Design, synthesis and biological evaluation of 3,3-dimethyl-2,3,4,9-tetrahydro-1H-carbazole derivatives as AcrB inhibitors with potent antibiofilm effect for reversing bacterial multidrug resistance.

A series of novel 3,3-dimethyl-2,3,4,9-tetrahydro-1H-carbazole derivatives were rationally designed, synthesized and evaluated for their biological activity as AcrB inhibitors. The compounds were assessed for their antibiotic potentiating effects, followed by evaluation of Nile Red efflux inhibition, and off-target effects including activity on the outer and inner bacterial membranes. Ten compounds potentiated antibiotic activity at sub-inhibitory concentrations, reducing the minimum inhibitory concentrations (MICs) of at least one of the tested antibiotics by at least 8-fold, with three derivatives (7c, 11g, and 11i) achieving 32-fold MIC reductions at 128 μg/mL.

Effects of a Comprehensive Intervention Program of Static-Dynamic Balance Training Combined with Reminiscence Therapy on the Level of Healthy Aging Among Older Adults in a Rural Community: A Randomized Controlled Trial.

Purpose: Healthy aging levels are generally low among rural older adults, yet the effects of combined static-dynamic balance training and reminiscence therapy on this population remain underexplored. This study evaluated the efficacy of a combined static-dynamic balance training and reminiscence therapy (SDBT-RT) program in promoting healthy aging in rural communities, with emphasis on both immediate and sustained outcomes.

Patients And Methods: A randomized, assessor-blinded, controlled trial with two parallel groups included 64 adults >60 years from rural Huzhou, China, randomized to intervention (n=32) and control (n=32) groups.

Propolis-derived components act against Klebsiella pneumoniae by inhibiting growth and disrupting biofilms.

Aims: Klebsiella pneumoniae (K. pneumoniae), a multidrug-resistant opportunistic pathogen implicated in pneumonia and nosocomial infections, employs biofilm formation to evade antimicrobial therapies.

Methods: This study investigates how propolis and its key bioactive constituents-naringenin, taxifolin, syringic acid, and gallic acid-disrupt biofilm development and stability in K.

Intraamniotic hemorrhage: challenges in prenatal detection: a case report.

Background: Subamniotic hemorrhage, a rare condition involving bleeding between the amniotic membrane and fetal chorionic plate, presents diagnostic challenges.

Case Presentation: A 35-year-old woman at 37 weeks of gestation who presented with lower abdominal discomfort and decreased fetal movements came to our emergency department. Ultrasound revealed medium-strong echoes in the amniotic fluid and inhomogeneous echoes around the umbilical cord.

Machine learning-guided construction of MoS/MoO heterostructures on hollow carbon shells for polysulfide mitigation in lithium-sulfur batteries.

The commercialization of lithium‑sulfur (LiS) batteries faces fundamental issues from polysulfide shuttling to inefficient redox kinetics, compounded by the absence of systematic methods for catalyst design. Herein, we present a machine learning (ML)-driven strategy to design MoS/MoO heterostructures anchored on nitrogen-doped hollow carbon shells (NCS) via gradient boosting decision trees modeling. The ML-guided optimization identifies critical synthesis parameters (e.

Phenotypic and transcriptomic analysis reveals the antibiofilm mechanism of essential oil against and its application in pork preservation.

is a common foodborne pathogen that can result in diarrhea and vomiting. It can easily exist in food production environments and form biofilms that are difficult to remove. This study investigated the inhibitory effect and mechanism of essential oil (LC-EO) against biofilm based on phenotype and transcriptomics, as well as the application in pork preservation.

Four new species of the lichen genus (Graphidaceae, Ostropales) from Guizhou, China.

Four new species of the lichen genus from China are described based on morphological, chemical, and phylogenetic evidence. Phylogenetic analyses were conducted using both RAxML and Bayesian posterior probability inference, based on combined LSU and mtSSU sequences. The characteristic compounds were analyzed by colorimetric reactions and thin-layer chromatography.

Next-Generation Konjac Glucomannan Hydrogels: Rational Design, Convergent Applications, and Industrial Challenges.

Konjac glucomannan (Konjac gum, KGM), a natural plant-derived polysaccharide, exhibits remarkable biosafety and biodegradability. Its unique rheological properties make it an ideal base material for multifunctional composites. Building on our preliminary findings, this review systematically outlines four fabrication strategies for KGM-based hydrogels: (1) polysaccharide compounding, (2) protein blending, (3) novel resource food compounding, and (4) chemical modification, with particular emphasis on their gelation mechanisms and performance characteristics.

Mechanistic insight into curcumin-induced conjugative plasmid transfer acceleration: Role of intracellular arginine uptake.

Curcumin exhibits a broad spectrum of applications spanning multiple domains, including its incorporation in dietary supplements, functional beverages, cosmetic formulations, and nutraceutical products. Nevertheless, its potential influence on the development of antibiotic resistance remains to be fully elucidated. Therefore, this study aims to investigate the effects of curcumin on the conjugative transfer of plasmids carrying antibiotic resistance genes (ARGs).

Combining knowledge distillation and neural networks to predict protein secondary structure.

The secondary structure of a protein serves as the foundation for constructing its three-dimensional (3D) structure, which in turn is critical for determining its function and role in biological processes. Therefore, accurately predicting secondary structure not only facilitates the understanding of a protein's 3D conformation but also provides essential insights into its interactions, functional mechanisms, and potential applications in biomedical research. Deep learning models are particularly effective in protein secondary structure prediction because of their ability to process complex sequence data and extract meaningful patterns, thereby increasing prediction accuracy and efficiency.

11-Methoxytabersonine Induces Ferroptosis in Triple-Negative Breast Cancer via YY1-CDKL1-GPX4 Axis.

The 11-Methoxytabersonine (11-MT), a monoterpenoid-indole alkaloid isolated from the leaves of Melodinus henryi, has shown promising therapeutic potential against triple-negative breast cancer (TNBC). This study aimed to evaluate the anti-tumor efficacy of 11-MT and to elucidate its underlying molecular mechanisms in the context of TNBC. The in vitro anti-cancer effects of 11-MT were assessed using Cell Counting Kit-8 (CCK-8), colony formation assays, and flow cytometry.

Design, Synthesis and Herbicidal Activity of 5-(1-Amino-4-phenoxybutylidene)barbituric Acid Derivatives Containing an Enamino Diketone Motif.

In continuation of our efforts to identify novel herbicide lead compounds, twenty new 5-(1-amino-4-phenoxybutylidene)barbituric acid derivatives containing an enamino diketone motif were synthesized and evaluated for their herbicidal activities. The greenhouse bioassay results indicated that several of the target compounds, including , , , , and , exhibited notable post-emergence herbicidal activity, with sum inhibition rates exceeding 70% at a dosage of 150 g ha, which was superior to that of the commercial herbicide flumiclorac-pentyl (FP). The structure-activity relationship analysis demonstrated that the steric and electronic effects of the R group, as well as the lipophilicity of the target compounds, significantly influenced herbicidal activity.

Design, Synthesis, and Pharmacological Activity of the -(4-(1H-1,2,4-Triazol-1-yl)phenyl)-substituted-amide Derivatives.

To discover novel, potent anticonvulsant compounds, a series of compounds containing triazole structural fragments were synthesized and evaluated for their anticonvulsant activity. Compounds (maximal electroshock (MES), 50% effective dose (ED) = 13.1 mg/kg; subcutaneous pentylenetetrazole (scPTZ), ED = 19.

Antimicrobial Activities of Propolis Nanoparticles in Combination with Ampicillin Sodium Against Methicillin-Resistant .

Combining antibiotics with propolis is an effective method to combat bacterial drug resistance. Nanoparticles are of interest in the antimicrobial field because of their higher drug stability, solubility, penetration power, and treatment efficacy. In this study, propolis nanoparticles (PNPs) were synthesized, and their antibacterial and anti-biofilm activities against methicillin-resistant (MRSA) in combination with ampicillin sodium (AS) were analyzed.

Preparation of -Derived Immune Modulators and Alleviation of Cyclophosphamide-Induced Immune Suppression and Intestinal Microbiota Dysbiosis in Mice.

With the acceleration of the pace of life, increased stress levels, and changes in lifestyle factors such as diet and exercise, the incidence of diseases such as cancer and immunodeficiency has been on the rise, which is closely associated with the impaired antioxidant capacity of the body. Polypeptides and polysaccharides derived from edible fungi demonstrate significant strong antioxidant activity and immunomodulatory effects. , the second most cultivated mushroom in China, is not only nutritionally rich but also offers considerable health benefits.

Research Progress on the Mechanism of Action of Food-Derived ACE-Inhibitory Peptides.

Hypertension is a major pathogenic contributor to cardiovascular diseases, primarily mediated through activation of the angiotensin-converting enzyme (ACE) system. Food-derived ACE-inhibitory peptides represent a promising alternative to synthetic drugs due to their favorable safety profile and minimal side effects. ACE-inhibitory peptides have been extensively identified from various foods, with their antihypertensive activity and molecular mechanisms comprehensively characterized through in vitro and in vivo studies.

Transcriptomic Analysis Reveals Regulatory Responses of Fatty Acid Positional Distribution in Triacylglycerols and Lipid Composition to Dietary n-3 HUFA in the Muscle of .

The nutritional value of lipids depends not only on their fatty acid composition but also on their stereospecific positioning on the glycerol backbone. This study investigated the fatty acid composition and -2 positional distribution of triacylglycerols (TAG), as well as the composition of major phospholipids in golden pompano () juveniles (initial weight: 10 g) fed five diets including graded levels of dietary n-3 highly unsaturated fatty acids (HUFA; 0.64-2.

A narrative review of deep learning applications in sports performance analysis: current practices, challenges, and future directions.

Background: The integration of deep learning techniques into sports performance analysis has significantly advanced athlete monitoring, motion tracking, and predictive modelling. These advancements have significantly improved the ability to assess performance, optimize training strategies, and reduce injury risks. However, despite notable progress, challenges remain in standardizing methodologies, ensuring model reliability, and enhancing real-time application across various sports disciplines.

Molybdenum-Induced Oxidative and Inflammatory Injury and Metabolic Pathway Disruption in Goat Pancreas.

: Molybdenum (Mo) is an essential trace element for animals, but too much intake can cause adverse effects. Due to the metabolic characteristics of goats and other ruminants, they are more susceptible to the cumulative effects of Mo toxicity. A high Mo intake can cause multi-organ toxicity in ruminants, but the mechanism of damage to the pancreas is still unclear.

Annual Dynamic Changes in Lignin Synthesis Metabolites in 'Jinsi'.

: 'Jinsi' has excellent wood properties and golden texture, which is widely used in producing furniture and crafts. The lignin content and structural composition often determine the use and value of wood. Hence, investigating the characteristics of the annual dynamics of lignin anabolic metabolites in 'Jinsi' and analyzing their synthesis pathways are particularly important.

Research on Path Planning Method for Mobile Platforms Based on Hybrid Swarm Intelligence Algorithms in Multi-Dimensional Environments.

Traditional algorithms such as Dijkstra and APF rely on complete environmental information for path planning, which results in numerous constraints during modeling. This not only increases the complexity of the algorithms but also reduces the efficiency and reliability of the planning. Swarm intelligence algorithms possess strong data processing and search capabilities, enabling them to efficiently solve path planning problems in different environments and generate approximately optimal paths.

Correction: The composition and predictive function of the fecal microbiota in female donkeys across different reproductive cycles.

[This corrects the article DOI: 10.3389/fmicb.2025.

Recombinant Escherichia coli biotransformation of biliverdin to synthesize bilirubin.

Bilirubin, a heme-derived metabolite with pronounced bioactivity, exhibits significant potential for pharmaceutical applications. This study developed a recombinant Escherichia coli-based biliverdin biotransformation system, enabling efficient bilirubin synthesis via heterologous expression of a novel biliverdin reductase (BvdR', XP_032760038.1) from Rattus norvegicus and a glucose dehydrogenase (GDH) from Priestia megaterium.

The emerging role of milk-derived extracellular vesicles in gut pathology and cancer management.

Background: Milk extracellular vesicles (mEVs) are emerging as important mediators in gut pathology and cancer management. These stable nanoscale vesicles contain bioactive cargos including microRNAs, proteins, and lipids that facilitate intercellular communication and offer therapeutic opportunities.

Scope And Approach: This review examines mEVs' role in gut health and cancer therapy, synthesizing recent evidence from 2021 to 2025 across various milk sources (camel, bovine, equine, ovine, human).

Synergistic tri-site NiZnAl catalyst for aqueous-phase hydrogenation and cascade rearrangement of furfural to cyclopentanone.

The tandem catalytic conversion of furfural to cyclopentanone represents a crucial transformation in biomass valorization, yet faces persistent challenges due to competing hydrogenation and ring-opening pathways. Herein, we report the rational design of a three-dimensional flower-like hierarchical nanostructure catalyst derived from NiZnAl-layered double oxide precursors, which features well-dispersed NiZn alloy active sites. Comprehensive characterization studies reveal that Zn incorporation plays a dual role in modulating surface acidity and promoting oxygen vacancies formation through structural reconstruction.