Safety and Diagnostic Yield of Transbronchial Cryobiopsy by Flexible Bronchoscopy Under Conscious Sedation Without Intubation in Diffuse Parenchymal Lung Disease.

Background: Transbronchial cryobiopsy (TBCB) is generally recommended under intubation or rigid bronchoscopy with general anesthesia. However, some patients with diffuse parenchymal lung disease (DPLD) are unable to tolerate general anesthesia, which limits the widespread adoption of TBCB.

Methods: A total of 37 patients with DPLD who underwent TBCB under conscious sedation without intubation were included in this study.

The level of human epididymis protein 4 is associated with poor prognosis in patients with ejection fraction preserved heart failure.

Introduction: The prognostic value of human epididymis protein 4 (HE4) in patients with heart failure with preserved ejection fraction (HFpEF) is unknown.

Methods: Patients diagnosed with HFpEF in the Department of Cardiovascular Medicine of Hunan Provincial People's Hospital from January 2021 to August 2022 were prospectively enrolled in the study. Serum levels of HE4 were measured using a chemiluminescence microparticle immunoassay.

AIM2-Silenced Dendritic Cells Possess Immunosuppressive Phenotype and Prolong Allograft Survival by Modulating the Th1/Th17/Treg Balance.

Dendritic cells (DCs) serve a pivotal and diverse role in the modulation of transplant immunity, wherein alterations in their maturation state influence the balance between promoting immunotolerance and exacerbating inflammatory responses. Recent studies have revealed a spectrum of novel mechanisms through which absent in melanoma 2 (AIM2) regulates the functions of immune cells. However, the impact of AIM2 on the regulatory functions of dendritic cells in alloimmunity has not been thoroughly investigated.

Integrated analysis of m6A regulator mediated RNA methylation and key immune related genes in ischemic stroke.

Unlabelled: The role of RNA N6-methyladenosine (m6A) modifications in modulating the immune microenvironment during ischemic stroke (IS) pathogenesis remains poorly characterized. This investigation systematically explores m6A-mediated immune regulation in IS and identifies critical immune-related biomarkers. Transcriptomic profiles from 108 IS samples were analyzed to discern m6A regulatory patterns.

Gut microbiota, immune cells, and postpartum depression: A mediation Mendelian randomization study.

Background: Recent studies suggest links between the gut microbiome, immune cells, and postpartum depression (PPD), but causal relationships are unclear. This study employed the Mendelian randomization (MR) to explore the causal relationship and mediating effect among the three factors.

Methods: Summary data from genome-wide association studies were analyzed using MR techniques, including inverse variance weighted, weighted median, MR-Egger, simple mode, and weighted mode.

Rare Case Report of Pulmonary Mucosa-Associated Lymphoid Tissue Lymphoma.

Bronchial mucosa-associated lymphoid tissue is the site of the uncommon malignancy known as mucosa-associated lymphoid tissue (MALT) lymphoma. Due to its lack of distinct clinical signs and imaging characteristics, it is frequently misdiagnosed and underdiagnosed. After receiving unsuccessful treatment for a lung infection or tuberculosis in multiple tertiary care hospitals, we report a male patient who had a solid lesion in his left upper lung for 18 years.

Drug repurposing: isosorbide mononitrate enhances tumor accumulation to augment sonodynamic therapy for hepatocellular carcinoma.

Hepatocellular carcinoma (HCC) remains a leading cause of cancer death worldwide. Sonodynamic therapy (SDT) offers a non-invasive, deep-penetrating approach by using ultrasound to activate sonosensitizers and generate cytotoxic reactive oxygen species (ROS). Yet poor intratumoral delivery and low ROS quantum yields of existing agents have stalled clinical translation.

A common-sense model-based nursing intervention improves exercise compliance in coronary heart disease: a randomized controlled trial and a pilot study.

Aims: This study explored the effects of a Common-sense model of self-regulation-based nursing intervention on enhancing cardiac exercise rehabilitation compliance in coronary heart disease patients, aiming to improve cardiac function and overall health outcomes.

Design: This study was a two-arm, parallel prospective randomized controlled trial.

Methods: Participants were recruited from February to August 2024 at 3 Hospitals in Changsha, Hunan, China.

Latent Profile Analysis of Health Information Literacy in COPD Patients and Its Association with Quality of Life.

Background: Health information literacy (HIL) is crucial to patients' quality of life with chronic obstructive pulmonary disease (COPD). However, individual differences in HIL among COPD patients remain underexplored.

Purpose: This study aimed to determine COPD patients' HIL profiles, investigate the factors influencing these profiles, and assess their association with quality of life (QoL).

Explore the Overall Benefit Index and its correlation with item difficulty among dysphagia-related syndromes in the ICF framework.

Background: Individuals with dysphagia typically experience multiple co-occurring symptoms, whose interrelationships can vary in strength. The significance and impact of each symptom within dysphagia also differ. However, the intricate web of connections among these symptoms remains poorly understood, making it challenging to assess the importance of individual symptoms from a relational perspective.

LINC00313 functions as an oncogenic propellant and immunosuppressive marker in lung adenocarcinoma.

Background: Despite advances in treatments, the prognosis for lung adenocarcinoma (LUAD) remains poor, underscoring the urgent need to identify novel therapeutic strategies. This study was conducted to investigate the potential role of LINC00313 as a biomarker and therapeutic target for LUAD.

Methods: Databases GEPIA2 and GEO were used to analyze LINC00313 expression and prognosis.

[Machine learning-based classification of temporal lobe epilepsy subtypes and surgical prognosis evaluation using PET metabolic networks].

To identify brain metabolic network features for temporal lobe epilepsy (TLE) subtype classification and surgical prognosis prediction using machine learning algorithms, thereby supporting clinical decision-making for TLE subtyping and outcome assessment. ¹⁸F-FDG PET images from 137 patients with drug-resistant TLE treated at Xiangya Hospital's Comprehensive Epilepsy Center from January 2016 to June 2021 were retrospectively analyzed as the training cohort. Network connectivity data were derived using Kullback-Leibler divergence similarity estimation (KLSE), yielding 6 902 network attributes alongside relevant demographic and clinical features.

Steric hindrance engineering to construct NIR-II probe for high-sensitivity G4 imaging .

G-Quadruplexes play key roles in tumorigenesis, but detecting them remains challenging. We developed NIRGQ-3, a NIR-II fluorescent probe, with 25-fold fluorescence enhancement and improved sensitivity (limit of detection: 35 nM). It enables high-contrast tumor imaging (SBR = 25), providing a powerful tool to decipher G-quadruplex dynamics and advancing cancer diagnostics.

Inflammation Inhibits HECTD1-Mediated AURKA Ubiquitination to Cause Extracellular Matrix Degradation in Osteoarthritis via Enhancing Cap-Dependent mRNA Translation of ADAMTS12.

Osteoarthritis (OA) is a common joint disease, and chondrocyte extracellular matrix (ECM) degradation was closely associated with its progression. This study investigated the regulatory mechanisms of ECM degradation during OA development. A rat model of OA was established by anterior cruciate ligament transection (ACL-T) and interleukin-1 beta (IL-1β)-stimulated rat chondrocytes were used to simulate OA in vitro.

Skeletal stem cells, a new direction for the treatment of bone and joint diseases.

Skeletal stem cells (SSCs) are tissue-specific stem cells characterized by their capacity for self-renewal and their position at the apex of the differentiation hierarchy. They can generate mature bone cell types essential for bone development, maintenance, and repair. Lineage tracing experiments have demonstrated that SSCs reside in the bone marrow, periosteum, and the resting zone of the growth plate.

Cryobiopsy as a Salvage Technique Following Negative Flexible Forceps Biopsy of the Pleura Under Rapid On-Site Evaluation Guidance: A Prospective Study.

A diffusely thickened or hard pleura is a special type of macroscopic appearance associated with benign or malignant conditions. Medical thoracoscopy (MT) is the gold standard for pleural pathology, but its diagnostic yield is imperfect. Although cryobiopsy may provide greater and deeper tissue, its impact on the diagnostic yield remains uncertain, and safety concerns persist.

Knowledge, attitudes, and practices of healthcare professionals regarding neuropathic pain in spinal cord injury in Hunan, China.

To evaluate healthcare professionals' knowledge, attitude, and practice regarding spinal cord injury neuropathic pain in Hunan Province, China. This cross-sectional study was conducted between August 2024 and September 2024 in Hunan Province and included healthcare professionals. The questionnaire was self-designed (Cronbach's α = 0.

The Prognostic Value of the XPC rs2228001 Single Nucleotide Polymorphism in Cholangiocarcinoma.

Background And Aims: Cholangiocarcinoma (CCA) is one of the most prevalent primary liver malignancies with increasing incidence and mortality rates, particularly in Southeast Asia. Surgical resection is a primary therapeutic option offered to patients with localised disease. Unfortunately, early disease recurrence is common and robust tools for tailored patient-centric post-operative management and follow-up schemes are highly desired but currently lacking.

Enhanced cosmetic outcomes with central venous catheter drainage in transoral vestibular endoscopic thyroidectomy: a prospective randomized-controlled trial.

With the rising incidence of thyroid cancer, there is an increasing focus on the cosmetic outcomes of thyroid surgery. This study introduces the use of a central venous catheter (CVC) tube as an innovative approach to enhance the aesthetic outcomes of drainage following transoral vestibular endoscopic thyroidectomy. A total of 104 patients were evenly assigned to either the conventional drainage tube group or the CVC tube group.

Targeting the miR-325-3p/GSTP1 axis overcomes paclitaxel resistance in triple-negative breast cancer by inducing ferroptosis.

Background: Triple-negative breast cancer (TNBC) is an aggressive subtype with limited treatment options, and paclitaxel resistance remains a major therapeutic challenge. Ferroptosis, an iron-dependent cell death mechanism, and microRNAs (miRNAs) like miR-325-3p have emerged as potential regulators of chemoresistance, but their roles in TNBC are poorly understood.

Objective: This study aimed to investigate whether ferroptosis and miR-325-3p modulate paclitaxel resistance in TNBC and to identify the underlying molecular mechanisms.

Landscape of Berberine Targets: A Potential Pharmacological Insight for Heart failure Treatment.

Berberine, the primary active compound in Coptis chinensis Franch., is well-known for its anti-infective, hypoglycemic, lipid-lowering, anti-tumor, and anti-inflammatory effects. This review summarizes the physicochemical and pharmacokinetic characteristics of berberine, its intra-intestinal pharmacology involving gut microbiota cross-talk to heart failure (gut-cardiac axis), extraintestinal pharmacology in heart failure, and network pharmacology.

The Effect of Ultrasound-Guided Multipoint Thoracic Paravertebral Nerve Block in Metabolic and Bariatric Surgery (MBS): A Prospective Randomized Controlled Trial.

Background: The study aims to compare the effects of multipoint thoracic paravertebral block combined with general anesthesia to general anesthesia alone in metabolic and bariatric surgery (MBS).

Methods: A total of 80 patients were randomly assigned in a 1:1 ratio to the Thoracic Paravertebral Block group (TPVB group) and the General Anesthesia group (GA group). The TPVB group received multipoint TPVB combined with GA bilaterally at the T6 and T9 levels, while the GA group received only GA.

A General Strategy to Develop Highly Sensitive FAPα Fluorescent Probes for Invasive Cancer Detection.

Fibroblast activation protein α (FAPα) has been reported to be expressed in many carcinoma cells and associated with tumor growth, invasive, and metastasis. However, due to the lack of appropriate fluorescent probes, detecting FAPα with high sensitivity in tumor and clearly delineating tumor margins remains a challenge. Herein, we developed a general design strategy-introducing a positive charge into the fluorophore skeleton-for the construction of FAPα-activated fluorescent probe with enhanced sensitivity.

Primary liver cancer burden and its association with health development in the Western Pacific, 1990-2021.

Background: Liver cancer (LC) is a major public health challenge in the Western Pacific Region (WPR), characterized by etiological diversity and increasing burden from both infectious and noninfectious causes. This study aimed to assess the burden of primary LC and its association with health system development indicators across the WPR from 1990 to 2021.

Methods: We conducted a secondary analysis using data from the Global Burden of Disease (GBD) Study 2021, covering 31 countries and territories in the WPR.