Machine learning-enhanced digital microscopy for personalized assessment of red blood cell storage lesions.

Background And Objectives: This study aims to develop a novel platform combining machine learning and microscope images for personalized assessment of red blood cell (RBC) storage lesions. RBCs undergo storage lesions, which adversely affect transfusion outcomes. Currently, there is no individualized assessment method for RBC aging applicable in clinical practice.

Metagenomic Analysis of the Tonsil Virome Highlights Its Diagnostic Potential for Rheumatoid Arthritis.

Rheumatoid arthritis (RA) is a chronic autoimmune disease whose exact pathogenesis remains unclear, despite links to genetics, environmental factors, and microbial dysbiosis. Recent studies have highlighted the role of the microbiome in RA, yet the contribution of the tonsil virome remains unexplored. This study aims to investigate whether changes in the tonsil virome are associated with RA progression and assess its diagnostic potential.

TUBB3/DTX LNPs Dry Powder Inhaler and an Efficient Delivery Device for Targeted Therapy of Lung Cancer.

Lung cancer is the most prevalent and deadly tumor globally, marked by drug resistance and a poor prognosis. Specialized formulations with strong lung targeting are currently lacking. Docetaxel (DTX) and TUBB3-siRNA represent chemotherapy and RNA interference (RNAi), two strategies for lung cancer treatment.

Association Between Polymorphisms and Cancer Risk: An Updated Meta-Analysis.

A growing number of studies are exploring the association between HOTAIR rs920778 polymorphisms and cancer risk, but to date, there has been controversy and uncertainty. Preliminary evidence suggests that this polymorphism may influence cancer susceptibility, particularly in Asian populations and specific cancer types such as cervical cancer (CC) and breast cancer (BC). We therefore conducted an updated meta-analysis to accurately assess the association of the HOTAIR rs920778 polymorphism with cancer risk.

Activation of GLP-1 receptors enhances the osteogenic differentiation process of STRO-1-positive BMSCs.

Background: Osteoporosis is a prevalent metabolic bone disorder, especially in individuals with diabetes. Recent studies suggest that GLP-1 plays a positive role in regulating bone metabolism. However, the impact of GLP-1R on bone marrow-derived mesenchymal stem cells, particularly STRO-1-positive BMSCs, remains unclear.

Icariin-loaded composite scaffold promotes osteogenic differentiation and bone regeneration.

Background: Bone defects remain a significant challenge in orthopedics, and traditional treatments often face limitations. Icariin (ICA) has been shown to promote osteogenic differentiation and angiogenesis, which may benefit bone repair.

Methods: ICA-loaded microspheres were prepared using an evaporation method with a co-solvent system.

Hepatoprotective effects of dandelion against AFB-induced liver injury are associated with activation of bile acid-FXR signaling in chicks.

This study aimed to investigate the protective effects of dandelion against AFB-induced hepatotoxicity through the regulation of the FXR signaling pathway in chicks. A total of 144 one-day-old male broilers were randomly assigned to three groups and received a basal diet (BD), and BD supplemented with 0.5 mg/kg of AFB or 0.

Two Birds with One Stone: Empowering Probiotic with Nanoenzyme for the Treatment of Inflammatory and Anemia through Oral Administration.

In the context of ulcerative colitis (UC), iron deficiency anemia (IDA) is often presented as a prevalent systemic manifestation. However, there is an absence of an effective strategy for the specific case of UC with IDA. Herein, mendelian randomization (MR) analysis is applied to confirm the causal association between UC and iron-related conditions.

Five glutathione S-transferase isozymes played crucial role in the detoxification of aflatoxin B in chicken liver.

Background: AFB-8,9-exo-epoxide (AFBO) is the highly toxic product of Aflatoxin B (AFB). Glutathione S-transferases (GSTs) play pivotal roles in detoxifying AFB by catalyzing the conjugation of AFBO with glutathione (GSH). Although there are over 20 GST isozymes that have been identified in chicken, GST isozymes involved in the detoxification process of AFB have not been identified yet.

Clinical and Electrocardiographic Characteristics in NSTEMI Patients With Acute Total Occlusion of Culprit Left Circumflex Artery.

Background: Complete blockage of the culprit coronary artery is associated with 30% of NSTEMI (non-ST-segment elevation myocardial infarction) patients. The culprit vessel in the left circumflex artery (LCX) is more prevalent in this subset of individuals. These people's clinical features and ischemia alterations on electrocardiography (ECG) are unknown.

Butyric acid-based products, alone or in combination with hydroxy-selenomethionine, improve performance of laying hens in post-peak period by modulating their antioxidant, metabolic and immune status.

This study aimed to evaluate the effects of butyrate, butyric glycerides (BG) and their combination with sodium selenite (SeNa) or hydroxy-selenomethionine (OH-SeMet) on the performance and egg quality of the laying hens in post-peak period as well as the potential mechanism. A total of 900 45-week-old Hy-Line brown laying hens were randomly allocated to 5 treatment groups (n = 10 replicates/diet, 18 hens/replicate). The hens were fed the basal diet supplemented with 0.

Effect of time-restricted feeding and caloric restriction in metabolic associated fatty liver disease in male rats.

Background: The prevalence of metabolic associated fatty liver disease (MAFLD) is high. However, there are few studies on the effects of time-restricted feeding (TRF) and caloric restriction (CR) in MAFLD.

Objectives: To investigate the efficacy and mechanism of 4 h TRF and 60% CR in MAFLD.

Chlorine-Tolerant Chlorobenzene Combustion over Mullite Catalysts via Constructing Ru-O-Mn Sites.

The catalytic combustion of chlorine-containing volatile organic compounds (CVOCs) at low temperatures still faces chlorine poisoning challenges. Herein, chlorine-tolerant chlorobenzene combustion over manganese-based mullite (SmMnO) catalysts has been originally demonstrated via constructing rich Ru-O-Mn sites, engineered from the doping of ruthenium (Ru) and the subsequent etching of samarium (Sm). Such catalysts exhibited 90% activity for chlorobenzene combustion at 258 °C and maintained about 80% activity after the 30 h stability test.

A metagenome-wide study of the gut virome in chronic kidney disease.

Chronic kidney disease (CKD) is a progressively debilitating condition leading to kidney dysfunction and severe complications. While dysbiosis of the gut bacteriome has been linked to CKD, the alteration in the gut viral community and its role in CKD remain poorly understood. Here, we characterize the gut virome in CKD using metagenome-wide analyses of faecal samples from 425 patients and 290 healthy individuals.

Characterization and functionality of 1003 isolated from chicken cecum against .

Lactic acid bacteria are widely regarded as safe alternatives to antibiotics in livestock and poultry farming and have probiotic potential. () is a prominent component of pigeon crop microbiota; however, its function is unknown. In this study, a strain of 1003 from pigeon cecum was identified by combining whole genome sequencing and phenotypic analysis, and its safety and probiotic properties were studied.

One Health Approach of Enterococcal Population Structure and Antibacterial Resistance Along the Food Chain - Four PLADs, China, 2015-2022.

Introduction: Enterococci are considered opportunistic pathogens. However, they can serve as a reservoir of antibacterial resistance (ABR) traits and transfer these to humans through the food chain.

Methods: Antibiotic susceptibility testing and multilocus sequence typing were used to characterize the ABR and population structure of 488 enterococcal isolates recovered along the food chain from four provincial-level administrative divisions (PLADs) in China.

Identification of immunity- and ferroptosis-related signature genes as potential design targets for mRNA vaccines in AML patients.

Immune-associated ferroptosis plays an important role in the progression of acute myeloid leukemia (AML); however, the targets that play key roles in this process are currently unknown. This limits the development of mRNA vaccines based on immune-associated ferroptosis for clinical therapeutic applications. In this study, based on the rich data resources of the TCGA-LAML cohort, we analyzed the tumor mutational burden (TMB), gene mutation status, and associations between immune and ferroptosis genes to reveal the disease characteristics of AML patients.

A promising strategy to improve the stability and immunogenicity of killed but metabolically active vaccines: low-temperature preparation and coating of nanoparticles.

Bacteria are becoming an increasingly serious threat to human health. The emergence of super bacteria makes clinical treatment more difficult. Vaccines are one of the most effective means of preventing and treating bacterial infections.

NGF-BMSC-SF/CS composites for repairing knee joint osteochondral defects in rabbits: evaluation of the repair effect and potential underlying mechanisms.

Background: With the rapid growth of the ageing population, chronic diseases such as osteoarthritis have become one of the major diseases affecting the quality of life of elderly people. The main pathological manifestation of osteoarthritis is articular cartilage damage. Alleviating and repairing damaged cartilage has always been a challenge.

Case report: A case of renal vessel rupture caused by severe vomiting in early pregnancy.

Objective: Spontaneous renal vessel rupture is a rare clinical emergency. However, pregnancy symptoms and signs are not obvious, and the limited examination methods obscure the observation. Thus, early renal rupture is challenging to detect, leading to misdiagnosis and poor prognosis.

Gas-Phase Regeneration of Metal-Poisoned VO-WO/TiO NH-SCR Catalysts via a Masking and Reconstruction Strategy.

Renewing metal-poisoned NH-SCR catalysts holds great potential for mitigating environmental pollution and utilizing hazardous wastes simultaneously. Ionic compounds containing heavy metals often exhibit limited solubility due to their high polarizability, making traditional washing techniques ineffective in removing heavy metal poisons. This study presents a gas-based method for regenerating heavy-metal-poisoned VO-WO/TiO catalysts employed in NH-SCR techniques.

Identification of immune- and oxidative stress-related signature genes as potential targets for mRNA vaccines for pancreatic cancer patients.

Adenocarcinoma of the pancreas (PAAD) is one of the deadliest malignant tumors, and messenger ribonucleic acid vaccines, which constitute the latest generation of vaccine technology, are expected to lead to new ideas for the treatment of pancreatic cancer. The Cancer Genome Atlas-PAAD and Genotype-Tissue Expression data were merged and analyzed. Weighted gene coexpression network analysis was used to identify gene modules associated with tumor mutational burden among the genes related to both immunity and oxidative stress.

Feasibility and safety of cryoballoon ablation for atrial fibrillation and closing patent foramen ovale without implantation: A pilot study.

Background: Patent foramen ovale (PFO) affects 20%-34% of adults and is associated with strokes and other disorders. The conventional treatment of PFO-related strokes is a closure procedure. The metal device is associated with some adverse events.