[Exploration of ribosomal DNA copy number as an early biomarker for exposure to chemical carcinogens].

As the relationship between exposure to chemical carcinogens and human health risks becomes increasingly clear, identifying effective biomarkers to reveal early exposure and prevent the occurrence and development of tumors has become particularly critical. Ribosomal DNA (rDNA), a cluster of genes encoding ribosomal RNA, has emerged as a focal point in chemical carcinogen exposure research in recent years, due to its high copy number within cells and sensitivity to environmental stimuli. The dynamic changes in rDNA copy numbers, especially the significant alterations observed following exposure to environmental carcinogens, have rendered it a promising potential biomarker.

Highly sensitive and selective ratiometric fluorescence detection of ATP using aptamer-functionalized FeO nanozymes with tunable activity.

Herein, a highly sensitive and selective ratiometric fluorescence platform was constructed for probing adenosine triphosphate (ATP) for the first time by using magnetic FeO nanozymes that were modified by ATP-specific aptamer labeled with 7-Amino-4-methyl-3-coumarinylacetic acid (Apt-AMCA) to yield FeO@Apt-AMCA. It was discovered that the incorporation of Apt-AMCA greatly enhance the peroxidase-like activity of FeO nanozymes, which might catalyze more o-phenylenediamine (OPD) substrates into fluorescent oxidized OPD (DAP) with a emission of 560 nm. More importantly, upon ATP addition, Apt-AMCA could potentially be released from FeO@Apt-AMCA because of Apt-target binding and the competition between ATP and Apt.

Effects of a Comprehensive Intervention Program of Static-Dynamic Balance Training Combined with Reminiscence Therapy on the Level of Healthy Aging Among Older Adults in a Rural Community: A Randomized Controlled Trial.

Purpose: Healthy aging levels are generally low among rural older adults, yet the effects of combined static-dynamic balance training and reminiscence therapy on this population remain underexplored. This study evaluated the efficacy of a combined static-dynamic balance training and reminiscence therapy (SDBT-RT) program in promoting healthy aging in rural communities, with emphasis on both immediate and sustained outcomes.

Patients And Methods: A randomized, assessor-blinded, controlled trial with two parallel groups included 64 adults >60 years from rural Huzhou, China, randomized to intervention (n=32) and control (n=32) groups.

Broad-spectrum antitumor analysis of the telomerase activity inhibitor TPCH derived from the human constitutively expressed protein LPTS/PinX1.

Background: The human liver-related putative tumor suppressor LPTS/PinX1 is a gene encoding a telomerase inhibitory protein. Overexpression of LPTS/PinX1 protein can inhibit the growth of multiple telomerase-positive cancer cell lines. LPTS/PinX1 has therapeutic potential for cancer.

Manganese catalyzed cross-coupling of allylic alcohols and indoles: an elegant route for access to γ-hydroxyindole.

Alcohol and indole derivatives are widely recognized as versatile building blocks in both chemical and biochemical synthesis. The formal conjugate addition between these two classes of compounds provides a powerful and sustainable strategy for constructing γ-hydroxyindoles, owing to the reaction's 100% atom economy and the ready availability of starting materials. In this study, we report a redox-neutral cross-coupling reaction between indoles and allylic alcohols, catalyzed by a manganese(i) pincer complex, which enables the efficient synthesis of a broad range of γ-hydroxyindoles under mild conditions.

Genomic Investigation of a Bacillus subtilis Strain Sourced from Commercially Available Milk Powder in China Reveals Potential Risk Factors.

Background: Milk powder is a key food source, especially for infants and vulnerable groups. However, Bacillus contamination during production, storage, or handling can cause spoilage, quality issues, or health risks. This study identified and isolated from commercially available Chinese milk powder.

Transcutaneous Auricular Vagus Nerve Stimulation for Postpartum Contraction Pain During Elective Cesarean Delivery: A Randomized Clinical Trial.

Importance: Postpartum uterine contraction pain manifests as a severe, intermittent lower abdominal discomfort caused by uterine contraction during the early puerperium. Transcutaneous auricular vagus nerve stimulation (taVNS) has demonstrated promising effects in managing both acute and chronic pain conditions; however, there remains a paucity of studies regarding the use of taVNS for alleviating postpartum uterine contraction pain.

Objective: To investigate the effect of taVNS in alleviating postpartum uterine contraction pain among women receiving combined spinal-epidural anesthesia for elective cesarean delivery.

Does the trade of medical products contribute to promoting human development?-An empirical analysis based on data from China and RCEP countries.

Background: As the RCEP (Regional Comprehensive Economic Partnership) Agreement deepens, the role of medical product trade in safeguarding regional public health security and promoting economic development has become increasingly prominent.

Methods: Based on data from 2004 to 2023, this study employs the stochastic frontier gravity model and benchmark regression model to explore the impact of China's medical product export efficiency on the development indices of RCEP countries.

Results: The findings are as follows: (1) China's medical product exports to RCEP countries exhibit simultaneous scale expansion, structural differentiation, and market concentration, with ASEAN, Japan, and South Korea as core markets and mid-to-low-end products dominating the export structure; (2) Trade efficiency evolution reflects dual mechanisms of institutional environment and technological capabilities, where governance levels and health system soundness optimize efficiency, while excessive liberalization and tax burdens increase friction costs; (3) Enhanced export efficiency indirectly promotes human development in RCEP countries by strengthening basic health service coverage, forming a "trade-health-development" transmission mechanism.

Deep learning-based regression of food quality attributes using near-infrared spectroscopy and hyperspectral imaging: A review.

Near-infrared (NIR) spectroscopy and hyperspectral imaging (HSI) are two popular non-destructive tools for food quality and safety inspection. For food quality attributes quantification, the key is to develop regression models to link the features (spectral, spatial and their fusion) and the quality attributes. In addition to conventional machine learning methods, deep learning-based regression has proved to be a promising and advantageous approach to quantify the quality attributes.

Effect of transcutaneous auricular vagus nerve stimulation on postoperative liver function in patients undergoing partial hepatectomy: a study protocol for a prospective, double-blind, randomized controlled trial.

Background: Partial hepatectomy remains a primary therapeutic intervention for various hepatic diseases. However, several intraoperative factors, including surgical manipulation, substantial blood loss, the need for blood transfusions, and hypoxic stress, can significantly impair liver function. Current perioperative strategies aimed at protecting the liver exhibit certain limitations.

Enhancement or Activation of Agricultural Antibiotic Production in by an Identified Strong Promoter P.

We first screened for strong constitutive promoters by analyzing transcriptomics data under various fermentation conditions, focusing on genes with sustained high expression levels. Then, we validated the expression strength of 21 candidate promoters using β-glucuronidase enzymatic activity and qRT-PCR assays and observed that promoter P demonstrated consistently strong expression and efficacy in M527. The higher expression level and utility of P were further confirmed in three different host strains, including the industrial strain 1628.

Prenatal exposure to organophosphorus flame retardants and birth outcomes: A cross-sectional study in an e-waste area in China.

Organophosphorus flame retardants (OPFRs), widely adopted as substitutes for restricted brominated flame retardants (BFRs), have emerged as significant environmental contaminants in regions burdened by electronic waste (e-waste) recycling activities. Consequently, this study aimed to investigate the relationship between prenatal exposure to OPFRs and infant birth outcomes in an e-waste area. We used programmed-temperature vaporizer gas chromatography-mass spectrometry (PTV-GC/MS) to measure the concentrations of thirteen OPFRs in umbilical cord blood samples from 131 mother-infant pairs in Taizhou.

A model based on preoperative nutrition-inflammation score for predicting mucocutaneous separation after enterostomy in colorectal cancer patients.

Mucocutaneous separation (MCS) is a common early complication after colorectal cancer (CRC) surgery. The aim of the present study is to investigate the predictive value of preoperative nutrition-inflammation markers for MCS and establish a novel predictive model. The internal cohort composed of CRC patients admitted to Changhai Hospital Affiliated to Naval Medical University was randomly divided into a training cohort and an internal validation cohort at a ratio of 7:3, while patients from the First Affiliated Hospital of Bengbu Medical University and 72nd Group Army Hospital formed an external cohort.

Dissolving microneedles enabled delivery of Oxaliplatin- sodium butyrate loaded outer membrane vesicles against rectal cancer.

Background: Oxaliplatin (OXA) is a commonly used drug for the treatment of rectal cancer (RC). However, traditional administration methods are plagued by low efficiency, high systemic side effects, and poor patient tolerance. Therefore, there is an urgent need to develop a targeted drug delivery system to enhance efficacy and reduce toxicity.

Effects of inter-group contact training on aging-related stereotypes among undergraduate nursing students: a randomized controlled trial.

Strategies to build a larger workforce of geriatric care providers are needed. One method to address the shortage of geriatric care providers is inter-group contact training to improve aging-related stereotypes among undergraduate nursing students, who are an important reserve for geriatric care. But studies on inter-group contact training for nursing students and nurses are limited.

Correction: Dual roles of proteins in macrophage immune dynamics.

[This corrects the article DOI: 10.3389/fimmu.2025.

Uncovering the gaps: a grounded theory approach to conceptualizing inadequate child family caregiving in China.

Objective: This study investigates the manifestations of inadequate family caregiving for children in China and aims to construct a conceptual framework that captures the multifaceted nature of this phenomenon within a rapidly changing social context.

Methods: Based on constructivist grounded theory, in-depth interviews were conducted with 28 children and their primary family caregivers across diverse socioeconomic backgrounds, selected through purposive and theoretical sampling. Through open, axial, and selective coding, this study identified and refined the core attributes, antecedents, and outcomes of inadequate family caregiving, ultimately developing a conceptual framework.

Ginsenoside Rb1 ameliorates post-doxorubicin treatment myocardial hypertrophy via CaN/NFATc4/GATA4.

Background: Myocardial hypertrophy is a crucial pathological change that occurs during post-anthracycline treatment cardiomyopathy. The effects of ginsenoside Rb1 (Rb1) on anthracycline-induced hypertrophy remain unclear. This study aimed to explore the antihypertrophic effect of Rb1 on post-doxorubicin (DOX) treatment myocardial hypertrophy and underlying mechanism.

Photocatalytic CO-releasing spray hydrogel for in situ postoperative cancer treatment.

The high invasiveness and metastatic potential of breast cancer increase the risk of postoperative recurrence. To address this issue, a composite hydrogel drug delivery system based on sodium alginate (SA) has been developed for the in situ release of carbon monoxide (CO) at the tumor resection site. This system aims to enhance the effectiveness of chemotherapy and improve the clearance of residual tumor cells after surgery, thereby preventing tumor recurrence and metastasis.

Development and validation of screening tools for motoric cognitive risk syndrome in community settings.

Background: Motoric cognitive risk (MCR) syndrome represents an "ultra-early" stage of dementia prevention, highlighting the need for effective screening tools.

Aim: To develop and validate a novel tool for MCR identification, comparing its effectiveness with existing methods.

Methods: As part of a community study on healthy aging, a cross-sectional study recruited 1189 Chinese participants aged 50 years and older between May 1, 2022, and March 15, 2023.

Application and efficacy of quetiapine in patients with high-risk factors for bipolar disorder: A randomized controlled trial.

Background: Bipolar disorder (BD), marked by recurring manic and depressive episodes, often coexists with anxiety disorder (AD), which increases treatment complexity and morbidity. Although quetiapine, an atypical antipsychotic, has demonstrated efficacy in treating BD and AD, further investigation is needed regarding its effectiveness and safety in patients with AD at high-risk factors for BD.

Aim: To explore the application and efficacy of quetiapine in combination therapy for patients with AD at high-risk factors for BD.

Effects of moderate intensity continuous training combined with emotional freedom techniques on fatigue in patients with chronic obstructive pulmonary disease: A four-arm parallel randomized controlled trial.

Fatigue is one of the most common and distressing symptoms in patients with chronic obstructive pulmonary disease (COPD). This study applied MICT, EFT and their combination (MICT and EFT) to COPD patients with fatigue through factorial design analysis method, aiming to explore non-drug management strategies that are more conducive to relieving fatigue in COPD patients. This study had a total of 112 participants.

Effects of different exercises on health-related physical fitness among middle-aged and elderly women.

Objective: To explore the effects of long-term exercise of Tai Chi Chuan, walking, and Jiamusi gymnastics on the health-related factors of physical fitness among middle-aged and elderly women.

Methods: Long-term exercise Tai Chi Chuan group, walking group, Jiamusi gymnastics group, and non-exercise group were used to measure their health-related physical fitness, balance, and life satisfaction through long-term exercise Tai Chi Chuan group, walking group, Jiamusi gymnastics group, and non-exercise group matched by age, height, and weight.

Results: Compared with control group, Tai Chi Chuan were significant differences in all indicators, except systolic blood pressure (SBP), diastolic blood pressure (DBP), resting heart rate (RHR), and life satisfaction ( < 0.

A new glycosylated derivative of rausuquinone with cytotoxic activity from Streptomyces sp. HU061-2.

A new glycosylated derivative of rausuquinone, rausuquinonoside (1), was isolated from the Tai Lake sediment-derived actinomycete, Streptomyces sp. HU061-2. The structure of 1 was determined by 1D, 2D NMR, MS spectral analysis and the comparison with data from the literature.