Prevalence and spectrum of STRC variants in 1015 sensorineural hearing loss patients: insights from the Chinese population.

Mutations in the STRC gene are a major cause of autosomal recessive mild-to-moderate sensorineural hearing loss (SNHL); however, its prevalence and mutational spectrum in the Chinese population remain largely unexplored. To address this, we analyzed 1015 unrelated Chinese patients with bilateral SNHL using whole-exome sequencing (WES), multiplex ligation-dependent probe amplification (MLPA), and Sanger sequencing. Biallelic STRC variants were identified in 2.

AMA1 regulates host cell apoptosis through the mitochondrial pathway and the death receptor pathway.

can cause severe damage to the organism by regulating host cell apoptosis during parasitic infection. apical membrane antigen 1 (AMA1) is integral to the invasion process and intracellular survival of . To investigate whether AMA1 affects host cell apoptosis during parasite infection, we first constructed an AMA1 expression system.

Single layer centrifugation combined with brief abstinence for semen cryopreservation in a patient with hematospermia: Case report and literature review.

Rationale: Efficient semen preservation is important in patients with hematospermia (HS). Few reports have delineated effective methods for the removal of red blood cells (RBCs), and existing techniques have exhibited constraints that affect sperm preservation. There is a paucity of studies on semen preservation in HS patients.

Deliver CEBPE via cartilage targeting Lipid nanoparticle to block CEBPE-LTF-STAT3 positive feedback loop for efficient treatment of cartilage endplate degeneration.

Intervertebral disc degeneration (IVDD) is recognized as a significant underlying factor contributing to clinical neck and low back pain. The cartilaginous endplates (CEP) serve as a selectively permeable barrier, which is essential for maintaining the internal homeostasis of the intervertebral disc (IVD). Dysfunction of the CEP is closely related to the initiation and progression of IVDD.

Activation of Sirt1 protects from single-walled carbon nanotubes-induced pulmonary fibrosis by inhibiting alveolar macrophage senescence.

Purpose: Expanding industrial applications of carbon nanotube (CNT) have led to the concern for the increased risk of pulmonary fibrosis. The current study investigated the role of alveolar macrophages (AMs) senescence during single-walled CNT (SWCNT)-provoked pulmonary fibrosis and the underlying mechanism.

Methods: Mice were intratracheally instilled with 40 μg SWCNT, then sacrificed on days 1, 7 and 28 post administration.

Seminal hyperviscosity is associated with poor sperm quality and function.

Purpose: Male infertility affects many couples worldwide. Seminal hyperviscosity (SHV) is increasingly recognized as a contributor to male infertility by impairing sperm movement. However, the precise mechanisms by which SHV affects sperm parameters and functions remain unclear.

Novel cancer stem cell-targeted retinoid ZSH-512 impedes colorectal cancer progress via KDM3A-mediated epigenetic reprogramming.

Advanced colorectal cancer (CRC) exhibits weak responses to multiple therapies, primarily due to the presence of cancer stem cells (CSCs), which drive high recurrence rates, metastasis, and drug resistance. We have previously systematically conducted CSC-targeted compound discovery and evaluation studies to inhibit CSC-mediated tumorigenesis and metastasis. Here, we identified ZSH-512, a novel synthetic retinoid that selectively targets retinoic acid receptor (RAR)γ, demonstrating its ability to effectively inhibit CRC-CSCs and patient-derived organoids (PDOs) and significantly reduce CSC-mediated tumor formation and liver metastasis in mouse models without noticeable toxicity.

One size fits null: attentional brain responses differ depending on insomnia subtype.

Study Objectives: Event-related potential (ERP) studies on attentional brain processes in insomnia disorder (ID) have yielded inconsistent findings. Such inconsistencies may relate to small sample sizes, limited corrections for multiple comparisons, and the possibility of heterogeneity within the clinical population. We aimed to overcome these limitations by studying ERP responses both across and within subtypes in a larger sample of ID.

Predicting preeclampsia in early pregnancy using clinical and laboratory data via machine learning model.

Background: This study was performed to characterize the relationship of various laboratory test indicators with clinical information and Preeclampsia (PE) development. Then, prediction models for early-onset preeclampsia (EOPE), late-onset preeclampsia (LOPE), and preterm preeclampsia (Preterm PE) were developed using maternal characteristics and laboratory data.

Methods: Between January 2019 and December 2021, we retrospectively recruited 144 EOPE, 363 LOPE, 231 Preterm PE, and 1458 healthy participants from six hospitals.

A stress-tolerant strain Rhodococcus sp. WH103 was isolated and co-immobilized to more efficiently degrade phenazine-1-carboxylic acid.

Phenazine-1-carboxylic acid (PCA), the main active ingredient of the bio-fungicide shenqinmycin, has been widely used in agriculture due to its excellent antimicrobial properties. However, it poses risks to non-target microorganisms and causes phytotoxicity, necessitating efficient degradation strategies. In this study, six PCA-degrading bacterial strains were isolated from the rice rhizosphere by enrichment culture.

How effective is acupuncture in treating hot flashes in breast cancer patients? A systematic review and meta-analysis.

Background: Although acupuncture is recommended for managing breast cancer-related hot flashes, the level of evidence is limited. With the updating of randomized controlled trials, it is necessary to reassess its efficacy.

Objective: To assess the effectiveness of acupuncture in the treatment of hot flashes in patients with breast cancer.

Long non-coding RNA AC133552.2: biomarker and therapeutic target in osteosarcoma via PANoptosis gene screening.

Osteosarcoma, the most common primary bone cancer in children and adolescents, presents significant challenges, particularly in metastasis and recurrence, resulting in poor survival rates. This study explores the role of PANoptosis-a complex cell death mechanism involving pyroptosis, apoptosis, and necroptosis-in osteosarcoma by identifying relevant long non-coding RNAs (lncRNAs) and their prognostic significance. Bioinformatics analyses used RNA expression data from the GEO and TARGET databases to identify differentially expressed genes (DEGs) and PANoptosis-related genes (PRGs).

Development and evaluation of protective immunity of a ROP27 DNA vaccine against Eimeria tenella in chickens.

This study aimed to develop and evaluate the protective immunity of a ROP27 DNA vaccine against Eimeria tenella (E. tenella) in chickens. E.

Spinosin enhances non-rapid eye movement sleep and alters c-Fos expression in sleep-wake regulatory brain regions in mice.

Purpose: Spinosin, a key flavonoids component found in Semen Zizhiphi spinosae, is known to enhance pentobarbital-induced sleep, which is primarily assessed with the loss-of-righting reflex (LORR). This research focused on investigating the impact of spinosin on sleep regulation in typical murine models.

Methods: We used electroencephalogram (EEG) and electromyogram (EMG) recordings to evaluate the effects of spinosin (10, 20, 40 mg/kg, i.

Observation of anomalously large Nernst effects in conducting polymers.

As a fundamental thermoelectric phenomenon in many solid-state materials, the Nernst effect has yet to be observed in conducting polymers. This knowledge could provide important insight into their elusive mechanism, which are crucial for flexible optoelectronic and thermoelectric applications. However, within the Landau's Fermi-liquid picture, the Nernst coefficient has demonstrated to be proportional to the charge mobility, and thus should be negligible in less ordered polymers with inherent low mobility.

ZSH-2208: A novel retinoid with potent anti-tumour effects on ESCC stem cells via RARγ-TNFAIP3 axis.

Backgroud: Oesophageal cancer ranks among the most prevalent malignant tumours globally, primarily consisting of oesophageal squamous cell carcinoma (ESCC). Cancer stem cells (CSCs) accelerate the progression ESCC via their strong self-renewal and tumourigenic capabilities, presenting significant clinical challenges due to increased risks of recurrence and drug resistance.

Methods: Our previous study has reported WYC-209, which is capable of inducing apoptosis of CSCs in melanoma and hepatoma, but is ineffective against ESCC.

[Retracted] SDF1/CXCR4 axis facilitates the angiogenesis via activating the PI3K/AKT pathway in degenerated discs.

Following the publication of this paper, it was drawn to the Editors' attention by a concerned reader that certain of the Transwell invasion assay data shown in Figs. 2E, 3E, 4E and 5E, and the Transwell migration assay data shown in Fig. 2D, were strikingly similar to data appearing in different form in other articles written by different authors at different research institutes that had either already been published elsewhere prior to the submission of this paper to , or were under consideration for publication at around the same time (some of which have already been retracted).

Identifying and Validating Extracellular Matrix-Related Gene CTSH in Diabetic Foot Ulcer Using Bioinformatics and Machine Learning.

Background: Diabetic foot ulcer (DFU) is a serious clinical problem with high amputation and mortality rates, yet there is a lack of desirable therapy. While the extracellular matrix (ECM) contributes significantly to wound healing, ECM-related biomarker for DFU is still unknown. The study was designed to identify ECM-related biomarker in DFU using bioinformatics and machine learning and validate it in STZ-induced mice models.

A novel compound heterozygous PCDH15 variants is associated with arRP in a Chinese pedigree.

Background: Retinitis pigmentosa (RP) is a heterogeneous group of inherited retinal diseases. However, it is still not well understand about the relationship between PCDH15 variants and RP.

Methods: In this study, we enrolled a Chinese autosomal recessive retinitis pigmentosa (arRP) pedigree and identified the causative gene in the proband by targeted whole exome sequencing (WES).

Mechanism and therapeutic potential of traditional Chinese medicine extracts in sepsis.

Sepsis is a complex syndrome characterized by multi-organ dysfunction, due to the presence of harmful microorganisms in blood which could cause mortality. Complications associated with sepsis involve multiple organ dysfunction. The pathogenesis of sepsis remains intricate, with limited treatment options and high mortality rates.

Investigating Novel Therapeutic Approaches for Idiopathic Short Stature: Targeting siRNA and Growth Hormone Delivery to the Growth Plate Using Exosome Nanoparticles.

Idiopathic short stature (ISS) is a common childhood condition with largely unknown underlying causes. Recent research highlights the role of circulating exosomes in the pathogenesis of various disorders, but their connection to ISS remains unexplored. In the experiments, human chondrocytes are cocultured with plasma exosomes from ISS patients, leading to impaired chondrocyte growth and bone formation.