An IFE-based molecularly imprinted nanosensor for highly sensitive and rapid ratiometric fluorescence-visualization detection of ciprofloxacin.

A versatile fluorescent molecularly imprinted nanosensor (MIPs@O-CDs) for profiling ciprofloxacin (CIP) was innovatively developed using a controllable post-imprinting modification strategy. High-affinity molecularly imprinted polymers (MIPs) as recognition elements granted nanosensor favorable anti-interference. Bright orange-emission carbon dots (O-CDs) as signal transducers demonstrated prominent reverse fluorescence response to CIP due to inner filter effect, ameliorating detection sensitivity and accuracy.

Characterization of peptide-enriched walnut meal paste via microbial diversity and metabolite profiling.

Walnut meal (WM), a protein-rich byproduct of oil extraction, holds great potential for conversion into value-added food products but remains underutilized. In this study, a two-stage fermentation process involving Aspergillus oryzae inoculation for koji preparation followed by natural brine fermentation was developed to produce a peptide-enriched walnut meal paste (WMP). Physicochemical analysis showed significant increases in total acidity (0.

ZmDof08, a zinc finger transcription factor, plays critical roles in photosynthesis in maize.

The gene ZmDof08, which underlies the yellow-green leaf mutant phenotype in maize, enhances the activity of key enzymes involved in C photosynthesis, leading to a significant improvement in photosynthetic efficiency. Improving the photosynthetic efficiency of maize to increase its yield has long been a key focus in global agricultural research. Maize possesses a rich resource of leaf color mutants, which serve as valuable materials for studying leaf photosynthesis.

Development and application of a dual RT-qRPA assay for rapid differentiating of goose astrovirus genotypes 1 and 2.

Goose astrovirus (GAstV) is an important pathogen causing joint and visceral gout in goslings. Its high incidence and mortality had caused enormous economic losses to the goose farming industry. To achieve the simultaneous and rapid identification and detection of Goose astrovirus genotype 1 (GAstV-1) and genotype 2 (GAstV-2), the present study aimed to design and synthesize specific recombinase polymerase amplification (RPA) primers and exo probes targeting the ORF1b gene of GAstV.

Screening of essential oils with acaricidal activity against the poultry red mite (Dermanyssus gallinae) and analysis of active components.

Dermanyssus gallinae is a common ectoparasite in the laying hen industry, damaging chicken health by sucking blood. With mites developing acaricide resistance, there's an urgent need for alternatives. In this study, we evaluated the efficacy of 34 essential oils (EOs) on D.

Oviposition Response to Light With Different Spectra in the Crepuscular Moth .

The light spectrum is a critical visual feature influencing insect behavior. The crepuscular moth (Busck), a significant pest of stone and pome fruits worldwide, has been shown to discriminate variations in brightness/intensity under dim-light conditions. However, the behavioral responses of females to various light spectra remain unknown.

Peanut oil is more environmentally sustainable than rapeseed oil from a carbon and nitrogen footprint perspective in China.

Peanut and rapeseed oil, prominent edible oils in China, significantly contribute to greenhouse gas and reactive nitrogen emissions. A comprehensive examination of their environmental footprints is foundational for developing green and low-carbon products. Using a cradle-to-factory gate life cycle assessment, we quantified the carbon footprint (CF) and nitrogen footprint (NF) associated with the oil production of peanut and rapeseed from 2004 to 2023 in China.

The influence of different emulsifiers on dotted gizzard Shad (Konosirus punctatus) oil emulsions.

This study investigates the effects of chitosan quaternary ammonium salt (HACC), carrageenan (CA), and lecithin (LC) on soy protein isolate (SPI). SPI is a widely used natural emulsifier but is highly susceptible to environmental perturbations; the incorporation of additional bioactive components can modulate its structural integrity and functional stability. For the first time, the physicochemical properties, microstructure, and in vitro digestibility of previously reported SPI complexes were compared.

Light incubation affects embryo development and post-hatch performance in poultry.

Light is one of the important environmental factors affecting the growth, reproduction, and performance of poultry. It stimulates retinal photoreceptor cells to form visual imaging, enabling animals to adapt to external environments. Light also regulates non-visual physiological responses such as circadian rhythms, pupillary reflex, sleep regulation, and melatonin secretion.

Legacy Effects of Different Preceding Crops on Grain Yield, Protein Fractions and Soil Nutrients in Subsequent Winter Wheat.

Given the pressing global food security crisis and climate change-induced constraints on agricultural productivity, crop rotation proves critical for boosting yield and grain quality of winter wheat () alongside ameliorating soil quality. However, the legacy effect of different preceding crops on synergistic increments of wheat productivity and soil fertility remains to be fully clarified. Five different preceding crop-winter wheat rotations were conducted in a field experiment established in Huanghua, China.

Analysis of Insect Resistance and Ploidy in Hybrid Progeny of Transgenic Triploid Poplar 741.

With the increasing severity of forest pest problems, breeding insect-resistant varieties has become a crucial task for the sustainable development of forestry. The highly insect-resistant triploid Populus line Pb29, genetically modified with , served as the maternal parent in controlled hybridization with three paternal Populus cultivars. Hybrid progenies were obtained through embryo rescue and tissue culture.

Polysaccharides Alleviate Aflatoxin B1-Induced Liver Damage and Immune Disorders by Inhibiting the ROS-Mediated Mitochondrial Apoptosis Pathway.

polysaccharide (DSPS) is the main natural compound extracted from the traditional Chinese herb . Although DSPS is well-known for its antioxidant and anti-inflammatory properties, its impact on aflatoxin B1 (AFB1)-induced damage has not been explored. This study aims to investigate the potential protective mechanisms of DSPS against AFB1-induced liver damage and immune disorders.

Naringenin Inhibits Enterotoxigenic -Induced Ferroptosis via Targeting HSP90 in IPEC-J2 Cells.

Enterotoxigenic (ETEC) leads to severe diarrhea in piglets. Naringenin (Nar), a natural flavonoid compound, is known for its antibacterial and anti-antioxidant properties. However, the protective effects of Nar against ETEC-induced diarrhea have not been reported yet.

Association between Weaning Stress and Rumen Microbiota in Goat Kids: Evidence from Granger Causality and Randomized Controlled Trial Validation.

Objective: Weaning stress is frequently associated with disrupted gastrointestinal microbiota and decreased growth performance, the association between them remains unclear.

Methods: In this study, we employed Granger causality inference to compare the dynamic changes in gastrointestinal microbiota between stressed and non-stressed goat kids, followed by validation through a randomized controlled trial involving rumen microbiota transplantation.

Results: Our findings indicated that the alpha diversity and microbial maturity of the rumen microbiota in stressed kids were significantly lower than those in non-stressed kids during the weaning period (p<0.

Research advancements in deep eutectic solvent pretreatment technologies and bioconversion of straw biomass.

Straw pretreatment technology and high-value usage are critical linkages in the current sustainable development of agriculture and the achievement of dual-carbon targets. Using effectivepretreatment technology to break down the complicated structure of straw biomass, followed by biotransformation via the action of hydrolases and microorganisms, is the critical link in the high-value usage of straw lignocellulose. In recent years, the development and application of biorefinery technology have created new prospects for the conversion of green, efficient, and low-cost straw biomass.

Direct Ser797 Interacted Pteridine-7(8)-one Derivatives as Highly Selective and Orally Available EGFR Inhibitors.

The EGFR mutation is the main mechanism of acquired resistance to Osimertinib in NSCLC. There is an urgent need for small-molecule inhibitors to overcome Osimertinib-resistance. In this article, we developed a series of pteridin-7(8)-one-derived inhibitors of EGFR (EGFR) integrating direct interactions with the mutated Ser797 residue and the hydrophobic pocket encircled by the gatekeeper Met790 residue.

Nitrogen fertilizer improves growth and soil qualities.

Introduction: Soil contamination with heavy metals (e.g., Pb, Cd) poses severe environmental risks due to industrialization.

Functional epitope mapping of porcine reproductive and respiratory syndrome virus nsp7α and nsp7β using monoclonal antibodies and development of a feasible ELISA.

Porcine reproductive and respiratory syndrome virus (PRRSV) poses a significant threat owing to its considerable economic impact on the global swine industry. Nonstructural protein 7 (nsp7) plays an essential role in inducing the humoral immune response, resulting in the production of high and persistent antibodies in PRRSV-infected pigs. Therefore, nsp7 is considered the most appropriate marker for diagnostic development.

Modulation mechanism of dextran-mediated glycation on soy protein isolate-quercetin interaction: a spectroscopic and molecular docking study.

This study systematically investigated the interaction mechanisms between glycated soybean protein isolate (GSPI) formed from soy protein isolate (SPI) and dextran (DEX) via Maillard reaction, and quercetin (Que), employing multi-spectroscopic analysis and molecular docking simulation. The results indicated that hydrogen bonding and van der Waals forces dominated the non-covalent binding between GSPI and Que, with the Maillard reaction further enhancing hydrogen bonding interaction. This enhancement was substantiated through molecular docking simulations, which revealed short hydrogen bonds (< 3.

Isolation of porcine intestinal Lactobacillus salivarius and antimicrobial potential of its biosurfactant.

Background: Lactobacillus salivarius (L. salivarius) is a probiotic widely colonized in the gastrointestinal tracts of humans and animals. Its biosurfactant (BS) exhibits antibacterial and antiviral activities, along with advantages such as biodegradability, non-toxicity, and stability.

Influences of hydrological and agricultural conditions on selenium distribution in groundwater: A case study in Sanjiang plain, Northeast China.

Understanding selenium (Se) distribution mechanisms in groundwater from farmlands is crucial for ensuring water resource safety. In this study, the distribution patterns and sources of Se in the groundwater of Bawuer, Daxing, and Xinglonggang farmlands within the Naoli River Basin (Sanjiang Plain, Northeast China) were analyzed through comprehensive analyses of 154 groundwater samples and 200 soil/sediment samples from boreholes in the farmlands. Isotopic data of δD and δO demonstrated that precipitation and river recharge are the primary sources of groundwater.

Transcription Factor TrStuA Regulates Vegetative Growth, Virulence, and Trichothecin Synthesis in .

, a postharvest apple pathogen, produces the mycotoxin trichothecin (TCN), which poses a significant threat to human health. However, the regulatory function on TCN synthesis in is poorly understood. In this study, we characterized functions of the APSES-type transcription factor StuA in .

Advanced imaging-enabled understanding of cell wall remodeling mechanisms mediating plant drought stress tolerance.

Drought stress causes peculiar challenges to plant cells reliant on turgor pressure and a polysaccharides-enriched cell wall for growth and development. Appropriate cell wall changes in mechanical properties and biochemical composition under stress conditions constitute an indispensable stress adaptation strategy. A better understanding of stress-induced cell wall modifications is not only crucial for accruing fundamental scientific knowledge in plant biology, but will help us design novel strategies for enhancing crop drought tolerance.

A maturity classification model for winter jujubes based on DSAF-ResNet.

Accurate, non-destructive classification of winter jujube maturity is critical for quality control and intelligent harvesting. This study proposes a dual-stream attention-fused residual network (DSAF-ResNet) combining hyperspectral and GLCM-based texture features at the feature level. The multimodal fusion significantly improved classification performance, with ResNet34 achieving 92.

Fingerprinting the Intestinal Transport of Low-Molecular-Mass Advanced Glycation End-Products (AGEs) Using a Caco-2 Transwell Model.

Food-borne advanced glycation end-products could potentially contribute to the endogenous AGE accumulation within the body, albeit to a different extent for different AGEs. This study focuses on characterizing intestinal absorption and intracellular accumulation of 10 selected free low-molecular-mass (LMM) advanced glycation end-products (AGEs) to obtain insight into potential differences in their systemic bioavailability by using a Caco-2 transwell model. The findings reveal that all tested AGEs can cross the intestinal barrier through the paracellular route, albeit to a limited extent.