Extraintestinal pathogenic Escherichia coli (ExPEC) O-serotype distribution and antibiotic resistance profiles among the aging population in China: A longitudinal, multi-center study.

Extraintestinal pathogenic Escherichia coli (ExPEC) is a leading cause of community-acquired bacteremia and sepsis, which contributes to the substantial burden of invasive E. coli disease (IED) in older adults. This study aimed to estimate the O-serotype distribution of blood and sterile site ExPEC among older adults in China and the characteristics of antimicrobial resistance, O-serotypes, and O genotypes.

Robust Interfacial Hydrogen-Bond Network on Positively Charged Ru-N-Ni Dual Sites Boosts Alkaline Hydrogen Electrocatalysis.

Ruthenium (Ru)-based dual-site catalysts can efficiently accelerate alkaline hydrogen electrocatalytic kinetics by virtue of the well-balanced competitive adsorptions of multiple reaction intermediates. However, their insufficient mass transfer makes them far away from the applications, largely lying to the challenge of precisely manipulating the interface water structure. Herein, a concept of nitrogen-bridged positively charged dual sites with a robust interfacial hydrogen-bond network is presented for enhancing alkaline hydrogen oxidation and evolution reactions (HOR and HER).

Association between Weaning Stress and Rumen Microbiota in Goat Kids: Evidence from Granger Causality and Randomized Controlled Trial Validation.

Objective: Weaning stress is frequently associated with disrupted gastrointestinal microbiota and decreased growth performance, the association between them remains unclear.

Methods: In this study, we employed Granger causality inference to compare the dynamic changes in gastrointestinal microbiota between stressed and non-stressed goat kids, followed by validation through a randomized controlled trial involving rumen microbiota transplantation.

Results: Our findings indicated that the alpha diversity and microbial maturity of the rumen microbiota in stressed kids were significantly lower than those in non-stressed kids during the weaning period (p<0.

Discoidin, CUB and LCCL domain containing 2 modulates angiogenesis by inhibiting VEGF receptor 2 endocytosis in endothelial cells.

The internalization of vascular endothelial growth factor receptor-2 (VEGFR-2) occurs in response to VEGF treatment, and it is eventually transported to the plasma membrane by several endosomes such as Rab5 and Rab11, which are responsible for transporting vesicles from the cytoplasm to plasma membrane. Therefore, the homeostasis of VEGFR-2 internalization and recycling is critical for maintaining the normality of the VEGF signaling pathway and regulates angiogenesis. Previous studies have shown that discoidin, CUB and LCCL domain containing 2 (DCBLD2) can promote the proliferation and migration of vascular endothelial cells (ECs) by promoting the VEGF signaling pathway, but the potential role of DCBLD2 on VEGFR-2 endocytosis remains unclear.

Simple Lifelong Learning Machines.

In lifelong learning, data are used to improve performance not only on the present task, but also on past and future (unencountered) tasks. While typical transfer learning algorithms can improve performance on future tasks, their performance on prior tasks degrades upon learning new tasks (called forgetting). Many recent approaches for continual or lifelong learning have attempted to maintain performance on old tasks given new tasks.

PFKM phosphorylates histone H3 and promotes mitotic progression by sensing the levels of citrate.

Emerging evidence indicates that metabolic signals-including nutrient availability, biosynthetic intermediates, and energy balance-are linked to cell cycle progression. However, how these signals are sensed by the cell cycle machinery remains unclear. Citrate, a key intermediate in the TCA cycle, peaks during mitosis (M phase) and is detected by the glycolytic enzyme ATP-dependent 6-phosphofructokinase 1 muscle isoform (PFKM), accelerating mitotic progression.

Evaluation of the disease burden of nosocomial infection among inpatients in a cancer hospital based on propensity score matching.

Background: Oncology patients, as an immunocompromised population, are particularly susceptible to hospital-acquired infections (HAIs). This study aims to establish a scientifically sound model to estimate the disease burden associated with HAIs in oncology patients, providing valuable decision-making support for healthcare systems and public health management.

Methods: Propensity score matching (PSM) was employed.

Construction and characterization of thiolated chitosan coated TPGSylated nanodiamonds for oral delivery of curcumin.

Low water solubility and poor intestinal permeability hinder the oral absorption of curcumin (CUR). To address this, we designed a core-shell structured nanoparticle based on nanodiamonds (NDs) and thiolated chitosan (TCS). First, D-α-tocopherol polyethylene glycol 1000 succinate (TPGS) covalently modified NDs were prepared and loaded with CUR (CUR@NDs-TPGS).

Regulating a NaF-Rich SEI Layer for Dendrite-Free Sodium Metal Batteries Using Trifunctional Halogenated Covalent Organic Framework Separators.

Uncontrolled sodium-ion (Na) transport, fragile solid electrolyte interphase (SEI) layers, in and sluggish Na desolvation using conventional separators drive dendrite growth, posing critical challenges to the development of sodium metal batteries (SMBs). Porous materials with tunable Na transport pathways offer promise; however, simultaneously enhancing Na kinetics, promoting NaF-rich SEI formation, and lowering desolvation energy barriers remains a critical challenge. Herein, a trifunctional halogenated covalent organic framework (COF) integrated into a polypropylene (PP) separator (COF-F@PP) is designed to address these issues.

The mediating role of body mass index in the association between unprocessed or minimally processed foods and gallstones.

Background: The extent of food processing significantly impacts human health, with ultra-processed foods (UPFs) linked to numerous adverse health outcomes. In contrast, research on unprocessed or minimally processed foods (MPFs) and their association with gallstones remains scarce. This study aimed to investigate the relationship between MPF intake and gallstones in U.

Evaluating the relationship between familial poverty, seropositivity, and all-cause mortality in the general US population.

Purpose: Socioeconomic inequality is closely related to the incidence of () infection and mortality outcomes. Accordingly, this study was designed with the goal of exploring the relationship between familial poverty, seropositivity, and all-cause mortality among adults in the United States.

Methods: Data from National Health and Nutrition Examination Survey (1999-2000) was used to conduct analysis.

The next generation of immunotherapies for lung cancers.

Immunotherapies, specifically immune-checkpoint inhibitors (ICIs) targeting PD-(L)1 or CTLA4, have revolutionized the treatment of lung cancer; however, many patients do not have a response to ICIs and most of those with an initial tumour response eventually have disease progression owing to acquired resistance. Over the past few years, numerous therapeutic strategies have been explored to address the problems of intrinsic and acquired resistance to ICIs. In 2024, regulatory approvals of the bispecific PD-1 × VEGF antibody ivonescimab for the treatment of non-small-cell lung cancer in China and the bispecific DLL3 × CD3 T cell engager tarlatamab for patients with small cell lung cancer in the USA provided clinical proof-of-concept for overcoming the challenge of ICI resistance using novel immunotherapeutic agents, thereby increasing enthusiasm for the exploration of next-generation immunotherapies for lung cancer.

Can phthalate esters be used as tracers for benzotriazole ultraviolet stabilizers in aquatic environments? A case study of the Huangpu River, Shanghai, China.

Benzotriazole ultraviolet stabilizers (BUVSs) and phthalate esters (PAEs), widely employed industrial additives, have raised ecological concerns due to their potential environmental risks. However, their co-occurrence patterns in aquatic systems remain understudied, with their potential correlations poorly characterized. This study investigated the seasonal and spatial distribution patterns of BUVSs and PAEs in the Huangpu River, while evaluating the potential of PAEs as tracers for BUVSs.

Three detection modes with one electrode: A multifunctional electrochemical sensing platform for thrombin, p53 gene, and cholesterol detection.

The development of diverse biomarker detection platforms is crucial to efficient and low-cost assays. Unlike most conventional multi-target methods requiring complex probe labeling and prone to signal cross-talk, a novel multifunctional sensor incorporating host-guest chemistry in electrochemical assays was developed to detect protein, nucleic acid, and small molecule with a single electrode in this work. This sensor operates in three distinct modes: protein detection, nucleic acid detection, and small molecule detection.

Catalytic Ethylene Oligomerization over Imine-Linked Covalent-Organic Frameworks with Coordinative Ni(II) and Cr(III).

Covalent-organic frameworks (COFs) hold great promise for heterogeneous catalysis due to their porous structure for gas adsorption and tunable functionality for bond activation. This study reports two flower-like shaped imine-linked COFs with coordinative Ni-(II) and Cr-(III) species, to endow Ni/PD-TPA COF and Cr/PD-TPA COF, for catalytic ethylene oligomerization. The synergy between imine nitrogen atoms and coordinated metals enables an exceptional activity (16.

Interfacial Engineering and Structural Modulation of RuO-Based Catalysts for Highly Active and Durable Oxygen Evolution Reaction in Acidic Environment.

Developing highly active and durable catalysts for the oxygen evolution reaction (OER) under acidic conditions is key to commercializing green hydrogen production via water splitting. Here, we fabricated a cobalt oxide (CoO)-synergized nickel-doped ruthenium oxide (Ni-RuO) heterojunction on reduced graphene oxide (CoO/Ni-RuO/rGO) as an efficient and durable OER electrocatalyst in acidic electrolytes. The interface of CoO/Ni-RuO heterojunction and doping of Ni into RuO, as well as their effect on electronic structure, were examined by advanced characterizations.

[Comparison of anti-inflammatory, antibacterial and analgesic activities of formulated granules traditional decoction of Powder].

Objectives: To compare the anti-inflammatory, antibacterial and analgesic effects of Powder (YQS) formulated granules and decoction.

Methods: We first evaluated the anti-inflammatory effects of the two dosage forms of YQS in a LPS-induced RAW 264.7 cell model using RT-qPCR and Western blotting.

Functionalization of loofah sponge as a natural cellulose-based material for water environment remediation and sustainability assessment.

Loofah-derived biomass sponges, as a natural, porous, and environmentally friendly material, demonstrate significant potential in the field of water environmental remediation due to their unique three-dimensional interconnected multi-channel structure and superior mechanical properties. The inherent structure of loofah sponges endows them with excellent mechanical strength, hydrophilicity, ion transport capacity, sound absorption properties, adsorption performance, and antibacterial activity. Physical, chemical, and biological modification methods can substantially enhance their adsorption capabilities and mechanical strength.

Oral Acid-Activated Hydrogen-Producing Nanoparticles Reduce Aortic Dissection Progression via RhoA/ROCK Inhibition in Mice.

Aortic dissection (AD) is a life-threatening condition with a high mortality rate. Oxidative stress and endothelial and vascular smooth muscle cell migration contribute to AD pathogenesis. Herein, we investigated the therapeutic potential of hydrogen (H), delivered via magnesium diboride nanosheets (MBNs), in a murine model of β-aminopropionitrile-induced AD.

Bioinformatics analysis of circular RNAs associated with atrial fibrillation and their evaluation as predictive biomarkers.

Background: Circular noncoding RNAs (circRNAs) are implicated in many human diseases, but their role in atrial fibrillation (AF) is poorly understood. In this study, we performed bioinformatics analysis of circRNA sequencing data to identify AF-related circRNAs.

Methods: Left atrial appendage (LAA) samples were obtained from patients with valvular heart disease and were categorised into the sinus rhythm (SR; n = 4) and AF (n = 4) groups.

Synergistic Effect of Boron and Oxygen Coordination on Ruthenium Clusters for Industrial Water Splitting in Alkaline Medium.

The alteration in the coordination environment of metal atoms can manipulate their electronic structure and regulate the electrocatalytic hydrogen evolution activity. In this work, synchrotron radiation tests prove that the boron (B) and oxygen (O) elements co-coordinate with ruthenium clusters (Ru) on the surface of B-O modified reduced graphene oxide. The electrochemical tests demonstrate that this unique structure electrocatalyst presents an overpotential of 12 mV in 1 M KOH condition and for over 120 h at the current of -1 A cm, indicating potential practical applications.

Cryo-EM structures of DNA-free and DNA-bound BsaXI: architecture of a Type IIB restriction-modification enzyme.

We have determined multiple cryogenic electron microscopy (cryo-EM) structures of the Type IIB restriction-modification enzyme BsaXI. Such enzymes cleave DNA on both sides of their recognition sequence and share features of Types I, II, and III restriction systems. BsaXI forms a heterotrimeric (RM)2S assemblage in the presence and absence of bound DNA.

Metagenomic analysis reveals soil microbiome responses to microplastics and ZnO nanoparticles in an agricultural soil.

Both microplastics (MPs) and engineered nanoparticles are pervasive emerging contaminants that can produce combined toxicity to terrestrial ecosystems, yet their effects on soil microbiomes remain inadequately understood. Here, metagenomic analysis was employed to investigate the impacts of three common MPs [i.e.

Internet-based behavioural parent training intervention for children with attention-deficit/hyperactivity disorder: A randomized clinical trial.

Background: Behavioral parent training (BPT) is recognized as a significant non-pharmacological treatment for attention-deficit/hyperactivity disorder (ADHD). However, there is limited empirical evidence of its effectiveness within the Chinese context, particularly for online BPT. This study evaluates the efficacy of an 8-session internet-based BPT (I-BPT) program in improving ADHD symptoms and mental health outcomes for children and parents, compared to routine clinical care (RCC).

Proxi-RIMS-seq2 applied to native microbiomes uncovers hundreds of known and novel m5C methyltransferase specificities.

Methylation patterns in bacteria can be used to study restriction-modification or other defense systems with novel properties. While m4C and m6A methylation are well characterized mainly through PacBio sequencing, the landscape of m5C methylation is under-characterized. To bridge this gap, we performed RIMS-seq2 (rapid identification of methyltransferase specificity sequencing) on microbiomes composed of resolved assemblies of distinct genomes through proximity ligation.