DBN1‑mediated upregulation of GAB2 facilitates the migration and invasion of T‑cell acute lymphoblastic leukemia cells.

T-cell acute lymphoblastic leukemia (T‑ALL) is an aggressive hematological malignancy. The poor prognosis of T‑ALL is closely associated with extensive leukemic infiltration into critical organs. Therefore, the mechanism underlying T‑ALL infiltration is worth investigating.

Graphene Oxide-Functionalized Optical Sensor for Label-Free Detection of Breast Cancer Cells.

Accurate and noninvasive detection of cancer cells is critical for advancing early stage cancer diagnostics and monitoring tumor progression. While manual enumeration methods, such as hemocytometry, remain in use, they suffer from limited sensitivity and scalability. In this article, we report the first feasibility study demonstrating a graphene oxide (GO)-functionalized long-period fiber grating (LPG) sensor for the label-free detection of MCF-7 human breast cancer cell density via secreted cellular byproducts.

Mechanistic Study on Orpiment Pigment Discoloration Induced by Reactive Oxygen Species.

Orpiment (AsS), a yellow mineral pigment widely used in historical artworks, undergoes degradation that seriously threatens the integrity of Dunhuang murals. Reactive oxygen species (ROS) exist widely in air, which may be one reason for the color change of pigments. This study aims to investigate the degradation effects and mechanisms of four ROS-hydroxyl radical (·OH), singlet oxygen (O), peroxynitrite anion (ONOO), and hydrogen peroxide (HO)-on orpiment.

Evolution of water-soluble organic aerosol composition from clean days to haze episodes in rural area of North China Plain.

Water-soluble organic aerosol (WSOA) plays a significant role in air quality and human health. Here we characterized the chemical properties and molecular compositions of WSOA at a rural site in North China Plain during winter using a high-resolution aerosol mass spectrometer and electrospray ionization high-resolution orbitrap mass spectrometer (ESI-HRMS). Our results show that the mass concentration of WSOA was significantly higher than that observed in Beijing in winter 2020, contributing about 56 % of OA on average.

ARID1A loss plus CD8+ T cell infiltration associate with favorable clinical outcomes in urothelial carcinoma.

Purpose: ARID1A, encoding a component of the SWI/SNF complex, is frequently mutated in urothelial carcinoma (UC). However, its specific impacts on clinical outcomes and CD8+ T cell functions in UC remain poorly understood.

Experimental Design: The clinical relevance of ARID1A loss and CD8+ T cell infiltration was evaluated in three cohorts (ZSHS, n = 135, FUSCC, n = 118, IMvigor210, n = 274).

NSPLformer: exploration of non-stationary progressively learning model for time series prediction.

Although Transformers perform well in time series prediction, they struggle when dealing with real-world data where the joint distribution changes over time. Previous studies have focused on reducing the non-stationarity of sequences through smoothing, but this approach strips the sequences of their inherent non-stationarity, which may lack predictive guidance for sudden events in the real world. To address the contradiction between sequence predictability and model capability, this paper proposes an efficient model design for multivariate non-stationary time series based on Transformers.

WGCNA and single-cell analysis reveal ferroptosis-related gene signatures for hepatocellular carcinoma prognosis and therapy.

Background: Hepatocellular carcinoma (HCC) is a globally serious malignant tumor with high incidence and mortality. Ferroptosis, a newly discovered form of regulated cell death, is significant in tumor initiation and growth. Herein, we performed bioinformatics analysis in order to investigate the expression heterogeneity of ferroptosis-related genes as well as its correlation with HCC clinical outcomes.

Notch activation downregulates EZH2 to thereby attenuate endothelial cell proliferation and angiogenesis via MYC destabilization.

Aims: Angiogenesis, a tightly regulated process involving dynamic endothelial cell (EC) proliferation, is critical in both physiological and pathological contexts such as ocular neovascular disorders. While Notch signaling is known to regulate angiogenesis, its downstream molecular mechanisms remain incompletely understood.

Main Methods: Gene-modified mice with a Cdh5-Cre transgene were generated to block Notch signaling in ECs.

Fatigue as a central bridge: temporal dynamics between problematic smartphone use and depressive symptoms in Chinese adolescents.

Background: The prevalence of problematic smartphone use (PSU) has been increasing among adolescents in recent years, often co-occurring with depressive symptoms, which poses additional challenges to adolescent mental health. Despite growing concern, the mechanisms underlying the co-occurrence of PSU and depression remain poorly understood. To address this gap, the current study employed cross-lagged panel network analysis to investigate the temporal relationships between specific symptoms of PSU and depressive symptoms over time.

PM concentration 7-day prediction in the Beijing-Tianjin-Hebei region using a novel stacking framework.

High-precision prediction of near-surface PM concentration is a significant theoretical prerequisite for effective monitoring and prevention of air pollution, and also provides guiding suggestions for the prevention and control of PM-related health risks. It has been acknowledged that existing PM prediction models predominantly rely on variables influenced by near-surface factors. This inherent limitation could hinder the comprehensive exploration of the continuous spatio-temporal characteristics associated with PM.

Optimized derivation and culture system of human naïve pluripotent stem cells with enhanced DNA methylation status and genomic stability.

Human naïve pluripotent stem cells (PSCs) hold great promise for embryonic development studies. Existing induction and culture strategies for these cells, heavily dependent on MEK inhibitors, lead to widespread DNA hypomethylation, aberrant imprinting loss, and genomic instability during extended culture. Here, employing high-content analysis alongside a bifluorescence reporter system indicative of human naïve pluripotency, we screened over 1,600 chemicals and identified 7 promising candidates.

CeO-based catalysts for photocatalytic degradation of volatile organic compounds: A comprehensive review.

Volatile organic compounds (VOCs) are hazardous air pollutants that pose significant risks to human health, highlighting the need for efficient and sustainable removal technologies. While titanium dioxide (TiO) has long been the dominant photocatalyst for VOCs degradation, cerium dioxide (CeO) has emerged as a promising alternative due to its unique Ce/Ce redox behavior and abundant oxygen vacancies. However, its photocatalytic efficiency is often limited by poor light absorption and rapid charge recombination.

Aging and Discoloration of Red Lead (PbO) Caused by Reactive Oxygen Species Under Alkaline Conditions.

Red lead (PbO) has been extensively utilized as a red pigment for centuries. However, the discoloration and blackening of red lead in historical paintings have significantly compromised the aesthetic value of mural artworks. Investigating the mechanisms behind the blackening of PbO is of paramount importance.

Roles of Brd4 in Vascular Smooth Muscle Cells: Implications for Aging and Vascular Dysfunction.

Background: Growing evidence suggests that the epigenetic reader Brd4 (bromodomain-containing protein 4) is involved in aging and aging-related diseases. However, the specific mechanisms by which Brd4 influences vascular aging, especially senescence of vascular smooth muscle cells (SMCs), remain unexplored.

Methods: Primary cell cultures were established using mouse aortic SMCs and treated with Brd4 inhibitor, ARV-825, or (+)-JQ1.

Perovskite-Graphene Heterostructure Biosensor Integrated with Biotunable Nanoplasmonic Ternary Logic Gate for Ultrasensitive Cytokine Detection.

The integration of 2D-materials and optoelectronic devices has attracted great attention for advanced applications. We propose the first perovskite/graphene heterostructure-based FET biosensor with uniquely biotunable ternary logic gating functionality. The biosensor integrates a lateral perovskite-on-graphene heterostructure phototransistor with a vertical bio-nano-photonic filter, with a decoupled construction inset.

Inhibition of DLK1 regulates AT2 differentiation and alleviates established pulmonary fibrosis by upregulating TTF-1/CLDN6.

Background: Idiopathic pulmonary fibrosis (IPF) is a devastating age-related disease with unknown causes and limited effective treatment. Dysregulation of Alveolar Type 2 (AT2) cells facilitates the initiation of IPF. While differentiation of AT2 into AT1 is necessary for restoring alveolar epithelium.

Hydrothermal valorization of waste EnteromorphaProlifera: Assist in the degradation of tetracycline and multifunctional product synthesis.

Enteromorpha Prolifera (EP), a non-native macroalgae, has significantly damaged coastal agricultural ecosystems through its rapid expansion. Thus, developing an eco-friendly and cost-effective waste conversion strategy is urgently required. To address this issue, this study presents a hydrothermal-assisted multifunctional product preparation (HMP) strategy, aimed at enhancing the value-added processing of EP.

Targeting endothelial MYC using siRNA or miR-218 nanoparticles sensitizes chemo- and immuno-therapies by recapitulating the Notch activation-induced tumor vessel normalization.

The chaotic, over-activated tumor vasculature promotes tumor growth and erodes most current therapies. Although Notch activation critically regulates angiogenesis, the broad roles of Notch has dampened its druggability. Gene-modified mice with a Cdh5-Cre transgene were employed to activate/block Notch signaling in endothelial cells (ECs).

Characterizing the impact of podophyllotoxin on pulmonary toxicity and gut-lung microbiota interactions in SD rats based on TEC concept.

Unlabelled: Podophyllotoxin (PPT), an extract from the traditional medicinal plant , offers anti-viral and anti-cancer benefits, though its use is limited by toxicity. The mechanisms of PPT's inherent pulmonary toxicity remain elusive. This study leverages the novel "Toxicological Evidence Chain" theory to explore the potential involvement of the "gut-lung axis" in PPT-induced pulmonary toxicity.

Exploration of key pathogenic mechanisms and potential intervention targets of the traditional Chinese medicine Coptis chinensis in the treatment of cervical cancer based on network pharmacology and molecular docking techniques.

Background: Traditional Chinese medicine (TCM) has shown potential in the treatment of cancer. This study investigated the molecular targets and mechanisms of Coptis chinensis in the treatment of cervical cancer using network pharmacology and bioinformatics.

Methods: Effective Coptis chinensis components were screened from the Traditional Chinese Medicine Systems Pharmacology (TCMSP) platform based on the following criteria: drug-like properties (DLP) ≥0.

Association of escitalopram-induced shifts in gut microbiota and sphingolipid metabolism with depression-like behavior in wistar-kyoto rats.

The microbiota-gut-brain axis plays a pivotal role in neuropsychiatric disorders, particularly in depression. Escitalopram (ESC) is a first-line antidepressant, however, its regulatory mechanisms on the microbiota-gut-brain axis in the treatment of depression remain unclear. The antidepressant effects of ESC were evaluated using the forced swim test in Wistar-Kyoto (WKY) rats, while damage in the gut and brain regions was assessed through H&E staining and immunohistochemistry.

Lethal clinical outcome and chemotherapy and immunotherapy resistance in patients with urothelial carcinoma with MDM2 amplification or overexpression.

Background: The E3 ubiquitin ligase murine double minute 2 (MDM2) binds the p53 transcriptional activation domain and acts as a potent inhibitor of pathway, one of the three most crucial oncogenic pathways in urothelial carcinoma (UC). However, the clinical significance and impact on tumor immune contexture of amplification in UC remain unclear.

Methods: This study analyzed 240 patients with UC with matched clinical annotations from two local cohorts (ZSHS cohort and FUSCC cohort).

Zeolite-like algal biochar nanoparticles for enhanced antibiotics removal: Sorption mechanisms and theoretical calculations.

In the study, Sargassum horneri (S. horneri) was used to create a novel zeolite-like algal biochar (KSBC). KSBC with doping of N, O, S, Al, and Si, displayed zeolite-like properties, including well-developed porosity, a high specific surface area (1137.

Capsule endoscopy aspiration and respiratory physician's treatment insights: a case report and literature review.

Background: Capsule endoscopy (CE) is widely used for intestinal examination; however, capsule aspiration into the airway is a serious complication that requires urgent intervention. We present a management case report and review 39 cases from 2003 to 2023, providing insights into the prevention and treatment of capsule aspiration.

Case Presentation: A 69-year-old man with chronic bronchitis and emphysema presented with 7 months of intermittent melena.

Pulmonary coccidioidomycosis in China: Case reports and literature review.

Coccidioidomycosis is a fungal infection commonly found in the tropical regions of southwestern United States, such as Arizona, the Central Valley of California, parts of New Mexico, and western Texas. The endemic regions also extend into northern Mexico and include focal endemic areas in sections of Central America and Argentina. Coccidioides species have also been reported in central and southern Utah, Nevada, and the central part of Washington State.