Machine learning-enhanced digital microscopy for personalized assessment of red blood cell storage lesions.

Background And Objectives: This study aims to develop a novel platform combining machine learning and microscope images for personalized assessment of red blood cell (RBC) storage lesions. RBCs undergo storage lesions, which adversely affect transfusion outcomes. Currently, there is no individualized assessment method for RBC aging applicable in clinical practice.

Genome-wide discovery of circulating cell-free DNA methylation signatures for the differential diagnosis of triple-negative breast cancer.

Background: Preoperative identification of breast cancer (BC) subtypes is essential for optimizing treatment strategies and improving patient outcomes. This study aimed to identify circulating cell-free DNA (cfDNA) methylation signatures to differentiate triple-negative breast cancer (TNBC) from other BC subtypes (non-TNBC).

Methods: We initially performed a genome-wide analysis to identify differentially methylated CpG sites (DMCs; |Δ| > 0.

Robot-assisted versus conventional percutaneous screw fixation in calcaneal fractures: a retrospective analysis.

Purpose: This study aimed to evaluate the impact of robotic guidance on screw placement accuracy and clinical outcomes in patients with calcaneal fractures. The primary research question was whether robot-assisted fixation provides superior surgical precision and recovery compared to conventional percutaneous techniques.

Methods: A retrospective review of 98 patients treated between April 2022 and March 2024 was conducted.

Fresh human neck dissection specimens as vascular models for head and neck microsurgery training.

Background: Microsurgical techniques have advanced across various fields, requiring high precision, accuracy, and minimal invasiveness. While existing animal and cadaver models offer training opportunities for surgeons to practice surgeries, they struggle to accurately replicate real surgical scenarios.

Methods: This study developed microsurgery training models using discarded human neck dissection specimens, with latex perfusion for dissection models and synthetic blood for anastomosis models.

Reduced Antarctic Bottom Water overturning rate during the early last deglaciation inferred from radiocarbon records.

The rapid CO rise during the early deglaciation is often linked to enhanced ventilation by intensified Antarctic Bottom Water (AABW) overturning. The recorded radiocarbon ventilation seesaw during the early deglaciation, which describes improved Southern Ocean and reduced North Atlantic abyssal radiocarbon ventilation, has been interpreted as intensified AABW and reduced North Atlantic Deep Water convections. However, abyssal radiocarbon records also reflect changes in surface reservoir ages and interior water mass mixing.

Identification of Potential Urine Biomarkers of Hypertensive Nephropathy for Predicting Disease Progression Based on Metabolomics and Peptidomics.

Hypertensive nephropathy (HN) complicates hypertension with subtle early symptoms, hindering diagnosis. To address this, an integrated urinary metabolomics and peptidomics analysis was conducted comparing HN patients to hypertensive (HTN) controls, aiming to identify molecular signatures indicative of disease progression and clarify the pathophysiological mechanisms driving HN development. Urine samples were analyzed from both discovery (51 HTN vs 51 HN patients, including 27 early stage and 24 advanced-stage) and validation (21 HTN vs 21 HN) cohorts.

Clathrin Light Chain B Drives Hepatocellular Carcinoma Progression Through Dual Mechanisms: Small Extracellular Vesicle-Mediated Angiogenesis and the NF-κB-PCLAF Signaling Axis.

Clathrin light chain B (CLTB) is one of the three light chain subunits of the clathrin complex. This study aims to elucidate the role of CLTB in the pathogenesis of hepatocellular carcinoma (HCC) and its clinical implications. Clinical and bioinformatic analyses reveal marked CLTB overexpression in HCC tissues.

The Effect of Frailty on Prognosis of Patients Undergoing Abdominal Aortic Aneurysm Repair: A Systematic Review and Meta-Analysis.

Background: This study aimed to systematically review the impact of frailty on outcomes following abdominal aortic aneurysm (AAA) surgery.

Methods: MEDLINE, Embase, and Scopus were searched for studies of frailty on AAA surgery. A modified Newcastle-Ottawa scale was used to assess the quality of the included studies.

DNA/RNA-binding protein KIN17 supports esophageal cancer progression via resolving noncanonical STING activation induced by R-loop.

Targeting the DNA damage response (DDR) exhibits potent efficacy in inducing immune activation and enhancing patient prognosis. However, the benefits of DDR regulation are not universally observed across all patients, owing to the intricate compensatory mechanisms operative in certain cancers. There still exists a gap in the function of activated DDR protein in esophageal squamous cell carcinoma (ESCC).

Dengue situation in Chinese mainland, 2023.

Background: Dengue fever outbreaks have occurred in Chinese mainland in recent years, especially in 2014 and 2019. In 2023, following the Corona Virus Disease 2019 pandemic, dengue fever has exhibited some new trends and characteristics.

Methods: Descriptive epidemiological methods were used to analyse temporal, spatial and demographic distribution.

Multi-Omics Analysis Reveals Adaptive Strategies of Meconopsis horridula to UV-B Radiation in the Qinghai-Tibet Plateau.

Meconopsis horridula, an endemic medicinal and alpine horticultural species of the Qinghai-Tibet Plateau, exhibits remarkable adaptation to high-altitude UV-B radiation. Despite its ecological and medicinal significance, the mechanisms underlying its UV-B adaptation remain poorly understood. Here, we used a PacBio full-length transcriptome as a reference, integrating RNA-seq and metabolomic data from altitudinal populations, with field-based transcriptomic and microbiome profiling under shade-controlled UV-B gradients, to elucidate UV-B adaptive regulatory networks.

Sustainable production of eicosapentaenoic acid-rich emulsion from Nannochloropsis gaditana by nitrogen regulation and physical cell disruption.

Nannochloropsis gaditana is a promising novel food ingredient; however, its utilization remains limited due to its rigid bilayer cell wall. In this study, co-enrichment of eicosapentaenoic acid (EPA) and protein was achieved by supplementing with 1.76 mmol/L NaNO, yielding biomass enriched with functional nutrients.

Understanding the Degradation and Stress Response Mechanisms of Cypermethrin by J6 Isolated from Broiler Gastrointestinal Tract.

Pyrethroid residues in the environment and agricultural products have caused significant negative effects on food safety and human health. In this study, a novel strain of J6, isolated from the cecum of broilers, exhibited the ability to degrade cypermethrin and its major metabolite, 3-phenoxybenzoic acid. The identified intermediates indicated that J6 degrades cypermethrin through ester bond hydrolysis, benzene hydroxylation, and ether bond cleavage.

Serum Selenoprotein P and Glutathione Expression in Relation to Sperm Kinetic Parameters in Patients with Asthenospermia.

Objective: This study aimed to investigate the relationship amongst serum selenoprotein P (SeP), glutathione (GSH) expression and sperm kinetic parameters in patients with asthenospermia.

Methods: A retrospective analysis was conducted on men of reproductive age who underwent preconception testing at the Center for Reproductive Medicine between October 2022 and October 2024. The study subjects were divided into a case group and a healthy group based on the percentage of progressive motile sperm.

Development of a bioluminescence tomography system using phase measurement profilometry surface reconstruction.

As a widely utilized imaging modality in preclinical studies, bioluminescence tomography (BLT) relies on precise surface reconstruction to provide essential boundary constraints. This study presents a BLT system for small animal imaging that integrates phase measurement profilometry (PMP) to achieve accurate 360° surface reconstruction. The accuracy of surface reconstruction was validated using a cylindrical phantom and live mice.

Plasma microRNA-210 is associated with VEGF-A and EphrinA3 and relates to coronary collateral circulation in patients with coronary heart disease: a cross-sectional study.

Background: This study explored the interrelationships among vascular endothelial growth factor A (VEGF-A), microRNA-210 (miR-210), and EphrinA3 in the plasma of patients with coronary heart disease (CHD), and their collective influence on coronary collateral circulation (CCC) development.

Methods: We enrolled 253 patients with ≥ 90% stenosis in at least one coronary artery, stratified into good CCC (n = 99) and poor CCC (n = 154) groups according to the Rentrop grading system. Plasma concentrations of miR-210, VEGF-A, and EphrinA3 were quantified via qRT-PCR and ELISA.

Kynurenic Acid Is a Predictive Prognostic Metabolic Marker in ST-Elevation Myocardial Infarction.

The tryptophan/kynurenic acid (KYNA) pathway plays a crucial role by modulating inflammation, oxidative stress, and immune activation. The clinical value of tryptophan metabolites in the KYNA pathway for the early diagnosis and prognosis of STEMI patients, as well as the underlying functional mechanisms, remains to be elucidated. This study evaluated the prognostic value of KYNA, a metabolite of the tryptophan pathway, in STEMI patients.

Effect of Chlorine Dioxide Bacteria-Reducing Pretreatment on Quality of Fermented Chili Paste.

Microorganisms play a critical role in determining the quality of fermented chili paste. However, spoilage microorganisms residing on the surface of chili peppers can compromise sensory attributes and reduce edibility. This study aims to adopt chlorine dioxide to reduce spoilage microorganisms prior to fermentation, and the effects on chili paste quality were systematically evaluated.

The Mechanical Role of YAP/TAZ in the Development of Diabetic Cardiomyopathy.

Diabetic cardiomyopathy (DCM) begins with a subclinical stage featuring cardiac hypertrophy, fibrosis, and disrupted signaling. These changes, especially fibrosis and stiffness, often lead to clinical heart failure. The mechanism involves metabolic dysregulation, oxidative stress, and inflammation, leading to cardiac damage and dysfunction.

Association between atrial fibrillation-related symptoms scored by modified European Heart Rhythm Association (mEHRA) and anxious and depressive status.

The key drivers of symptom severity in atrial fibrillation (AF) patients remain unclear. The study aimed to explore associations between anxious and depressive status and AF-related symptoms as expressed by the modified European Heart Rhythm Association (mEHRA) score. The study enrolled 182 AF patients admitted to the Cardiology Department of Peking University Third Hospital between February 2017 to January 2020.

Cell surface Toll-like receptor polymorphisms influence and ectoparasite infections in striped hamsters.

This study explored how polymorphisms in cell surface Toll-like receptors (TLRs) influence susceptibility to and ectoparasite infections in striped hamsters. Among six TLR genes, four sites in TLR1, TLR4, and TLR10 genes were associated with susceptibility, while twelve sites across TLR4, TLR5, and TLR10 contributed to flea resistance. Similarly, eleven sites in TLR5, TLR6, and TLR10 were linked to gamasid mite parasitism.

Using machine learning algorithms to predict risk factors of heart failure after complete mesocolic excision in colorectal cancer patients.

Following complete mesocolic excision (CME), heart failure (HF) emerges as a significant complication, exerting substantial impacts on both short-term and long-term patient prognoses. The primary objective of our investigation was to develop a machine learning model capable of discerning preoperative and intraoperative high-risk factors, facilitating the prediction of HF occurrence subsequent to CME. A cohort comprising 1158 patients diagnosed with colon cancer was enrolled in our study, encompassing 172 individuals who developed postoperative HF.

IbPIF1 confers stem nematode resistance by regulating secondary metabolites in sweet potato.

The stem nematode (Ditylenchus destructor Thorne) is a pervasive and destructive plant-parasitic nematode worldwide, inflicting severe agricultural and economic losses in a wide range of crops. Despite its global impact, the molecular mechanisms underlying plant resistance to this pathogen remain poorly understood. Our previous studies in sweet potato (Ipomoea batatas L.

Antiangiogenic Medications Impede the Oral Mucosal Microcirculation and Interfere with Oral Wound Healing: A Complication Deserving of Attention.

Long-term antiangiogenic therapy may be linked to medication-related osteonecrosis of the jaw (MRONJ), complicating surgical treatment due to impaired postoperative wound healing. However, the mechanisms underlying these healing difficulties remain unclear. This bidirectional cohort study explored the impact of antiangiogenic medications (AGM) in combination with antiresorptive medications (ARM) on oral mucosal microcirculation and its relationship with surgical outcomes in MRONJ patients.

Integrated chemical composition, transcriptomics, and network pharmacology to reveal the mechanism of Jia-Wei-Si-Miao-Yong-An Decoction in ACS model rats.

Background: Acute Coronary Syndrome (ACS), as the most critical pathological type of coronary atherosclerotic heart disease, has a high morbidity and lethality, and the long-term treatment of modern medicine is still faced with the problems of restenosis, microcirculation obstruction and drug resistance. The addition of Traditional Chinese Medicine provides new ideas for ACS treatment. Our previous study found that Jia-Wei-Si-Miao-Yong-An decoction (HJ11) can improve the inflammatory response and cardiac function in ACS.