Emergence of transmissible plasmids in clinical : CRISPR typing unravels phage-driven evolution and high-risk lineage.

spp. is an important foodborne pathogen that causes serious neonatal infections. The prevalence of the colistin resistance gene in spp.

Transcutaneous Auricular Vagus Nerve Stimulation for Postpartum Contraction Pain During Elective Cesarean Delivery: A Randomized Clinical Trial.

Importance: Postpartum uterine contraction pain manifests as a severe, intermittent lower abdominal discomfort caused by uterine contraction during the early puerperium. Transcutaneous auricular vagus nerve stimulation (taVNS) has demonstrated promising effects in managing both acute and chronic pain conditions; however, there remains a paucity of studies regarding the use of taVNS for alleviating postpartum uterine contraction pain.

Objective: To investigate the effect of taVNS in alleviating postpartum uterine contraction pain among women receiving combined spinal-epidural anesthesia for elective cesarean delivery.

Effect of transcutaneous auricular vagus nerve stimulation on postoperative liver function in patients undergoing partial hepatectomy: a study protocol for a prospective, double-blind, randomized controlled trial.

Background: Partial hepatectomy remains a primary therapeutic intervention for various hepatic diseases. However, several intraoperative factors, including surgical manipulation, substantial blood loss, the need for blood transfusions, and hypoxic stress, can significantly impair liver function. Current perioperative strategies aimed at protecting the liver exhibit certain limitations.

RNA-viromics unveils diverse RNA viral communities in Large-billed crows and Northern Ravens.

Birds have historically served as key vectors for viruses causing significant diseases. Corvid birds, often living in close proximity to livestock, poultry, and humans, provide substantial opportunities for cross-species viral transmission. Such transmission can occur through their feces or via ectoparasites (such as ticks, mites, and fleas) on their bodies, thereby releasing viruses into the environment.

The Effect of Frailty on Prognosis of Patients Undergoing Abdominal Aortic Aneurysm Repair: A Systematic Review and Meta-Analysis.

Background: This study aimed to systematically review the impact of frailty on outcomes following abdominal aortic aneurysm (AAA) surgery.

Methods: MEDLINE, Embase, and Scopus were searched for studies of frailty on AAA surgery. A modified Newcastle-Ottawa scale was used to assess the quality of the included studies.

Exosomes derived from human umbilical cord mesenchymal stem cells ameliorate AKI after cardiac surgery by facilitating miR-21-5p targeting TEAD1.

Background: Acute kidney injury (AKI) is a common complication of cardiac surgery. Human umbilical cord mesenchymal stem cell (hUCMSC)-derived exosomes affect tissue damage and repair, and miR-21-5p reduces apoptosis and inflammation. However, miR-21-5p's role in cardiac surgery remains unclear.

Theaflavin inhibits the malignant phenotype of human anaplastic thyroid cancer 8305C cells by regulating lipid metabolism via PI3K/AKT signaling.

Background: Anaplastic thyroid cancer (ATC) is a rare but extremely aggressive type of thyroid cancer derived from follicular cells of the thyroid gland. Due to the rapid growth of neck masses in ATC patients, which can lead to asphyxia, multimodality therapy, including surgery, chemotherapy, and radiotherapy, is essential in the treatment of ATC. Theaflavin (TF) has anti-cancer effects; however, no study has examined whether TF inhibits the malignant progression of ATC.

Anthropometric Measures, Recurrent Laryngeal Nerves Diameter, Electromyographic Responses and Vocal Cord Paralysis Risk.

Objectives: To examine (1) the relationship between anthropometric measurements and recurrent laryngeal nerve (RLN) diameter; (2) whether thin RLNs have different baseline electromyographic (EMG) characteristics; (3) if thin or branched morphology is associated with increased risk of EMG adverse events, loss of signal (LOS) or vocal cord paresis/paralysis (VCP).

Methods: In this prospective study, anthropometric data were collected, including weight, height, body mass index (BMI), neck circumference, shoulder diameter, and circumference of the right middle finger (RMF) or right ring finger (RRF).

Results: We enrolled 216 patients who underwent surgery from 2016 to 2020 with 307 RLNs at risk.

Prognostic Role of Radiotherapy in Low-Grade Endometrial Stromal Sarcoma: A SEER-Based Study.

IntroductionLow-grade endometrial stromal sarcoma (LG-ESS) is a rare malignant mesenchymal neoplasm for which there is no consensus regarding the role of radiotherapy in treatment. This study aimed to evaluate the prognostic significance of external beam radiotherapy (EBRT) using data from the Surveillance, Epidemiology, and End Results (SEER) database.MethodsThis retrospective study evaluated the role of EBRT in 1254 patients with LG-ESS using SEER data (2000-2021).

The diagnostic value of serum C-reactive protein/albumin and homocysteine/high-density lipoprotein-cholesterol in coronary microvascular angina pectoris.

Objective: The aim of this study was to investigate the correlation between the serum C-reactive protein/albumin ratio and homocysteine/high-density lipoprotein-cholesterol ratio with microvascular angina and assess their predictive value.

Methods: A total of 70 patients diagnosed with microvascular angina comprised the observation group, while 68 patients with normal or minimal (<50%) coronary stenosis and normal coronary blood flow formed the control group. Serum C-reactive protein, albumin, homocysteine, high-density lipoprotein-cholesterol, and other indexes were measured, and C-reactive protein/albumin and homocysteine/high-density lipoprotein-cholesterol ratios were calculated.

Elucidating the chromatin-driven transcription regulatory networks response to Streptococcus agalactiae infection under low temperature in Nile tilapia.

Low temperature impairs immune function in ectothermic vertebrates, but the underlying regulatory mechanisms remain unclear. Here, we show that hypothermia significantly decreases bacterial load and increases mortality in Nile tilapia (Oreochromis niloticus) following Streptococcus agalactiae infection. Integrative RNA-seq and ATAC-seq profiling of head kidney tissue revealed widespread suppression of immune gene expression under cold stress, including ifrd2, isg15, cxcr1, ccr2, ccr9a, and socs1b.

Combined effects of polyamide microplastics and the pathogenic bacterium Vibrio parahaemolyticus on the immune parameters of Mytilus coruscus.

The thick-shelled mussel, Mytilus coruscus, plays a vital role in marine ecosystems but is vulnerable to pollutants. Polyamide microplastics (PA-MPs), such as nylon, can adsorb harmful bacteria like Vibrio parahaemolyticus, posing threats to bivalves and economic risks to aquaculture. In this study, M.

Large Language Models and Artificial Neural Networks for Assessing 1-Year Mortality in Patients With Myocardial Infarction: Analysis From the Medical Information Mart for Intensive Care IV (MIMIC-IV) Database.

Background: Accurate mortality risk prediction is crucial for effective cardiovascular risk management. Recent advancements in artificial intelligence (AI) have demonstrated potential in this specific medical field. Qwen-2 and Llama-3 are high-performance, open-source large language models (LLMs) available online.

Metagenomic Insights into the Diverse Antibiotic Resistome of Non-Migratory Corvidae Species on the Qinghai-Tibetan Plateau.

Antibiotic resistance represents a global health crisis with far-reaching implications, impacting multiple domains concurrently, including human health, animal health, and the natural environment. Wild birds were identified as carriers and disseminators of antibiotic-resistant bacteria (ARB) and their associated antibiotic resistance genes (ARGs). A majority of studies in this area have concentrated on migratory birds as carriers for the spread of antibiotic resistance over long distances.

Transcriptional regulation by PHGDH drives amyloid pathology in Alzheimer's disease.

Virtually all individuals aged 65 or older develop at least early pathology of Alzheimer's disease (AD), yet most lack disease-causing mutations in APP, PSEN, or MAPT, and many do not carry the APOE4 risk allele. This raises questions about AD development in the general population. Although transcriptional dysregulation has not traditionally been a hallmark of AD, recent studies reveal significant epigenomic changes in late-onset AD (LOAD) patients.

Diagnostic value and regulatory role of long non-coding RNA MIR210HG in hypertension.

Background: Hypertension is the most prevalent chronic disease globally and the treatment and control ratio of hypertension patients are still low. The function of lncRNA MIR210HG in hypertension has not yet been announced.

Methods: Eighty-three hypertension patients and 79 healthy individuals were enrolled.

The correlation between muscle loss and the severity of vascular stenosis in elderly patients with peripheral artery disease: a retrospective analysis utilizing computed tomography.

Background: Peripheral artery disease (PAD) is a globally prevalent atherosclerotic disease associated with an increased risk of cardiovascular and cerebrovascular diseases and a poor prognosis. Skeletal muscle loss (sarcopenia) is particularly common in patients with PAD and is closely associated with poor prognosis.

Aims: The aim of this study was to evaluate the area, density and fat infiltration of skeletal muscle in patients with PAD by CT, and to analyze their relationship with the degree of vascular stenosis.

Monitoring insecticide resistance and target-site mutations in field populations of Spodoptera frugiperda (Lepidoptera: Noctuidae) in China.

The fall armyworm Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), a notorious invasive pest, has been widely monitored for insecticide resistance.

Adaptive and Robust Switchable Adhesion System: Bio-Inspired Synergistic Integration from Octopuses and Geckos.

Existing climbing robots achieve stable movements on limited surface types. However, adapting a single robot design to various surface shapes remains a substantial challenge. Based on the van der Waals (vdW) force-mediated adhesion mechanism of a gecko foot and negative pressure from octopus suckers, this study introduces a biomimetic integration strategy for designing and fabricating a pneumatically actuated switchable adhesion system (SAS).

Discovery, structural characteristics and evolutionary analyses of functional domains in Acinetobacter baumannii phage tail fiber/spike proteins.

Background: The global rise in multidrug-resistant Acinetobacter baumannii infections poses a significant healthcare challenge. Bacteriophage offer a promising alternative to antibiotics for treating A. baumannii infections.

The combination of rAAV pseudo-lipid nanoparticle and triamcinolone acetonide enables multi-administration to liver.

The multi-administration of recombinant adeno-associated virus (rAAV) is limited largely by immunological barriers. Herein, a novel strategy, named rAAV pseudo-lipid nanoparticle combined with triamcinolone acetonide (LNP-rAAV + TAC), has been described in mice. We showed successful but low efficient triple trafficking by LNP-rAAV2 carrying EGFP, human factor IX (hFIX), and luciferase (luc), due to its encapsulation characteristic.

Machine Learning Analysis of Nutrient Associations with Peripheral Arterial Disease: Insights from NHANES 1999-2004.

Background: Peripheral arterial disease (PAD) is a common manifestation of atherosclerosis, affecting over 200 million people worldwide. The incidence of PAD is increasing due to the aging population. Common risk factors include smoking, diabetes, and hyperlipidemia, but its exact pathogenesis remains unclear.

Clinical significance of retrograde inferior parathyroid protection technique based on thymus preservation in thyroid surgery.

Background: The importance of parathyroid gland preservation in thyroid surgery has been well recognized; however, the rapid identification of the parathyroid gland, particularly the inferior parathyroid gland (IPG), remains challenging. This study aimed to evaluate the effectiveness of retrograde inferior parathyroid protection technique (RIPPT) based on thymus preservation.

Methods: A total of 236 patients were enrolled in this study between August 2019 and December 2020.