Single layer centrifugation combined with brief abstinence for semen cryopreservation in a patient with hematospermia: Case report and literature review.

Rationale: Efficient semen preservation is important in patients with hematospermia (HS). Few reports have delineated effective methods for the removal of red blood cells (RBCs), and existing techniques have exhibited constraints that affect sperm preservation. There is a paucity of studies on semen preservation in HS patients.

PM concentration 7-day prediction in the Beijing-Tianjin-Hebei region using a novel stacking framework.

High-precision prediction of near-surface PM concentration is a significant theoretical prerequisite for effective monitoring and prevention of air pollution, and also provides guiding suggestions for the prevention and control of PM-related health risks. It has been acknowledged that existing PM prediction models predominantly rely on variables influenced by near-surface factors. This inherent limitation could hinder the comprehensive exploration of the continuous spatio-temporal characteristics associated with PM.

Seminal hyperviscosity is associated with poor sperm quality and function.

Purpose: Male infertility affects many couples worldwide. Seminal hyperviscosity (SHV) is increasingly recognized as a contributor to male infertility by impairing sperm movement. However, the precise mechanisms by which SHV affects sperm parameters and functions remain unclear.

The association between greenspace exposure and sperm quality: A retrospective cohort study focusing on sperm kinematics and the influence of buffer settings.

Background: While previous studies have suggested a potential link between greenspace and semen quality, the effect of greenspace exposure on sperm kinematics, as well as the impact of greenspace estimation scales, remains underexplored.

Objectives: This study aimed to examine the association between greenspace exposure and sperm kinematics, and to determine whether this relationship varies across different greenspace estimation scales.

Methods: We conducted a retrospective longitudinal study of 5025 semen measurements from 1674 sperm donors recruited by the Sichuan Provincial Human Sperm Bank (SHSB) between June 2019 and December 2021.

Structural Simplification of Luotonin F: Discovery of Quinoline Derivatives as Novel Antifungal Agents for Plant Protection.

Inspired by the natural quinoline alkaloid Luotonin F, a series of quinoline-3-hydrazide derivatives were designed and synthesized by structure simplification and evaluated for their fungicidal activities against plant phytopathogenic fungi. The bioassay results showed that these compounds exhibited broad-spectrum and highly efficient antifungal activities . Among them, compound exhibited broad-spectrum inhibitory activities against , , , , , and with EC values of 0.

Arsenic Exposure Triggers Nonalcoholic Fatty Liver Disease through Repressing -Adenosylmethionine-Dependent Histone Methylation in Rats.

Arsenic (As) is a toxic metalloid widespread in the environment, and its exposure has been associated with a variety of adverse health outcomes. As exposure is demonstrated to cause nonalcoholic fatty liver disease (NAFLD), and the underlying epigenetic mechanisms remain largely unknown. This study aimed to investigate the roles of histone modifications in low-level As exposure-induced NAFLD in rats.

How has the external quality assessment/proficiency testing of semen analysis been developed in the past 34 years: a review.

Male infertility has become an important issue of global concern. Semen analysis is the cornerstone of male fertility assessment. External quality assessment (EQA) of sperm concentration, motility, and morphology is widely recognized in the world.

Sperm RNA code in spermatogenesis and male infertility.

Spermatozoa are traditionally thought to be transcriptionally inert, but recent studies have revealed the presence of sperm RNA, some of which is derived from the residues of spermatocyte transcription and some from epididymosomes. Paternal sperm RNA can be affected by external factors and further modified at the post-transcriptional level, for example N-methyladenosine (mA), thus shaping spermatogenesis and reproductive outcome. This review briefly introduces the origin of sperm RNA and, on this basis, summarizes the current knowledge on RNA modifications and their functional role in spermatogenesis and male infertility.

Critical windows for exposure to chemical composition of ambient particulate matter and human semen quality decline.

Background: Critical windows for exposure to chemical components of particulate matter (PM <2.5 μm in diameter [PM]) associated with the human semen quality decline remain unclear.

Objectives: To address this gap, we developed a new analytical framework by integrating a Linear Mixed Model (LMM) with subject- and center-specific intercepts and a Distributed Lag Model (DLM) to fully account for correlations between finely vulnerable exposure windows based on complete profile of the spermatogenesis cycle.

Design, Synthesis, and Biological Evaluation of Novel Quinoline Derivatives against Phytopathogenic Bacteria Inspired from Natural Quinine Alkaloids.

A series of 2-(trifluoromethyl)-4-hydroxyquinoline derivatives were designed and synthesized with introduction of the antibacterial fragment amino alcohols, and their antibacterial activity against plant phytopathogenic bacteria was evaluated for the development of quinoline bactericides. It is worth noting that compound exhibited excellent antibacterial activity with a minimum inhibitory concentration (MIC) value of 3.12 μg/mL against ().

Delayed covering causes the accumulation of motile sperm, leading to overestimation of sperm concentration and motility with a Makler counting chamber.

According to the World Health Organization (WHO) manual, sperm concentration should be measured using an improved Neubauer hemocytometer, while sperm motility should be measured by manual assessment. However, in China, thousands of laboratories do not use the improved Neubauer hemocytometer or method; instead, the Makler counting chamber is one of the most widely used chambers. To study sources of error that could impact the measurement of the apparent concentration and motility of sperm using the Makler counting chamber and to verify its accuracy for clinical application, 67 semen samples from patients attending the Department of Andrology, West China Second University Hospital, Sichuan University (Chengdu, China) between 13 September 2023 and 27 September 2023, were included.

Design, synthesis and antimicrobial activity of novel berberine derivatives.

Background: The threats to the safety of humans and the environment and the resistance of agricultural chemicals to plant pathogenic fungi and bacteria highlight an urgent need to find safe and efficient alternatives to chemical fungicides and bactericides. In this study, a series of Berberine (BBR) derivatives were designed, synthesized and evaluated for in vitro and in vivo antimicrobial activity against plant pathogenic fungi and bacteria.

Results: Bioassay results indicated that compounds A11, A14, A20, A21, A22, A25, A26, E1, E2, E3, Z1 and Z2 showed high inhibitory activity against Sclerotinia sclerotiorum and Botrytis cinerea.

Customizing carrier screening in the Chinese population: Insights from a 334-gene panel.

Objective: This study aimed to evaluate the yield and applicability of expanded carrier screening and propose carrier rate screening thresholds suitable for the Chinese population by comparing the current screening panel with the American College of Medical Genetics and Genomics recommended panel of 113 genes.

Methods: Using targeted next-generation sequencing, a customized panel with 334 genes was performed on 2168 individuals without clinical phenotypes for expanded carrier screening purpose. Variant interpretation followed the American College of Medical Genetics and Genomics guidelines.

Preparation and Screening of SRB Gel Particles Used for Deep Purification of Acid Mine Drainage.

The progressive decline of the coal industry necessitates the development of effective treatment solutions for acid mine drainage (AMD), which is characterized by high acidity and elevated concentrations of heavy metals. This study proposes an innovative approach leveraging sulfate-reducing bacteria (SRB) acclimated to contaminated anaerobic environments. The research focused on elucidating the physiological characteristics and optimal growth conditions of SRB, particularly in relation to the pH level and temperature.

Comprehensive molecular analyses and experimental validation of CDCAs with potential implications in kidney renal papillary cell carcinoma prognosis.

Previous reports have revealed that the abnormal expression of the cell division cycle-associated gene family (CDCAs) is closely associated with some human cancers. However, the precise functional roles and mechanisms of CDCAs in kidney renal papillary cell carcinoma (KIRP) remain unclear. In this study, RNA sequencing data from the Cancer Genome Atlas database and Genotype-Tissue Expression databases were utilized to perform the expression, correlation, survival, mutation, functional enrichment analysis, and immunoinfiltration analyses of CDCAs in KIRP.

Altered N6-methyladenosine methylation level in spermatozoa messenger RNA of the male partners is related to unexplained recurrent pregnancy loss.

Background: Understanding the pathogenesis of unexplained recurrent pregnancy loss is paramount for advancing effective treatments. Various biological processes, including spermatogenesis and embryo development, are tightly regulated by N6-methyladenosine modifications. However, few studies have focused on the impact of sperm N6-methyladenosine modifications on embryonic development.

Beneficial effect of residential greenness on sperm quality and the role of air pollution: A multicenter population-based study.

Background: Poor sperm quality is a major cause of male infertility. However, evidence remains scarce on how greenness affects male sperm quality.

Objectives: To assess the associations of residential greenness with male sperm quality and the modification effect of air pollution exposure on the relationship.

A review of electrooxidation systems treatment of poly-fluoroalkyl substances (PFAS): electrooxidation degradation mechanisms and electrode materials.

The prevalence of polyfluoroalkyls and perfluoroalkyls (PFAS) represents a significant challenge, and various treatment techniques have been employed with considerable success to eliminate PFAS from water, with the ultimate goal of ensuring safe disposal of wastewater. This paper first describes the most promising electrochemical oxidation (EO) technology and then analyses its basic principles. In addition, this paper reviews and discusses the current state of research and development in the field of electrode materials and electrochemical reactors.

Fertility preservation in male adolescents with cancer (2011-2020): A retrospective study in China.

Background: According to the studies, more than 80% of pediatric patients with cancer can achieve a survival rate greater than 5 years; however, long-term chemotherapy and/or radiation therapy may seriously affect their reproductive ability. Fertility preservation in adolescents with cancer in China was initiated late, and related research is lacking. Analyze data to understand the current situation and implement measures to improve current practices.

Homozygous variant in DRC3 (LRRC48) gene causes asthenozoospermia and male infertility.

Human infertility affects 10-15% of couples. Asthenozoospermia accounts for 18% of men with infertility and is a common male infertility phenotype. The nexin-dynein regulatory complex (N-DRC) is a large protein complex in the sperm flagellum that connects adjacent doublets of microtubules.

[Frequency and characteristics of Y chromosome microdeletions and karyotypic abnormalities among 4 278 infertile male patients from southwest China].

Objective: To determine the frequency and characteristics of AZF microdeletions of Y chromosome and karyotypic abnormalities among infertile male patients from southwest China.

Methods: 4 278 infertile male patients treated at West China Second University Hospital of Sichuan University from September 2018 to July 2023 were selected as the study subjects. Results of Y chromosome microdeletion detection and G-banded karyotyping analysis were retrospectively reviewed.

Fertility preservation in males with cancer of trends, region development, and efficacy in mainland China from 16 regions Chinese sperm banks.

Purpose: Male cancer survivors experience confusion about fertility following cancer treatment. The aims of this study were to evaluate survivors' semen quality in different tumor type groups in China and to analyze the current situation and challenges of male cancer patients with sperm cryopreservation.

Methods: This was a multicenter retrospective study of male patients with cancer who underwent sperm cryopreservation in 16 regions of the national sperm banks over an 11-year period from 2010 to 2020.