) is developed that cloaks the near-infrared II photosensitizer HD-PEG (HD-P) inside microglial membranes to enable enhanced BBB penetration and tumor-targeted delivery. In this study, it is found that the microglia-derived membranes enhanced the uptake of nanoparticles by both the glioma cells and tumor-associated microglia.

Mechanisms and Pathophysiological Significance of Insulin Resistance in Offspring With Intrauterine Growth Restriction Mediated by Hepatic GR/miR-1224 Programming.

Insulin resistance (IR) has fetal origin and gender preference. Here we traced glucose-insulin phenotypical changes of intrauterine growth restriction (IUGR) female offspring at different ages and explore the mechanism. The IUGR model was established by prenatal caffeine exposure (PCE): dams were orally administered caffeine [(30, 120 mg(kg.

Pathogenic glycosyltransferase genes and potential therapeutic drugs in pressure overload-induced heart failure.

Aims: Protein glycosylation regulated by glycosyltransferases is an important type of post-translational modification. The role of the glycosyltransferase genes (GTGs) in heart failure (HF) remains unclear and requires further investigation.

Methods: Differential expression analysis was performed on the transverse aortic constriction (TAC)-related dataset GSE36074 to screen out the differentially expressed GTGs.

Advances in therapeutic strategies for acute kidney injury.

Acute Kidney Injury (AKI) is a complex clinical syndrome marked by a rapid decline in renal function, most commonly triggered by ischemia, hypoxia, or nephrotoxic insults, and is associated with significant morbidity and mortality. Despite advances in understanding its underlying mechanisms, current treatments remain predominantly supportive, with no effective therapies available to reverse or repair renal damage. Although biomarker-guided precision medicine holds promise, its clinical utility requires further validation through large-scale trials.

Comparison of 68Ga-FAPI-04 and 18F-FDG in Primary Splenic Angiosarcoma.

Primary splenic angiosarcoma is a rare, aggressive tumor, characterized by rapid metastasis and poor prognosis. Herein, we report a case of 18F-FDG and 68Ga-FAPI-04 PET/CT imaging in a 57-year-old man with primary splenic angiosarcoma. 68Ga-FAPI-04 PET/CT presented much higher uptake in the lesions compared with 18F-FDG PET/CT, showing better diagnostic performance.

Research progress in glioma-related epilepsy (Review).

Epilepsy is a frequently occurring complication in patients with gliomas that substantially impairs their quality of life. The onset of epilepsy in patients with gliomas is driven by multiple mechanisms, including tumour-induced compression of the peripheral neural network, the release of neurotransmitters and inflammatory factors by tumour cells, alterations in the tumour microenvironment, and changes in gene expression. Collectively, these factors contribute to the abnormal excitability of local neurons, ultimately triggering seizures.

Deciphering cellular heterogeneity and pathway dynamics in urinary samples: a UMAP-Based approach to understanding acute kidney injury.

Background: Acute kidney injury (AKI) is characterized by rapid loss of renal function and is associated with severe clinical outcomes. Understanding the cellular heterogeneity in urine samples during AKI may provide insights into the underlying pathophysiological mechanisms and potential therapeutic targets.

Objectives: To explore the cellular composition and gene expression patterns in urine samples from AKI and non-AKI conditions using Uniform Manifold Approximation and Projection (UMAP) to identify key cellular interactions and pathway activations related to AKI.

Endogenous Enzyme Stimulation of Localized Catalytic DNA Assembly for High-Contrast microRNA Imaging and Reliable Evaluation of Drug Resistance.

Localized DNA circuits have emerged as a powerful platform for real-time visualization of disease-associated biomolecules in living cells, enabling deeper insights into complex biological processes. However, their extensive applications have been limited by a low probe utilization efficiency and nonspecific background signals. Here, we present a cell-specifically triggered localized catalytic assembly (TCA) circuit designed for high-contrast, reliable imaging of microRNAs (miRNAs).

Chinese Nurses' Perceptions of Intra-Organizational Workplace Psychological Violence: A Qualitative Study.

Intra-organizational workplace psychological violence has a significant impact on the physical and mental health of nurses. This study aimed to explore Chinese nurses' perceptions of intra-organizational workplace psychological violence and examine their attitudes, coping strategies, and the ramifications of such violent incidents. Semi-structured interviews were conducted with 18 nurses from a tertiary hospital in China, and the data were analyzed using the Colaizzi 7-step analysis method.

Integrin αvβ6-Targeted PET/CT Imaging of Non-Small Cell Lung Cancer with [Ga]Ga-Trivehexin: Improved Preoperative Lymph Node Staging and Association with Immunohistochemistry.

Integrin αvβ6, an epithelial-specific integrin, plays a crucial role in the development and prognosis of multiple tumors and has emerged as a potential target for cancer diagnosis and treatment. The novel integrin αvβ6-targeted radiotracer [Ga]Ga-Trivehexin shows excellent targeting specificity and favorable tumor-to-background contrast. In this prospective clinical study (NCT05835570) involving 58 participants with non-small cell lung cancer (NSCLC), [Ga]Ga-Trivehexin and 2-deoxy-2-[F]fluoro-D-glucose ([F]FDG) positron emission tomography/computed tomography (PET/CT) showed perfect diagnostic accuracy (100%, 58/58) for the detection of primary tumors.

Pelvic lipomatosis-A silent intruder in the pelvis: Clinical insights from a case report and literature analysis.

Rationale: This study aims to highlight the diagnostic challenges and multidisciplinary management of pelvic lipomatosis (PL), emphasizing imaging's pivotal role and the need for early intervention to mitigate long-term morbidity. With fewer than 200 reported cases, PL remains underrecognized; this case underscores its potential to mimic common gastrointestinal/urinary disorders, advocating for heightened clinical suspicion.

Patient Concerns: A 42-year-old male presented with a 2-day history of colicky abdominal pain under the xiphoid process, ac companied by nausea, vomiting, and watery stools.

Lipidomic profiling of human bile distinguishes cholangiocarcinoma from benign bile duct diseases with high specificity and sensitivity: a prospective descriptive study.

Background: Cholangiocarcinoma (CCA) is a rare and highly aggressive malignancy originating in the bile ducts. Owing to limitations involving pathological sampling, the clinical differentiation of CCA from benign biliary diseases remains challenging. This study aimed to evaluate the differences between the bile lipidomes of CCA patients and those of patients with benign disease to develop a bile lipid classifier that can help to differentiate CCA from benign conditions.

Multi-omics analysis of immunologically relevant genes in multiple sclerosis to identify potential drug target.

Background: Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system characterized by demyelination and immune dysregulation. Despite the established link with immune function, the genetic and pathological mechanisms connecting immune-related genes to MS remain incompletely understood.

Methods: We systematically integrated genome-wide association study (GWAS) data for various quantitative trait loci (QTLs) from public databases and literature, along with GWAS summary statistics for MS from IMSGC and FinnGen.