Abnormally Elevated PKCδ Delays Diabetic Wound Healing by Inhibiting the GAD1-GABA Pathway.

Background: Diabetic foot ulcer (DFU) represents a challenging complication of diabetes mellitus, characterized by slow healing processes. Protein kinase C delta (PKCδ) has been identified as a significant factor in the pathogenesis of various diabetic complications, including DFU. However, the precise underlying mechanisms remain to be fully elucidated.

Parkin Acetylation-Mediated Mitophagy Orchestrates Periodontal Ligament Stem Cell Osteogenesis and Bone Regeneration During Ageing.

Aim: To investigate the functional significance of mitophagy in age-related osteogenic decline and the underlying mechanisms using in vivo and in vitro models.

Materials And Methods: An alveolar bone defect model in aged mice and a serial passaging-induced ageing model of human periodontal ligament stem cells (PDLSCs) were established. Osteogenic potential in mice was assessed by micro-CT, immunofluorescence, immunohistochemical analyses and histological staining.

Strong Trapping Capability and Diffusion Suppression Effect of Silicon Carbide Surface on Tritium: A First Principle Study.

Silicon carbide (SiC) has attracted considerable interest for use in electronics, aerospace, and nuclear energy applications owing to its excellent electrical and mechanical properties. In the nuclear industry, SiC serves as an effective tritium permeation barrier. However, a significant discrepancy remains between the experimentally measured diffusion coefficients and the theoretical predictions.

Bio-ethical issues of research in Sub-Saharan Africa.

This paper examines bioethical considerations of research conducted in Sub-Saharan Africa (SSA), where a notable scarcity persists in literature addressing region-specific bioethical issues. Although bioethics-related activities have encountered challenges surpassing existing protocol safeguards, emerging evidence demonstrates growing recognition of integrated scientific and ethical principles within African medical research. Maintaining research continuity in resource-limited settings necessitates bridging critical gaps between informed consent procedures and participants' actual understanding.

Insights Into the Separate and Joint Effects of Cadmium and Cesium on the Risk of Circadian Syndrome and the Underlying Mechanism: An Integrated Epidemiological and Network Toxicological Study.

The uncharted effects of cadmium and cesium on circadian syndrome (CircS), an emerging circadian rhythm disorder drawing considerable attention, and underlying mechanisms warrant exigent elaboration. Data of 11141 subjects from National Health and Nutrition Examination Survey 2005-2018 were incorporated to investigate separate-, joint-/interaction-, and mixture-effects of urinary cadmium and cesium on prevalent CircS risk exploiting survey weight regression and quantile g-computation. The underlying mechanisms were probed by network toxicological analysis.

Nlgalectin mediates insecticide susceptibility in Nilaparvata lugens via modulation of bacterial symbiont.

Galectins are a family of carbohydrate-binding proteins known to maintain intestinal microbiota homeostasis. Emerging evidence suggests that the bacterial symbiont plays a role in modulating insecticide resistance in insect. However, whether galectins influence insecticide susceptibility through microbiota regulation remains unclear.

Host-microbe synergy in pesticide resilience: Rhodococcus-driven fitness compensation in chlorpyrifos-stressed Binodoxys communis.

Chlorpyrifos (CPF), a widely used organophosphate insecticide in cotton cultivation for controlling Aphis gossypii, has Binodoxys communis as the primary parasitic natural enemy of A. gossypii. This study evaluated the impact of two sub-lethal CPF concentrations (LC10 and LC30) on key biological parameters across two generations, transcriptomic responses, and symbiotic bacterial communities in B.

Enhancing D retention index accuracy: Correcting D retention time shifts in GC×GC due to modulation timing deviations via line detection technology.

Even small deviations in modulation timing (DMT) can cause discrepancies between the originally set modulation period (P) and the actual P in comprehensive two-dimensional gas chromatography (GC × GC) analysis. This study explored the impact of DMT on the accuracy of the second dimensional retention time (t) and the second dimensional retention index (I) calculations and introduced a line detection technology (LDT) based on the Hough Transform to correct DMT-induced t shifts. The correction was achieved by automatically adjusting candidate P values until the slope of the line(s) (CB_line(s)) formed by column bleeding compound peaks in the isothermal section of the contour plot approached zero, thereby determining the actual P.

Biogas slurry impregnation-driven hydrothermal synthesis of artificial humic acid: Mechanistic insights and agricultural implications.

In response to the challenges of nutrient limitations and low efficiency in synthesizing artificial humic acid (AHA) during the resource utilization of agricultural wastes, this study innovatively developed a process that integrates biogas slurry (BS) impregnation pretreatment with hydrothermal humification (HTH). Using steam-exploded corn straw (SES) as the raw material, the impregnation parameters were optimized (40 °C, liquid-to-solid ratio of 15:1, 18 h, 3 cycles), achieving an AHA yield of 40.61 %, which was over 15 % higher than that of the untreated group.

Phosphogypsum and Carbide Slag Synergy for Red Mud Soil Stabilization: Mechanical Performance, Environmental Impacts, and Micro-scale Mechanisms.

The production of phosphogypsum (PG), calcium carbide slag (CS), and red mud (RM) in global industrial development imposes serious environmental issues. Utilizing CS and PG as curing agents and incorporating RM as a soil substitute can facilitate the solid waste resource utilization. However, few studies have investigated the synergistic effects of PG and CS on the stabilization of RM and soil.

Jiao-tai-wan and Its Bioactive Constituent Jatrorrhizine Exert Antidepressant Effects via the STING Pathway.

Ethnopharmacological Relevance: Jiao-tai-wan (JTW) is a classical traditional Chinese medicine formula that has long been used to treat insomnia. Recent pharmacological studies have highlighted its potential antidepressant effects. However, its role in regulating neuroinflammation associated with depression and the underlying mechanisms remains unclear.

Small extracellular vesicles orchestrated pathological communications between breast cancer cells and cardiomyocytes as a novel mechanism exacerbating anthracycline cardiotoxicity by fueling ferroptosis.

Small extracellular vesicles (sEVs) critically orchestrate inter-tissue and inter-organ communications and may play essential roles in heart-tumor interaction. However, whether cancer-secreted sEVs affect the progression of doxorubicin-induced cardiotoxicity (DOXIC) via orchestrating the tumor cell-cardiomyocyte crosstalk has not yet been explored. Herein, we reveal that Doxorubicin (DOX)-treated breast cancer cells secrete sEVs (D-BCC-sEVs) that exacerbate DOX-induced ferroptosis of human iPSC-derived cardiomyocytes (hiCMs).

Low-Energy Femtosecond Laser Lens Fragmentation Techniques for Cataract.

Femtosecond laser has become an important tool in cataract surgery due to its precision and minimally invasive nature. However, the strong scattering properties of the lens limit surgical efficiency, especially when it comes to effectively cutting highly opaque and hard cataracts with femtosecond laser. This study proposes a method for achieving optical transparency in opaque lenses through tartrazine treatment.

The long noncoding RNA SNHG12 defines KEAP1 stability and ferroptosis susceptibility by targeting E3 ligase TRIM25.

Ferroptosis is a novel type of programmed cell death caused by iron-dependent lipid peroxidation. Targeted induction of ferroptosis holds great promise for cancer treatment. SNHG, a newly recognized lncRNA family, has been reported to implicate in the proliferation, invasion, migration or drug resistance of cancer cells.

Dissolved organic nitrogen and phosphorus derived from different cyanobacteria regulate distinctly different nitrate reduction pathways in sediments.

The differentiation of the nitrate reduction pathway is of great significance in the ecosystem, as it determines the occurrence form of ecosystem N. In order to explore the impact and mechanism of different algal dissolved organic nitrogen (DON) and dissolved organic phosphorus (DOP) on differentiation of nitrate (NO-N) reduction pathway, small-scale enclosure experiment was conducted to analyze the DON and DOP composition, nutrient level, microbial community composition and NO-N reduction pathway in ponds with Microcystis and Dolichospermum blooms. The main DON produced by Microcystis included lipids and proteins as well as carbohydrate which were readily degradable, whereas the DOP produced by Dolichospermum predominantly consists of readily degradable forms such as carbohydrate and protein.

Microplastic Diversity as a Potential Driver of Soil Denitrification Shifts.

Microplastics (MPs) are raising significant global concerns due to their environmental impacts. While most studies have focused on the effects of individual MP types, MPs in natural environments typically coexist as multiple types, and their combined effects remain poorly understood. In this study, we conducted a microcosm experiment with four levels of MP diversity (0, 1, 3, and 5 types) to investigate the effects of MP diversity on soil ecosystem functions using metagenomic sequencing.

Haplotype-resolved genome and multi-omics landscape reveal an epigenetic regulation of citric acid accumulation in lemon.

Lemon (Citrus limon L.), an economically important Citrus species, produces high levels of citric acid. However, the regulatory mechanisms underlying citric acid accumulation in lemon fruit are poorly understood.

Hybrid epigenome unveils parental genetic divergence shaping salt-tolerant heterosis in Brassica napus.

Heterosis holds great potential for improving yield, quality, and environmental adaptability in crop breeding, which suggests that hybrids can exhibit better performance in adapting to extreme environments. However, the epigenetic mechanisms of salt-tolerant heterosis in allopolyploid crop Brassica napus (AACC, 2n = 38), particularly chromatin accessibility, remain largely unexplored. We investigated the dynamics of chromatin accessibility and transcriptional reprogramming during a time course of salt exposure in Brassica napus hybridization.

Development and validation of a nomogram for predicting 30-day outcomes in patients with unruptured vertebrobasilar dissecting aneurysms undergoing endovascular treatment: a multicenter cohort study.

Background: Endovascular treatment (EVT) of vertebrobasilar dissecting aneurysms (VBDAs) is associated with high morbidity and mortality, significantly influencing patient prognosis. This study aimed to develop and validate a nomogram for predicting 30-day outcomes in patients with unruptured VBDAs undergoing EVT.

Methods: This retrospective study included 606 patients with unruptured VBDAs who underwent EVT at 10 institutions between January 2015 and April 2025, with 491 in the training cohort and 115 in the validation cohort.

Nuclear cGAS mediated Replication Stress and Mitotic Catastrophe can Overcome Gemcitabine Resistance.

Gemcitabine, a ribonucleotide reductase (RNR) inhibitor, is active in pancreatic ductal carcinoma (PDAC) patients, but unfortunately has a limited impact on long term outcomes. Gemcitabine induces nucleotide deficiency, DNA damage including single stranded DNA (ssDNA) and replication stress (RS). DNA damage can activate cyclic GMP-AMP synthase (cGAS), leading to genome instability, micronucleus generation, and immune activation.

Prevalence and risk factors of postpartum depressive symptoms in Central China: A longitudinal, real-world study.

Background: Postpartum depressive symptoms is a common and serious mental disorder. Most of the previous studies on the predictors of postpartum depressive symptoms adopted cross-sectional designs with relatively low levels of research evidence. Based on a prospective longitudinal study design, this study aimed to investigate the prevalence of postpartum depressive symptoms and associated risk factors in central China.

Volatile organic compounds exposure with risk of thyroid function in U.S. general population: 2007-2012 NHANES.

Background: The link between exposure to environmental hazards and thyroid function has been relatively well established. However, evidence on environmental exposure to volatile organic compounds (VOCs) and thyroid function in the general population remains unclear.

Objectives: To assess the impact of individual and joint effects of VOCs exposure on thyroid function in a representative sample of U.

Comparative analysis of endplate volumetric bone mineral density and endplate vertebral bone quality for predicting cage subsidence in lateral lumbar interbody fusion.

Objective: The aim of this study was to compare the predictive efficacy of quantitative CT (QCT)-based endplate volumetric bone mineral density (EP-vBMD) and MRI-based endplate vertebral bone quality (EBQ) score for cage subsidence (CS) after lateral lumbar interbody fusion (LLIF).

Methods: A retrospective study was conducted on patients who underwent single-level LLIF in conjunction with pedicle screw fixation at the authors' institution between January 2019 and April 2023. The volumetric bone mineral density (vBMD) was measured based on preoperative CT using phantom-less QCT software.