Assessing the Prognostic Accuracy of the PSS, pSOFA, and SIRS for In-Hospital Mortality in Children with Suspected Infection in Non-ICU Settings: A Multicenter Retrospective Study.

Objectives: To systematically compare the predictive accuracy of the Phoenix Sepsis Score (PSS), the Pediatric Sequential Organ Failure Assessment (pSOFA), and systemic inflammatory response syndrome (SIRS) in assessing in-hospital mortality risk among pediatric sepsis patients in non-ICU wards, thereby providing evidence-based support for clinical risk stratification.

Design: This study employed a multicenter retrospective cohort design, enrolling non-ICU pediatric patients with suspected infections (excluding preterm infants and neonates hospitalized immediately after birth), to construct an overall cohort and a neonatal subgroup cohort. Clinical parameters were collected through a data acquisition system, with parallel calculations of PSS, pSOFA, and SIRS scores.

Research progress in glioma-related epilepsy (Review).

Epilepsy is a frequently occurring complication in patients with gliomas that substantially impairs their quality of life. The onset of epilepsy in patients with gliomas is driven by multiple mechanisms, including tumour-induced compression of the peripheral neural network, the release of neurotransmitters and inflammatory factors by tumour cells, alterations in the tumour microenvironment, and changes in gene expression. Collectively, these factors contribute to the abnormal excitability of local neurons, ultimately triggering seizures.

Association between constipation and risk of stroke: a systematic review and meta-analysis.

Objective: This systematic review and meta-analysis aimed to evaluate the association between constipation and stroke risk, with subgroup analyses exploring effects on stroke subtypes.

Methods: We systematically searched PubMed, Web of Science, and Cochrane Library until February 2025. Published studies reporting adjusted odds ratios (ORs), hazard ratios (HRs), or relative risks (RRs) for stroke in constipated versus non-constipated individuals were included.

PGIP: a web server for the rapid taxonomic identification of parasite genomes.

Background: Parasitic diseases remain a global health challenge, and traditional methods in their diagnosis face limitations in sensitivity and scalability. Genome-based sequencing technologies have improved and are increasingly employed for the identification of parasites; however, their clinical adoption remains hindered by the complexity of bioinformatics analysis, reliance on incomplete reference databases, and accessibility barriers for nonspecialists. Overcoming these challenges necessitates the development of standardized analytical workflows and high-quality genomic resources specifically tailored for parasite identification.

Time-series analysis of climatic drivers of pediatric rotavirus and adenovirus infections in post-pandemic China.

Objective: To investigate the epidemiological characteristics and climate-sensitive transmission patterns of rotavirus (RV), adenovirus (AdV), and RV-AdV coinfections among children with acute gastroenteritis in Wuhan, China, during the post-COVID-19 era.

Methods: We conducted a retrospective time-series study of 53,088 pediatric patients tested for RV and AdV from April 2020 to August 2024. Age-stratified positivity rates were analyzed alongside temporal trends.

Case Report: endoscopic microvascular decompression for trigeminal neuralgia in a centenarian: a landmark case with 5-year follow-up.

Background: Endoscopic Microvascular Decompression (E-MVD) is a well-established effective treatment option for trigeminal neuralgia (TN). However, its application in ultra-elderly patients, particularly centenarians, has never been documented.

Case Description: A centenarian with TN for five years presented to our hospital in January 2020.

Novel MAP1B loss-of-function variant associated with periventricular nodular heterotopia 9 and literature review on genotype-phenotype associations of MAP1B.

Background: Periventricular nodular heterotopia 9 (PVNH9) arises from defective neuronal migration, which leads to the accumulation of ectopic neurons near the lateral ventricle. PVNH9 is caused by a heterozygous mutation in the microtubule-associated protein 1B (MAP1B) gene.

Methods: We report a PVNH9 family with a MAP1B variant.

Facile expression of proteins with desired N-terminal amino acid via an engineered cysteine protease domain.

The removal of N-terminal methionine (Met) or fused N-terminal purification tags to expose the first amino acid of the target protein is critically important for studying the potential regulatory role of the N-terminal residue. However, current tag-removal approaches typically rely on enzymatic cleavage or require harsh reaction conditions. Here, we report a strategy for expressing proteins of interest (POI) with custom N-terminal amino acids by introducing an engineered cysteine protease domain (CPD) tag at the N-terminus.

Analyzing in Wuhan: Serotypes, Antimicrobial Resistance, and Public Health Implications.

Objective: This study aims to delineate the epidemiological characteristics and antibiotic resistance of isolates from Wuhan, focusing on serotype distribution, resistance patterns, and genetic diversity.

Methods: Our study analyzed 40 isolates collected from 2011 to 2022 in Wuhan, assessing their serotype distribution and resistance to multiple antibiotics. We conducted resistance gene detection and genetic diversity analysis using polymerase chain reaction and pulsed-field gel electrophoresis (PFGE), respectively.

DNA box-assisted T7 transcription techniques combined with Cas13a for detection of the influenza A (H1N1) virus.

The influenza A (H1N1) virus continues to undergo mutations, posing a serious threat to public health. In this study, an innovative system was developed using a transcriptional isothermal amplification scheme combined with a DNA box for detection of H1N1 RNA. The split T7 promoter was assembled on four edges of the hexahedral DNA box to form two target-capturing robotic arms.

Functional Characterization of in Regulating Leaf Morphogenesis and Photosynthesis in .

Leaves are essential for photosynthesis and transpiration, directly influencing plant growth and development. Leaf morphology, such as length, width, and area, affects photosynthetic efficiency and transpiration rates. In this study, we investigated the role of in leaf morphogenesis by generating both overexpression and CRISPR/Cas9 knockout lines in .

Molecular determinants of HERG potassium channel blockade by domiphen bromide and benzethonium chloride.

Domiphen bromide (DMP) and benzethonium chloride (BZT) are synthetic quaternary ammonium compounds widely used as disinfectants. Both agents are potent inhibitors of the human ether-à-go-go-related gene (HERG) potassium channel, a key contributor to cardiac repolarization. Dysfunction of HERG is associated with long QT syndrome and arrhythmias, yet the molecular mechanisms underlying DMP and BZT inhibition remain incompletely understood.

Synergistic Adsorption and Triboelectrocatalysis in Multilayer Fabrics for Efficient 2-Nonenal Elimination.

2-Nonenal, a primary component of the characteristic "aging odor," poses minimal toxicity but significantly affects quality of life and psychological well-being, necessitating its effective removal. However, the existing removal methods mainly rely on adsorption of activated carbon or odor masking after diffusion, which suffers from low efficiency and environmental concerns. This study presents an approach for eliminating 2-nonenal through a multilayered composite textile system that integrates the synergistic effects of amine-based adsorption and triboelectric-driven mechanisms, transforming waste static electricity into valuable resources.

The Characterization of a Chitosan Coating Enriched with Bamboo-Leaf Flavonoids and Its Effect on Postharvest Preservation of Three Horticultural Products.

Chitosan coatings have been demonstrated to be a highly effective and safe approach to extending the shelf life of food. This study, for the first time, evaluates the effectiveness of bamboo-leaf flavonoids (BLFs) added to a chitosan coating to delay the spoilage of strawberries, blueberries, and bamboo shoots. The addition of BLFs improved the tensile strength of the coatings.

Annexin-A1 deficiency uncovers female-specific pathways in blood pressure control and cardiovascular remodeling in mice.

Cardiovascular disease exhibits distinct sex-based differences, yet the mechanisms underlying these differences remain under-explored. The pro-resolving mediator annexin-A1 (ANXA1) is a pivotal regulator in inflammation resolution and tissue homeostasis, including within the cardiovascular system. However, the sex-specific differences in ANXA1 in blood pressure regulation have not been investigated.

Alkoxy Side Chain Engineering in Metal-Free Covalent Organic Frameworks for Efficient Oxygen Reduction.

Metal-free covalent organic frameworks (COFs) gain significant attention as catalysts for the oxygen reduction reaction (ORR), a key process in energy conversion technologies like fuel cells and metal-air batteries. While substantial efforts are devoted to unravelling the mechanisms, by which heteroatom-containing building blocks in linkers, vertices, and linkages, enhance catalytic activity and selectivity, the potential of side-chain engineering to modulate pore wall surfaces and optimize the catalytic environment remains largely underexplored. This study investigates the role of alkoxy side chains in modulating the properties of COFs to enhance ORR performance.

Leveraging gut microbiota for enhanced immune checkpoint blockade in solid tumor therapy.

Gut microbiota can modulate antitumor immunity and influence immune checkpoint blockade (ICB) therapy efficacy and treatment-associated toxicity. Variations in the therapeutic effect of ICB among individuals are partially attributed to microbiota. This review summarizes current knowledge on how specific bacterial species enhance or hinder ICB outcomes by regulating immune cell activation, antigen presentation, and systemic inflammation.

L-Ergothioneine promotes the formation of long gold nanowires and their 2D assembly.

Gold nanowires with large aspect ratios, renowned for their exceptional mechanical properties, geometry-dependent conductance, and distinct plasmon peaks, are promising for applications in plasmonics, sensors, nanoelectronics, catalysis, and wearable optoelectronics. Traditional synthesis methods, such as lithography and template-assisted electrodeposition, often involve complex procedures or require seeding steps, making them less suitable for scalable integration into wearable optoelectronic devices. Here, we report a seedless and aqueous synthesis of gold nanowires (15-40 nanometers in diameter and 10-20 micrometers in length) at room temperature, utilizing natural amino acid L-ergothioneine (EGT) and quaternary alkylammonium halides (CTAX) as structure-directing agents and ascorbic acid (AA) as a redox agent.

Macrophage polarization in disease therapy: insights from astragaloside IV and cycloastragenol.

Dysregulated activation and polarization of macrophages drive the pathogenesis of diverse diseases, including inflammatory, autoimmune, ischemic, metabolic disorders, and cancers. Despite therapeutic advances, precise regulation of macrophage polarization remains challenging. Natural products have recently emerged as promising therapeutic regulators.

Accuracy of the diagnosis of gastroesophageal reflux disease by a trial of potassium-competitive acid blocker treatment.

Aim: The aim of this study was to explore the accuracy of the diagnosis of gastro-esophageal reflux disease (GERD) through tegoprazan treatment trials, and to analyze factors that may influence test accuracy.

Methods: This was a single-blind, single-arm 2 weeks tegoprazan treatment trials from March 2023 to April 2024. Patients with 'typical' reflux or heartburn as their most troublesome symptom who were considered likely to have GERD were recruited.

Correction: Feng et al. Mannose Receptor-Mediated Carbon Nanotubes as an Antigen Delivery System to Enhance Immune Response Both In Vitro and In Vivo. 2022, , 4239.

In the original publication [...