Mechanisms and Pathophysiological Significance of Insulin Resistance in Offspring With Intrauterine Growth Restriction Mediated by Hepatic GR/miR-1224 Programming.

Insulin resistance (IR) has fetal origin and gender preference. Here we traced glucose-insulin phenotypical changes of intrauterine growth restriction (IUGR) female offspring at different ages and explore the mechanism. The IUGR model was established by prenatal caffeine exposure (PCE): dams were orally administered caffeine [(30, 120 mg(kg.

Generation of a PHF19 knockout human embryonic stem cell line by CRISPR/Cas9 system.

PHD finger protein 19 (PHF19) is a polycomb protein that promoted cardiac hypertrophy via epigenetic targeting SIRT2. To determine the role of PHF19 in myocardial hypertrophy, we established a large fragment knockout model of PHF19 gene in human embryonic stem cells (hESCs-H7) using the CRISPR/Cas9 system based on a vector. This PHF19-KO cell line has a normal karyotype, classical human pluripotent stem cell morphology, strong pluripotency, and significantly reduced PHF19 gene expression, which will become a useful tool for further in-depth research on the pathogenesis of PHF19 gene deficiency induced myocardial hypertrophy.

Pelargonidin-3-O-glucoside prevents isoproterenol-induced myocardial infarction via modulating cardiac and inflammatory markers expression in experimental rats.

IntroductionThis study examined the cardioprotective effects of Pelargonidin-3-O-glucoside (Pg3G) against myocardial infarction induced by isoproterenol (ISO) in male Wistar rats.MethodsAnimals were divided into four groups each groups contain six animals. Group 1 control; Group 2 Pg3G treated control; Group 3 ISO-control; Group 4 Pg3G + ISO treated rats.

An Aberrant Resurgence of Endogenous Retroviruses Prompts Myocarditis and Heart Failure.

Background: Endogenous retroviruses (ERVs) occupy >8% of the human genome. Aberrant resurgence of ERVs has been implicated recently in several critical pathologies. However, the possible incidence and role of ERV resurgence in heart failure (HF), a leading cause of global morbidity and mortality, remain unexplored.

Prognostic and therapeutic relevance of IL2RG-related LncRNAs in clear cell renal cell carcinoma.

Interleukin-2 Receptor Subunit Gamma (IL2RG) has been implicated in various cancers, but its role in clear cell renal cell carcinoma (ccRCC) remains unclear. This study aimed to explore IL2RG expression, its relationship with IL2RG -related lncRNAs (IRLs). Gene expression and clinical data for ccRCC were obtained from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus(GEO) database.

Mosaic tetrasomy 9p detected by CNV-seq but missed by traditional karyotyping in a prenatal case without dysmorphic features.

Introduction: A unique case of mosaic tetrasomy 9p was found using CNV-seq analysis of uncultured amniocytes, which was missed by karyotype analysis of cultured amniocytes.

Case Report: The karyotype was determined to be 46, XY, with mosaic duplication of chromosome 9 from p24.3 to p13.

Perinatal factors for antepartum hemorrhage in women with placenta accreta spectrum.

Introduction: Placenta accreta spectrum with antepartum hemorrhage is closely related to maternal and fetal morbidity and mortality. It is of utmost importance to predict the possibility of antepartum hemorrhage using perinatal factors before delivery in women with placenta accreta spectrum. The aim of this study is to identify the risk factors for antepartum hemorrhage in women with placenta accreta spectrum.

The relationship between ethical leadership, moral sensitivity, and moral courage among head nurses.

Background: Moral courage is a key factor in accelerating the implementation of moral behavior. Effective ethical leadership can enhance head nurses' moral competence-particularly moral sensitivity, which serves as a cornerstone of the moral decision-making process. However, there is limited evidence on how ethical leadership influences moral courage.

Comprehensive analysis of plasma cell heterogeneity and immune interactions in multiple myeloma.

This study focused on the role of plasma cells in multiple myeloma (MM) and the associated potential mechanisms. Transcriptomic data of MM and various gene sets from several public databases were downloaded for subsequent analyses. Through single-cell sequencing, 10 major cell types were identified and annotated.

Low power energy balanced clustering routing scheme based on improved SSA and Multi-Hop transmission in IoT.

In the large scale monitoring application of the IoT, the power carried by a single sensor node is limited, and it may not be replenished in the later stage. Therefore, it is required that the sensor nodes should save power when working, and the power consumption among the sensor nodes should be balanced to avoid the early death of some nodes and the occurrence of monitoring blind spots in the monitor area. Communication is the main part of power loss for sensor nodes.

Residential greenness exposure and repeatedly measured hyperglycemic markers in women with gestational diabetes mellitus: A birth cohort study in Foshan, China.

Objectives: To investigate the association between residential greenness exposure and repeatedly measured hemoglobin A1c (HbA1c) levels during pregnancy in women with gestational diabetes mellitus (GDM).

Study Design: This was a cohort study.

Methods: This study was conducted in Foshan City, Southern China from January 1, 2016 to August 31, 2022.

Transcriptomic profiling of blood platelets identifies a diagnostic signature for pancreatic cancer.

Background: Pancreatic cancer (PaCa) is a deadly malignancy that is often diagnosed at an advanced stage, limiting treatment and reducing survival. There is an urgent need for convenient and accurate diagnostic markers for the early detection of PaCa.

Methods: In this multicenter case-control study, we performed transcriptome analysis of 673 platelet samples from different in-house and public cohorts.

Human-induced pluripotent stem cell-derived exosomes promote skin wound healing through activating FGF2-mediated p38 pathway.

The acute and large area skin healing has been an intractable problem for both clinician and patient. Exosomes derived from human-induced pluripotent stem cells (hiPSC-Exos) have been a novel promising cell-free treatment on skin damage repair. In this study, in vivo skin trauma model of full-layer skin damage on mouse back and in vitro skin-like trauma model of human keratinocytes (HaCaT) scratches were established to investigate the effects of hiPSC-Exos on the acute wound healing, and its potential regulation mechanism would be tried to explore.

Calcium-sensing receptors promoted Homer1 expression and osteogenic differentiation in bone marrow mesenchymal stem cells.

Homer1 interacts with calcium-sensing receptors (CaSRs) in osteoblasts (OBs), with both CaSR and Homer1 playing essential roles in AKT phosphorylation. This study investigated the impact of CaSR on Homer1 expression during the differentiation of rat bone marrow mesenchymal stem cells (BMSCs) at morphological, imaging, and molecular levels, both and . A post-oophorectomy osteoporosis model was established in Sprague-Dawley rats, validated through micro-computed tomography, hematoxylin-eosin staining, and biomechanical testing to assess changes in CaSR expression.

Hesperidin alleviates endothelial cell inflammation and apoptosis of Kawasaki disease through inhibiting the TLR4/IĸBα/NF-ĸB pathway.

Kawasaki Disease (KD) is an acute and self-limiting vasculitis of unknown etiology that mainly occurs in infancy and can lead to vascular endothelial injury. Hesperidin (HES) is an economical dietary biological flavonoid with anti-oxidant, anti-inflammatory, and anti-apoptotic pharmacological effects. The main objective of this study was to investigate the protective effects of HES on KD, and try to elucidate the underlying mechanism.

A missense variant in the SOX5 gene (c.221C > T) is associated with intellectual disability.

Objectives: The SOX5 gene has been identified as the pathogenic gene responsible for Lamb-Shaffer syndrome. In this study, we examined the SOX5 variant (c.221C > T, p.

Prediction nomogram for antepartum hemorrhage in placenta previa women.

Background: Placenta previa with antepartum hemorrhage (APH) is common and closely related to maternal and fetal morbidity and mortality. It is of the utmost importance to prepare for the possibility of APH using perinatal factors prior to delivery.

Objective: To develop and validate a nomogram predicting APH in women with placenta previa based on the perinatal factors.

The protective roles of eugenol on type 1 diabetes mellitus through NRF2-mediated oxidative stress pathway.

Type 1 diabetes mellitus (T1DM), known as insulin-dependent diabetes mellitus, is characterized by persistent hyperglycemia resulting from damage to the pancreatic β cells and an absolute deficiency of insulin, leading to multi-organ involvement and a poor prognosis. The progression of T1DM is significantly influenced by oxidative stress and apoptosis. The natural compound eugenol (EUG) possesses anti-inflammatory, anti-oxidant, and anti-apoptotic properties.

PTN secreted by cardiac fibroblasts promotes myocardial fibrosis and inflammation of pressure overload-induced hypertrophic cardiomyopathy through the PTN-SDC4 pathway.

Aims: Hypertrophic cardiomyopathy (HCM) is characterized by unexplained left ventricular hypertrophy (LVH) with key pathologic processes including myocardial necrosis, fibrosis, inflammation, and hypertrophy, which are involved in heart failure (HF), stroke, and even sudden death. Our aim was to explore the communication network among various cells in the heart of transverse aortic constriction (TAC) surgery induced HCM mice.

Materials And Methods: Single-cell RNA-seq data of GSE137167 was downloaded from the Gene Expression Omnibus (GEO) database.

Glycyrrhizin Attenuates White Matter Injury by Inhibiting Neuroinflammation through the HMGB1/TLR4 Pathway.

White matter injury (WMI) is a common complication of preterm birth, potentially resulting in long-term behavioral and motor abnormalities. The objective of this study is to investigate the neuroprotective effects of glycyrrhizin (GLY) on WMI, and try to elucidate the potential mechanisms. In vivo chronic hypoxia-induced WMI mouse model and in vitro oxygen-glucose deprivation (OGD) induced WMI cell model were established, and the effects of GLY on WMI were explored through multiple assays, such as western blotting, immunofluorescence, immunohistochemistry, behavioral experiments, real-time quantitative polymerase chain reaction (RT-qPCR), transmission electron microscope (TEM), molecular docking, and bioinformatics analysis.

Case Report: Novel compound heterozygous variants cause achromatopsia in three patients from a family.

This study report a novel missense variant in the gene identified by targeted gene panel sequencing approach in a Chinese family with achromatopsia. The proband, a 24-year-old female, with normal intelligence, motor development and speech abilities exhibited nystagmus, amblyopia, photophobia, and indistinguishable colors. In addition, the two sisters of the proband had the same clinical symptoms, which means that three patients from a family with a monochromasia clinical diagnosis.

Gut Microbiota Dysbiosis Facilitates Susceptibility to Bloodstream Infection.

To study the role of intestinal flora in the development of bloodstream infections (BSIs). 42 patients and 19 healthy controls (HCs) were screened into the study and their intestinal flora was measured by 16S rRNA gene sequencing. The bacterial diversity was significantly lower in the BSI group compared with that in the HCs (P < 0.

Exploring the dynamics of gut microbiota, antibiotic resistance, and chemotherapy impact in acute leukemia patients: A comprehensive metagenomic analysis.

Leukemia poses significant challenges to its treatment, and understanding its complex pathogenesis is crucial. This study used metagenomic sequencing to investigate the interplay between chemotherapy, gut microbiota, and antibiotic resistance in patients with acute leukemia (AL). Pre- and post-chemotherapy stool samples from patients revealed alterations in microbial richness, taxa, and antibiotic resistance genes (ARGs).